brine shrimps lethality test
DESCRIPTION
laporan praktikum farmakologiTRANSCRIPT
7/17/2019 Brine Shrimps Lethality Test
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LAPORAN PRAKTIKUM FARMAKOLOGI II
BRINE SHRIMPS LETHALITY TEST (BSLT)
OLEH :
SUCI ANGRIANI (1301096 )
KELOMPOK 6
TANGGAL PRAKTIKUM
29 MEI 2015
DOSEN :
D! S"#$%! H!&'% M$!A*'
ASISTEN :
IS+AN PERMADI
RESTU ADITYA
PROGRAM STUDI SI FARMASI
SEKOLAH TINGGI ILMU FARMASI RIAU
PEKANBARU
2015
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BRINE SHRIMPS LETHALITY TEST (BSLT)
I. TUJUAN1, M-!.!% /! '-!*%# -#!! % '4&%&%'!& !' /-! -'4/- BSLT
(B%- S.%*& L-'.!#%'" T-&'),
2, T-!*%# /!#! --'!
%#!% LC 50 #!! A'-%! &!#%! L-!7. /-!
-'4/- BSLT (B%- S.%*& L-'.!#%'" T-&'),
II. TINJAUAN PUSTAKA
S-7!! '!/%&%4!# 8-8-!*! '8.! '-#!. /%!! ' -48!'% *-"!%'
!- !* *-"!%' %$-&%, B-8-!*! /!% '8.! '-&-8' -*"!% *4'-&%' /%-8!! -!/% &-/%!! $%'4$!!! /! &-8!!% &8- &-"!!
!'%!- !'%4&%/! !'%8!'-% /! !'%$!# "! 8!, P-7!%! &8- 48!'
/!% !#! &!!' -%! /% I/4-&%! "! !"! !! 8-8!!% &8- $#4! /!
$!!, P-!!%! 8!.! "! 8-&8- /!% !#! %% -#!& !! -%#%% -&%4
'4&%&%'!& "! #-8%. %! &-'! '%!' -!!! "! #-8%. '%% /%8!/%!
/-! 48!' &%'-&%& "! 8-!&!# /!% 8!.! %%! %,
K!- !/!#!. &!' *-"!%' "! '-!/% !%8!' *-'8.! '%/! 4!# /!%
&-#&-# !%! '8. &-#&-# "! '%/! 4!# %% (&-# !-) /!*!' -"-8! -
8!%! '8. #!%"! &-.%! /!*!' -"-8!8! -!'%!, U*!"! -8-8!&!
!&"!!!' /!% *-/-%'!! !- &!!' *-# /%#!! '-'!*% /%'-% 8!"!
-/!#! !-! 8%!"! *-48!'! *-"!%' !- "! !.!# &-'! 48!' !'%!-
-%#%% -$- &!*% "! 8-&!,
E$- &!*% '%8# !-! 8-# !/!"! 48!' !- "! 8--! &*-&%$%,
O8!' !'%!- "! !/! &-!! %% &-#!% 8--! *!/! &-# !- ! 8--! *!/!
&-#&-# "! -%#%% *-'8.! "! 7-*!' &-*-'% &-#&-# -#!% !8' /!
&-8!!%"!, O#-. &-8!8 %' &!.! ' --! 48!' !'%!- '-'!! "!
8--! &*-&%$% '-& /%#!! '-'!! "! 8-!&!# /!% 8!.! !#! (A4%2006),
S!#!. &!' % *-/!.#! "! /!*!' /%#!! !/!#!. /-! -'4/! Brine Shrimp
Lethality Test ( BSLT ) /-! -!! #!! /! #!' Artemia salina L-!7.,
M-'4/! %% -*!! *-!*%&! !!# /!#! *!"! *-7!%! &-"!! !'%!-
!-! .!&%# /!% % '4&%&%'!&"! -%#%% 4-#!&% *4&%'%$ /-! !'%%'!& &%'4'4&%
!'%!-,
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B%- S.%* L-'.!#%'" T-&' (BSLT) -*!! &!#!. &!' -'4/- '
-% 8!.!8!.! "! 8-&%$!' &%'4'4&%, M-'4/- %% -!! #!! A'-%!
&!#%! L-!7. &-8!!% .-! 748!, U% '4&%&%'!& /-! -'4/- BSLT %% -*!! %
'4&%&%'!& !' /%!! -$- '4&% /!% &!' &-"!! /%'-'! /!#! !' &%!'
&-'-#!. *-8-%! /4&%& %,
P4&-/"! /-! --'! %#!% LC 50 /!% !'%%'!& 4*4- !'%$
'!!! '-.!/!* #!! Artemia salina Leach. S!' -&'! /%!'!! !'%$ &-8!!%
!'%!- 8-/!&!! -'4/- BST %! .!! LC ; 1000 <= #, P--#%'%! C!8!##4
/ -! !/!"! .8! "! 4&%&'- !'!! &%'4'4&%&%'!& /! #-'!#%'!&
Brine shrimp *!/! -&'! '!!!, M-'4/- BSLT /!*!' /%*-7!"! ' -%
!'%%'!& '4&%4#4% /!% 8!.!8!.! !#!%,
M-'4/- %% /!*!' /%#!! /-! 7-*!' !. /!. /! 7*
-*4/&%8-# &-.%! /!*!' /%!! &-8!!% 8%4!&&!" G%/-/ I&4#!'%4 "!%' %&4#!&%
4*4- %%! 8-/!&!! !'%$%'!& "! /%'! 4#-. 8%4-&&!" '-&-8', D-!
--'!.% !'%$%'!& /!% &!' -#4*4 4*4- %%! ($!&%) /!*!' /%#!!
%&4#!&% &-"!! &-.%! /%*-4#-. &-"!! '!# !'%$,
T4&%&%'!& !/!#!. -$- 8-8!.!"! /!% &!' 8!.! 48!' *!/! 4! '!-', U%
'4&%&%'!& /%#!! ' --'!.% '%!' -!!! >!' "! !! /% %, A/!*
&8- >!' '4&% /!*!' 8-!&!# /!% 8!.! !#! !* &%'-&%& ,
T4&%&%'!& /% /-! -!!'% -!'%! *!/! .-! 748!, K-!'%!
.-! 748! /%!!* &-8!!% -&*4 /-! -!! -!'%! &-8!!% !!8!
'4&% !/!#!. '%'% !!# ' -*-#!!% '4&%&%'!&,
M-/%! L-'.!# D4&%& (LD50) !/!#!. /4&%& /!% &!*#- "! /%% "!
-!'%! 50? /!% .-! 748! &-/!! M-/%! L-'.!# C47-'!'%4 LC 50 !/!#!.
4&-'!&% &!*#- "! /%% "! /!*!' -!'%! 50? /!% .-! 748!, A!-!'%! /!% .-! *-748!! /%.%' &-8!!% M-/%! L-'.!# D4&%& (LD50) !'!
-/%! L-'!# C47-'!'%4 (LC50), P-!! LC50 /%!&/! ' *-%!
-'4&%! /-! *-#!! '-.!/!* .-! 748! &-7!! %.!#!&% !'! /-! -/%!
!%,
A'-%! &!#%! L-!7. -*!! 4*4- /!% %-'-8!'! /!% $!! *!/!
-4&%&'- *-!%! #!', U/! -% %% -*"!% *-!! "! *-'% /!#! !#%!
--% /! !'!% !!!, S*-&%-& %-'-8!'! %% "! /%!! &-8!!%
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4!%&- &-'%-# &-!'% 8-/!&!! *!/! *-"-8!! $!&%#%'!& &!*#% /! #!&"!
!!'-%&'% -4#4% /! &-&%$%'!&"! '-.!/!* 8!.! %%!,
P-%! L-'.!#%'!& '-#!. /%!! /-! &&-& ' %&4#!&% 8%44%'4
/!% 7"'4'4@%7!'%!#!%! %&-'%&%/! /! !'%$--/-' 7!*! /!% -'! '8.!,
H!&%# /!% &-% /!% !% ."/4!#74.4#%7 /! -&'! !#4.4# /!% 8-8-!*! '8.!
48!' *-'% "! /%!! /!#! *-48!'! '!/%&%4!# ' #-'.!#%'!& -
*!/! #!! A'-%! &!#%! "! /%*--!#!,
S!' 4&-'!&% -!'%! (L-'.!# C47-'!'%4) !/!#!. !!#%&! &-7!!
&'!'%&'% "! -!! % +.4#- E$$#-' T4@%7%'" (+ET) ' -!&%
#-'.!#%'!& &!*-# -$$#-, T-&' !' /%!! /% +%&74&% ' -!&% 4/%&%
!.% /!% *%*! ("!%' -$$#-' "! '%/!
/%#-!.! &-8!!% !/!"! /%8-8!&! *!/! #%!), K4&-'!&% -$$#- /%!!
50? /!% 4!%&- !'% &-#!! '-&' (LC50) /%!! &-8!!% *--.! '%'% !.%
(-/*4%') ' T-&' +.4#- E$$#-' T4@%7%'" (+ET) !',
D!#! !! -!##!&% LC50 &!#!. &!' /!% 4&-'!&% '-&' .!&
-"-8!8! 50? -!'%!, LC50 "! #-8%. -/!. 8-!'% &-!% 8-!7
-$$#-' '-&-8', S-8!!% 74'4. LC50 100? 8-!'% -!'! *-. -$$#-'
'-&-8' '%/! -8. #-8%. /!% &-*!. 4!%&-, LC50 &!! /-! 50? 8-!'%
&-*!. -$$#-' -*"!% -!'! -8. 50? /!% 4!%&- '-&-8',
U% '4&%&%'!& /%!&/! ' -!*!! !/!"! -$- '4&% /! !'!
-%#!% 8!'!& -!!! /!#! !%'!"! /-! *-!! &!' &-"!!,
P-! '4&%&%'!& /!*!' /%'-'! &-7!! !'%'!'%$ "! -"!'!! '%!'
-!!! /! '%!' 8-8!.!"! >!' '-&-8',
Penggunaan Artemia salina Lea! "alam #enelitian
S!' -'4/- % .!"!'% "! '-*!' /! !. ' &%% /!#! --'!'4&%&%'!& &!' -&'! '!!! !'%$ /-! -!! .-! % A'-%! &!#%!
L-!7., A'-%! &-8-#"! '-#!. /%!! /!#! 8-!7!!7! % .!"!'% &-*-'%
% *-&'%&%/! *4#'! %4'4&% !-&'-'% 4*4- &-*-'% 4$%
-!&%4-%! /! '4&%! /!#! !% #!',
U% /-! 4!%&- %% &-&!% ' !'%$%'!& $!!4#4% /!#! -&'!
'!!! "! 8-&%$!' '4&%, P--#%'%! -!! A'-%! &!#%! -%#%%
8-8-!*! -'! !'!! #!% 7-*!' /!. !. /! &-/-.!!, P--#%'%! /-!
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#!! A'-%! &!#%! L-!7. '-#!. /%!! 4#-. P&!' K!- P/- U%-&%'!&
P/- /% L!$!"-''- ' &-"!! !'%$ '!!! &-7!! /! '%/! &*-&%$% '
>!' !'% !-, N! /-%%! .8! "! &%%$%! /!% &!*-# "! 8-&%$!'
'4&% '-.!/!* #!! A'-%! &!#%! L-!7. '-"!'! ! -*"!% !'%$%'!& &%'4'4&%,
B-/!&!! .!# '-&-8' !! #!! A'-%! &!#%! L-!7. /!*!' /%!! ' %
'4&%&%'!&,
III. ALAT $AN BAHAN
Alat
% H!%/"
% %!#% P%*-' T-'-&
Ba!an
E&'!
M-'!4#
L!! U/! A'-%! &!#%! L-!7.
A% L!'
DMSO (D%-'%# S#$4&%/!)
I&. PR'SE$UR KERJA
. A8%# 05 # /!% #!'! %/ (-&'! ) "! '-#!. /%8!'
&-8-#"!!&! /!#! %!#,
. K-/%! 7*! /-! -'.!4# !/ 5 #8%!! -'.!4# -!*
/-! -!! .!%/" !! 7-*!',
*. S-'-#!. -'.!4# -!* '!8!.! 50 DMSO (D%-'%# &#$4&%/!)#!#
'!8!.! !% #!' 8-8-!*! '-'-&,
+. K-/%! !&! #!! /! A'-%! &!#%! /!#! %!# &-8!"! 10 -4!/
5 # /-! !% #!' ,
,. A!'% #!. -!'%! #!! /! &-#!!
&. HASIL
!, D!'! P-!!'!
K-
#
K4&-'!
&% L!'!
I/
K4&-'!&%
#!'!
&!*-#
#!.
#!!
#!.
#!!
!'%
?
K-!'%!
R!'!!'!
-!'%!
N%#!%
*48%'
1 10,000 1000 =# 10 9 90 ? 33 ? 55
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=# 0 ?
0 ?
2 10,000
=#
1000 =# 10 9 90? 66 ? 615
9 90 ?
0 ?3 1000
=#
100 =# 10 5 50 ? 333? 2
0 ?
0 ?
1000
=#
100 =# 10 0 ? 3666? 66
0 ?
3 30 ?
5 100 =# 10 =# 10 1 1 ? 133? 3
1 10 ?
2 20 ?
6 100 =# 10 =# 10 1 10 ? 66 ? 3521 10 ?
0 0 ?
-. PERHITUNAN
@ J L4 K4&-'!&%
" J N%#!% P48%'
• P-&!!! --&%:
" J 1325@ 213
5 J 1325 213
1325 @ J 5 213
@ J2,862
1,325 J 216
7/17/2019 Brine Shrimps Lethality Test
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• LC50
LC50 J ! #4 216
LC50 J 15=#
7, K!
0.5 1 1.5 2 2.5 3 3.5
0
1
2
3
4
5
6
7
f(x) = 1.33x + 2.14
Linear ()
&I. PEMBAHASAN
BSLT ( Brine Shrimp Lethality Test ) -*!! &!#!. &!' -'4/- &%%
8!.! "! 8-*4'-&% &-8!!% '!!! 8-.!&%!', M-'4/- *--#%'%! %% -!!
#!! /! (Artemia salina L-!7.,) &-8!!% 8%4%/%!'4, L!! /! %% -*!!
4!%&- &-/-.!! /!% 8%4'! #!' "! &!!' -7%# /! -*"!% -*-!! "!
7* '%% '-.!/!* '4&%, T-#"! -%#%% /!"! '!.! .%/* &-#!! 8-8-!*!
'!. /!#! -!/!! -%,
T-# /! /!#! !% #!' !! --'!& -!/% #!! (!*#%%) /!#! !' 2
2 !, B%#! 8!.! "! /%% -8-%! -$- '4&% '-.!/!* #!! /! !! .!#
%% -*!! %/%!&% !!# /!% -$- $!!4#4% "! '-!/ /!#! 8!.!
'-&-8', B-8-!*! *--#%'%! -! 8!.! A. salina -%#%% 4-#!&% *4&%'%$
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'-.!/!* -&'! "! 8-&%$!' 8%4!'%$, M-'4/! %% ! 8!"! /%!! /!#!
8-8!!% !!#%&%& 8%4&%&'% &-*-'% !!#%&%& '-.!/!* -&%/ *-&'%&%/! '4&% *4#&%
&-"!! '! 4$% /! !&%4-% /!% *.484# -&'-,
P!/! *-748!! !#% %% /%!! -'4/- BSLT ' % '4&%&%'!& 8!.!
'!!! 8! !'! -&'! "! 8-.!&%!' &-8!!% !'%!-, A!#"! &-8!"! 05
# /%!8%# /!% #!'! %/ "! '-#!. '-&-/%!/%!! 4&-'!&% '%!* -#4*4
8-8-/!8-/! #!% /!% 10010 /! 1 =#, S-'-#!. /%!8%# 05 #/%!&! /!#!
%!# -/%! /%'!8!.! -'.!4# &!*!% '!/! 8!'!& 5 # *!/! %!# /! 8%!!
-!* /-! -!! .!%/" !! #-8%. 7-*!', P-8!'! #!'! %
-!! *-#!' 4!% -'!4# !-! *-#!' %% /!*!' -#!'! .!*%
&-! &-"!! /! /!. -!* /! /%8%!! &!*!% -!* &-*! !!
'%/! -! *!/! *-%! '4&%&%'!& "! /%#!!,
K-/%! '!8!.! /%-'%# &#$4&%/! (DMSO) /! 8-8-!*! '-'-& !% #!',
P-8-%! DMSO 8-'! ' /!*!' -8!' -#!'! &-"!! % /!#! !%
#!' &-.%! &-"!! /!*!' '-/%&'%8&% &-7!! -!'!, B!"!"! DMSO "!
/%'!8!.! !/!#!. 50 <# !-! %! #-8%. /!% 50 <# !! /!*!' -"-8!8!
-!'%! *!/! #!! /! &%$!'"! "! '%/! '-#!# '4&% %% -!/% !#!&!
/%*%#%."! DMSO ' -8!' -#!'! &-"!! /!#! !% #!',
L!# !&! 10 -4 #!! /! !'-%! &!#%! '!8!.! !% #!' &!*!%
'!/! 8!'!& 5 # *!/! %!#, S-'-#!. %' *-!!'! /%#!! &-#!! 2 ! '
-#%.!' #!. #!! /! "! !'%, K-/%! /%.%' LC50 /-!
-8!/%! %#!% *48%' /-! #4 7 &!*-# "! /%*-4#-.,
P--#%'%! /-! #!! A'-%! &!#%! L-!7. '-#!. /%!! 4#-. P&!' K!-
P/- U%-&%'!& P/- /% L!$!"-''- ' &-"!! !'%$ '!!! &-7!! /!
'%/! &*-&%$% ' >!' !'% !-, N! /-%%! .8! "! &%%$%! /!%&!*-# "! 8-&%$!' '4&% '-.!/!* #!! A'-%! &!#%! L-!7. '-"!'! !
-*"!% !'%$%'!& &%'4'4&%, B-/!&!! .!# '-&-8' !! #!! A'-%! &!#%!
L-!7. /!*!' /%!! ' % '4&%&%'!&,
B-/!&!! .!# '-&-8' !! #!! A'-%! &!#%! L-!7. /!*!' /%!!
' % '4&%&%'!&, P!/! .!&%# "! /%*-4#-. '-#%.!' 8!.! LC50 #-8%. -7%# /!%
1000 =#, LC50 /!% -&'! &-8-&! 15 =# , P!/! -'4/! BSLT , &!*-# %
/%!'!! !'%$ %! LC50 -7%# /!% 1000 =#, !/% /!*!' /%&%*#! 8!.! -&'!
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"! '-#!. /%% /-! -!! -'4/- BSLT -%#%% -$- !'! 8-.!&%!'
&-8!!% 48!' !'%!-, LC50 J 15 =#,
&II. KESIMPULAN
B%- S.%* L-'.!#%'" T-&' (BSLT) -*!! &!#!. &!' -'4/- '
-% 8!.!8!.! "! 8-&%$!' &%'4'4&% '# 48!' !'%!-,
P%&%* /!% BSLT "!%' /-! --'! %#!% LC 50 /!% !'%%'!&
4*4- !'%$ '!!! '-.!/!* #!! Artemia salina leach.
S!' -&'! /%!'!! !'%$ &-8!!% !'%!- 8-/!&!! -'4/- BSLT %!
.!! LC 50 ; 1000 <= #,
S-!% -7%# .!! LC50 &-!% '4&% &!' &-"!!%% -!/!!
-&'! '-&-8' /!*!' /%-8! ' *-48!'! !'%!-,
E&'! "! '-#!. /%% '4&%&%'!&"! /-! -!! -'4/- BSLT
-%#%% *4'-&% '4&%&%'!& !' '-.!/!* #!! Artemia sal %! !'! -$- *4&%'%$
/%!! &-8!!% 48!' !'%!- /-! %#!% LC50 &-8-&! 15 =#,
$A/TAR PUSTAKA
P!!'% T, /! P, S%!'! 199, D!"! '4&% 8-8-!*! '8.! 48!' '!/%&%4!#
I/4-&%! !&!# N&! T-!! B!!', Journal Biologi Indonesia, 4# 11(3) : 11125,
Anderson, J.E and Mc. Laughlin, J.L., 1991. A Blind Comparison of Simple Bench
Top Bioassay and Human Tumour Cell Cytotoxicities as Antitumour Prescreens,
Phytochemical Analysis, Vol.2 .
Asakawa, Y., 1984. Phytocheistry o! "e#aticeae$ Isolation of Bioloically Acti!e
Aromatic Compound and Terpenoids, %e&. Latinoer. 'ui, Ja#an, hal.1(9)114
*ir+en PM, 19-9. arako#e /ndonesia Edisi ///. *e#kes %/. Jakarta.