fabiana tara dewi_13.70.0166_kloter a_unika soegijapranata

7/23/2019 fabiana tara dewi_13.70.0166_kloter A_UNIKA SOEGIJAPRANATA

1/20

FIKOSIANIN

LAPORAN RESMI PRAKTIKUM

TEKNOLOGI HASIL LAUT

Disusun oleh:

Fabiana Tara 1!"#!#1$$

Kelo%&o': A(

PROGRAM STUDI TEKNOLOGI PANGANFAKULTAS TEKNOLOGI PERTANIAN

UNI)ERSITAS KATOLIK SOEGI*APRANATA

SEMARANG

+#1(


2/20

1. MATERI DAN METODE

1.1. Materi

,ahan -an. /i.una'an a/alah bio%assa s&irulina basah a0au 'erin. a'ua/es /e's0rin!

Se/an.'an ala0 -an. /i.una'an a/alah s0eri2u.e &en.a/u'3stirrer, ala0 &en.erin.

4o5en6plate stirrer.

1.2. Metode

,io%assa S&irulina /i%asu''an /ala% erlen%e-er

Dilaru0'an /ala% aqua /es0ila0a 41 : 1#6

Dia/u' /en.anstirrer 7 + 8a%


3/20

Disen0ri2u.asi (### r&% 1# %eni0 hin..a /i/a&a0 en/a&an

Su&erna0an /iu'ur 'a/ar 2i'osianin &a/a &an8an. .elo%ban. $1( n% /an $(+ n%

Di0a%bah /e's0rin /en.an su&erna0an : /e's0rin 9 1 : 1


4/20

Dia%&ur %era0a /an /i0uan. 'e ;a/ah

Dio5en &a/a suhu hin..a 'a/ar air 7 "?

Di/a&a0 a/onan 'erin. -an. .e%&al


5/20

Dihanur'an /en.an &enu%&u' hin..a berben0u'powder


6/20

2. HASIL PENGAMATAN

Diliha0 /ari hasil &ra'0i'u% -an. /ila'u'an /i/a&a0'an /a0a seba.ai beri'u0:

Ke0eran.an @arna ,iru Mu/a ,iru ,iru Tua

Kel ,era0 *u%lah ABua/esTo0alFil0ra0 OD

$1(OD$(+

KF Ciel/ @ar

,ioMassaKerin.4.6

-an./i0a%bah'an4%l6

-an./i&eroleh

4%.3%l6 4%.3%l6Sebelu%/iO5en

A1 # (##(on5en0ional an/ In0e.ra0e/ ABueous T;oPhase S-s0e%s Spirulina plantesis

/i8a/i'an 2o'us /ala% /unia bio0e'nolo.i 'arena %e%ili'i nilai e'ono%i -an. 0in..i

e'olo.is ser0a nilai nu0risi! ,an-a' 'e.unaan /ari %i'roal.a ini se&er0i un0u' &e;arna

5i0a%in linoleni ai/ /an eni%! Dala% 2un.sin-a seba.ai &e;arna ;arna -an.

/ihasil'an s0abil ser0a bersi2a0 hi/ro2ili'! Spirulina plantesis %eru&a'an .olon.an

2i'obili&ro0ein /i%ana 0erbe.ai /ala% bebera&a 'elo%&o' -ai0u &h-o-anin

&h-oer-0hrin /an allo&h-o-anin! Kara'0eris0i' 0ia& 'elo%&o' ber.an0un. &a/ains0ensi0as &en-era&an ;arna! Fi'osianin %eru&a'an 'o%&oen u0a%a /ala%

2i'obili&ro0ein 4An0elo et al., +#1#6!

Dari 8urnal E22e0 o2 >arbon >on0en0 Salini0- an/ &H on Spirulina platensis 2or

Ph-o-anin Allo&h-o-anin an/ Ph-oer-0hrin Au%ula0ion /i0a%bah'an bah;a

si2a0 /ari 2i'obili&ro0ein a/alah laru0 air /an %en.an/un. 'a/ar 0in..i &ro0ein!

Fi'obili&ro0ein ber&eran /ala% &e%bua0an &e;arna ala%i hin..a &e;arna sin0e0i' -an.non0o'si' /an /an non'arsino.eni'! Fi'obili&ro0ein 8u.a /a&a0 /i.una'an seba.ai

sis0e% i%un /ala% 0ubuh bai' /isi%&an /ala% 0e%&era0ur %e%ili'i 'oe2esien

absorbansi -an. 0in..i 4Shar%a et al., +#1-ano&h-0a


9/20

Kelas : >-ano&h-eae

Or/o : Nos0oales

Fa%ili : Osilla0oriaeae

Genus : S&irulina

S&esies : Spirulina sp

.a%bar 1! Spirulina sp

4Mussa.- et al., +##$6

3.2. Fikosiai

Fi'osianin %eru&a'an &i.%en -an. %e%beri ;arna biru 0ua /an %e%ili'i 'e%a%&uan

%e%anar'an ;arna %erah 0ua 8i'a 0er'en aha-a! Fi'osianin a/alah &i.%en %a-or

-an. 0er'an/un. &a/a Spirulina plantesis un0u' &en-i%&an ni0ro.en! Pi.%en 2i'osianin

/i'en/ali'an 2i'obili&ro0ein 4Rih%on/ 16! @arna biru &a/a 2i'osianin berasal /ari

'elo%&o' sen-a;a -an. beri'a0an seara 'o5alen &a/a ran0ai 'ro%o2ors 0e0ra&irol

0erbu'a /en.an inin 2i'obilin -an. %enan.'a& ra/i'al o'si.en sehin..a 2i'osianin

/a&a0 %en.ha%ba0 &e%ben0u'an sel 'an'er /ala% 0ubuh %anusia 4A/a%s +##(6!

Fi'osianin 8u.a /a&a0 /i%an2aa0'an seba.ai an0io'si/an an0ira/an. bahan /asar

&er%en 'are0 &er%en ;asabi /an 'os%e0i'a se&er0i lipstick /an eyeliner 4S&olaore et

al., +##$ /an El,a'- +##6! Fi'osianin /a&a0 rusa' &a/a suhu -an. 0in..i sehin..a

&erl &erla'uan -an. benar sehin..a %e%ili'i u%ur si%&an -an. la%a 4Mishra et al.,

+##6!

.a%bar +! S0ru'0ur Ki%ia Fi'osianin

Su%ber >arra hEoha 1"$


10/20

Dari 8urnal Ther%al S0abili0- I%&ro5e%en0 o2 ,lue >oloran0 >Ph-oianin 2ro%

Spirulina platensis 2or Foo/ In/us0r- A&&lia0ions %en8elas'an bah;a rea'si -an.

0er8a/i /ian0ara 'onsen0rasi 0in..i &a/a .ula a'an %enin.'a0'an s0abili0as ;arna-an.

/ihasil'an /ari &i.%en 2i'osianin sehin..a san.a0 oo' /i.una'an /ala% in/us0ri

&an.an se&er0i on2e0ioner- /an &as0r-! Rea'si an0ara 'onsen0rasi .ula -an. 0in..i

/en.an 2i'osianin 8u.a /a&a0 %e%&er&an8an. u%ur si%&an ser0a %en.ha%ba0

'erusa'an 4Mar0elli et al., +#1arra hEoha 1"$6! Ro%a- et al. 416 %ena%bah'an bah;a &a/as0ur'0ur 2i'osianin %e%ili'i ran0ai tertraphyrroles 0erbu'a -an. %a%&u un0u'

%enan.'a& ra/i'al o'si.en! S0ru'0ur 'i%ia chromophores&a/a 2i'osianin %e%&un-a

'e%iri&ian /en.an bilirubin! ,ilirubin a/alah sen-a;a an0io'si/an -an. &en0in. un0u'

2isolo.is 'arena sen-a;a an0io'si/an /a&a0 %en.i'a0 ra/i'al bebas /en.an

%en/onor'an a0on hi/ro.en &a/a a0o% >1# &a/a %ole'ul tetraphyrroles. Fi'osianin

8u.a %eru&a'an salah sa0u /ari 0i.a &ii.%en selain 'loro2il /an 'aro0enoi/ -an.

/i.una'an un0u' %enan.'a& ra/iasi %a0ahari &alin. e2isien /iban/in. &i.%en lain 4Hall Rao 16!

3.3. Dekstri

De's0rin %eru&a'an &olisa'ari/a /ari &roses hi/rolisa &a0i -an. /ia0ur oleh eni%

0er0en0u /en.an ara &en.hi/rolisis /en.an asa%! @arna /ari /e's0rin a/alah &u0ih

hin..a 'unin. %u/ah laru0 air lebih e&a0 0er/is&ersi 0i/a' 'en0a' lebih sa0bil

/iban/in. /en.an &a0i 4Re-nol/ 1+6! Selain i0u Ribua0 /an Ku%alanin.sih 4+##


11/20

%ena%bah'an /e's0rin 8u.a seba.ai a.en &e%ba;a bahan &an.an -an. a'0i2 on0h-a

bahan flavor /an &e;arna -an. %e%erlu'an si2a0 -an. %u/ah laru0 air /an bahan

&en.isi 'arena /e's0rin /a&a0 %enin.'a0'an bera0 &ro/u' bila /ala% ben0u' bubu'!

S0ru'0ur -an. /i%ili'i /e's0rin -ai0u s&iral sehin..a %a%&u un0u' %enan.'a& %ole'ul

flavor 4Arie2 1"6! Na%un 'ele%ahan /ari &ena%bahan /e's0rin a/alah /a&a0

%en.uran.i 8u%lah 'o%&onen -an. %u/ah %en.ua& sela%a &roses &en.olahan!

Na%un /e's0rin /aoa0 %elin/un.i s0abili0as flavor /ari &roses &en.erin.an /en.an

spray dryer /en.an suhu &anas -an. 0in..i /ala% &roses &e%bua0an bubu' &e;arna

4Su&ar0i +###6! Fa'0or -an. /i&erha0i'an /ala% &e%bua0an ;arna ala%i 2i'osianin

a/alah s0abili0as /ala% &roses &en-i%&anan! Mishra et al. 4+##6 ;arna 2i'osianin

%en8a/i &u/ar sebesar #? se0elah &en-i%&anan ( hari /an %en8a/i benin. se0elah 1(hari &a/a suhu (=>!

3.!. "ara Ker#a

Lan.'ah &er0a%a /ala% &e%bua0an &i.%en 2i'osianin /ari %i'roal.a a/alah bio%assa

s&irulina -an. berben0u' bubu' /i%asu''an /ala% erlen%e-er /an /ilaru0'an /en.an

a'ua/es /en.an &erban/in.an 1:1# &a/a s&irulina bubu' seban-a' . /an a'ua/es

seban-a' # %l! Menuru0 ,oussiba /an Rih%on/ 41#6 bio%assa spirulina sp%u/ah laru0 &a/a &elaru0 &olar se&er0i air /an laru0an bu22er /iban/in.'an /en.an

&elaru non &olar! ,an-a'n-a 'a/ar 2i'osianin -an. 0er'an/un. ber/asar'an su&lai

ni0ro.en -an. /i'onsu%si olehspirulina sp.

Hasil a%&uran /ila'u'an &en.a/u'an /en.an %en..una'an stirrer sela%a 'uran.

lebih + 8a%! Tu8uan /ari &en.a/u'an a/alah a.ar &ena%&uranpirulina /en.an a'ua/es

/a&a0 0era%&ur seluruhn-a sehin..a &roses e's0ra'si /a&a0 0erben0u' seara o&0i%al/an sela%a &roses &en.a/u'an a.ar hasiln-a o&0i%al %a'a /i0u0u& /en.an 'ain!

Menuru0 ,ela- an/ Gersh;in 4+##"6 2a'0or -an. ber&en.aruh 0erha/a& berhasiln-a

e's0ra'si a/alah a/a a0au 0i/a'n-a aha-a! A/an-a aha-a 'e0i'a &roses e's0ra'si a'an

0er8a/i 'enai'an suhu sehin..aspirulina sp.a'an %a0i! Te%&era0ur -an. o&0i%al /ala%

&er0u%buhan Spirulina sp. a/alah (=>=>!


12/20

Selan8un-a a%&uran /isen0ri2u.asi (### r&% sela%a 1# %eni0 sehin..a /i&eroleh

en/a&an /an su&erna0an 4airan berisi 2i'osianin6! Proses sen0ri2u.asi ber0u8uan un0u'

%en.en/a&'an /ebris sel /an %en.a%bil &i.%en 2i'osianin -an. 0erlaru0 &a/a a'ua/es

sehin..a su&erna0an -an. /i&eroleh %en.an/un. 2i'osisanin -an. 0eralur /ari &roses

sebelu%n-a 4Sil5eira et al., +##"6! Po%eran Meloan 41"6 %ena%ah'an bah;a

/ebris sel -an. %en.en/a& a'an %e%ban0u &roses &en.u'uran absorbansi sehin..a

0i/a' 0er.an..u 'arena laru0an -an. 0erlalu &e'a0 %en-ebab'an 'esalahan &e%baaan

/ari s&e'0ro2o0o%e0er! Prinsi& u0a%a /ala% sen0ri2u.asi a/alah &e%isahan subs0ansi

ber/asar'an bera0 8enis %ole'ul /en.an %e%beri'an .a-a sen0ri2u.al sehin..a

subs0ansi -an. lebih ber0a a'an bera/a /i/asar se/an.'an -an. lebih rin.an 0erle0a'

/ia0asn-a 4Ki%ball 1+6!

Ke%u/ian laru0an /iba.i /ua -ai0u seban-a' 1# %l /ila'u'an &en.eneran hin..a 1#+

/an /i u'ur 'a/ar 2i'osianinn-a /en.an /en.an s&e'0ro2o0o%e0er &an8an. .elo%ban.

$(+ n% se/an.'an sisan-a /iu'ur absorbansin-a /ne.an s&e'0ro2o0o%e0er &an8an.

.elo%ban. $1( n%! Pen.u'uran &an8an. .elo%ban. ber0u8uan un0u' %en.e0ahui

'elaru0an 2i'osianin &a/a laru0an 4Ah%a/i et al., 1+6! Menuru0 Sara/a et al. 416

'onsen0rasi 2i'osianin &a/a su&erna0an /i'e0ahui /ne.an &en.u'uran s&e'0ro2o0o%e0er/en.an &an8an. .elo%ban. $1( n% /an $(+ n%! ,eri'u0 a/alah &en.u'uran 'onsen0rasi

2i'osianin /isi%bol'an /en.an KF:

Konsen0rasi 2i'osianin 4KF6 9OD6150,474 (OD652)

5,34

Ke%u/ian /i0a%bah'an /e's0rin /en.an &erban/in.an 1:1! Pena%bahan /e'0rin

ber0u8uan un0u' %e%eran.'a& 2i'osianin sehin..a &roses &erusa'an &a/a &i.%ena'iba0 &e%u/aran ;arna /a&a0 /iha%ba0 4Tho%son +#116! Su&ar0i 4+###6 8u.a

%ena%bah'an sela%a &roses &en.erin.an &i.%en 2i'osianin /a&a0 %en8a/i lebih s0abil

se0elah /i0a%bah'an /e's0rin 'arena &a/a /asarn-a /e's0rin s0abil 0erha/a& &e%anasan!

Pena%bahan /e's0rin 8u.a &en0in. a.ar -iel/ -an. /ihasil'an lebih ban-a' /an bera0n-a

ber0a%bah 4Fenne%a 1(6! Se0elah 0era%&ur ra0a 'e%u/ian /i0uan.'an 'e/ala%

;a/ah -an. /a&a0 /i.una'an seba.ai alas un0u' &roses &en.erin.an! Lalu /i%asu''an

/ala% o5en suhu (#=> hin..a 'erin. hin..a 'a/ar air n-a se'i0ar "?! Se0elah


13/20

/i'erin.'an %a'a a'an %e%ben0u' a/onan 'erin. -an. .e%&al %a'a &erlu

i/hanur'an /en.an ala0 &enu%bu' hin..a %en8a/i &o;/er! Menuru0 Suhar0ono 4+###6

2i'osianin -an. berben0u' airan /a&a0 %en.ala%i &roses 2er%en0asi sehin..a u%ur

si%&ann-a 0i/a' la%a sehin..a &i.%en 2i'osianin biasan-a /isi%&an /ala% ben0u'

bubu' a.ar 0ahan la%a!

3.$. Hasi% Pe&a'ata

Dari &e%bua0an &i.%en 2i'oasianin /a&a0 /iliha0 bah;a &erla'uan se%ua 'elo%&o'

0i/a' a/a &e%be/aan sehin..a 0i/a' a/a &ara%e0er 'husus -an. /ia%a0i /ala%

&erobaan ini! Hasil /ari &en.u'uran absorbansi &a/a se0ia& sa%&el /ihasil'an OD$1(

/an OD$(+ -an. berbe/a 0ia& 'elo%&o'! Hasil absorbansi -an. berbe/a %en-ebab'an&erhi0un.an KF /an -iel/ -an. berbe/a! Da&a0 /iliha0 /ari nilai OD $1( -an. /i/a&a0'an

ber'isar (


14/20

0i/a' 0er8a/i &erubahan ;arna seara si.ni2i'an! Alasan lain -an. %en-ebab'an 0i/a'

a/an-a &erubahan ;arna sebelu% /an sesu/ah /io5en &a/a serbu' 2i'osianin a/alah

'onsen0rasi 'a/ar 2i'osianin a/a spirulina sp. lebih besar /iban/in. 'onsen0rasi

/e's0rin! Hal ini sesuai /en.an 0eori -an. /iun.'a&'an oleh Tho%son 4+#116

&ena%bahan /e's0rin &a/a bubu' 2i'osianin /a&a0 %en.ha%ba0 &e%u/aran ;arna -an.

/ia'iba0'an /ari &e%anasan3&en.erin.an sehin..a hasil ;arna /ari sebelu% /io5en /an

sesu/ah /io5en sa%a! A/an-a &erbe/aan hasil &en.a%a0an &a/a 'elo%&o' A&h-o-anin 2ro%Spirulina plantesis in >on5en0ional an/ In0e.ra0e/ ABueous T;oPhase S-s0e%s!*!,ra>he% )ol!+1 No!(+1+$! ,rail!

Arie2 M! 41"6! Il%u Merai' Oba0 ,er/asar Teori Dan Pra'0e'! Uni5ersi0as

Ga8ah%a/a Press!Co.-a'ar0a!

,ela- A%ha an/ M! E! Gersh;in! 4+##"6! S&irulina in Hu%an Nu0ri0ion an/ Heal0h!>R> Press!

,oussiba S an/ Rih%on/ A! 41#6! Ph-oianin as a s0ora.e &ro0ein in 0he blue.reen al.a S&irulina &lan0esis! Arhi5es o2 Mirobiolo.- 1+( 1


17/20

Mishra SK Shri5as0a5 A /an Mishra S! 4+##6!(ffect of preservatives for food )radeCPC from Spirulina platensis! Proess ,iohe%is0r- -ano&h-eae6 isola0e/ 2ro% A2rian;a0ers! Toion he%is0r- an/ ,iohe%is0r- o2 Plan0 Pi.%en0s! Aa/e%i &ress in!Lon/on!

Pa%un.'as Es0ia%boro! 4+##(6! Pen.olahan Li%bah >air PT! Pu&u' Ku8an. /en.anSpirulina s&! &a/a Rea'0or >urah 4,a0h6! S'ri&si! ,o.or: De&ar0e%enTe'nolo.i Hasil Perairan Fa'ul0as Peri'anan /an Il%u Kelau0an IP,!

Po%eran C! >! E Meloan! 41"6! Foo/ Anal-sis Theor-lan/ Pra0ie! An A)I,oo'! Ne; Cor'!

Re-nol/s *a%es E!F! 41+6! Mar0in/ale The E0ra Phar%ao&olia E/i0ion T;en0-Ei.0h! The Phar%aen0ial Press! Lon/on!

Ribua0 S! /an S! Ku%alanin.sih 4+##


18/20

Shar%a Gaura5 et al. 4+#1arbon >on0en0 Salini0- an/ &H on Spirulinaplatensis 2or Ph-o-anin Allo&h-o-anin an/ Ph-oer-0hrin Au%ula0ion!*ournal ,iohe%ihal Tehnolo.-! In/ia!

Sil5eira S! T! ,ur'er0 *! F! M! >os0a *! A! )! ,ur'er0 >! A!)! Kalil S! *! ,ioresour!Tehnol! +##" 1$+!

Si5asan'ari et al. 4+#1o%&arison o2 Di22eren0 E0ra0ion Me0ho/s 2or Ph-o-aninE0ra0ion an/ Ciel/ 2ro% Spirulina platensis. In0erna0ional *ournal o2 >urren0Mirobiolo.- an/ A&&lie/ Sienes! In/ia!

S&olaore P *oanis>arson > Duran E /an Isa%ber0 A! 4+##$6! Commercial applicationof microal)ae! *ournal o2 biosiene an/ bioen.inerin. 1#14+6:"$!

Suhartono TS! 4#///$! ,io0e'nolo.i Hasil Lau0! ,o.or: Pusa0! Ka8ian Su%ber Da-aPesisir /an Lau0an! Ins0i0u0 Per0anian ,o.or!

Su&ar0i @! 4+###6! Pe%bua0an Pe;arna ,ubu' /ari E's0ra' An.'a': Pen.aruh SuhuTe'anan /an Konsen0rasi De's0rin! Tesis! Pro.ra% Pasasar8ana! Uni5ersi0as,ra;i8aa-a! Malan.!

Su&ar0i @! 4+###6! Pe%bua0an Pe;arna ,ubu' /ari E's0ra' An.'a': &en.aruh SuhuTe'anan /an Konsen0rasi De's0rin! Tesis!Pro.ra% Pasasar8ana! Uni5ersi0as

,ra;i8aa-a! Malan.!

Tho%&son >aroline! 4+#116! @ha0 Is @hea0 De0rinVh00&:33;;;!li5es0ron.!o%3ar0ile3


19/20

). LAMPIRAN

).1 Per*it+&a

KF4%.3%l6 9

OD

OD615

0,474 ( 652)5,34

1

Fp

Ciel/ 4%.3.6 9KFVol(total filtrat)g (berat Biomassa)

Kelo%&o' A1

KF4%.3%l6 90,0544 0,474 (0,0225)

5,34

1

102

9 #1%.3%l

Ciel/ 4%.3.6 90,81958

8

9 ( %.3.

Kelo%&o' A+

KF4%.3%l6 90,0569 0,474(0,0223)

5,34

1

102

9 #$%.3%l

Ciel/ 4%.3.6 90,86858

8

9 $+ %.3.

Kelo%&o' A

KF4%.3%l6 90,0568 0,474(0,0227)

5,34

1

102

9 #$+%.3%l


20/20

Ciel/ 4%.3.6 90,86258

8

9 $+(# %.3.

Kelo%&o' A

fabiana tara dewi_13.70.0166_kloter a_unika soegijapranata

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