brine shrimps lethality test

10
LAPORAN PRAKTIKUM FARMAKOLOGI II BRINE SHRIMPS LETHALITY TEST (BSLT) OLEH : SUCI ANGRIANI (1301096 ) KELOMPOK 6 TANGGAL PRAKTIKUM  29 MEI 2015 DOSEN : D! S"#$%! H!&'% M$!A*' ASISTEN : IS+A N PERMADI RESTU ADITYA PROGRAM STUDI SI FARMASI SEKOLAH TINGGI ILMU FARMASI RIAU PEKANBARU 2015

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7/17/2019 Brine Shrimps Lethality Test

http://slidepdf.com/reader/full/brine-shrimps-lethality-test 1/10

LAPORAN PRAKTIKUM FARMAKOLOGI II

BRINE SHRIMPS LETHALITY TEST (BSLT)

OLEH :

SUCI ANGRIANI (1301096 )

KELOMPOK 6

TANGGAL PRAKTIKUM

 29 MEI 2015

DOSEN :

D! S"#$%! H!&'% M$!A*'

ASISTEN :

IS+AN PERMADI

RESTU ADITYA

PROGRAM STUDI SI FARMASI

SEKOLAH TINGGI ILMU FARMASI RIAU

PEKANBARU

2015

7/17/2019 Brine Shrimps Lethality Test

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BRINE SHRIMPS LETHALITY TEST (BSLT)

I. TUJUAN1, M-!.!% /! '-!*%# -#!! % '4&%&%'!& !' /-! -'4/- BSLT

(B%- S.%*& L-'.!#%'" T-&'),

2, T-!*%# /!#! --'! 

%#!% LC 50 #!! A'-%! &!#%! L-!7. /-!

-'4/- BSLT (B%- S.%*& L-'.!#%'" T-&'),

II. TINJAUAN PUSTAKA

S-7!! '!/%&%4!# 8-8-!*! '8.! '-#!. /%!! ' -48!'% *-"!%'

!- !* *-"!%' %$-&%, B-8-!*! /!% '8.! '-&-8' -*"!% *4'-&%' /%-8!! -!/% &-/%!! $%'4$!!! /! &-8!!% &8- &-"!!

!'%!- !'%4&%/! !'%8!'-% /! !'%$!# "! 8!, P-7!%! &8- 48!'

/!% !#! &!!' -%! /% I/4-&%! "! !"! !! 8-8!!% &8- $#4! /!

$!!, P-!!%! 8!.! "! 8-&8- /!% !#! %% -#!& !! -%#%% -&%4

'4&%&%'!& "! #-8%. %! &-'! '%!' -!!! "! #-8%. '%% /%8!/%!

/-! 48!' &%'-&%& "! 8-!&!# /!% 8!.! %%! %,

K!- !/!#!. &!' *-"!%' "! '-!/% !%8!' *-'8.! '%/! 4!# /!%

&-#&-# !%! '8. &-#&-# "! '%/! 4!# %% (&-# !-) /!*!' -"-8! -

 8!%! '8. #!%"! &-.%! /!*!' -"-8!8! -!'%!, U*!"! -8-8!&!

!&"!!!' /!% *-/-%'!! !- &!!' *-# /%#!! '-'!*% /%'-% 8!"! 

-/!#! !-! 8%!"! *-48!'! *-"!%' !- "! !.!# &-'! 48!' !'%!- 

-%#%% -$- &!*% "! 8-&!,

E$- &!*% '%8# !-! 8-# !/!"! 48!' !- "! 8--! &*-&%$%,

O8!' !'%!- "! !/! &-!! %% &-#!% 8--! *!/! &-# !- ! 8--! *!/!

&-#&-# "! -%#%% *-'8.! "! 7-*!' &-*-'% &-#&-# -#!% !8' /!

&-8!!%"!, O#-. &-8!8 %' &!.! ' --! 48!' !'%!- '-'!! "!

 8--! &*-&%$% '-& /%#!! '-'!! "! 8-!&!# /!% 8!.! !#! (A4%2006),

S!#!. &!' % *-/!.#! "! /!*!' /%#!! !/!#!. /-! -'4/!  Brine Shrimp

 Lethality Test ( BSLT ) /-! -!! #!! /! #!'  Artemia salina L-!7.,

M-'4/! %% -*!! *-!*%&! !!# /!#! *!"! *-7!%! &-"!! !'%!- 

!-! .!&%# /!% % '4&%&%'!&"! -%#%% 4-#!&% *4&%'%$ /-! !'%%'!& &%'4'4&% 

!'%!-,

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B%- S.%* L-'.!#%'" T-&'  (BSLT) -*!! &!#!. &!' -'4/- ' 

-% 8!.!8!.! "! 8-&%$!' &%'4'4&%, M-'4/- %% -!! #!! A'-%!

&!#%! L-!7. &-8!!% .-! 748!, U% '4&%&%'!& /-! -'4/- BSLT %% -*!! %

'4&%&%'!& !' /%!! -$- '4&% /!% &!' &-"!! /%'-'! /!#! !' &%!'

&-'-#!. *-8-%! /4&%& %,

P4&-/"! /-! --'! %#!% LC 50 /!% !'%%'!& 4*4- !'%$ 

'!!! '-.!/!* #!!  Artemia salina Leach. S!' -&'! /%!'!! !'%$ &-8!!%

!'%!- 8-/!&!! -'4/- BST %! .!! LC ; 1000 <= #, P--#%'%! C!8!##4

/ -! !/!"! .8! "! 4&%&'- !'!! &%'4'4&%&%'!& /! #-'!#%'!&

 Brine shrimp  *!/! -&'! '!!!, M-'4/- BSLT /!*!' /%*-7!"! ' -%

!'%%'!& '4&%4#4% /!% 8!.!8!.! !#!%, 

M-'4/- %% /!*!' /%#!! /-! 7-*!' !. /!. /! 7*

-*4/&%8-# &-.%! /!*!' /%!! &-8!!% 8%4!&&!" G%/-/ I&4#!'%4 "!%' %&4#!&%

4*4- %%! 8-/!&!! !'%$%'!& "! /%'! 4#-. 8%4-&&!" '-&-8', D-!

--'!.% !'%$%'!& /!% &!' -#4*4 4*4- %%! ($!&%) /!*!' /%#!!

%&4#!&% &-"!! &-.%! /%*-4#-. &-"!! '!# !'%$,

T4&%&%'!& !/!#!. -$- 8-8!.!"! /!% &!' 8!.! 48!' *!/! 4! '!-', U%

'4&%&%'!& /%#!! ' --'!.% '%!' -!!! >!' "! !! /% %, A/!*

&8- >!' '4&% /!*!' 8-!&!# /!% 8!.! !#! !* &%'-&%& ,

T4&%&%'!& /% /-! -!!'% -!'%! *!/! .-! 748!, K-!'%!

.-! 748! /%!!* &-8!!% -&*4 /-! -!! -!'%! &-8!!% !!8!

'4&% !/!#!. '%'% !!# ' -*-#!!% '4&%&%'!&,

M-/%! L-'.!# D4&%& (LD50) !/!#!. /4&%& /!% &!*#- "! /%% "!

-!'%! 50? /!% .-! 748! &-/!! M-/%! L-'.!# C47-'!'%4 LC 50 !/!#!.

4&-'!&% &!*#- "! /%% "! /!*!' -!'%! 50? /!% .-! 748!, A!-!'%! /!% .-! *-748!! /%.%' &-8!!% M-/%! L-'.!# D4&%& (LD50) !'!

-/%! L-'!# C47-'!'%4 (LC50), P-!! LC50 /%!&/! ' *-%!

-'4&%! /-! *-#!! '-.!/!* .-! 748! &-7!! %.!#!&% !'! /-! -/%!

!%,

A'-%! &!#%! L-!7. -*!! 4*4- /!% %-'-8!'! /!% $!! *!/!

-4&%&'- *-!%! #!', U/! -% %% -*"!% *-!! "! *-'% /!#! !#%!

--% /! !'!% !!!, S*-&%-& %-'-8!'! %% "! /%!! &-8!!%

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4!%&- &-'%-# &-!'% 8-/!&!! *!/! *-"-8!! $!&%#%'!& &!*#% /! #!&"!

!!'-%&'% -4#4% /! &-&%$%'!&"! '-.!/!* 8!.! %%!,

P-%! L-'.!#%'!& '-#!. /%!! /-! &&-& ' %&4#!&% 8%44%'4 

/!% 7"'4'4@%7!'%!#!%! %&-'%&%/! /! !'%$--/-' 7!*! /!% -'! '8.!,

H!&%# /!% &-% /!% !% ."/4!#74.4#%7 /! -&'! !#4.4# /!% 8-8-!*! '8.!

48!' *-'% "! /%!! /!#! *-48!'! '!/%&%4!# ' #-'.!#%'!& - 

 *!/! #!! A'-%! &!#%! "! /%*--!#!,

S!' 4&-'!&% -!'%! (L-'.!# C47-'!'%4) !/!#!. !!#%&! &-7!!

&'!'%&'% "! -!! % +.4#- E$$#-' T4@%7%'" (+ET) ' -!&% 

#-'.!#%'!& &!*-# -$$#-, T-&' !' /%!! /% +%&74&% ' -!&% 4/%&%

!.% /!% *%*! ("!%' -$$#-' "! '%/!

/%#-!.! &-8!!% !/!"! /%8-8!&! *!/! #%!), K4&-'!&% -$$#- /%!!

50? /!% 4!%&- !'% &-#!! '-&' (LC50) /%!! &-8!!% *--.! '%'% !.% 

(-/*4%') ' T-&' +.4#- E$$#-' T4@%7%'" (+ET) !',

D!#! !! -!##!&% LC50 &!#!. &!' /!% 4&-'!&% '-&' .!&

-"-8!8! 50? -!'%!, LC50 "! #-8%. -/!. 8-!'% &-!% 8-!7

-$$#-' '-&-8', S-8!!% 74'4. LC50 100? 8-!'% -!'! *-. -$$#-'

'-&-8' '%/! -8. #-8%. /!% &-*!. 4!%&-, LC50 &!! /-! 50? 8-!'%

&-*!. -$$#-' -*"!% -!'! -8. 50? /!% 4!%&- '-&-8',

U% '4&%&%'!& /%!&/! ' -!*!! !/!"! -$- '4&% /! !'!

-%#!% 8!'!& -!!! /!#! !%'!"! /-! *-!! &!' &-"!!,

P-! '4&%&%'!& /!*!' /%'-'! &-7!! !'%'!'%$ "! -"!'!! '%!'

-!!! /! '%!' 8-8!.!"! >!' '-&-8',

Penggunaan Artemia salina Lea! "alam #enelitian

S!' -'4/- % .!"!'% "! '-*!' /! !. ' &%% /!#! --'!'4&%&%'!& &!' -&'! '!!! !'%$ /-! -!! .-! % A'-%! &!#%!

L-!7., A'-%! &-8-#"! '-#!. /%!! /!#! 8-!7!!7! % .!"!'% &-*-'%

% *-&'%&%/! *4#'! %4'4&% !-&'-'% 4*4- &-*-'% 4$%

-!&%4-%! /! '4&%! /!#! !% #!',

U% /-! 4!%&- %% &-&!% ' !'%$%'!& $!!4#4% /!#! -&'! 

'!!! "! 8-&%$!' '4&%, P--#%'%! -!! A'-%! &!#%! -%#%%

 8-8-!*! -'! !'!! #!% 7-*!' /!. !. /! &-/-.!!, P--#%'%! /-!

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#!! A'-%! &!#%! L-!7. '-#!. /%!! 4#-. P&!' K!- P/- U%-&%'!&

P/- /% L!$!"-''- ' &-"!! !'%$ '!!! &-7!! /! '%/! &*-&%$% ' 

>!' !'% !-, N! /-%%! .8! "! &%%$%! /!% &!*-# "! 8-&%$!'

'4&% '-.!/!* #!! A'-%! &!#%! L-!7. '-"!'! ! -*"!% !'%$%'!& &%'4'4&%,

B-/!&!! .!# '-&-8' !! #!! A'-%! &!#%! L-!7. /!*!' /%!! ' %

'4&%&%'!&,

III. ALAT $AN BAHAN

 

Alat

% H!%/"

% %!#% P%*-' T-'-&

 

Ba!an

E&'!

M-'!4#

L!! U/! A'-%! &!#%! L-!7.

A% L!'

DMSO (D%-'%# S#$4&%/!)

I&. PR'SE$UR KERJA

. A8%# 05 # /!% #!'! %/ (-&'! ) "! '-#!. /%8!'

&-8-#"!!&! /!#! %!#,

. K-/%! 7*! /-! -'.!4# !/ 5 #8%!! -'.!4# -!*

/-! -!! .!%/" !! 7-*!',

*. S-'-#!. -'.!4# -!* '!8!.! 50 DMSO (D%-'%# &#$4&%/!)#!#

'!8!.! !% #!' 8-8-!*! '-'-&,

+. K-/%! !&! #!! /! A'-%! &!#%! /!#! %!# &-8!"! 10 -4!/

5 # /-! !% #!' ,

,. A!'% #!. -!'%! #!! /! &-#!!

&. HASIL

!, D!'! P-!!'!

K-

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K4&-'!

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&!*-#

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K-!'%!

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1 10,000 1000 =# 10 9 90 ? 33 ? 55

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1325 @ J 5 213

  @ J2,862

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LC50 J ! #4 216

LC50 J 15=#

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0.5 1 1.5 2 2.5 3 3.5

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2

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f(x) = 1.33x + 2.14

 

Linear ()

&I. PEMBAHASAN

BSLT ( Brine Shrimp Lethality Test ) -*!! &!#!. &!' -'4/- &%%

 8!.! "! 8-*4'-&% &-8!!% '!!! 8-.!&%!', M-'4/- *--#%'%! %% -!!

#!! /! (Artemia salina L-!7.,) &-8!!% 8%4%/%!'4, L!! /! %% -*!!

4!%&- &-/-.!! /!% 8%4'! #!' "! &!!' -7%# /! -*"!% -*-!! "!

7* '%% '-.!/!* '4&%, T-#"! -%#%%  /!"! '!.! .%/* &-#!! 8-8-!*!

'!. /!#! -!/!! -%,

T-#  /! /!#! !% #!' !! --'!& -!/% #!! (!*#%%) /!#! !' 2

  2 !, B%#! 8!.! "! /%% -8-%! -$- '4&% '-.!/!* #!! /! !! .!#

%% -*!! %/%!&% !!# /!% -$- $!!4#4% "! '-!/ /!#! 8!.!

'-&-8', B-8-!*! *--#%'%! -! 8!.!  A. salina -%#%% 4-#!&% *4&%'%$ 

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'-.!/!* -&'! "! 8-&%$!' 8%4!'%$, M-'4/! %% ! 8!"! /%!! /!#!

 8-8!!% !!#%&%& 8%4&%&'% &-*-'% !!#%&%& '-.!/!* -&%/ *-&'%&%/! '4&% *4#&%

&-"!! '! 4$% /! !&%4-% /!% *.484# -&'-,

P!/! *-748!! !#% %% /%!! -'4/- BSLT ' % '4&%&%'!& 8!.!

'!!! 8! !'! -&'! "! 8-.!&%!' &-8!!% !'%!-, A!#"! &-8!"! 05

# /%!8%# /!% #!'! %/ "! '-#!. '-&-/%!/%!! 4&-'!&% '%!* -#4*4 

 8-8-/!8-/! #!% /!% 10010 /! 1 =#, S-'-#!. /%!8%# 05 #/%!&! /!#!

%!# -/%! /%'!8!.! -'.!4# &!*!% '!/! 8!'!& 5 # *!/! %!# /! 8%!!

-!* /-! -!! .!%/" !! #-8%. 7-*!', P-8!'! #!'! %

-!! *-#!' 4!% -'!4# !-! *-#!' %% /!*!' -#!'! .!*% 

&-! &-"!! /! /!. -!* /! /%8%!! &!*!% -!* &-*! !! 

'%/! -! *!/! *-%! '4&%&%'!& "! /%#!!,

K-/%! '!8!.! /%-'%# &#$4&%/! (DMSO) /! 8-8-!*! '-'-& !% #!',

P-8-%! DMSO 8-'! ' /!*!' -8!' -#!'! &-"!! % /!#! !% 

#!' &-.%! &-"!! /!*!' '-/%&'%8&% &-7!! -!'!, B!"!"! DMSO "!

/%'!8!.! !/!#!. 50 <# !-! %! #-8%. /!% 50 <# !! /!*!' -"-8!8!

-!'%! *!/! #!! /! &%$!'"! "! '%/! '-#!# '4&% %% -!/% !#!&!

/%*%#%."! DMSO ' -8!' -#!'! &-"!! /!#! !% #!',

L!# !&! 10 -4 #!! /! !'-%! &!#%! '!8!.! !% #!' &!*!%

'!/! 8!'!& 5 # *!/! %!#, S-'-#!. %' *-!!'! /%#!! &-#!! 2 ! ' 

-#%.!' #!. #!! /! "! !'%, K-/%! /%.%' LC50 /-!

-8!/%! %#!% *48%' /-! #4 7 &!*-# "! /%*-4#-.,

P--#%'%! /-! #!! A'-%! &!#%! L-!7. '-#!. /%!! 4#-. P&!' K!- 

P/- U%-&%'!& P/- /% L!$!"-''- ' &-"!! !'%$ '!!! &-7!! /!

'%/! &*-&%$% ' >!' !'% !-, N! /-%%! .8! "! &%%$%! /!%&!*-# "! 8-&%$!' '4&% '-.!/!* #!! A'-%! &!#%! L-!7. '-"!'! !

-*"!% !'%$%'!& &%'4'4&%, B-/!&!! .!# '-&-8' !! #!! A'-%! &!#%!

L-!7. /!*!' /%!! ' % '4&%&%'!&,

B-/!&!! .!# '-&-8' !! #!! A'-%! &!#%! L-!7. /!*!' /%!!

' % '4&%&%'!&, P!/! .!&%# "! /%*-4#-. '-#%.!' 8!.! LC50 #-8%. -7%# /!%

1000 =#, LC50 /!% -&'! &-8-&! 15 =# , P!/! -'4/! BSLT , &!*-# %

/%!'!! !'%$ %! LC50 -7%# /!% 1000 =#, !/% /!*!' /%&%*#! 8!.! -&'! 

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"! '-#!. /%% /-! -!! -'4/- BSLT -%#%% -$- !'! 8-.!&%!'

&-8!!% 48!' !'%!-, LC50 J 15 =#,

&II. KESIMPULAN

 

B%- S.%* L-'.!#%'" T-&'  (BSLT) -*!! &!#!. &!' -'4/- ' 

-% 8!.!8!.! "! 8-&%$!' &%'4'4&% '# 48!' !'%!-,

 

P%&%* /!% BSLT "!%' /-! --'! %#!% LC 50 /!% !'%%'!&

4*4- !'%$ '!!! '-.!/!* #!! Artemia salina leach.

 

S!' -&'! /%!'!! !'%$ &-8!!% !'%!- 8-/!&!! -'4/- BSLT %!

.!! LC 50 ; 1000 <= #, 

S-!% -7%# .!! LC50 &-!% '4&% &!' &-"!!%% -!/!!

-&'! '-&-8' /!*!' /%-8! ' *-48!'! !'%!-,

 

E&'! "! '-#!. /%% '4&%&%'!&"! /-! -!! -'4/- BSLT

-%#%% *4'-&% '4&%&%'!& !' '-.!/!* #!! Artemia sal %! !'! -$- *4&%'%$ 

/%!! &-8!!% 48!' !'%!- /-! %#!% LC50 &-8-&! 15 =#,

$A/TAR PUSTAKA

P!!'% T, /! P, S%!'! 199, D!"! '4&% 8-8-!*! '8.! 48!' '!/%&%4!#

I/4-&%! !&!# N&! T-!! B!!', Journal Biologi Indonesia, 4# 11(3) : 11125,

  Anderson, J.E and Mc. Laughlin, J.L., 1991. A Blind Comparison of Simple Bench

Top Bioassay and Human Tumour Cell Cytotoxicities as Antitumour Prescreens,

Phytochemical Analysis, Vol.2 .

 Asakawa, Y., 1984. Phytocheistry o! "e#aticeae$ Isolation of Bioloically Acti!e

 Aromatic Compound  and Terpenoids, %e&. Latinoer. 'ui, Ja#an, hal.1(9)114

*ir+en PM, 19-9. arako#e /ndonesia Edisi ///. *e#kes %/. Jakarta.

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0aniswarna, 199, "arma#oloi dan Terapi edisi IV , 2adan arakologi akultas

3edokteran ni&ersitas /ndonesia, Jakarta.