target (primer name) dbla1.5/6a cidra1.7 cidra3.4 …...target (primer name) dbla1.5/6a cidra1.7...

Target (primer name) DBLa1.5/6a

CIDRa1.7 CIDRa3.4 DBLa2/a1.1/2/4/7 CIDRa1.4 & CIDRa1 DBLa1.7 DBLg4/6 of DC8 DBLa1 not var3 DBLa0.9 CIDRd DBLa1.1 CIDRa1.6 DBLz5 CIDRa1.1 DBLa1.5/6b

DBLa0.1 DBLg of DC9 DBLb12 & DBLb3/5 DBLz4 DBLg of DC6 DBLb7&9 DBLe12 CIDRg2/9 CIDRa2.3/5/6/7/9/10 DBLa1.4 DBLa0.6/9 DBLg9 DBLb3 DBLb5 DBLa_CIDRa of DC8 DBLg of DC5 CIDRa1.4 DBLz6 DBLa0.16 DBLe2 DBLe8 DBLe3 CIDRg1/2 CIDRa2.2 CIDRg CIDRa3.1-3

Forward primer GATTAYGTCCCTCAATTTTTAMGWTGGT CGGAAACTATAACGTGGAAAGATAA GAACCTGAKSAATTCCAAAAGACRT TCCTACCAATTTAGATTACGTCCCTCA GGGTTAGAAAWTTMTTAATAGACACTA GATTAYGTCCCTCAATTTTTAMGWTGGT AGAAACAHATTTTGCKTGG TTGGGRAATBTGTTAGTTAYRGCAA TTTCTSAAGTAMRTGGCGGTGAATGTG TAAATGTAACTTASATGTATGTGAA TTGATGAAGATGAAGAATCTGAATG AGAAGGCGCAATAAAAGTGTTGT ATATTACCAAAGAAGGTGGATATAT TAARGTGGGAACATCAACTTAAGAAT ATTGTAGTMRKGAGGGTTACGATTGTA AGTAGAAWAGGTAAGGTGCGTA ATTTACACGAWSAATTAVAAAAACTTTGT CATCAAGTKTGGAGAGCTATGAAATGTG ATWVNGAACCTCCTGATTATGAT AGACAACCACAAACATCTGCTTGCAG AWGATTGGTGGGAAGCWAAYMGWG AATTACCTAAYGAAGATGAAATTCCTCA GATGGTTAAGATATTTTRTACASGATT TACARMATATTGYSAAGCATGTCCG GGTTTCATACTAATGTATGGCATCGTAA GAAGGACAATCAATAACACAAGATTATC CCSAATTTTTSMGATYCATGTT TGTRAAACAAATMGCACAATATTATAAA AACAYATGTGTACMTCNAATTTAGAA GYTCARAWTATTGCCAACCC GATTATGAKGGRTGTGAAAA AACAATGGAAATGCTATTAYRA AWWVNGAWCCMCCTGATTATGA ATATTTYGAYTAYGTGCCRC AAATTTAATAGGTTTGGRAGCACA AAGAGGATCTACTTAATGCTGCTTTTAG TGTATTGGTACCTCCTAGAAGA GATGAATTATGGGGATGTACTGRT ATGGTTCGATTATTGATATG SAWATGATTGATCGTAGGGGACC HWWMCAAAAGACATTCAATCC

Reverse primer GATTTGTTTTATTACAATCGTAACCCTC CTTTTTGTTTAACCCATYTGTCAAAAC CCTCTCCAATACATAGAATCGTTCAA CGTACAATCATATCCATTAAGACTAC CTTTTTGTTTAACCCATYTGTCAAAAC GTTTACGAAATGCTTCTCGTTGATTRCC CCATGTTCWTTCCACCAGTCDTCAAG CTGCAAAACTKCGWGCAAG AAATTTCTAACGCATAAATKTAATCG AATACTYTAACCAACGTTTAATMAATAC GAGCTTCCAAGTTTTTATCACACAT GAATTATTGTCTATGCATCTTTCAGC ATTCTCACAAWTTTTCATCGWTTCC CTAAATCTTYCGTAAATTGATCCCAT CWTYTKCACAAYKTGGACAWTCTARATC CTATCCAATTTTCATAACTATG CTTTAGGTGGATWATCSAAAGCTTCAGG TAATCTTCTATKGGGATACCATTACA ATTCACTCCATTCTTGCAAAAAACGA TTAGGMGGAYAATCGAACGCTTCAGG CAAGAGGGGTAACAGTGGTAGTATC CTTTACATGCTTGTTTTGCCCATTCAA TCRYCTTCAACCACMTYTTGGGC TTTATCACCGGTAAGTATTGGTAT GMACGACTAAAAAGACAAGGATTTCT CATAGTACATATTTGAGAAGGAGA RACMATATTCTTTCCACCAS GCATCRSCYTTCAAAGCWCTTCT TAGCGWTTTATTATCTCAKCTGCATC ACCSTYACAWWBAACTACAC KYGTCGTAGGTTTTATGAGG TCGKTTTCCCACTTTATASTGTC TTCACTCCATTCTTGCATCCAACG WKWTTCATACAKACGACACC ACCATAATCTGCATAWGAYCTTTTCAT AACTGAACTTCATAGCCTCATATGC TTAATCTTTTAGCTTCAGAAAATGCMGAT AATTAAATTTGTTATAAAATCKC CCATCGTTTATAACAWTCAC TAAATGTAGTATAKTSATTRAGGTCTA TGTTCTCCARTRTATGGAATC

Fragment length bp 157 117 85 201 138 109/121 103,109, 121 104 120 69 182 276 90 79 135 80 66 780 107 72 292 150 64 78,84 201 87 133 50 278 160 65 235,244 99 134 87 128 184-220 76

PCR sequencing (gDNA used) 3D7 3D7 3D7 3D7 3D7 3D7 3D7 3D7 3D7 3D7 3D7 3D7 3D7 3D7 3D7 3D7 3D7 3D7 3D7 3D7 3D7 3D7 3D7 3D7 3D7 3D7 3D7 3D7 3D7 3D7 3D7 3D7 HB3 3D7 3D7 3D7 3D7 3D7 3D7 3D7 3D7

Total read count 129 223 1456 527 587 129 457 1724 380 616 410 744 348 244 408 405 464 1028 1254 780 2250 930 471 684 3250 1064 353 1808 501 1004 123 1040 3221 151 122 101 PCR product not sequenced PCR product not sequenced PCR product not sequenced PCR product not sequenced PCR product not sequenced

Specificity (reads from intended domain type/total read count) 1,00 1,00 1,00 1,00 1,00 1,00 1,00 0,92 0,89 1,00 1,00 1,00 1,00 1,00 1,00 1,00 0,98 0,74 66,00 1,00 0,99 1,00 1,00 1,00 1,00 1,00 1,00 1,00 1,00 1,00 1,00 0,85 1,00 1,00 1,00 1,00

Coverage (amplified domains/domain type abundance in genome) 2/3 1/1 7/7 5/6 1/1 1/1 3/3 6/6 3/6 1/2 1/1 1/2 2/2 1/1 1/3 1/9 1/2 3/3 2/2 3/3 2/2 1/1 1/4 3/15 1/2 2/7 1/1 1/4 1/2 2/2 1/2 1/1 4/6 6/6 1/1 3/3

PCR amplicons graphic

Global specificity (# intended domains amplified/all amplified domains) 1,00 1,00 1,00 0,92 0,56 0,86 0,64 1,00 0,80 1,00 0,78 0,83 1,00 1,00 0,76 0,80 1,00 0,48 1,00 1,00 0,80 1,00 1,00 1,00 1,00 0,76 1,00 0,92 0,79 0,77 1,00 0,89 1,00 0,74 1,00 1,00 1,00 0,85 1,00 1,00 0,97

Global coverage (# domains amplified/domain type abundance) 17/26 8/8 17/19 44/64 9/12 6/9 7/11 67/76 16/24 21/22 7/12 5/7 8/9 9/9 19/26 4/39 11/12 10/12 11/12 10/11 4/12 4/6 12/19 34/47 5/12 13/37 4/9 11/29 11/30 10/12 8/12 8/10 15/15 13/16 11/12 7/8 13/14 17/39 9/15 51/100 99/107

PCR amplicons graphic

Figure S1. Var subtype Q-PCR primers and validation. Each primer is named by its PfEMP1 target. Primer targets are validated by FLX sequencing of 3D7, HB3 or FCR3 gDNA amplicons. The number of acquired sequence reads for each PCR is given, followed by the fraction of reads from the intended domain type ( i.e. the var typing specificity of the

primer pair) and the number of said domain types amplified by the PCR compared to number of said domain types in the tested genome (i.e. the var typing coverage of the primer pair). For all included primers actually observed amplicons (both signal and noise amplicons) were predicted bioinformatically. The domains predicted to be amplified from all known

annotated PfEMP1 (N=399, (13)) is given as fraction of domains of intended type vs. all domains amplified (i.e. “global specificity”) and amplified domains of said domain type vs. number of said domain type in the 399 PfEMP1 sequences (i.e. “global coverage”).

DBLa1.1

DBLa1.7

DBLa1.2

DBLa2

DBLa1.4

DBLa0.18

DBLa0.12

DBLa0.4

CIDRa1.4

CIDRa1.5

CIDRa1.8

CIDRa1.6

CIDRa1.1

CIDRa1.4

DBLa2

DBLa2

DBLa1.4

DBLa1.7

DBLa1.2

CIDRa3.4

CIDRa3.4

CIDRa3.4

CIDRa3.4

CIDRa3.4

CIDRa3.4

CIDRa3.4

CIDRa3.4

CIDRa3.4

DBLa1.5

DBLa1.6

DBLa1.5

CIDRa1.7

DBLa1.5

DBLa1.6 CIDRa1.7 CIDRa3.4

DBLa1.7

DBLa1.7

DBLa1.1

DBLg4

DBLg4

DBLg6

DBLg6

DBLg4

DBLg14

DBLa1.2

DBLa1.5

DBLa1.6

DBLa1.6

DBLa1.4

DBLa1.7

DBLa1.3

DBLa1.3

DBLa1.3

DBLa1.2

DBLa1.6

DBLa1.1

DBLa1.7

DBLa1.4

DBLa1.5

DBLa1.3

DBLa1.8

DBLa0.9

DBLa0.9

DBLα0.9

DBLα0.9

DBLa1.4

DBLa1.5

DBLa0.9

DBLa0.15

DBLa2

DBLa0.23

DBLb9

DBLb9

DBLb6

DBLb9

DBLb7

DBLb6

CIDRd

CIDRd

DBLa1.1

DBLa1.4

CIDRa1.6

CIDRa1.6

CIDRa1.4

DBLa1.1 DBLz5

DBLz5

DBLz5

CIDRa1.1

CIDRa1.1

DBLa1.5

DBLa1.5

DBLa1.6

DBLa1.7

DBLa1.4

DBLd

DBLa0.1

DBLa0.1

DBLa0.17

DBLg of DC9 DBLg16

DBLg of 9

DBLb12 DBLb12 DBLb12 DBLb3

DBLb12 DBLb3 DBLb5 DBLb6

DBLz4

DBLz4

DBLz4 DBLg of DC6

DBLg of DC6 DBLg of DC6 DBLg of DC6

DBLe12

DBLe12 CIDRg2

CIDRg9

CIDRg2

CIDRa2

CIDRa2

CIDRa2

CIDRa2

DBLa1.4

DBLa1.4

DBLa0.6

DBLa0.9

DBLa0.6

DBLa0.9

DBLa0.23

DBLa0.8

DBLg9

DBLg9

DBLb3

DBLb3

DBLb12

DBLb5

DBLb5

DBLb3

DBLb12

DBLa2_CIDRa1

DBLa2_CIDRa1

DBLa2_CIDRa1

DBLa1_CIDRa1

DC5 DBLg

DC5 DBLg CIDRa1.4

CIDRa1.8

DBLz6

DBLz6

DBLz6

DBLz6

DBLz6

DBLz6

DBLz6

DBLz6

DBLa0.16

DBLa0.16

DBLa0.16

DBLa0.16

DBLa0.16

DBLa0.16

DBLa0.7

DBLa0.8

DBLa0.24

DBLe2

DBLe2 DBLe3

CIDRg1

CIDRg2

CIDRg9

CIDRg7

CIDRa2.2 CIDRg

CIDRa3.1

CIDRa3.2

CIDRa3.3

CIDRa3.4

DBLe8

DBLe8

CIDRa1.4

CIDRa1.8

CIDRa1.6

Domain

DomainDBLα0.1

DBLα0.2

DBLα0.3

DBLα0.4

DBLα0.5

DBLα0.6

DBLα0.7

DBLα0.8

DBLα0.9

DBLα0.10

DBLα0.11

DBLα0.12

DBLα0.13

DBLα0.14

DBLα0.15

DBLα0.16

DBLα0.17

DBLα0.18

DBLα0.19

DBLα0.20

DBLα0.21

DBLα0.22

DBLα0.23

DBLα0.24

DBLα2

DBLα1.1

DBLα1.2

DBLα1.4

DBLα1.7

DBLα1.5

DBLα1.6

DBLα1.8

DBLα1.3

0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 # # # # # # # #

# Obs 40 3 11 12 22 13 7 16 24 9 11 11 8 4 15 13 8 13 6 6 6 5 5 3 13 12 13 12 9 12 11 6 6

%ID 57 71 63 58 59 60 62 55 58 64 59 58 64 63 57 60 61 55 59 60 73 58 58 62 57 71 61 60 61 63 59 62 79

Primer coverage

Primer coverage

Primer coverage

Domain

Domain

DBLδ1

DBLδ2

DBLδ3

DBLδ4

DBLδ5

DBLδ6

DBLδ7

DBLδ8

DBLδ9

DBLδ1

DBLδ2

DBLδ3

DBLδ4

DBLδ5

DBLδ6

DBLγ1

DBLγ2

DBLγ3

DBLγ4

DBLγ5

DBLγ6

DBLγ7

DBLγ8

DBLγ9

DBLγ10

DBLγ11

DBLγ12

DBLγ13

DBLγ14

DBLγ15

DBLγ16

DBLγ17

DBLγ18

9 # # # # # # 0 0 # # # # # 7 # # # # 0 46 ## # # 15 # # # # # # # #

# Obs ## 5 5 9 13 4 4 3 3 6 10 14 12 9 15 8 14 5 8 13 13 8 9 9 13 25 10 11 6 7 7 6 4

%ID 38 59 52 48 54 61 49 60 68 75 51 46 59 56 59 70 47 51 50 54 56 61 71 41 51 46 54 48 60 68 47 54 45

Primer coverage

Domain

Domain

DBLβ1

DBLβ10

DBLβ11

DBLβ12

DBLβ13

DBLβ2

DBLβ3

DBLβ4

DBLβ5

DBLβ6

DBLβ7

DBLβ8

DBLβ9

DB

Lpam

1

DB

Lpam

2

DB

Lpam

3

DBLεpam4

DBLεpam5

DBLε10

DBLε1

DBLε2

DBLε3

DBLε4

DBLε5

DBLε6

DBLε7

DBLε8

DBLε9

DBLε11

DBLε12

DBLε13

DBLε14

# 0 # # 0 0 # 0 0 # # 0 # 0 0 0 ## 0 14 # # 67 # # # # # # #

# Obs 10 6 7 12 4 7 31 5 30 12 12 10 5 11 11 10 10 10 10 11 12 14 13 8 14 9 8 11 8 6 5 4

%ID 63 53 95 56 61 60 47 52 51 52 57 49 55 78 77 87 90 84 58 70 56 59 46 76 53 57 83 58 50 48 55 48

Primer coverage

Domain

Primer coverage

Domain

CIDRα1.1

CIDRα1.2

CIDRα1.3

CIDRα1.4

CIDRα1.5

CIDRα1.6

CIDRα1.7

CIDRα1.8

CIDRα2.2

CIDRα2.3

CIDRα2.5

CIDRα2.6

CIDRα2.7

CIDRα2.9

CIDRα2.10

CIDRα2.1

CIDRα2.11

CIDRα2.4

CIDRα2.8

CIDRα3.1

CIDRα3.2

CIDRα3.3

CIDRα3.4

CIDRα3.5

CIDRα4

CIDRα5

CIDRα6

# # # # # # # 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

# Obs 9 7 5 10 6 8 8 3 15 12 8 8 8 6 5 16 5 12 7 63 36 8 19 3 9 13 10

%ID 71 94 98 60 61 61 67 56 48 55 59 55 53 55 57 48 52 50 52 49 50 56 53 85 47 48 49

Primer coverage

Primer coverage

Domain

Domain

CIDRβ1

CIDRβ2

CIDRβ3

CIDRβ4

CIDRβ5

CIDRβ6

CIDRβ7

CIDRγ1

CIDRγ2

CIDRγ3

CIDRγ4

CIDRγ5

CIDRγ6

CIDRγ7

CIDRγ8

CIDRγ9

CIDRγ10

CIDRγ11

CIDRγ12

CID

Rpam

CIDRδ1

CIDRδ2

# # # # # 0 0 # 9 # # 9 # 0 # 0 0 0 0 0 85 86

# Obs ## 12 11 15 19 15 3 12 13 8 11 11 9 8 7 6 5 5 4 11 14 8

%ID 44 54 55 47 51 51 56 50 55 46 57 57 53 57 57 60 58 66 59 75 63 66

Primer coveragePrimer coverage

Domain cassette

DC

1

DC

2

DC

3

DC

5

DC

6

DC

7

DC

8

DC

9

DC

10

DC

11*

DC

12

DC

13

DC

14*

DC

15*

DC

16

DC

17*

DC

18

DC

19

DC

20

DC

21

DC

22

All

genes

Primer coverage

CIDRδ

DBLα1

CIDRα

DBLδ

CIDRγ

Group

Group

CIDRα2

CIDRβ

Group

CIDRα3

Group

Group

DBLδ DBLγ

Figure S2. Overview of the 42 var subtype Q-PCR primer pair’s coverage of the global var sequence diversity.

The global coverage (figure S1) of major or minor domain subtypes is given as a increasing blue bar (0-100%)

under each domain type or domain cassette (13). A color was added under domain classes where at least 25% of

the observed domains were found in UPSA (green), UPSB (red), UPSC (yellow) or UPSE (pink). #Obs indicate

of domain type occurrences in 399 PfEMP1 sequences and %ID, the shared sequence identity of the domain type.

The likelihood of any var gene being targeted by one of the 42 primers is 90% (“all genes ”). *) Domain

cassettes not targeted by a primer pair exclusive for the domain cassette.

DBLα0

DBLβ DBLε

CIDRα1

DBLα S1 NJ tree

DB

La

0.1

3 D

2 ig

h v

ar3

8D

BL

a0.1

3 D

2 r

a j11

6 va

r14

96D

BL

a 0.1

9 D

2 P

FL

2 665

cD

BL

a0.5

D2

IT4v

ar1

0D

BL

a0.1

1 D

2 H

B3 v

ar4

7D

BLa

0 .11

D2

HB

3va r

910

0D

BLa

0.1

1 D

2 P

FA

0005

wD

BLa

0.16

D2

PF

L09

35c

DB

La0

.5 D

2 ig

h va

r17

DB

La0

.5 D

2 D

D2v

ar31

DB

La0

.5 D

2 D

D2v

ar4

6D

BLa

0.5

D2

PF

CLI

Nva

r44

94D

BLa

0.5

D2

DD

2var

18D

BL

a0.5

D2

IT4v

ar39

56

DB

La0.

16 D

2 ig

h va

r40

DB

La0.

5 D

2 ra

j116

var

31

DB

La0.

5 D

2 ig

h va

r29

6058

DB

La0.

19 D

2 D

D2v

ar16

DB

La0.

16 D

2 P

FB

1055

c

DB

La0.

13 D

2 P

FC

LIN

var2

5

DB

La0.

19 D

2 IT

var6

6

DB

La0.

19 D

2 P

FCLI

Nva

r64

100

72

DB

La0.

5 D

2 P

RE

ICH

var6

1

DB

La0.

13 D

2 ra

j116

var

30

DB

La0.

5 D

2 P

FCLI

Nva

r55

DB

La0.

5 D

2 ra

j116

var

9

DB

La0.

13 D

2 ra

j116

var

1

DB

La0.

13 D

2 ra

j116

var

23

100

DB

La0.

13 D

2 ra

j116

var

36

64

DB

La0.

22 D

2 ig

h va

r36

DB

La0.

5 D

2 H

B3v

ar20

DB

La0.

5 D

2 P

FA07

65c

DB

La0.

5 D

2 ig

h va

r7

DB

La0.

16 D

2 PF

07 0

139

DB

La0.

16 D

2 PF

CLI

Nva

r26

DBL

a0.5

D2

raj1

16 v

ar27

DBL

a0.5

D2

PFC

LIN

var6

6

DBL

a0.5

D2

PFD00

05w

DBLa0

.19

D2 HB3

var2

8

DBLa0

.15

D2 ra

j116

var

34

DBLa0.

5 D2

igh

var3

3

DBLa0.

5 D2

igh

var3

84

DBLa0.

16 D

2 PR

EICH

var9

3

DBLa0.

5 D2

IT4v

ar45

/var

61

DBLa0.

1 D2

PFD09

95c

DBLa0.

1 D2

PFD1000

c

100

DBLa0.

15 D

2 PF11

000

7

DBLa0.

15 D

2 PFCLI

Nvar8

DBLa0.

16 D

2 IT4v

ar44

DBLa0.1

6 D2

PF13 036

4

72

DBLa0.5 D

2 IT4v

ar5

DBLa0.1 D

2 MAL7P

1.50

DBLa0.15 D

2 DD2va

r10

DBLa0.1 D

2 HB3va

r27

DBLa0.1 D2 P

FCLINva

r65

100

DBLa0.1 D2 I

Tvar59

88

DBLa0.1 D2 P

F07 0051

50

DBLa0.15 D2 ig

h var1

DBLa0.1 D2 PREICHvar90

DBLa0.1 D2 IT4var47

DBLa0.22 D2 DD2var50

DBLa0.1 D2 PF07 0048

DBLa0.15 D2 raj116 var28

DBLa0.1 D2 HB3var50

DBLa0.1 D2 PFCLINvar56



100DBLa0.15 D2 raj116 var26

100

DBLa0.1 D2 DD2var34

DBLa0.1 D2 DD2var51

DBLa0.1 D2 ITvar68

DBLa0.1 D2 MAL8P1.220


DBLa0.1 D2 HB3var30

DBLa0.1 D2 HB3var31


DBLa0.1 D2 PFD0630c

DBLa0.1 D2 PFD0635c

100 DBLa0.1 D2 igh var31

DBLa0.20 D2 PF10 0001

DBLa0.20 D2 PFL1960w

DBLa0.14 D2 PFD1245c

DBLa0.1 D2 IT4var34


DBLa0.14 D2 igh var13


50DBLa0.15 D2 HB3var32

DBLa0.20 D2 PFCLINvar798DBLa0.20 D2 MAL7P1.5694DBLa0.22 D2 igh var18DBLa0.19 D2 DD2var26DBLa0.16 D2 dd2var7

100DBLa0.16 D2 DD2var41DBLa0.11 D2 igh var35DBLa0.16 D2 DD2var29DBLa0.16 D2 PFL1955w9856DBLa0.9 D2 DD2var23

DBLa0.9 D2 raj116 var29DBLa0.9 D2 PFCLINvar40DBLa0.9 D2 raj116 var6 DBLa0.9 D2 PFCLINvar67 DBLa0.9 D2 IT4var61 DBLa0.9 D2 MAL7P1.55 DBLa0.9 D2 PF10 0406 DBLa0.15 D2 DD2var24

66 58

DBLa0.10 D2 PREICHvar35DBLa0.8 D2 MAL6P1.1

62

DBLa0.8 D2 igh var15DBLa0.4 D2 IT4var16DBLa0.9 D2 PFL0005wDBLa0.9 D2 HB3var7DBLa0.7 D2 HB3var12DBLa0.8 D2 HB3var22

DBLa0.8 D2 PFD1005c

DBLa0.8 D2 AY028643

DBLa0.8 D2 DD2var13

DBLa0.8 D2 IT4var15

9674



DBLa0.23 D2 IT4var26



DBLa0.7 D2 HB3var23

DBLa0.7 D2 PFL1950w

100

DBLa0.7 D2 IT4var63

DBLa0.7 D2 PFB0010w

DBLa0.7 D2 HB3var14

DBLa0.7 D2 HB3var40

94


DBLa0.8 D2 HB3var26

DBLa0.23 D2 HB3var25

DBLa0.4 D2 DD2var15

DBLa0.4 D2 IT4var28

60


54

DBLa0.4 D2 HB3var36

100


DBLa0.1 D2 PFI1830c

DBLa0.1 D2 IT4var62



DBLa0.1 D2 IT4var58

DBLa0.1 D2 IT4var21

88



DBLa0.1 D2 IT4var54

DBLa0.1 D2 PFD0625c

52

DBLa2 D2 igh var19


84


DBLa0.1 D2 IT4var23


98


DBLa0.17 D2 PF07 0049

72

DBLa0.17 D

2 PFCLIN

var47

DBLa0.1 D

2 HB3var35

DBLa0.1 D

2 raj116 var13

DB

La0.15 D2 PFC

LINvar43

DB

La0.1 D2 PFC

LINvar39

DBLa0.1 D

2 PR

EIC

Hvar67

DB

La0.15 D2 PF08 0107

DB

La0.1 D2 P

RE

ICH

var62

DB

La0.17 D2 PFC

LINvar37

DB

La0.2 D2 P

F08 0106

DB

La0.1 D2 D

D2var45

54

DB

La0.17 D2 P

FCLIN

var41

DB

La0.15 D2 H

B3var13

50

DB

La0.17 D2 M

AL7P

1.212

DB

La0.17 D2 P

FCLIN

var24

DB

La0.17 D2 IT4var51

DB

La0.2 D2 D

D2var35

DB

La0.2 D2 H

B3var29

88

DBLα of DC8DBLα1 types

DBLa0.16 D2 DD2var29DBLa0.16 D2 PFL1955wDBLa0.16 D2 PFI0005w5460 DBLa0.11 D2 igh var16DBLa0.11 D2 IT4var25

98

DBLa0.11 D2 PF13 0001DBLa0.11 D2 PFE0005w100

56

DBLa0.11 D2 IT4var33DBLa0.11 D2 AAB60251DBLa0.11 D2 IT4var17

88

DBLa2 D2 MAL6P1.316DBLa0.1 D2 igh var4DBLa0.1 D2 HB3var24




DBLa1.4 D2 HB3var2

84DBLa1.4 D2 PFCLINvar48

DBLa1.2 D2 DD2var42

DBLa1.5 D2 Dd2var3

DBLa1.5 D2 AAQ73927

58



82

DBLa1.4 D2 PFD1235w


64


DBLa1.5 D2 DD2var9b

86

DBLa1.7 D2 IT4var7


DBLa1.7 D2 PF11 0521

DBLa1.7 D2 IT4var8

66

86

58


DBLa1.6 D2 HB3var1

DBLa1.6 D2 Dd2var4

DBLa1.6 D2 PF08 0141

100

DBLa1.5 D2 PF11 0008

DBLa1.7 D2 DD2var32

86

DBLa1.5 D2 IT4var2


DBLa1.7 D2 AAQ73928

DBLa1.7 D2 HB3var3

56

98

86

92

DBLa1.1 D2 HB3var1csa

DBLa1.1 D2 TM284var3

50


50

DBLa1.1 D2 DD2var25

DBLa1.1 D2 AAM55194

DBLa1.1 D2 AJ420411

DBLa1.1 D2 IT4var35

98


66

DBLa1.4 D2 PFE1640w



88

100


DBLa1.4 D2 IT4var22

DBLa1.5 D2 H

B3var6

98

DBLa1.6 D

2 PFCLIN

var69

DB

La1.5 D2 D

D2var52

DB

La1.5 D2 IT4var64

100

78

92

DB

La1.8 D2 IT4var60

DB

La1.2 D2 D

D2var40

DB

La1.6 D2 P

F13 0003

94D

BLa1.8 D

2 raj116 var5

DB

La1.6 D2 raj116 var16

DB

La1.6 D2 H

B3var5

96

96

DB

La1.2 D2 igh var30

DB


DB

La1.8 D2 igh var22

DB

La1.8 D2 igh var12

64D

BLa1.2 D

2 raj116 var3

DB

La1.6 D2 IT4var9

DB

La1.1 D2 igh var26

DB


52D

BLa1.2 D

2 PF

D0020c

DB

La1.1 D2 P

FC

LINvar49

DB

La1.2 D2 P

FC

LINvar32

DB

La1.6 D2 D

D2var22

DB

La1.2 D2 D

d2var9a

DB

La1.2 D2 D

D2var49

100

DB

La1.1 D2 P

FC

LIN

var68

DB

La1.1 D

2 igh var39

98D

BLa1.2 D

2 DD

2var43

58D

BLa1

.2 D2 P

FC

LINvar6

2

DB

La1.8

D2 ig h var24

76D

BL

a1.2 D2 ig

h var32

DB

La1. 2 D

2 igh var9

88D

BL a1.2 D

2 IT4va

r 18

DB

La1

.5 D

2 HB

3var4

50

DB

La0

.6 D

2 DD

2va r1b

DB

L a0. 6 D2 I T

4var2 7

DB

La0

. 6 D2 D

D2var1a

100D

BL

a0.6 D

2 DD

2v ar 3052

DB

La0 .18 D

2 PF

07 0050

DB

La

0.18 D

2 PFC

LIN

var6

1D

BL

a0.18 D

2 raj116 var35

100

72D

BL

a0.6

D2 D

D2va

r48D

BLa2 D

2 PF

08 0140

DB

La2 D2 P

FC

LINva

r31

80

DB

La2

D2 D

D2

var47D

BLa2 D

2 IT4va

r20

100

DB

La2

D2 raj116 va

r11D

BL

a2 D2 P

FC

LINvar3

0D

BLa0

.18 D

2 AA

C47438

DB

La0.18 D2

IT4

var12

100

DB

La0.18 D

2 IT4var1

DB


100

DB

La0.18 D2 P

FC

LINvar52

DB

La0.18 D2 igh var34

DB

La0.18 D2 P

FL0020w

100

62

DB

La2 D2 raj116 var8

DB

La0.8 D2 igh var37

DB

La0.6 D2 P

RE

ICH

var54

DB

La2 D2 IT4var19

DB

La2 D2 IT4var32b

100

DB

La2 D2 IT4var6

DB


70

84

DB

La0.18 D2 P

FCLIN

var6

DB

La0.12 D2 P

F08 0103

DB

La0.12 D2 H

B3var39

DB

La0.12 D2 H

B3var19

76

DB

La0.4 D2 D

D2var28

DB

La0.4 D2 H

B3var21

100

DB

La0.4 D2 igh var20

DB

La0.4 D2 H

B3var48

DB

La0.4 D2 igh var28

96

DB

La0.4 D2 H

B3var34

DB


92

60

DBLa0.12 D

2 raj116 var22

DB


DBLa0.21 D

2 DD

2var38

DBLa0.21 D

2 PFCLIN

var58

100




86


84



50




DBLa0.6 D2 HB3var17




DBLa0.6 D2 HB3var10

DBLa0.6 D2 MAL6P1.4

82


DBLa0.3 D2 IT4var29

DBLa0.3 D2 DD2var37

DBLa0.3 D2 IT4var11



98

DBLa0.3 D2 igh var5

60

DBLa0.4 D2 IT4var41


64



DBLa0.3 D2 DD2var21

DBLa0.3 D2 IT4var13

96




100



9884

DBLa0.9 D2 PF08 0142


DBLa0.9 D2 PFC1120c




74



DBLa0.9 D2 HB3var16



DBLa0.23 D2 ITvar32

100



DBLa0.9 D2 DD2var33 62

DBLa0.9 D2 HB3var8

DBLa0.9 D2 TM284S2var1

DBLa0.9 D2 PFC0005w


DBLa0.9 D2 DD2var20

82

DBLa0.9 D2 DD2var23DBLa0.9 D2 raj116 var29

DBLα S2 NJ tree

DB

La0

.14

D2

igh

var1

3D

BL

a0.1

5 D

2 P

F0

8 0 1

07D

BL

a 0.1

5 D

2 H

B3v

ar1

3D

BLa

0.1

7 D

2 P

FD

0615

cD

BLa

0.1

5 D

2 D

D2v

ar1 0

53D

BLa

0.1

D2

PR

EIC

Hva

r90

DB

La0.

1 D

2 IT

var6

8D

BLa

0.1

D2

PF

CLI

Nva

r 46

DB

La0.

15

D2

igh

var1

DB

La0.

14 D

2 P

FC

LIN

var1

1D

BL

a0.1

4 D

2 ig

h va

r8D

BLa

0.20

D2

PF

L196

0wD

BLa

0.1

D2

PF

D06

35c

DB

La0.

1 D

2 P

FD

0625

c

DB

La0.

1 D

2 P

FD

0630

c

7383

DB

La0.

17 D

2 IT

4var

51

DB

La0.

17 D

2 P

FC

LIN

var3

7

DB

La0.

1 D

2 D

D2v

ar51

DB

La0.

1 D

2 ra

j116

var

13

DB

La0.

1 D

2 D

D2v

ar45

DB

La0.

1 D

2 P

FCLI

Nva

r4

DB

La0.

1 D

2 D

D2v

ar34

DB

La0.

1 D

2 P

FCLI

Nva

r22

DB

La0.

1 D

2 P

RE

ICH

var6

2

DB

La0.

1 D

2 P

RE

ICH

var6

7

DB

La0.

1 D

2 IT

4var

54

DB

La0.

1 D

2 ig

h va

r31

DB

La0.

1 D

2 IT

4var

58

DB

La0.

1 D

2 IT

4var

21

99

DB

La0.

1 D

2 IT

4var

23

DB

La0.

1 D

2 H

B3v

ar27

DB

La0.

1 D

2 P

FCLI

Nva

r65

DB

La0.

1 D

2 IT

var5

9

99

99

91

95

DBL

a0.1

4 D

2 PF

D12

45c

DBL

a0.1

7 D

2 PF

CLI

Nva

r24

67

DBL

a0.2

2 D

2 H

B3v

ar33

DBL

a0.2

0 D

2 PF

CLI

Nva

r23

DBL

a0.1

D2

PF07

004

8

DBLa0

.1 D

2 HB3

var3

5

DBL

a0.1

7 D

2 PF

07 0

049

DBLa0.

17 D

2 PF

CLI

Nvar4

1

DBLa0.

17 D

2 PF

CLINva

r47

81

85

DBLa0.

1 D2

MAL8

P1.22

0

DBLa0.

1 D2

HB3var

30

DBLa0.

1 D2

PFI18

30c

DBLa0.

24 D

2 PFD

1015

c

DBLa0.

24 D

2 igh

var6

51

DBLa0.1

D2

PF07 0

051

DBLa0.2

0 D2

PF10 0

001

DBLa0.1

D2 I

T4var4

7

DBLa0.1

D2 MAL7

P1.50

DBLa0.24 D

2 DD2va

r12

DBLa0.1 D

2 PFD099

5c

DBLa0.1 D

2 PFD1000c

99

DBLa0.1 D

2 IT4va

r34

DBLa0.22 D2 ig

h var18

DBLa0.20 D

2 raj11

6 var2

1

DBLa0.11 D2 ig

h var16


61DBLa0.5 D2 PREICHva

r61

DBLa0.13 D2 raj116 va

r36



99

DBLa0.5 D2 DD2var46


99DBLa0.5 D2 DD2var18

DBLa0.1 D2 IT4var62

DBLa0.5 D2 IT4var45/var61




99 DBLa0.5 D2 DD2var31

DBLa0.5 D2 igh var7

DBLa0.16 D2 PF13 0364


DBLa0.15 D2 PF11 0007


59 DBLa0.5 D2 IT4var10

DBLa0.11 D2 PFA0005w


DBLa0.11 D2 HB3var9

9969

DBLa0.1 D2 HB3var31

DBLa0.1 D2 HB3var50



DBLa0.5 D2 PFD0005w


DBLa0.5 D2 IT4var39



DBLa0.5 D2 IT4var5DBLa0.19 D2 PFL2665cDBLa0.5 D2 igh var33DBLa0.16 D2 PREICHvar93DBLa0.5 D2 igh var3DBLa0.16 D2 PF07 0139DBLa0.13 D2 PFCLINvar25DBLa0.19 D2 ITvar66DBLa0.19 D2 PFCLINvar64

99

DBLa0.13 D1 IT4var30DBLa0.16 D2 PFB1055c

DBLa2 D2 PF08 0140

DBLa2 D2 raj116 var11

DBLa2 D2 PFCLINvar31DBLa2 D2 DD2var47DBLa2 D2 IT4var20

99DBLa1.7 D2 HB3var3DBLa1.7 D2 igh var27

97

DBLa1.7 D2 AAQ73928

87

DBLa1.5 D2 PFCLINvar75 DBLa1.5 D2 Dd2var3 DBLa1.4 D2 PREICHvar71 DBLa1.4 D2 PFCLINvar48 DBLa1.4 D2 PFD1235w

55DBLa1.6 D2 HB3var1 DBLa1.6 D2 Dd2var4 DBLa1.6 D2 PF08 0141

9993

DBLa1.4 D2 igh var10DBLa1.2 D2 igh var30

77

DBLa1.4 D2 HB3var2DBLa1.7 D2 DD2var32DBLa1.4 D2 PFCLINvar73

57

DBLa1.4 D2 PFE1640w



DBLa1.1 D1 AF547159

5799

53


DBLa1.5 D2 PF11 0008


DBLa1.5 D2 AAQ73927

DBLa1.5 D2 DD2var9b

DBLa1.5 D2 DD2var52

DBLa1.5 D2 IT4var64

99



DBLa1.7 D2 IT4var7

DBLa1.7 D2 IT4var8

97

DBLa1.7 D2 PF11 0521

DBLa1.4 D2 IT4var22


75

DBLa1.5 D2 HB3var6

DBLa1.1 D2 AAM55194

DBLa1.1 D2 TM284var3

99

DBLa1.1 D2 igh va

r11

69

DBLa1.1 D2 AJ420411

DBLa1.1 D2 D

D2var25

93

99

DBLa1.1 D2 IT

4var35

91

DBLa1.1 D

2 HB3va

r1csa

95

DBLa1.2 D

2 raj116

var3

DBLa1.2

D2 IT4v

ar18

DBLa1.2

D2 P

FD0020

c

75

DBLa1.1

D2 igh v

ar26

99

DBLa1.1

D2

igh va

r14

DBLa1.

1 D2 P

FCLINva

r68

DBLa1.

5 D2 I

T4var

2

DBLa1.

5 D2

raj11

6 va

r7

DBLa1.

2 D2

DD2var

42

DBLa1.

8 D2

raj1

16 va

r38

71

DBLa1.

8 D2

igh

var2

2

DBLa1.

8 D2

raj1

16 va

r5

DBLa1

.6 D

2 PF1

3 00

03

DBLa1.

6 D2

raj1

16 v

ar16

97

DBLa1

.2 D

2 DD

2var

40

DBL

a1.2

D2

Dd2

var9

a

9967

DBL

a1.6

D2

HB3

var5

DBL

a1.8

D2

IT4v

ar60

DBL

a1.1

D2

igh

var3

9

DBL

a1.6

D2

raj1

16 v

ar19

DB

La1.

8 D

2 ig

h va

r12

DB

La1.

6 D

2 IT

4var

9

DB

La1.

8 D

2 ig

h va

r24

DB

La1.

6 D

2 ra

j116

var

17

DB

La1.

2 D

2 P

FCLI

Nva

r62

DB

La1.

2 D

2 P

FCLI

Nva

r32

DB

La1.

2 D

2 D

D2v

ar49

DB

La1.

2 D

2 D

D2v

ar43

DB

La1.

6 D

2 D

D2v

ar22

DB

La1.

5 D

2 H

B3v

ar4

DB

La1.

1 D

2 P

FCLI

Nva

r49

DB

La1.

2 D

2 ig

h va

r32

DB

La1.

2 D

2 ig

h va

r9

97

63

95

DBLα of DC8DBLα1 types

DBLa0.19 D2 PFCLINvar64DBLa0.13 D1 IT4var30DBLa0.16 D2 PFB1055cDBLa0.5 D2 igh var1763 DBLa0.16 D2 PFL0935c

DBLa0.5 D2 raj116 var9DBLa0.5 D2 PFA0765cDBLa0.5 D2 PFCLINvar66DBLa0.16 D2 IT4var44DBLa0.16 D2 PFCLINvar26

DBLa0.5 D2 HB3var20




DBLa0.16 D2 PFI0005w

DBLa0.11 D2 PF13 0001

DBLa0.11 D2 PFE0005w

99

DBLa0.11 D2 AAB60251





DBLa0.16 D2 dd2var7

99



DBLa0.7 D2 PFB0010w




97


51




99

59





DBLa0.9 D2 PFC1120c


95


DBLa0.2 D2 PF08 0106

67

DBLa0.2 D2 DD2var35

DBLa0.2 D2 HB3var29

99



71


DBLa0.9 D2 HB3var16




51


DBLa0.9 D2 PFCLIN

var67

DBLa0.9 D2 TM

284S2var1

DBLa0.9 D2 D

D2var20

DBLa0.9 D

2 PF10 0406

DBLa0.9 D

2 raj116 var10

DB

La0.9 D2 D

D2var33

DB

La0.9 D2 P

FC0005w

99

DB

La0.9 D2 H

B3var8

DB


69

DB

La0.9 D2 igh var25

DB

La0.9 D2 IT4var61

87D

BLa0.23 D

2 IT4var14

DB

La0.23 D2 ITvar32

9975

DB

La0.9 D2 P

RE

ICH

var55

DB

La0.8 D2 igh var15

DB


DB

La0.8 D2 H

B3var22

DB

La0.8 D2 P

FCLIN

var15

DB

La0.8 D1 IT4var36

DB

La0.8 D1 P

FC

LINvar77

DB

La0.8 D2 P

FC

LINvar57

DB

La0.8 D2 P

RE

ICH

var53

DB

La0.8 D2 P

RE

ICH

var85

DB

La0.8 D2 igh var37

DB

La0.23 D2 D

D2var44

DB

La0.23 D2 IT

4var26

DB

La0 .15 D2 D

D2var24

DB

La0.9 D2 M

AL7P

1.55

DB


DB

La0.9 D

2 DD

2var23

DB

La0.8 D2 D

D2var13

DB

La0.8 D2 IT

4v ar1599

DB

La0.8 D2 H

B3var2 6

87D

BL

a0.8 D

2 PF

D1005c

55D

BLa0

.8 D

2 MA

L6P1.1

55D

BL

a0 .8 D2 A

Y0286 43

DB

La0. 8 D

2 PR

EI C

Hvar92

DB

La 0 .6 D

2 PR

EIC

Hva r5 2

DB

La0.21 D

2 D

D2v ar3

6D

BLa

0.21 D

2 M

AL6

P1.252

83D

BLa0.2

1 D2 P

FC

LINvar60

9 9

DB

La0.15 D

2 HB

3var18

91

DB

La0.12 D

2 P

RE

ICH

var83

DB

La0.6 D2 H

B3var17

DB

La0.6 D2 P

FC

LINvar53

99

DB

La0.1

2 D2

PF

CLIN

var45

DB

La0.4 D2 H

B3var21

61

DB

La0.21 D2 D

D2var3

8D

BLa0.2

1 D2 P

FC

LINvar58

63

DB

La0.12 D2 H

B3var39

DB

La0.12 D2 igh var21

DB

La0.12 D2 H

B3var11

DB

La0.12 D2 H

B3var19

DB

La0.21 D2 P

FC

LINvar28

DB

La0.12 D2 P

F08 0103

DB

La2 D2 IT

4var19

DB

La2 D2 IT

4var32b

99

DB

La2 D2 IT4var6

DB

La2 D2 P

FCLIN

var30

DB


65

DB

La0.6 D2 P

RE

ICH

var54

DB

La0.7 D2 H

B3var23

DB

La0.7 D2 P

FL1950w

99

DB

La0.7 D2 H

B3var12

DB

La0.7 D2 IT4var63

DB

La0.7 D2 H

B3var14

DB

La0.7 D2 H

B3var40

9987

DB


DB

La0.6 D2 D

D2var30

59

DB

La0.6 D2 H

B3var10

DB

La0.6 D2 M

AL6P

1.4

51

DBLa0.9 D

2 HB

3var7

DB

La0.9 D2 P

F08 0142

51

DBLa0.6 D

2 raj116 var18

DB

La0.6 D2 D

D2var48

51

DBLa0.6 D

2 DD

2var1a

DBLa0.6 D2 D

D2var1b

DBLa0.6 D

2 IT4var27

99

99

DBLa0.9 D2 PFL0005w

DBLa0.4 D2 HB3var36

DBLa0.4 D2 IT4var28

DBLa0.4 D2 DD2var15

83


99


91





93

DBLa0.18 D2 IT4var1


99 69


57


DBLa0.4 D2 DD2var28

79

DBLa0.4 D2 HB3var48

73


DBLa0.18 D2 PF07 0050

59




99



99


97



DBLa0.1 D2 igh var4


DBLa0.1 D2 HB3var24DBLa0.18 D2 AAC47438


99

DBLa2 D2 igh var19

DBLa0.3 D2 DD2var21

DBLa0.3 D2 IT4var13

99DBLa0.3 D2 PFCLINvar71

95

DBLa0.3 D2 IT4var11


89


DBLa0.3 D2 igh var5

57

DBLa0.3 D2 DD2var37


85

DBLa2 D2 MAL6P1.316

DBLa0.4 D2 IT4var16

DBLa0.4 D2 IT4var41

99

DBLa0.4 D2 HB3var34

DBLa0.12 D2 raj116 var37 83

DBLa0.3 D2 IT4var29



DBLa2 D2 PF08 0140


DBLa2 D2 PFCLINvar31

DBLα S3 NJ tree

DB

La0.

1 D

2 P

FD09

95c

DB

La0.

1 D

2 P

FD

1000

c

99D

BL a

0.1

D2

DD

2var

45

57

DB

La0.

1 D

2 i g

h va

r31

DB

La0.

1 D

2 H

B3 v

ar35

DB

La0 .

1 D

2 H

B3v

ar3 1

6357

DB

La0.

1 D

2 P

FC

LIN

var4

6D

BLa

0.1

D2

IT4v

ar34

DB

La0.

1 D

2 P

FC

LIN

var2

2D

BLa

0.1

D2

PFC

LIN

var5

6D

BLa

0.1

D2

MA

L8P

1.22

0D

BLa

0.1

D2

PF

CLI

Nva

r4D

BLa

0.1

D2

IT4v

ar62

97D

BLa

0.1

D2

PF

CLI

Nva

r39

DB

La0.

17 D

2 P

F07

0049

DB

La0.

1 D

2 IT

4var

21

DB

La0.

1 D

2 H

B3v

ar27

DB

La0.

1 D

2 P

FCLI

Nva

r65

DB

La0.

1 D

2 IT

var5

9

9199

DB

La0.

1 D

2 M

AL7

P1.

50

DB

La0.

3 D

2 D

D2v

ar37

DB

La0.

23 D

2 H

B3v

ar25

DB

La0.

17 D

2 P

FD06

15c

DB

La0.

1 D

2 D

D2v

ar34

DB

La0.

24 D

2 ig

h va

r6

DB

La0.

24 D

2 D

D2v

ar12

DB

La0.

2 D

2 D

D2v

ar35

DB

La0.

1 D

2 IT

4var

54

DB

La0.

17 D

2 PF

CLI

Nva

r37

93D

BLa

0.20

D2

raj1

16 v

ar21

DB

La0.

20 D

2 PF

CLI

Nva

r7

83

DBL

a0.2

0 D

2 PF

CLI

Nva

r23

DB

La0.

20 D

2 PF

10 0

001

DBL

a0.2

0 D

2 PF

L196

0w

DBL

a0.4

D2

DD

2var

15

DBL

a0.4

D2

IT4v

ar28

99

DBL

a0.4

D2

PFC

LIN

var6

3

99

DBL

a0.3

D2

PFCL

INva

r9

DBL

a0.1

D2

PREI

CHv

ar67

DBLa0

.23

D2 IT

4var

26

DBLa0

.1 D

2 PR

EICHv

ar62

DBLa0

.22

D2 HB3v

ar33

DBLa0.

2 D2

PF08

0106

DBLa0.

6 D2

PFCLI

Nvar5

3

DBLa0.

1 D2

raj11

6 va

r13

DBLa0.

1 D2

IT4v

ar23

DBLa0.

1 D2

IT4v

ar58

99

DBLa0.

6 D2

PREICHva

r52

DBLa0.6

D2

PREICHva

r54

DBLa0.6

D2 DD2v

ar30

DBLa0.6

D2 H

B3var

10

DBLa0.1 D

2 PREIC

Hvar90

DBLa0.1

D2 PF07

0048

DBLa0.1 D

2 ITva

r68

DBLa0.22 D2 ig

h var18

DBLa0.22 D

2 igh va

r36

DBLa0.1 D2 H

B3var50

DBLa0.1 D

2 PFI1830c

DBLa0.22 D2 D

D2var50

DBLa0.6 D2 MAL6P1.4

DBLa0.6 D2 DD2var1a

DBLa0.6 D2 DD2var1b

DBLa0.6 D2 IT4var27

99


DBLa0.6 D2 HB3var17

51DBLa0.1 D2 DD2var51

DBLa0.1 D2 HB3var24

DBLa0.3 D2 igh var5


DBLa0.6 D2 DD2var48

DBLa0.2 D2 HB3var29


DBLa0.3 D2 IT4var29

DBLa0.23 D2 ITvar32

9971

DBLa0.9 D2 DD2var23


53

DBLa0.1 D2 igh var4





87DBLa0.17 D2 MAL7P1.212

DBLa0.1 D2 IT4var47

DBLa0.1 D2 PFD0630c

DBLa0.1 D2 PFD0635c

99DBLa0.1 D2 PFD0625c

DBLa0.1 D2 PF07 0051




DBLa0.1 D2 HB3var30

DBLa0.7 D2 HB3var23

9783DBLa0.4 D2 IT4var16

DBLa0.4 D2 IT4var4199DBLa0.4 D2 igh var20DBLa0.4 D2 HB3var48DBLa0.4 D2 igh var28

99DBLa0.14 D2 igh var13DBLa0.4 D2 HB3var21DBLa0.4 D2 HB3var34DBLa0.4 D2 DD2var28957599 DBLa0.15 D2 PFCLINvar8DBLa0.15 D2 raj116 var34DBLa0.9 D2 PFL0005wDBLa0.9 D2 MAL7P1.55

93

DBLa0.9 D2 HB3var8DBLa0.15 D2 HB3var138799DBLa0.5 D2 PFCLINvar44

DBLa0.8 D2 AY028643

DBLa0.5 D2 igh var7

DBLa0.5 D2 DD2var31

85

DBLa0.5 D2 PFD0005wDBLa0.5 D2 IT4var5DBLa0.5 D2 PFCLINvar66

99


59

DBLa0.5 D2 PFA0765cDBLa0.5 D2 igh var29 DBLa0.5 D2 raj116 var27 DBLa0.5 D2 PFCLINvar55 DBLa0.5 D2 IT4var39 DBLa0.5 D2 IT4var45/var61 DBLa0.5 D2 igh var3 DBLa0.5 D2 raj116 var31

75DBLa0.8 D2 PFCLINvar57DBLa0.10 D2 PREICHvar31

51

DBLa0.10 D2 PREICHvar35DBLa0.3 D2 DD2var21DBLa0.3 D2 IT4var13

99


99



75


55




99

DBLa0.9 D2 PFC1120c

DBLa0.9 D2 HB3var7

DBLa0.11 D2 PF13 0001

DBLa0.11 D2 PFE0005w

99


71


61


51



67


83




81

59


73


99



DBLa0.18 D2 PF07 0050


DBLa0.18 D2 raj116 va

r33

DBLa0.18 D2 IT4va

r1

5597

69

DBLa0.12 D

2 IT4va

r40

DBLa0.12 D

2 raj11

6 var2

2

79

DBLa0.12 D

2 raj11

6 var37

99

DBLa0.23 D

2 IT4va

r14

DBLa0.3 D

2 IT4v

ar11

DBLa0.12 D

2 HB3v

ar19

DBLa0.12

D2 P

F08 010

3

81

DBLa0.1

1 D2 H

B3var4

7

DBLa0.1

1 D2

HB3var

9

99

DBLa0.

11 D

2 igh

var3

5

DBLa0.1

1 D2

PFA0005

w

DBLa0.

9 D2

PREICHva

r55

DBLa0.

10 D

2 DD2v

ar19

DBLa0.

5 D2

DD2var

18

99

DBLa0.

9 D2

PFCLI

Nvar2

9

DBLa0

.12

D2 igh

var

21

DBLa0

.12

D2 HB3v

ar11

DBLa0

.9 D

2 PF

C0005

w

DBLa0

.9 D

2 PF

CLIN

var4

0

DBL

a0.9

D2

raj1

16 v

ar15

DBLa

0.9

D2

DD2v

ar33

DBLa

0.9

D2

raj1

16 v

ar10

DBL

a0.9

D2

PFC

LIN

var6

7

DBL

a0.9

D2

DD

2var

20

DBL

a0.9

D2

raj1

16 v

ar6

DBL

a0.9

D2

PF1

0 04

06

DB

La0.

9 D

2 H

B3va

r16

DB

La0.

10 D

2 IT

4var

67

DB

La0.

10 D

2 PF

CLI

Nva

r27

57

DB

La0.

10 D

2 IT

4var

24

DB

La0.

10 D

2 IT

4var

46

DB

La0.

11 D

2 AA

B60

251

DB

La0.

12 D

2 ra

j116

var

4

DB

La0.

9 D

2 TM

284S

2var

1

DB

La0.

10 D

2 D

D2v

ar39

DB

La0.

10 D

2 ra

j116

var

39

9977

DBLα of DC8DBLα1 typesDBLα1 w/ CIDRβ/γ/δDBLα1 w/ CIDRα1

DBLa0.9 D2 MAL7P1.55DBLa0.9 D2 HB3var8DBLa0.15 D2 HB3var13DBLa0.9 D2 PF08 0142DBLa0.9 D2 IT4var61

63 DBLa0.15 D2 DD2var10DBLa0.15 D2 PFCLINvar43


87

DBLa0.15 D2 igh var1DBLa0.15 D2 PF11 0007

53


DBLa0.15 D2 PF08 0107





9999

DBLa0.8 D2 DD2var13

DBLa0.8 D2 IT4var15

99

DBLa0.8 D2 MAL6P1.1

DBLa0.8 D2 HB3var26

DBLa0.8 D2 PFD1005c

67

DBLa0.7 D2 PFL1950w

DBLa0.7 D2 HB3var14

DBLa0.7 D2 HB3var40

99DBLa0.7 D2 IT4var63



99



DBLa0.8 D1 IT4var36

63

83

DBLa0.19 D2 ITvar66


99

DBLa0.16 D2 dd2var7

85


DBLa0.16 D2 PFI0005w

99

59

DBLa0.4 D2 HB3var36


DBLa0.16 D2 PF13 0364

69



DBLa0.16 D2 PF07 0139

DBLa0.16 D2 PFB1055c


83


DBLa0.16 D2 PFL0935c




99

75



99DBLa0.18 D2 igh var2


9995

DBLa0.18 D2 AAC47438


9969

DBLa1.5 D2 DD

2var9b

DBLa1.5 D2 IT4var2

99D

BLa1.5 D2 AAQ

73927

61

DBLa1.5 D

2 HB3var6

DBLa1.5 D

2 DD

2var52

DBLa1.5 D

2 IT4var64

9985

DB

La1.5 D2 PFC

LINvar5

83

DB

La1.5 D2 P

FCLIN

var75

DB


77

55

DB

La1.5 D2 H

B3var4

DB

La1.5 D2 D

d2var3

99

75

DB

La1.8 D2 igh var22

DB

La1.8 D2 igh var12

59D

BLa1.8 D

2 IT4var60

DB

La1.8 D2 igh var24

6199

DB


DB

La1.7 D2 IT4var8

87D

BLa1.5 D

2 PF11 0008

DB


99

DB

La1.7 D2 P

FC

LINvar33

61

DB


DB

La1.6 D2 D

D2var22

DB

La1.6 D2 H

B3var5

95D

BLa1.6 D

2 raj116 var19

93D

BLa1.6 D

2 HB

3var1

DB

La1.6 D2 D

d2var4

57D

BLa1.6 D

2 PF08 0141

DB

La1.6 D2 P

F13 0003

DB

La1.6 D2 P

FC

LINvar69

9961

7375

891914 25D

BLa

DB

La1 .4 D2 igh v ar10

99D

BLa1 .6 D

2 IT4var9

DB


9977

1 91 4 1 2DB

La93

1965

2D

BLa

DB

La1.

2 D

2 D

D2v

ar43

99

1983

13D

BLa

7 7

DB

La1.

2 D

2 ra

j116

var

3D

BLa

1.2

D2

igh

var9

97

69

198 3

4D

BL a

77

DB

La1 .

2 D

2 D

D2 v

ar40

DB

La1.

2 D

2 D

d2va

r 9a

99

DB

La1 .

2 D

2 ig

h v a

r30

99

DB

La1.

1 D

2 ig

h v a

r14

55

DB

La1.

2 D

2 IT

4var

181 9

65 7

DB

La

DB

La1.

2 D

2 P

FC

LIN

var6

297

77

59

DB

La2

D2

raj1

16 v

ar11

DB

La2

D2

PF

08 0

140

89

DB

La2

D2

IT4v

ar19

DB

La2

D2

IT4v

ar32

b

99

DB

La2

D2

IT4v

ar6

99

DB

La2

D2

raj1

16 v

ar8

DB

La2

D2

igh

var1

9

DB

La1.

2 D

2 P

FD

0020

c

1983

3D

BLa

1983

1D

BLa

DB

La2

D2

PFC

LIN

var3

0

DB

La2

D2

MA

L6P

1.31

6

1965

1D

BLa

1965

3D

BLa

9989

DB

La2

D2

PFC

LIN

var3

1

DB

La2

D2

DD

2var

47

DB

La2

D2

IT4v

ar20

9969

DB

La1.

4 D

2 H

B3v

ar2

DB

La1.

4 D

2 P

FD12

35w

99

DB

La1.

2 D

2 ig

h va

r32

DB

La1.

2 D

2 D

D2v

ar49

DB

La1.

4 D

2 IT

4var

22

DB

La1.

2 D

2 D

D2v

ar42

DBL

a1.7

D2

IT4v

ar7

1965

8D

BLa

DBL

a1.4

D2

PFC

LIN

var7

3

95

1983

15D

BLa

DBL

a1.4

D2

PREI

CH

var7

1

DBL

a1.4

D2

PREI

CHv

ar43

DBLa1

.4 D

2 PF

E164

0w

DBLa

1.4

D2 P

FCLI

Nva

r76

DBLa1

.4 D

2 ra

j116

var

2

DBLa1

.1 D

1 AF

5471

59

9959

DBLa1.

1 D2

AJ420

411

DBLa1.

1 D2

DD2var

25

99

DBLa1.

1 D2

TM28

4var

3

83

DBLa1.

1 D2

HB3var

1csa

DBLa1.

1 D2

IT4v

ar35

85

99

DBLa1.

1 D2

AAM55

194

DBLa1.

1 D2 i

gh va

r11

99

99

DBLa1.4

D2 P

FCLINva

r48

DBLa1.2

D2 P

FCLINva

r32

1914 1

4DBLa

DBLa1.1 D

2 igh v

ar39

DBLa1.7 D

2 PF11

0521

DBLa1.1 D

2 PFCLIN

var68

DBLa1.7 D

2 igh va

r23

DBLa1.7 D2 D

D2var32

DBLa1.1 D2 ig

h var26

DBLa1.1 D2 PFCLIN

var49

95

DBLa1.7 D2 AAQ73928

DBLa1.7 D2 igh va

r27

1914 21DBLa

DBLa1.7 D2 HB3var3

65

87 81

6 7

DBLa0.7 D2 PFB0010w

DBLa0.7 D2 HB3var12

99

DBLa0.8 D2 HB3var22


97DBLa0.12 D2 HB3var39



99






95DBLa0.19 D2 PFL2665c


61





75






1983 9DBLa

99

DBLa0.5 D2 IT4var10

DBLa0.5 D2 HB3var20

DBLa0.5 D2 DD2var46

DBLa0.5 D2 PFCLINvar44 99DBLa0.8 D2 AY028643

DBLa0.5 D2 igh var7 85

Figure S3. DBL subdomain S1-3 NJ trees. DBLα of domain cassette 8 groups with DBLα0 types in

S1&2 but with DBLα1 types in S3.

10 20 30 40 50 60 70 80 90 100 110 120 130 140 150. . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . |

1965-1 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~1965-3 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~1983-1_ ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~1983-3 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~IT4var20 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~MAPAQGGTN~KTAKEVLDEIGKEV~QEKATEDALTYRNDLQGNLSQAKF~~~~HGVPIDVKNPCDLNYEIHTNVVKGRKKIT4var06 MAPPGPRGRGRGGGRGGGRGRGGGRGGGRGRGGGSSGGGGQGGRQVIRQAVVRWFRRGRGGGRGGGGGSVEDDAEKYKNAQDAKDLLDMIGETV~QEKVHREAADYRNALHGLWSNVTYPKDERRTGSTPSNPCQLNYKYHTNVTDGYGRMAL6P1.316 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~MAP~QRAEDSNKSAKEVLDEFGQQVYKEKVEKDAKIYKGELEGNLASSSILG~~~~ETASTDKPCTFEYNKLL~~GAR~GKPF08_0140 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~MVPPVRSPRAAGPAPAPASASTTYGSVKDLLEDIGKSIQKQAKDAAEKRSNGELQGDLSKARF~~~~KDQKNTTTNPCQLNYRNHSNVRSSFENPFCLINvar30~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~MGPSVRGDGKGRTARDVLEEFGGKIQEIAHTAALKRSESELKGLLSNATING~~~~VKNKATDPCQLDHKYET~~NVGTGKIT4var19 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~MGPKAAVTDYSDAKHLLDSIGEKVYKEKVEKKAVDYRSALQGRLSEASF~KDRKNIERGKAELCKLNHIYHTNVTDGYGRIT4var32b ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~MGPKAAVTDYSDAKHLLDSIGEKVYKEKVEKKAVDYRSALQGRLSEASF~KDRKNIERGKAELCKLNHIYHTNVTDGYGRDD2var47 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~MAPAQGGTN~KTAKEVLDEIGKEV~QEKATEDALTYRNDLQGNLSQAKF~~~~HGVPIDVKNPCDLNYEIHTNVVKGRKKighvar19 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~MALPLRPSSRGGGGGGSGGGKDKYDDAKEVLDEFGQQVYDEIVKKDAEKYKKALTGQLSLAST~~~~SPETVGTYKPCDFDYDEHTTSANG~~Nraj116var11~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~MVPPVRSPRAAGPAPAPASASTTYSSVKDLLEDIGKSIQKQAKDAAEKRSNGELQGDLSKARF~~~~KDGKNKATKLCELEHTKHTNAVNVPKDraj116var08~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~MAPGSTGTQ~DEDAKNMFDRIGGIIQQQVHDAALEYN~SQLLGHLSEAKFLHG~TSWEQANQNVCSLKHTHDT~~NVRWGE

160 170 180 190 200 210 220 230 240 250 260 270 280 290 300. . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . |

1965-1 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~XRSFADIGDIVRGKDLHLRHEPGI~~~~QHLEKRLESMFEKIQK1965-3 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~XRSFADIGDIIRGKDLHLRHEPGI~~~~QHLEKRLESMFANIQD1983-1_ ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ADIGDIIRGKDLYLDHEPGK~~~~QHLEERLEQMFENIKN1983-3 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~XRSFADIGDIIRGKDLYLDHEPGK~~~~QHLEERLERIFANIQNIT4var20 ENPCRGREEKRFSDVLSGQCAKNTIK~~~DSVTNSVGACAPFRRLHVCDRNLELIKTDENTSTHD~~~ILVDVLLTAKHEGESLVKHYKEYIKKNRNFN~~ICTVLARSFADIGDIVRGKDLYLGYDDEEKEKRKQLEKNLKKFFQKIHDIT4var06 EHPCKDRWDIRFSDKYGGQCTNEKIHGNEEKEGKDVGACAPFRRLHLCDQHLSHMNAGKTNTTDN~~~LLLEVCLAAKYEGESLVEKHKEFKKTHNDSN~~ICTILARSFADIGDIIRGKDLYRGG~~~~GRGRKQLEENLQKIFGNIYNMAL6P1.316 RHPCGNRQTVRFSDEYGGQCTFNRIKDS~EHNNNDVGACAPFRRLHLCDYNLEKMGSTKIKD~~~KNVLLAEVCMAAKYEGQSLLKQYEEHK~~NNYPHTNICTVLARSFADIGDIVRGKDLFLGHQ~~~~QRKRKLEENLKQMFENIK~PF08_0140 DNPCYGREEKRFSDSRSGQCTYNRIKDSKEGDNK~VGACAPYRRLHICDHNLENINDYKNINNHT~~~LLVDVCLSAKHEGEMIANKLKEYDK~~SNYESRICTVLARSFADIGDIVRGKDLYLGDQQE~~~~KLYLENNLKKIFAKIHEPFCLINvar30SDPCANRSKERFSDVLDGQCAKNTIKDS~VTN~~SVGACAPFRRLHLCDHHLSYMKAGKINS~~~KDNLLLEVCYAAKYEGKSLVKKYTEYKQQNNDSDTDICTVLARSFADIGDIIRGKDLYLGYDDKEKEQRKKLEDKLKEIFENIYNIT4var19 EHPCKDRWDIRFSDKYGGQCTNSKIHGNDDSNGKDIGACAPFRRLHLCDQHLSHMKAEKINSKDN~~~LLLEVCLAAQYEGESLVEKHKEFKKTHNDSN~~ICTILARSFADIGDIIRGKDLYLGN~~~~KKEKEKLENNLKKIFKEIYKIT4var32b EHPCKDRWDIRFSDKYGGQCTNSKIHGNDDSNGKDIGACAPFRRLHLCDQHLSHMKAEKINSKDN~~~LLLEVCLAAQYEGESLVEKHKEFKKTHNDSN~~ICTILARSFADIGDIIRGKDLYLGN~~~~KKEKEKLENNLKKIFKEIYKDD2var47 ENPCRGREEKRFSDVLSGQCAKNTIK~~~DSVTNSVGACAPFRRLHVCDRNLELIKTDENTSTHD~~~ILVDVLLTAKHEGESLVKHYKEYIKKNRNFN~~ICTVLARSFADIGDIVRGKDLYLGYDDEEKEKRKQLEKNLKKFFQKIHDighvar19 TKPCGNDGKGNDVDRFSVKEQAEYDNKKMKCSNS~EGACAPYRRLHLCSHNLESIDTDKIDSGNARHKLLAEVCMAAKYEGKSLVEKHKKFKQDNSDFNTNICTVLARSFADIGDIVRGKDLFLGNDKEKD~QRKKLDENLKTIFGNIYEraj116var11RDPCYGREEKRFSDERSGQCTYNRIKDSEIGNNN~IGACAPYRRLHLCHHNLETIETTS~MTTHD~~~LLLEVCMAAKYEGQSIRDYYPIYQTKYNDYGSTICTVLARSFADIGDIIRGKDLYLGYDDEEKNRRQQLEENLKKIFANIYKraj116var08SYPCAKRSEKRFSDEGGGECDDSKIKGN~KGS~~NGGACAPLRRLHLCVRNLEKMDANNYDSGKATHTLLAEVCMAAKYEGQSITGEYPKYQAKYGDSPSQICTMLARSFADIGDIIRGRDLYSGNS~KEKNRRKKLEENLKKIFGKIYD

310 320 330 340 350 360 370 380 390 400 410 420 430 440 450. . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . |

1965-1 NNNNKLSNLSTK~~~~~~~~~~~~~~~~~~~~EVREYWWALNRVQVWKAITCKAKEGDIYSKTANG~~~NTTLWNDNCGHHVNQDVPTNLDYVPQFLRWFEEWAEDFCRKKNKKLKDVKKYCRGDEGKGEVKYCSQNGFDCTRLIQNKDS1965-3 N~~TKLQNIPLP~~~~~~~~~~~~~~~~~~~~KVREYWWALNRKEVWKAITCGATMNDIFSKNIRNS~~RTTLFDYNCGHHKDNNVPTNLDYVPQFLRWFEEWAEDFCRKKNKKLKDVKKYCRGDEGKGEVKYCSQNGFDCTRLIQNKDS1983-1_ NN~EKLKDLPLD~~~~~~~~~~~~~~~~~~~~EVREYWWALNRVQVWKAITCNAEGTDKYFKKSSGG~~DYLFSGGKCGRNEEK~VPTYLDYVPQFLRWFDEWAEDFCRKRNIKLKLAMKECRG~~~EHKEKYCSQNGYDCTKRIKKGDS1983-3 KN~EKLKDLPLD~~~~~~~~~~~~~~~~~~~~EVREYWWELNRDQVWKAITCGATMKDISSKNIRDA~~KMILFHYNCGHHNDK~AQTYLDYVPQYLRWFEEWSEEFCRKRNNKLKLAKEACRN~~~DEQKLYCSQNGYDCTKRIKKGDSIT4var20 DVMKTSGRTNGKKSAEAQKRYN~DATGN~~YYKLREDWWNANRDQVWKAITCDAADNDEYFENSSDG~~LYVFSNGQCGRN~EGKVPTNLDYVPQHLRWFDEWAEDFCRKRNITLKSAKEKCGG~~~EGNTKYCSLNGYDCTKVFEKKDSIT4var06 ELTTS~~~~~GKNVDKAKERYKGDADNN~~YFQLREDWWDANRHTVWEAITCKAEQNNKYFRDACST~~~GTPTHEKCRCVNRADVPTYFDYVPQYLRWFEEWAEDFCRKRNIKLKSAKNACRN~~~ESKQLYCSHNGYDCTKRIEKGKSMAL6P1.316 ~~~~~~~KNNDK~~~~~~~~~~~~~LDKISIEQVREYWWNANRDQVWEAITCHAAHSDEYFRKSTDG~~VTLYFDGRCGRELS~SVPTYLDYVPQFLRWFDEWSEEFCRKRNITLKSAKEECQN~~DSK~KLYCSLNGYNCTRLIPNKNYPF08_0140 NLNDKIKSN~~~~~~~~~~~~~YNDSEG~NYFKLREDWWTANRDQVWKAITCNAPKDANYFEYNSGK~~FFKFSNDQCGHNNGGAPPTNLDYVPQFVRWFEEWADDFCRIRKHKLKNIKDVCRN~~~ESKPLYCSLNGYDCTKTSWKNDFPFCLINvar30ELTSTNGKNGKKQ~~~~~ALQALYNDDNKNFFKLREDWWDLNRHDVWKAITCDAPDNAKYKVIKPDG~~STKDSTWKKCRNFA~DVPTNFDYVPQYLRWFEEWAEDFCRKKNKKLEDVIEKCRG~~KTKGEKYCSLNGYDCTKRIEKGKSIT4var19 DVMKTSG~SNGKTNGEIEARYGSDKDPN~~YYELREDWWDANRREVWKAMTCNADNDSQYFRQTCGSREKGSATKGKCRCV~IGDVPTYFDYVPQYLRWFQEWAEDFCRIKKIKLELAKKACRD~~~ESSKLYCSHNGYDCTKRIEKGKSIT4var32b DVMKTSG~SNGKTNGEIEARYGSDKDPN~~YYELREDWWDANRREVWKAMTCNADNDSQYFRQTCGSREKGSATKGKCRCV~IGDVPTYFDYVPQYLRWFQEWAEDFCRIKKIKLELAKKACRD~~~ESSKLYCSHNGYDCTKRIEKGKSDD2var47 DVMKTSGRTNGKKSAEAQKRYN~DATGN~~YYKLREDWWNANRDQVWKAITCDAADNDEYFENSSDG~~LYVFSNGQCGRN~EGKVPTNLDYVPQHLRWFDEWAEDFCRKRNITLKSAKEKCGG~~~EGNTKYCSLNGYDCTKVFEKKDSighvar19 ELREEQTKRKRAKPKNGQALQARYKKDGDNFFKLREDWWYANRQQVWKAITCHAGKDDAYFRNSSNR~~VYLFTDGQCGR~DETDVPTNLDYVPQFLRWFEEWAEEFCRLKKIKLELAKKACRK~~~DSQGLYCSHNGYDCTKPIEKESSraj116var11ELTTTSGRNKRTNGKKSAEAKKHYGSDE~NFFQLREDWWALNRRYVWDALTCNAPKDANYFEYNSGK~~LFKFSNDQCGHNNGGAPPTNLDYVPQFLRWFDEWSEDFCRKRKHKLKNIKDVCRN~~~ESKPLYCSLNGFDCTETVRRKDIraj116var08KLD~~~GKNGKKE~~~~~EAKDHYEDKDGNYFKLREDWWDANRHTVWKAITCGVTDGDKYFR~~~DT~~CSKGGSYKNCRCINGDVLTNFDYVPQFLRWFEEWAEEFCRIKKIKLKLAKNACRN~~ESK~QLYCSHNGYDCTKRIEKGDS

460 470 480 490 500 510 520 530 540 550 560 570 580 590 600. . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . |

1965-1 CSRDSKCTTCSNKCISYDLWLRNRRDEFKMQKGKYENEIKTKPSNTVISNSNINNEYHKTFYQNFGKNNHKTANYFLQLLNNGMYCKGGLPGEKDINFNETGDKDAFDRSKYCQPCPDCVVKCEGGKCEEEKKSDGKCMKEQIYTRPKDV1965-3 CSRDSKCTTCSNKCISYDLWLRNRRDEFKMQKGKYENEIKTKPSNTVISNSNINNEYHKTFYQNFGKNNHKTANYFLQLLNNGMYCKGGLPGEKDIDFNNTGGKGIFYRSEYCQVCPACVVKCDGGKCEEEKKSDGKCMKEQIYTRPKDV1983-1_ CSRESNCTACSNKCVDYDFWLEKQQNEFNMQKDKYDKEIRTKTSNNGISNSNINNKYYKEFYDELKKEYGSVEN~FLKLLNNGRYCQEGVKGESPINFNGTGNKHAFDRSEYCQPCPDCVVVCEGGECKE~NKGDDHCRSKIIEGIVRSE1983-3 CSRESNCTGCSNKCVDYDFWLEKQQNEFNMQKDKYDKEINGNNSLQNNKNNSIDKKYHYEFYKNFRKKGYNSLDKFLKLLNNGRYCKEGVKGESPIDFTITGDKDAFYRSKYCQPCPDCVVYCEGGECKE~NKGDDHCRSKIIEKIRQGEIT4var20 CSSDGNCTACSNICIAYDAWLRNQRNEFEKQKIKYTKEIEKYKSSSDKSNSNISNKYYNEFYENFGKKEYETLQNFLKLLNKGMYCQEKIEEEEVIDFNKDED~MVFHRSEYCQPCPDCVVQCKGGKCTE~DKKNDKCRSKIIKKILQSEIT4var06 CSRDSKCTECSTKCIPYEYWLEKQQNEFNMQKDKYDKEIRTKTSNNGISNSNINNKYYKEFYNELNKK~YESVENFLTLLNNGMYCKKRVEGKYVINFTKTADKDAFDRSEYCQPCPDCVVQCDGKRCAE~DTKDNNCRSKIIEEILQSEMAL6P1.316 CSRDPICTPCSNKCIPYDLWLRNRRDEFNMQKGKYENEIKTYESDNDISNSNINTEYYKSFYKKFAKSDYKKVHDFLTLLNNGRYCKEGVDGKDAIDFNKTDDKDAFDRSEYCQPCPACVVECDGGKCEEKKNSDGTCIEAQIYTVVRDEPF08_0140 ESREHYCTPCFSACSLYKIWVANQKKEFEKQKKKYEKEIPKYVSKKVKTNSTINNYYYYDFYEKLQKNEYGNIDAFLNLLKEGKYCKENLPEEEVINFTKHDEKKTFSLSNYCVVCPDCGVEYKNGRYTAKDQKYPDCRNEK~YDP~NNAPFCLINvar30CSREFKCTACLNKCIPYENWLEEQRDEFEKQKEKYKNERETYVNKNGISNSNINTEYYKTFYEKYAQNDYETVQGFLTLLNNGRYCKEGVKGESPINFNETDDKDAFYRSEYCQPCPDCVVKCDGKTCEQKKD~DENCRSKIIQKILQGEIT4var19 CSRDSKCTECSTKCIPYEYWLEKQQNEFNMQKDKYDKEIRTKTSNNGISNSNINNKYYKEFYNELNKK~YESVENFLTLLNNGMYCKKHVDGESTINFNKTDDKGAFYRSDYCQPCPDCVVVCEGGNCKE~KTEDDNCRSEIIKKILKYEIT4var32b CSRDSKCTECSTKCIPYEYWLEKQQNEFNMQKDKYDKEIRTKTSNNGISNSNINNKYYKEFYNELNKK~YESVENFLTLLNNGMYCKKHVDGESTINFNKTDDKGAFYRSDYCQPCPDCVVVCEGGNCKE~KTEDDNCRSEIIKKILKYEDD2var47 CSSDGNCTACSNICIAYDAWLRNQRNEFEKQKTKYTKEIEKYKSSSDKSNSNISNKYYNEFYENFGKKEYETLQNFLKLLNKGMYCQEKIEEEEVIDFNKDED~MVFHRSEYCQPCPDCVVQCKGGKCTE~DKKNDKCRSKIIKKILQSEighvar19 CSRESKCTECSTKCIPYEYWLEKQQKEFEKQKEKYENEIKTYESDNDISNSNINNKYYKEFYEKFKDKGYNSLNEFLKLLNNGSYCKG~VDGKDAIDFTNNVEK~TFDRSKYCQPCPDCVVVCSNGTCSQ~KKDDDHCRSEIIKKILKYEraj116var11FIESNDCMKCFSACSHYSIWVDNQKKEFEKQKKKYEKEIPKYVPKKDKRLSNINNEYYNDFYKNLKEMKYDNINTFLKFLNEGKYCKENLPEEEVINFIIPDEKKTFHRSKYCEPCPICGVKCENKSCTEKENDD~DCKNKKKYDPPKGVraj116var08CSRTEKCTECSTKCIPYEYWLEKQQNEFNMQKDKYENEIKTYVNNNGIPNSNINNEYYEIFYKQLIEKKYENYNDFLKLLNIGKYCIG~LKGEEDIDFTNIGEKGTFYRSDYCQPCPDCVVDCTGGNCKENKK~HDNCRSKIIEEILRSE

610 620 630 640 650 660 670 680 690 700 710 720 730 740 750. . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . |

1965-1 TPKKIKVLFSGDNQEDITEKLSSFCKNPESENNTEYQTWQCYYKKRDHNNCEMKGSSYKEKHDPNIIISDECFHLWVKNLLIDTIKWETKLKKCINNTNVTDCDNECNNNCECFDKWIDTKK~EWEEVRKVYKDQETILGVYFKNLNNIF1965-3 TPKKIKVLFSGDNQEDITEKLSSFCRKPKDVNDANYKTWQCYYNDSDDNNCEMKGSSYKDKHDPNIIVSHECFHLWVKSLLIDTIKWETKLKNCIDYTNVTDCDNECNNNCECFENWVEQKK~EWENVNDVYKNQKGTLDIYYKNVKNLF1983-1_ TPTVIDVLYSGKGQGLITEKLKDFCSNPNNDKGENVQTWKCYNKNSEYNKCEMISWLYQDPKEYNLMLSVECFHSWAKNLLIDTIKWEHQLKNCINNTNVTDCTSKCIKNCECYEAWINRKKDEWKKLKEVLNKKDQTSHNYYNKLKDVF1983-3 KPIEIEVLYSGKGQGLITEKLSSFCSNPENKNDTNYQNWKCYNKNSEYNKCEMISWLYQDPKEYNLMLSVECFHSWAKNLLIDTIRWEHQLKNCINNTNVTDCTSKCIKNCECYEAWINRKKDEWKKLKEVLNKKDKNSDNYYNKLKDVFIT4var20 EPTEIHVLNSDDKQGDITKKLEVFCSSTTNYEGRNVQKWKCYNKNSDYNNCEMNISSYKDSTDANVMLSVECFHSWAKNLLIDTIKWEHQLKNCINNTNVTYCESKCIKNCECYEKWIKRKEHEWEKVKNVFGNNNRMSYIYYNNLSRVFIT4var06 ETTDIYVLYSGKGPGAITKKLHDFCSNTNKEDRKYYKKWKCYNKNKDYNNCEMNISSYKDATDPNVMLSVECFHSWAKNLLIDTIRWEHQLKNCINNTNVTDCKSKCNSNCQCYEKWIKEKEKEWPQVKGVLKKKDETSHNYYDKLKDVFMAL6P1.316 TPTPIKVLFSGDHQKDITKKLSSFCKNPESENNRDYQTWQCYYKSSDYNNCEMKGSLYKVEGDPNIIVSHECFHLWVQSLLIDTIKWETKLKKCINNTNVTNCYNKCNKNCECFENWVEQKKKEWENVNDVYKDQKQSLGIYYEKLENLFPF08_0140 ETTDITVLYSGD~VGDFSEKLQDFCNDINNGKVKNYQIWQCYYENSEINKCQMTPSSHKVPKHGYIMSFFAFFDLWVKNLLIDTINWKNELTNCINNTNVTDCKNDCNTNCKCFENWAKTKEKEWENVKTIYKNENRNTNNYYKKLNDLFPFCLINvar30EPTVIDVLYSGKGQGLITKKLHDFCSSTNKEDDKYYKKWKCYNKNSDYNNCELISSLSTDPTDPKVMLSIKCFDSWARNLLVDALKWEHQLKNCINNTNVTDCKSNCNNNCKCYEEWIKQKEKEWQKVKGVLKKKDKNSDNYYKNVKNLFIT4var19 TPTPIDVLYSGKGQGLITKKLEDFCSSTTNYEGTNVQKWKCYNKNKDYNNCEMNISSYKDATDPNVMLSVECFHSWAKNLLIDTIRWEHQLKNCINNTNVTDCTSKCIKNCECYEKWIKEKEKEWPQVKGVLKKKDETSHNYYDKLKDVFIT4var32b TPTPIDVLYSGKGQGLITKKLEDFCSSTTNYEGTNVQKWKCYNKNKDYNNCEMNISSYKDATDPNVMLSVECFHSWAKNLLIDTIRWEHQLKNCINNTNVTDCTSKCIKNCECYEKWIKEKEKEWPQVKGVLKKKDETSHNYYDKLKDVFDD2var47 EPTEIHVLNSDDKQGDITKKLEVFCSSTTNYEGRNVQKWKCYNKNSDYNNCEMNISSYKDSTDANVMLSVECFHSWAKNLLIDTIKWEHQLKNCINNTNVTYCESKCIKNCECYEKWIKRKEHEWEKVKNVFGNNNRMSYIYYNNLSRVFighvar19 TPTPIEVLYSGDGQGLITEKLHEFCRGPNKVDSKNYKTWNCYNKNNDYNKCEMISWLYEDPKKSNLMLSIQCFDSWVQNLLIDTIKWEYELKDCINNTYDADCNDECNKNCECYEKWIKQKEKEWQKVKNVFGNNNRMSYIYYNNLSRIFraj116var11TPIDIPILYSGDKQGDITKKLEDFCYNPTKENEKTYQNWKCYYKDSEFNKCKMESKSGKSTTQEKIISFDEFFYLWVNNLLIDSIMWENDIKHCINNTNVTNCNNGCNENCICFEKWVGQKEKEWENVKKVLKNPSKNLNNYYNKLNGIFraj116var08ETTDIDVLYSGKGPGAITKKLHDFCSNPNNYKRANVQKWKCYNKNKDYNNCEMNISSYKDATDPNVMLSVQCFYSWAQNLLIDIIRWEHQLKDCINNTNVTDCDNECNKNCECFEKWIKQKQKEWQQVKEVLKKKDENSHNYYNKLRSVF

760 770 780 790 800 810 820 830 840 850 860 870 880 890 900. . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . |

1965-1 DSYFFEVMIALDQDEK~GKWDQFRKDLEKNLNLPKN~TDTENSQDAIEFLLDHLKDNATICKDNNSLEPCTSPPNPTPNPCGK~~~~~~~~~KNNGGK~LVRVKRLAEMMQRYARKQLEKGA~GETKLKGDASKGEYKRGGTGDHFKN~L1965-3 DSYFFEVMIALDQDEK~GKWDQFRKDLEKKFGTSEKKTRTTDSQDAIEFLLDHLKDNATICKDNNSNEACDSSKNRTPNPCGK~~~~~~~~~KNNGGK~LVRVKRLAEMMQRYARKQLEKGA~GETKLKGDATKGTYRQGGEPSDFKN~V1983-1_ DRFLFQVMFALDQDEK~VKWDQFKDDLNKKFESSKDKASTANSQDAIEFLLDHLKDNAITCKDNNTNEACESFKESKTNPCGK~~~~~~~~~NPSASNNLVRVKRPAETMQRYARKQLEKRG~GESKLRGDATKGNYSRGGSGDDFKN~L1983-3 DRFLFQVMFALDQDEK~GKWDQFRKDLEKKFGPSVESAGTANSQDAIEFLLDHLKDNATTCKDNNSNEACDPSKKVKTNPCGK~~~~~~~~~KNNGGK~LVRVKRLAEMMQRYARKQLEKRG~GESNLKGDASKGKYKHGGPESDFKENLIT4var20 DSFLFQVMFALDQDEK~GKWDQFTEDLKKKFEPSKTNTPTGKSQDAIEFLLDHLKDNALTCRDNNSNESCDVSKKVKTNPCGK~~~~~~~~~NPSASNNLVRVKRLAEMMQRYARKQLEKRG~GEINLKGDATKGTYRQ~GGPADGFKNVIT4var06 DRFLFEVKGALDQDEK~GKWDQFTKDLEKKFGPSVESAGTANSQDAIELLLDHLNDNAITCKDNNSLKEDKNCPKTKINPCIKGTCKR~~~~TGGASNNLVSVKHIAEIMQQDAREQLEKRG~GESNLKGDASKGTYGQ~GGHGDVFKDVMAL6P1.316 KSNFFQVMKALEGDEK~GKWYQFKDDLKKKFEPSEKNTRTTDSQDAIKLILDHLKDNATTCKDNNSLEEDENCPKTKINPCIKRTRIP~~~~TRGASNNLVSVKHIAELMQRSARKQLEAGAG~EINLKGDATKGKYTKKNGKAVALNDIPF08_0140 KGYFFHVMYELNNEEA~~KWNKLMKNLRTKIDSSRKNAGNEDSEGAIKVLFDHLKDIAERCIDNNSIKPCNSHPKPTPNPCGK~~~~~~~~~~NNNGGKLVRVKRPAELKQQKAHIQLEKRG~GESNLKGDATRGTYNLGCQGDQLDATFPFCLINvar30YSFLFQVIYELEKEEKNGKWDQFMEDLKKKIEASQKNKGTENSQDAIELLLDHLKDNATICKDNNTNEACDPTGNPTQNPCVKNN~~~~~~~~~~NGGKVVSVKQIAQYYKRKAHAQLEEGGDSRSALKGDASQGTYGQGGEASDFKEKLIT4var19 DRFLFEVKGALDQDEK~GKWDQFTKDLEKKFGPSVESAGTANSQDAIELLLDHLNDNATTCKDNNSLAVEN~CPKTKINPCIKGTRKRTPKRTGGASNNLVSVKHIAEIMQQDAREQLEKRG~GESNLKGDASKGKYTKKNGKAVALNDIIT4var32b DRFLFEVKGALDQDEK~GKWDQFTKDLEKKFGPSVESAGTANSQDAIELLLDHLNDNATTCKDNNSLAVEN~CPKTKINPCIKGTRKRTPKRTGGASNNLVSVKHIAEIMQQDAREQLEKRG~GESNLKGDASKGKYTKKNGKAVALNDIDD2var47 DSFLFQVMFALDQDEK~GKWDQFTEDLKKKFEPSKTNTPTGKSQDAIEFLLDHLKDNALTCRDNNSNESCDVSKKVKTNPCGK~~~~~~~~~NPSASNNLVRVKRLAEMMQRYARKQLEKRG~GEINLKGDATKGTYRQ~GGPADGFKNVighvar19 DSFLFPVIYKLQKEEKDGKWDQFTKDLKKKFESSETNTPTGNSQDAIEFLLDHLKDNATICKDNNTNEACDSSKKETQNPCGK~~~~~~~~~NTDVSK~YTSVKQIAQYLKRKAYYEANKRSDGLHKLKGKAHEGKYNQKGTPSDLKENIraj116var11SGFFFEVVYKFNNKEE~~KWNQFTEDLKKKIEASQKNKGTENSQDAIELLLDHLKDNAITCKDNNSLKEDKNCPQTKTNPCIKGTRKP~~~~TRGASNNLVSVKHIAELMQQKAHIQLEKRG~GEYNLKGDATRGTYKRGGSGDHFKN~Lraj116var08DSFLYQVMFALNNEEK~GKWDQFTEDLEKKFESSKKSAGTGNSQDAIEFLLDHLNDNAITCKDNNSKESCENSKGSKPNPCIKNP~~~~~~~~~SASNNLVSVKHIAEIMQQDAREQLEKRGG~ESKLKGDATKGEYKRGGQKKNLNGHI

910 920 930 940 950 960 970 980 990 1000 1010 1020 1030 1040 1050. . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . |

1965-1 CSITKIDS~~~NDIRSN~~GEPCQGKNKE~~~~~RFKIGTQWSFKDDNKKNTHPEAYMPPRREHMCTSNLEKID~~VKSVI~KNGN~~~~~~~~AIHSLLGDVLLAAKYEAEKIKELYQQNN~~~SKNDLNDSNDQATICRAMKYSFSDI1965-3 CSISEKHTNVQDNNRAYTYKGPCTGKNQG~~~~~RFEIGKEWKD~GREIQFPIND~YMPPRRQHMCTSNLENLNIMSEGLN~NSSL~~~~~~~~VSNSLLGDVLLAAKEEAKNIKELYEKNK~~~GTRGLNDENDKATLCRAVRYSFADL1983-1_ CSITNKDS~~~NDSRSNTTDGPCTGKNPG~~~~~RFEIGTKWET~GPNVEMTETEAYMPPRRQHICTSNLEKLN~~AKCVT~SSSN~~~~~~~~VNGSFLGDVQLAAKYEAENIKKLYNRYN~~~SKNDLNDSNDKETICRAMKYSFADI1983-3 CKIDARHS~~~~NRNQVYSDQPCYGKGTGTGINTRFEIGTVWQPDPQHMRVGHEDVIMPPRRRHMCTSNLEFLQTKDSPLN~SDDGK~~~~~~LVNNSFLGDVLLSAKYEAQNIIDMYKDKNKLSSPNDLTNPKHQETICRAIRYSFADLIT4var20 CSINQNHTNVQNNNRAYTYQGPCTGKDGSN~GGVRMKIGTPWKPG~RQIQMSAEDIYMPPRRQHMCTSNLEYLQTKDGPLKQGD~~~~~~~GKLVNNSFLGDVLLSAKMDAGKIIELYKKQN~~~NKSNLTDPEDNESACRALRYSFADLIT4var06 CNITASHSNATTT~~~~FSKGPCYGKDKVI~NGVRMKIGNRWTHLQNAK~TTYSDVFLPPRRQHMCTSNLEYLQTKDGPLNGMEIVKNGKNGKLVNNSFLGDVPLSAKMDAGKIIELYKKQN~~~NKSNLTDPEDNESACRALRYSFADIMAL6P1.316 CSIDVQHSN~STYRS~~~~EKPCAGKNTG~~~~~RFDIGTPWKT~GTNVKMTEDQAYMPPRREHMCTSNLEYLETDQGPLKNSDGKF~~~~~~~VNHSFLGDVLLAANHEAKKIKELYTKDN~~~GLND~~~LKDKETVCRAMKYSFADLPF08_0140 CNIDEKYS~~~NRIPR~KSEGPCYGKNPQ~~~~~RFYTGKDWTH~VVQEKTSYKDVFLPPRREHMCTSNLENLNLNSKGLSNSSIA~~~~~~~~~SNSLLGDVLLAAKYEADDIVKK~~~~~~~~~~~~~NDS~DKSSICNAMKYSFADIPFCLINvar30CDINEKHSN~DSRRRRGDYNGPCTGKNDK~~~~~RFKIGTDWID~ANFVSTIHKDFYLPPRREHMCTSNLEKLHVGSVTKNDK~~~~~~~~~~~AIHSLLGDVLLAAKYEAEFIKTNYTRLN~~~GQND~~~NGAK~~~CRAMKYSFADIIT4var19 CSIDVQHSNSTYR~~~~SVK~PCAGK~~~~~NTGRFDIGTPWSYGEKKKEMTHPKVYMPPRREHMCTSNLEYLETGEGPLKGKD~~~~~NNGKLVNNSFLGDVLLSAKKEGDFIVKKLG~~~~~~~~~~~~~~SDKSAICNAMKYSFADLIT4var32b CSIDVQHSNSTYR~~~~SVK~PCAGK~~~~~NTGRFDIGTPWSYGEKKKEMTHPKVYMPPRREHMCTSNLEYLETGEGPLKGKD~~~~~NNGKLVNNSFLGDVLLSAKKEGDFIVKKLG~~~~~~~~~~~~~~SDKSAICNAMKYSFADLDD2var47 CSINQNHTNVQNNNRAYTYQGPCTGKDGSN~GGVRMKIGTPWKPG~RQIQMSAEDIYMPPRRQHMCTSNLEYLQTKDGPLKQGD~~~~~~~GKLVNNSFLGDVLLSAKMDAGKIIELYKKQN~~~NKSNLTDPEDNESACRALRYSFADLighvar19 CNITLEHS~~~~NRDPKRSRGPCHGKDGD~~~NKRFKIGTQWKG~GEQVNKTYKDVYLPPRREHMCTSNLEFLQTTDNPLNGSEGGN~~~~~~KVNDSFLGEILLTAYREAERTKDYFKHEK~~~~~~~~~~~~DNPVACRAIRYSFADIraj116var11CSITKIVS~~~NDSRNGNNGGPCTGKNPE~~~~~RFNIGTEWKQ~GETLGTN~VEIYMPPRREHMCTSNLEKIDVKS~~VIKNGNA~~~~~~~~~SHSLLGDVLLAAKYEAKNIKELYQQNN~~~SKNDLNDSNDQATVCRAMKYSFADIraj116var08CNIDKNYTN~DTRSYGGPCHGKDGNQGGD~~~~~RMKIGTPWKG~GRDVKTSYEDVYLPPRREHMCTSNLEKLHVESVIKNGD~~~~~~~~~~~ASHSLLGDVLLAAKMDAAEIINRYKSQN~~~SIGDPIDKKHQESICRALRYSFADL

1060 1070 1080 1090 1100 1110 1120 1130 1140 1150 1160 1170 1180 1190 1200. . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . |

1965-1 GDIIRGKDMWVQNYGENTTQGNLKDIFGTIKNKNP~EIQGKYKH~~~IDDEKHTQLRSDWWEANRHQVWRAMKCAIEKDKNLKCNG~~~~~~~~~~~IPIEDYIPQRLRWMTEWAEWYCKEQSLLYGELVTKCAGCISK~~GGQCTQKDN1965-3 GDIIRGRDMWEHK~DQNKLQGHLKTIFDKIKEKLSDDIKGKYT~~~~~DGSPYLDLRKDWWEANRHQVWRAMKCATKAIPDMKCNG~~~~~~~~~~~IPIEDYIPQRLRWMTEWAEWYCKVQKEAYGELLEKCDNCKNKEDGKGCMQNTQ1983-1_ GDIIRGRDLWDINSDAKKLQTNLKEIFTKIKEELPKEIQGKYT~~~~~DGEPYIKLREDWWEANRHQVWRAMKCATKAIPDMKCNG~~~~~~~~~~~IPIEDYIPQRLRWMTEWAEWYCKYQSQEYKTLQDKCNQCKNKGDGK~CTQGNG1983-3 GDIIRGRDIWDKENGMVKLRGYLQTIFGKIQEKVP~~~~GKYD~~~~~GDTDHKLLRSDWWEANRHQVWRAMKCATKKDNIETCKG~~~~~~~~~~~IPIEDYIPQRLRWMTEFAEWYCKVQKEEYDKLVTGCTQCKFQG~~~~CTSDDDIT4var20 GDIIRGRDLWDKNSDAKRLQTNLKEIFTKIKEELP~~~EDIKKKYDKDGTD~HKLLREDWWEANRHQVWRAMKCAIENDKDMKCNG~~~~~~~~~~~IPIEDYIPQRLRWMTEWAEWFCKEQSRLYNKLVADCKSCKGKA~~KSCTQKDGIT4var06 ADIIRGRDMWDKDDGAQKMDVILKNVFGTLHKSLEGIRNHPKYAYDKNKTSPYKQLREDWWEANRHQVWRAMKCATKAIPDMKCNG~~~~~~~~~~~IPIEDYIPQRLRWMTEWAEWFCKMQSQEYEKLEKECGGCKEMG~~GQCTNDTEMAL6P1.316 GDIIRGRDMWDNETGMKHAKKHLKDVFDNIRKSLK~NKGNQKYNYDDKKLPPYKELREDWWEANRHQVWRAMKCAIKEATIDNCNG~~~~~~~~~~~IPIEDYIPQRLRWMTEWAEWYCKMQSQEYKKLQDACTGCKKKV~~DSCTKGTPPF08_0140 GDIIRGKDMWDLDSGSKDMEKHLISIFEKIKENLD~~VIKYNSKY~~KDTQKFLDLRSDWWEANRHQVWRAMKCAIENDKDMKCNG~~~~~~~~~~~IPIEDYIPQRLRWMTEWAEWYCKAQKKEYDELVKGCKGCMGNG~QGCTKDSSDPFCLINvar30GDIIRGKDMWDKNKDATGLQSRLENIFKNINEKLP~DGIRKKYN~DGKHLN~~~~LRSDWWEANRHQVWNAMKCPIKEFNDTSVTTQSNGYCGYSDHTPLDDYIPQRLRWMTEWAEWFCKAQAEAYEKVQHACANCNN~~~~GVCKN~~~IT4var19 GDIIRGRDMWDKDSGSKKMDVILKNVFGTLHKSLEGIRNHPKYAYDKNKTSPYKQLREDWWEANRHQVWRAMKCEIKKDKNMKCNG~~~~~~~~~~~IPIEDYIPQRLRWMTEWAEWYCKAQNKYYGELEKECGGCMGNG~~QGCTSSDPIT4var32b GDIIRGRDMWDKDSGSKKMDVILKNVFGTLHKSLEGIRNHPKYAYDKNKTSPYKQLREDWWEANRHQVWRAMKCEIKKDKNMKCNG~~~~~~~~~~~IPIEDYIPQRLRWMTEWAEWYCKAQNKYYGELEKECGGCMGNG~~QGCTSSDPDD2var47 GDIIRGRDLWDKNSDAKRLQTNLKEIFTKIKEELP~~~EDIKKKYDKDGTD~HKLLREDWWEANRHQVWRAMKCAIENDKDMKCNG~~~~~~~~~~~IPIEDYIPQRLRWMTEWAEWFCKEQSRLYNKLVADCKSCKGKA~~KSCTQKDGighvar19 GDIIRGKDLWERNRDMLKLQGHLKKIFGHINKSLKKTLNGNTKYE~~KDGEPYTKLREDWWEANRHQVWRAMKCHIEDLKDPSIDRSKS~HCGYSDHTPLDDYIPQRLRWMTEWAEWFCKEQSQEYDKLEKECEECRSKGDGKGCTKESGraj116var11GDIIRGKDMWVENMDARKLQAYLAKIFEEIKDKIP~~DKGTKDKY~~EKHSNLIKLGEDWWEANRHQVWRAMKCATKAIPDMKCNG~~~~~~~~~~~IPIEDYIPQRLRWMTEWAEWFCKEQSKLYGELVRDCASCKKKGKEKCTQ~~GDraj116var08GDIIKGTDMWEHS~DQTKLQGHLKKIFGKIKQEPP~QNIKDKYNGDDNNTPPYKKLREDWWEANRHQVWRAMKCTTKNINNNKCNG~~~~~~~~~~~IPIEDYIPQRLRWMVEWAEWFCKEQSRLYEELVEACGSCKGKV~~QGCTKDSS

1210 1220 1230 1240 1250 1260 1270 1280 1290 1300 1310 1320 1330 1340 1350. . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . |

1965-1 ~~DCEPCKAACAKYTENIKKWEPQWKKIKKKYKILYDKATK~~~~~~~HAVNTSNDPKDEKDVVDFLKHLVPRKS~~~~~~~~~~~~~~~~~~~~~~~~~~~KNT~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~PGVATTAPITLYSSA1965-3 ~~ECEKCTQACKDYNSKIEPWREQWKKIKGKYEELYKKALDSVNGNGKGEKSTTSGPKDEKDVVVFLKQLLPRNS~~~~~~~~~~~~~~~~~~~~~~~~~~~AAARVLVKHAAGN~~~~~~~~~~~~~~~~~~SVTRVTALTPITLYSSA1983-1_ ~~ECKTCTEACEAYRDKIKKWENQWKKIKGKYKKLYEQATK~~~~~~NGETSGTASDRKDKDVVVFLKQLLPRNS~~~~~~~~~~~~~~~~~~~~~~~~~~~AAARNRVIRAAP~~~~~~~~~~~~~~~~~~~GPTRVTATTPITLYSSA1983-3 ~~KCEMCTNACKKYGENIKTWENQWKKIKDKYKTLYDKATK~~~~~~NGETSGTPNEK~DKDVVVFLKQLLPRNS~~~~~~~~~~~~~~~~~~~~~~~~~~~AAARVRVKRADGS~~~~~~~~~~~~~~~~~~SATRVTATTPNTLYSSAIT4var20 ~~DCTKCKAACDNYNKKIKPWEEQWEKIKNKYAQLYKKALDSVNGKEESKKKTASDAKD~QQVVHFLAELIRKSG~~~~~~~~~~~~~~~~~~~~~~~~~~~GGKGGKNVKTTVS~~~~~~~~~~~~~~~~~~~~~~~PTTTPNTLYSSAIT4var06 ~~DCQRCTQACEEYKKKIEPWEKQWTKIKDKYKTLYEQATK~~~~~~NGETSGTPNEKD~KDVVDFLKQLLAQNS~~~~~~~~~~~~~~~~~~~~~~~~~~~AAARNRVIRAAGNS~~~~~~~~~~~~~~~~~ATRVIATTAPNTPYATAMAL6P1.316 ~~DCEQCDKQCKQYTEFITKWQPQWETMSYKYQTLYEEAERDA~~TSGSVKKRTQLSKEDQRVVDFLKQLLLRNSA~~~~~~~~~~~~~~~~~~~~~~~~~~~AARNRVIRAAGSS~~~~~~~~~~~~~~~~~ATGGTTAMTPNTPYSTAPF08_0140 G~ECKKCTEACNTYKQKIKTWENQWKKIKGKYEKLYEQATK~~~~~~NGETSGTPNEKD~KDVVDFLKQLLPRNS~~~~~~~~~~~~~~~~~~~~~~~~~~~AAARNRVIRAAG~~~~~~~~~~~~~~~~~~SSAPGVTALTPITLYSSAPFCLINvar30~~ECDNCKTKCEDYRKFIEKWKKQWDEQEKNYQELYEKATQNG~~SDGSKVT~~~~ADKDADVVDFLSKLRNKN~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~DTNNLFESAIT4var19 ~~KCKQCKNACDAYKKKIETWEKQWEKIKGKYEELYKKALDSVNGNGKGEKSTTSGTKDEKDVVDFLKQLLAQNS~~~~~~~~~~~~~~~~~~~~~~~~~~~AAARVRVIRAAG~~~~~~~~~~~~~~~~~~~SPTEITAAAPITPYSTAIT4var32b ~~KCKQCKNACDAYKKKIETWEKQWEKIKGKYEELYKKALDSVNGNGKGEKSTTSGTKDEKDVVDFLKQLLAQNS~~~~~~~~~~~~~~~~~~~~~~~~~~~AAARVRVIRAAG~~~~~~~~~~~~~~~~~~~SPTEITAAAPITPYSTADD2var47 ~~DCTKCKAACDNYNKKIKPWEEQWEKIKNKYAQLYKKALDSVNGKEESKKKTASDAKD~QQVVHFLAELIRKSG~~~~~~~~~~~~~~~~~~~~~~~~~~~GGKGGKNVKTTVS~~~~~~~~~~~~~~~~~~~~~~~PTTTPNTLYSSAighvar19 R~TCTKCTEACKEYKTKIQPWKDQWKKIKNKYEELYLQAHITAIN~GGPEYFGRSVDTKDKPVVNFLFELYKANGGRISFPRGTKAVVIALRNKVVVIALAKAPAKASAKASVTR~~~~~~~~~~~~~~~~~~IKRSISTTTQKTPYSTAraj116var11N~DCTKCKAACDKYKEEIEKWNEQWKKIKAKYEQLYKKALDSVNGNGKGEKSTTSGTKDEKDVVDFLKQLLAQNS~~~~~~~~~~~~~~~~~~~~~~~~~~~AAARNRVKRAAALVCFKRAATRVCVKRAASSSATRVTATTPNTPYSTAraj116var08DGECTKCTQACNTYKAKIKKWEDQWKEIKEKYDDLYKKSLENGDTVTSGSPKVTIISKEDERVVEFLKNLHDGNK~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~DMSNIYSSA

1360 1370 1380 1390 1400 1410 1420 1430 1440 1450 1460 1470 1480 1490 1500. . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . |

1965-1 AGYIHHELGRTVGCMKQDVFCL~VRN~~~~~~~~~NYAFKEPPDGYGEACTCKER~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~DAPSPKLPKEEKKD~~~~~~~~~~~~~~~~~~~ACDIVHEILNG~EDGKNKIDG1965-3 AGYIHHELGRTVGCNTQTRFCA~EKKP~~~~~~~~GYAFKHPPKEYEDACGCNER~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~NTKPPAPEPKKQKG~~~~~~~~~~~~~~~~~~~ACEIVDGILNG~KNGKTSING1983-1_ AGYIHQEL~QQVGCNTQTRFCA~EKRP~~~~~~~~GYAFKHPPKGYEEACGCDKN~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~VLKPPE~KKEEIKK~~~~~~~~~~~~~~~~~~~ACEIVDGILNG~KNGKTSING1983-3 AGYIHQEL~PHMQCKGQTRFCA~EKQP~~~~~~~~EYAFKEPPDGYDEACKCDKN~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~LPKPPE~KKEEIKK~~~~~~~~~~~~~~~~~~~ACEIVENLIRK~NDGNDLNGMIT4var20 AGYIHHELGRTVGCNTQKEFCYSKNG~~~~~~~~~KYAFKDPPKGYEEACKCN~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~DRNPKPQPAPKKEDE~~~~~~~~~~~~~~~~~~DACDVVKPLLKD~KGETDDIDGIT4var06 AGYIHQELQN~MECQVQKEFCEKKRG~~~~~~DKKKYAFRHQPYDYDDALRC~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~DSRTAPEPKKVEKKE~~~~~~~~~~~~~~~~VLDVCGVVKGVLSKPRNKKGGIDGMAL6P1.316 AGYIHHELGKTVGCNVQTKFCKHKIGSKASGTENKEYAFREKPYDHDDACACRPPKPTGGPGAGESPARSDADSRGPATVGAGEVEEDGDEVDSEEEEDEVEEEPPAEE~~~~~~~~~~~~~~~~~~~~VCEMVDTLLDESNGGKNGINGPF08_0140 AGYIHQELGKTVGCNTQKEFCKNGSGE~~~~~~~~KYAFKHPPKEYKDACSCNTR~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~DKKSEAPVTKKEE~~~~~~~~~~~~~~~~~~~~ACKIVDGILKG~NDGINEISGPFCLINvar30AAYVH~DTGNLDDCKEQKYFCQK~~~~~~~~NDSNNYAFREKPYLYDKACNCQEPPKS~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~KKKVE~~~~~~~~~~~~~~~~~~~~TWEIVKEVLLKSKNSRGGIDGIT4var19 AGYIHQELGKTVGCNIQTKFCKHKIGSKASGTENKEYAFRDKPHDHDEACACRPPSTPVDVSRALKPRGPA~~~~~~~~~~~~~~~~~~~~~~SEDSEDDEDEDEVEEEEGTTPGPPATPVPAGPTVVDVCKIVDGILKG~NDGSNEISGIT4var32b AGYIHQELGKTVGCNIQTKFCKHKIGSKASGTENKEYAFRDKPHDHDEACACRPPSTPVDVSRALKPRGPA~~~~~~~~~~~~~~~~~~~~~~SEDSEDDEDEDEVEEEEGTTPGPPATPVPAGPTVVDVCKIVDGILKG~NDGSNEISGDD2var47 AGYIHHELGRTVGCNTQKEFCYSKNG~~~~~~~~~KYAFKDPPKGYEEACKCN~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~DRNPKPQPAPKKEDE~~~~~~~~~~~~~~~~~~DACDVVKPLLKD~KGETDDIDGighvar19 AGYIHQELGKTVGCKEQTKFCI~GGE~~~~~~~~~KYAFKEPPE~YATACDCINRS~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~QTEEPKKKEENVES~~~~~~~~~~~~~~~~~~~ACKIVDGILKG~DHGNRKVGDraj116var11AGYIHQELTPHMQCKGQTRFCG~ETHP~~~~~~~~EYAFKHPPKEYKDACSCNTR~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~DKTSEAPVTKKEED~~~~~~~~~~~~~~~~~~~ACEIVKELLKD~KKATDDIDGraj116var08EAYVH~ETADVSDCNTQNVFCNT~~~~~~~~TGKENYAFQEIPKVYEQACDCKK~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~WTKKTD~~~~~~~~~~~~~~~~~~~~ACTIATNLVKDNDGEKK~ING

1510 1520 1530 1540 1550 1560 1570 1580 1590 1600 1610 1620 1630 1640 1650. . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . |

1965-1 CNPKN~~~~~~YKGWKCKASDVNSDHVGACMPPRRQKLCVSGLTKPYR~~~IEAIEHIRTEFIKSAAIETYFAWLKYKEINTG~~ADNELQTGNIPEGFIRQMYYTFGDYRDIFFGTDISKHSHISRVSSSVKHILK~KESKEREE~~~~1965-3 CRTKTN~~~VTYPPWDCTKTNVNAEHNGACMPPRRRKLCLHYLTKSNN~~~LNSEDHIRNNFITCAAIETYFAWDRYKTKNQG~~AVDQLKNGKIPDEFKRQMYYTFGDYRDIFFGTDITSHNHIPEVSSNVITILQ~RANGTKSEDKQK1983-1_ CGTKTN~~~VTYPPWDCTKTNVNAEHNGACMPPRRRKLCLHYLTKLYN~~~LKSKEDIRNNFITCAAIETYFAWDRYKTKNQG~~AVDQLKNGKIPDEFKRQMYYTFGDYRDIFFGTDITSHNHIPEVSSNVITILQ~RANGTKSEDKQK1983-3 CNPKN~~~~~~YNGWKCKASNVHSDHVGACMPPRRQKLCIYFLANPTQKPYINTKVNLRDAFIKSAAAETFRSWHHYKSKNSDEKLQKQLESGEIPEDFKRQMFYTFGDYRDLCLGKDIGNDSGK~DISGTVTTILN~SDKVSETKTTP~IT4var20 CNQKYKAGKDKYPGWDCNSQIHTT~HNGACMPPRRQKLCVSGLTKTDR~~~IKAIEYIRTEFIKSAAIETHFAWDRYKEDNGE~~AEAELKNGNIPEGFKRQMYYTFGDYRDIFFGRDISTHAYISGVSPKVITILE~KENDAKYAAKQNIT4var06 CNP~~~~~KKDYPSWHCNKKESMAENSGACIPPRRRKLCVSSLTKQYR~~~IKAIEDIRTEFINCAAIETYFAWERYKKDNKK~~AEDELKSGTIPHEFKRQMYYTFGDYRDIFFGTDISTHAHISDVSPNVITILERKENGTKSEGKQKMAL6P1.316 CNPKDQ~~VQPYPGWDCKLSTFKDKEHGSCMPPRRIKLCVSGLTQTNN~~IINKED~IRTHFITCAAIETYFAWLRYKKINTE~~ADKELKEGKIPDEFKRQMFYTFGDYRDIFFGTDISTHNHIPEVSSKVITILEKENG~TKSEDKQKPF08_0140 CNPKE~~~~~SYPEWQCDKSKFKNGEEGACMPPRRQKLCVSGLTQGGK~~~ITTIEDIRTQFITCAAIETHFAWHKYKKDNVN~~AERELNSGIIPHEFKRQMYYTFGDYRDIFFGTDISTHNHIPEVSQKVITILE~KENLTKSEVKQKPFCLINvar30CNPKT~~~DEPYPTWDCEKNESNVKNKGACIPPRRQKFCTSLLTKKDV~~FKTNEENIRTEFINSAAIETYFAWERYKQDNKK~~AEAELINGRIPETFKRQMYYTFADYRNIFFGTDITSHDHILDVSKNAKERLISEIGKQKTSDTENIT4var19 CNP~~~~~KESYPDWDCKKNIDNS~HSGACMPPRRQKLCVRDLTQGGE~~~IRKPEDILTKFINCAAKETHFAWHKYKKDNVN~~AENELKSGKIPEGFRKQMYYTFGDFRDIFFGTDISSCRYIKDTSQTIKSKLGDQATTEKGD~THIIT4var32b CNP~~~~~KESYPDWDCKKNIDNS~HSGACMPPRRQKLCVRDLTQGGE~~~IRKPEDILTKFINCAAKETHFAWHKYKKDNVN~~AENELKSGKIPEGFRKQMYYTFGDFRDIFFGTDISSCRYIKDTSQTIKSKLGDQATTEKGD~THIDD2var47 CNQKYKAGKDKYPGWDCNSQIHTT~HNGACMPPRRQKLCVSGLTKTDR~~~IKAIEYIRTEFIKSAAIETHFAWDRYKEDNGE~~AEAELKNGNIPEGFKRQMYYTFGDYRDIFFGRDISTHAYISGVSPKVITILE~KENDAKYAAKQNighvar19 CNPKTE~~~GTYPKWKCGNINLVKDPR~VCMPPRRRKLCVRGLTQQGK~~~ITNKQDIRTQFIKSASIETHFAWHKYKKDNVN~~AERELNSGTIPHEFKRQMYYTFGDFRDIFFGRDISSCGYIKKASENIKGILK~KENEKPED~~~~raj116var11CKRKE~~~~~KYKPWNCTSSQFKSGHTGACMPPRRQKLCVSSLTKQRK~~~IKEKEDIRTQFIKSAAIETHFAWLKYKEINTG~~ADNELQTGNIPDEFKRQMFYTFGDYRDIFFGTDISSCRYIKGTSKTIKSKLG~DQATTEKGGKHIraj116var08CGSKT~~~NEPYPGWDCTKIKVNNNTAAACIPPRRQKFCTTDLTQEGS~~LT~KEEHILTKFVNCAAKETHFAWHKYKKDNDK~~AESELNSGIIPEGFKRQMYYTFGDYRDIFFGTDISTYNYISGVSPKVITILQKENG~TKSRGIQK

1660 1670 1680 1690 1700 1710 1720 1730 1740 1750 1760 1770 1780 1790 1800. . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . |

1965-1 ~~~~~LEEWWNKHGKDIWEGMLCALSYDTKERSFKEDVRR~~~~~~~~~~~~~~~~~KLTTTYTYSTVKFS~EP~~~SGATLPTFAQTPQFLRWMTEWGEDFCNQRKGQLKKLEQGCSGYKCENSDE~~~~~~~~~~DKKQTCKTACEQY1965-3 FNNELLADWWNNHGKEIWEGMLCALTHDIEKEKN~~~~~~~~~~~~~~~~~~~~~~~QIKNTYSYENLKNP~TN~~~GTPSLEEFSSRPQFLRWFTEWGEEFCRTRKEKVEILKGKCAGCGVDAEGK~~~~~KCMG~~ECDECKKQCQAY1983-1_ FNNELLADWWNNHGKEIWEGMLCALSYDTKKQTMDKEVRQK~~~~~~~~~~~~~~LMNPQNKNTYANVKFS~DD~~~ET~TLEKFAQTPQFLRWFIEWGEHFCKEQKREFNTLKELCNKCNISDNGKG~~NKTCDDKKKCDECKRACKKY1983-3 ~~~~~~LNWWNEHKEAIWKGMLCGLSHASGNKETVRTQLT~~~~~~~~~~~~~~~~~SSQRHYEYDLVKFSGSD~~~NPLTLETFAQRPQFLRWFTEWGDEFCRERKKKEDDVKSKCTSDYEGCKNK~~~~~~~~~KTNGGNCVTACKEYIT4var20 SNNELLDDWWDQHGKDIWEGMLCALTHKISDE~~~~~~~~~~~~~~~~~~~~~EKKKEIKNKYSYKKLNESPK~~~~GSNKVEDFAKKPQFLRWFIEWGDEFCAQREEKEAKVKVSCSDAKDYDGCKN~~~~~~~~TKSNASCVSACKVYIT4var06 SKNELLDDWWDEHGHEIWEGMLCALTYKDKTSGSGGEGKTTITQDESLKEALWDEASNKPKNHDYNSVTISSVGPSGEKTYLSKFAERPQFLRWFIEWSDEFCRERKKKEEEVKSKCT~~SDYEGCKK~~~~~~~~TNGKTSCANACEAYMAL6P1.316 FNNVLLEDWWKEHGPEIWEGMLCALTNGLTDDEKK~~~~~~~~~~~~~~~~~~~~~NEIKTKYSYDELNNAKKG~~~~DSSLEEFAQTSQFLRWMTEWGEDFCKKRKEQVETLQKACKFYECN~INAED~~~~~~~~~TKTKCEKECKVYPF08_0140 YKNELLDDWWNEHGKEIWDGMVCALCYDTKTKEFKNEVRTQ~~~~~~~~~~~~~~LTGPNSKYQYGTVSFG~DK~~~QNTILSKFAERPPFLRWFIEWGEEFCKKRKEQVDQLVNVCKECNVSDSVISSGNKTCNDKEKCDACKKECTQYPFCLINvar30DGSKLLENWWNTNAKDIWDGMLCALEK~ITDKKNT~~~~~~~~~~~~~~~~~~~~~LTNNPSYKYDHVKFSGDN~~~~SPTLEKFAQTPQFLRWFIEWGEEFCKKRKEQVEKLRSECDGYECNEENMNQ~~~~~~~~~KKKTCEDACKAYIT4var19 DDNKKLQEWWTIHGPKIWEGMLCALTNGLSES~~~~~~~~~~~~~~~~~~~~~~EKKNILQDYSYNKLNNAEK~~~~DDCCLEKFASKPQFLRWYVEWSDEFCRERKKLEDKVEDVCIKAKDYEGCKN~~~~~~~~NKSNNSCVKVCKEYIT4var32b DDNKKLQEWWTIHGPKIWEGMLCALTNGLSES~~~~~~~~~~~~~~~~~~~~~~EKENILQDYSYNKLNNAEK~~~~DDCCLEKFASKPQFLRWYVEWSDEFCRERKKLEDKVEDVCIKAKDYEGCKN~~~~~~~~NKSNNSCVKVCKEYDD2var47 SNNELLDDWWDQHGKDIWEGMLCALTHKISDE~~~~~~~~~~~~~~~~~~~~~EKKKEIKNKYSYKKLNESPK~~~~GSNKVEDFAKKPQFLRWFIEWGDEFCAQREEKEAKVKVSCSDAKDYDGCKN~~~~~~~~TKSNASCVSACKVYighvar19 ~~~~~~~~WWKEHGHEIWEGMLCALEKASGATGTLTN~~~~~~~~~~~~~~~~~~~~~~NPNYTYPTIKFS~GD~~~KTTTLEEFAQTPQFLRWFIEWGEEFCREREKKENAVQKDCTQDYPGCKEK~~~~~~~~KTNGGNACNTACEAYraj116var11EHNEKRQEWWKKYGGHIWEGMLCGLTHGVTETDKKKNILDK~~~~~~~~~~~~~~YSYDKLKNEP~~~~~~~QN~~~GTTPLEDFAKKPQFLRWMIEWGDEFCKKRKEQVEKLR~~~SECDGYECNIS~GNNTKQD~~~~~~CEKACKVYraj116var08LDNVLLDDWWDEHGKEIWEGMLCALTHGVTNSEKK~~~~~~~~~~~~~~~~~~~~~TKIKTDYSYKELNKSQNG~~~~NASLEDFASKPQFLRWFTEWAEDFCNHQGEEIKKLENKCNFSTCEDEKDNQ~~~~~~~~~KKIQCHQACWNY

1810 1820 1830 1840 1850 1860 1870 1880 1890 1900 1910. . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . .

1965-1 QNWLSKWKEQYKKQSKKYFDDRKRQRFQSTTANDEVTASQYAYEYLKKALPKICPDD~~~~~~YCKCMD~~GESNNTILTKHNETHD~~~~~~~AHMPKSLDDEPEEVKGRCTC1965-3 KSWLQTWKGHYNKQKERYAKVKSTSPYNN~~~DNDVINSTEAYQYLNKKLTNIKCTSGTT~NVDCNCMK~~EPSKE~~PKKSPDGSN~~~~~~~DGMPASLDDEPEEVRGKCTC1983-1_ KNWLQTWKGHYNKQKERYANVKSTSPYSN~~~DEDIKQSQHAYQYLNKKLTNIKCTSGTT~NGDCNCMK~~EPSKE~~PKKSPDGST~~~~~~~DIMPASLDDEPEEVRGKCKC1983-3 KDYIGQKKVEYTKQKGKFDAEKITDKEGY~~~~~EGYAHKEAPEYLKKKCLDD~~~~~~~~~~TCNCMQKVKDTRNYWDKPNKTYTN~~~~~~~~~~~~~~~~SDLE~~KKCECIT4var20 EDYITKKKVEYTKQKGKFDAEKITDKEG~~~~~YEGFSTKDASEYLKKKCLDD~~~~~~~~~~TCNCMQKVKNNTEYWNTPNKTYTN~~~~~~~~~~~~~~~~SNLE~~KRCECIT4var06 KTYIKKKEIQYTTQKKKFDTEKNKEKKEKE~~~YENYTGKESHEYLKDKCFLH~~~~~~~~~~TCSCMEKVKTIGDYWKNPHETYDD~~~~~~~~~~~~~~~~NKLE~~TKCECMAL6P1.316 EQFIKQWKPQYEQQSKKFTTDKVQPEYAVD~~~ADVKKSTHAYQYLSKKLKKICQNGTTTDKCDYKCMENASRQPQTSACSQEQQQQ~NKSSTENNYPEAFDCPPKEIADKCNCPF08_0140 ENWLQTWKGHYNKQKERYAKVKSTSPYNND~~~NDVNKSTHAYEYLSKKLT~~~~~~~~~~NIPCTNGN~~TTGKCDYKCMDQRTST~~~~~~~DGMPASLEYPPKEINGKCDCPFCLINvar30QTWLKGWKDQYKKQSEKFTTDKVQSEYVVD~~~PDVKKSTHAYEYLSKKLKPICQSGTTTDKCDYTCMEKASRQPQTSACSQEQQQQQNKSSTQNHFPEAFDCPPKEIADKCNCIT4var19 ENYITGKKTQYESQEGKFNTEKRQKKPE~~~~~YNSYSKKDASEYLKDKCLDG~~~~~~~~~~TCDCMKKVKSIDNYWKNPHKTYDD~~~~~~~~~~~~~~~~NKLE~~TKCECIT4var32b ENYITGKKTQYESQEGKFNTEKRQKKPE~~~~~YNSYSKKDASEYLKDKCLDG~~~~~~~~~~TCDCMKKVKSIDNYWKNPHKTYDD~~~~~~~~~~~~~~~~NKLE~~TKCECDD2var47 EDYITKKKVEYTKQKGKFDAEKITDKEG~~~~~YEGFSTKDASEYLKKKCLDD~~~~~~~~~~TCNCMQKVKNNTEYWNTPNKTYTN~~~~~~~~~~~~~~~~SNLE~~KRCECighvar19 KNYITDKEKEYTKQKGKFKDDKTQNKSGY~~~~~NDTSTKDAPDYLKEKCLDG~~~~~~~~~~SCSCMEKVKNIDDYWKNPHKTYDD~~~~~~~~~~~~~~~~NKLE~~TKCECraj116var11QKWLQGWKENYKTQSEKYFQDKKDNKFESTSAKDEVTASQYAYEYLNKALQKLCD~~~~~~NVDCKCMD~~GQSNETPREPNKKSHD~~~~~~~AHMPKSLDEEPEEVKGRCTCraj116var08KAFLKKWKKQYKKQNIEYEGLRDS~~~~~~~~~INISQHMDAPKYLEENCKDKCS~~~~~~~~~~~CIKEADYT~~~~~~~~~~~~~~~~~~~~~HYNKSFEYPPEKFQEKCLC

NTS

DBLα

CIDRα

DBLβ

DBLγ

Domain cassette 8

10 20 30 40 50 60 70 80 90 100 110 120 130 140 150. . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . |

HB3var03 MG-SSASKFSKIVVGNETHKSARNVLEGFAKDIKGKASIDAEKHAYSLKGNLKDAKFNHD---FFKIKSDMPGNPCYLDFAFHSNTPGNQREYRHPCARSMNKNLFNLEG-AVCTNSKIKGNEEKINGAGACAPYRRRHICDLNLEHIDVPF11_0521 MGNAIP-ATPDPIFINESYKSARNVLENVARLIKAKATKDVKRHRNVLKGNLRNAKFYHP---FSKERPYYKS-ACDLDYRFHSNIWSGDNTYRHPCAG-RNRNRFSYEGEAECRISRITGNKTEH---GACAPYRRRHLCDYIFHQVNDIT4var07 -MGNAIPATPDPIFINESHKSARNVLEKFALEIKNKASDDAKKYRSSLKGDLKGAKFYHP----FSEHRPYYTGPCELEYEYHSNIWNGLKEYRHPCAGRNKTR-FSNESEEECNSSKITGNKTEH---GACAPYRRRQLCDYIFHQVNPDD2var32 MG-GNSSKGAPTYYTNESEKSARNVLENFAKDIKGKASNDAKKKGISLKGYLRQAKFYH----DFSKLYPNYRSPCDLNFWFHTNVWKRTPRERDPCYRRQPKNNPNLEG-AVCTNSKIKGNENKIIDIGACAPYRRRNICDYNLEHLNEigh_var27 MG-SSASKFSKIVVGNETHKSARNVLEGFAKDIKGKASSDAEKHAYSLKGNLKDAKFNHD---FFKINNYIPKNPCNLDFAFHSNTPGGEKQYRHPCARSMNKNLFNLEG-AVCTNSKIKGNEEKINGAGACAPYRRRHMCDLNLEHIDVigh_var23 MGTGSSTPSVPKDVKNESHKSARNVLDVLAEDIKKQAENKAQKHARSLKGKLSEAKFYQRLLKETNYVWYVPSNPCKLNYVFHTNVWHGNAEDRYPCLLRDKKRFSNEGE-AECNGGIITGNKG---GLGACAPYRRRHMCDYNLEFINEAAQ73928 MGHSYSHTTLTSDITNESHKSARNVLEKFALRIKNKATNDAKKHTRSLKGDLKRAKFNHD---FFKIKSDMPGNPCYLDFAFHSNTPGGEKQYRHPCARSMNKNLFNLEG-AVCTNSKIKGNEEKINGAGACASYRRRHICDLNLEHIDV1914-21 ------------------------------------------------------------------------------------------------------------------------------------------------------1983-15 ------------------------------------------------------------------------------------------------------------------------------------------------------

160 170 180 190 200 210 220 230 240 250 260 270 280 290 300. . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . |

HB3var03 HNVQNIHDLLGNVLVTAKYEGESIVEKHPNRGSSEVCTALARSFADIGDIIRGKDLYLGH-EQGNNKLEARLKTIFQNIKNK-------NKSPLDK-LSLEQVREYWWALNREDVWKALTCFADGSEEYFIQSSDKEHSFSSE-YCGHEQPF11_0521 NYIKTSDDLLGNLLVTAKYEGESIVNSYANSGMFNVCTALARSFADIGDIIRGKDLYLGN-GDYKEKVSNNLRAIFNKIYENLNDPKLKKHYQKDA-PNYYKLRDDWWNANREDVWKALTCNAPYEAQYFIKPSDKEHSFSSE-YCGHYKIT4var07 VHIKNSHDLLGNVLVTAKYEGESIVNSYANSGMFNVCTALARSFADIGDIIRGKDLYIGN-GDYKKKVSNNLKTIFKKIYDALKDTVKETYKDDP---NYYKLREHWWTVNRDQVWKAITCNAPYKAWYFIHSEDNTLLFSN-YKCGHYEDD2var32 RNVLNTHDLLGNVLVMAKREGESIVNSQANNGTLNVCTALARSFADIGDIVRGTDLFLGGPSQEKKKLEENLKKIFENIKN--------KNTKLST-LTLEKVREYWWALNRNDVWKALTCSAPYEAQYFIKSSDKEHSFSSE-YCGHYKigh_var27 HNVQNIHDLLGNVLVTAKYEGESIVEKHPNRGSSEVCTALARSFADIGDIIRGKDLYLGH-EQGNNKLEARLKTIFQNIKNK-------NKSPLDK-LSLEQVREYWWALNREDVWKALTCFADGSEEYFIQSENNTQLFSNP-KCGHEQigh_var23 QNVLTTHDLLGNVLVTAKYEGDSIVSNHPNKGSSEVCTLLARSFADIGDIIRGKDLFLGN-NKENEKLQENLKRIFKNIYANLKDPQALKHYKDDT-KNYYQLREDWWALNRNDVWKALTCSAPYDANYVRRKSDRTMDFTSQGYCGHSEAAQ73928 HNVQNIHDLLGNVLVTAKYEGESIVEKHPNRGSSEVCTALARSFADIGDIVRGKDLFLGN-NDNDKVKKEKLRGNLVNIFKKFK----EKYKDLKD-LQIDDIREYWWALNREDVWKALTCSAPYNAHYFIKSSDKEHSFSNE-HCGHYK1914-21 ------------------------------------XCESARSFADIGDIIRGKDLYLGH-EQGNNKLEARLKTIFQNIKNK-------NKSPLDK-LSLEQVREYWWALNREDVWKALTCFADGSEEYFIQSENNTQLFSNP-KCGHEQ1983-15 -------------------------------------------FADIGDIIRGKDMFKPN-P--QDKVQEGLKNVFKNIHENLPEPLKSHYSDKDKSGNYYKLREDWWTANRDKVWEAITCRAPQKANYFRKKSDGTLHFSSQGQCGHTE

310 320 330 340 350 360 370 380 390 400 410 420 430 440 450. . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . |

HB3var03 GNVP-TNLDYVPQFLRWFDEWADDFCRIKKIKLENVKNACRDEKKRKYCSLNGFDCTQTIWKKGVLHRSNECTGCLVKCNPYEIWLGNQREAFRKQKEKYENEIKTYVHDTGISNSNINNEYYKEFYKILKNNNYETANEFIKLLNEGRYPF11_0521 NGDPLTNLDYVPQFLRWFEEWAEEFCRIKKIKLENVKNACRDEKKRKYCSLNGFDCTQTIWKKKVFGRGNDCTNCSFKCFPYEIWLGNQREAFRKQKEKYAKEIEAYVTNRGIPKSSINNGYYKDFYNKLKEKTYNTVDEFINLLNEGRYIT4var07 DAPP-TNLDYVPQFLRWFDEWSEDFCRIKKIKLGRVKKACRDESSKLYCSHNGYDCTKTIRNKDICIRESKCTGCLVKCNPYEIWLENQRKEFDKQKEKYEKEINEKNTSRDSTNNSINNIYYEDFYKKYKEKTYNTVNEFIKLLNEGKYDD2var32 NGDPLTNLDYVPQFLRWFEEWAEEFCRIRYHKLKRIKDACRNDSKKLYCSHNGYDCTKTSWKKGVLHWSNECTDCSVKCKLYEIWLGNQREAFRKQKEKYEKEIETYVSNRGISNTNINNEYYKEFYEELK-KSYKSVHDFLTLLNEGRYigh_var27 GNVP-TNLDYVPQFLRWFDEWADDFCRIKKIKLELAKKACRDEKKRKYCSLNGFDCTQTIWKKGVLHWSNECTDCSVKCNLYEIWLGNQREAFRKQKEKYAKEIQTYTSKDAKTDSNINNKYYEEFYKILKNNNYETVNEFIKLLNEGRYigh_var23 TNVP-TNLDYVPQFLRWFDEWADDFCRKRNIKLKNVKDACRDEKKRKYCSLNGYDCTKTIWKKGVLHRSNECTGCLVKCNPYEIWLGNQREAFRKQKQKYEKEINEKNTSRDSTNNSINNIYYEDFYKKYKEKTYNTVDEFIKLLNEGRYAAQ73928 NGDPLTNLDYVPQFLRWFDEWADDFCRIRNHKLQKVKEACRDERRGKYCSLNGFDCTQTIWKKGVLHWSNECTDCSVKCNLYEIWLGNQREAFRKQKEKYAKEIEAYVTNRGIPKSSINNEYYGEFYKILKNNNYETANEFINLLNEGRY1914-21 GNVP-TNLDYVPQFLRWFDEWADDFCRIKKIKLENVKKACRDEKKRKYCSLNGYDCTKTIWKKGVLHRSNECTGCLVKCNPYEIWLENQRKEFEKQTKKYENEIKTYVHDSGISNSNINNEYYKEFYKKLKDNKYETANEFINLLNEGRY1983-15 GIVP-TYLDYVPQFLRWFDEWSEDFCRIKKXKLEKIKNACRDDSKKLYCSHNGYDCTKTIRNKDICIRESKCTGCLVKCNPYEIWLENQRKEFEKQTKKYAKEINGTNVSQDSTNNSINNKYYKEFYGKLYN-EYGNIDNFLKLLNEGKY

460 470 480 490 500 510 520 530 540 550 560 570 580 590 600. . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . |

HB3var03 CNKKEKIEEEEDIDFTNTNEKGTFYRSDYCQVCPDCGVECKNETCTPKTVIYPDCGKNEKYEPPGDAKNTEINVINSGDKEGYIFEKLSEFCTNENNENGKNYEQWKCYYDNKKNNNKCKMEINIANSKLKNKITSFDEFFDFWVRKLLIPF11_0521 CKKQ--KPEEENIDFIKTGEKGTFYRSDYCQVCPDCGVDCSSGTCIEK-KDDINCGKKINYEPPHGVKPIDIIVLYSGNEEGEITKRLSEFCTDSSNNKGKNYEQWKCYYKNGDDN-KCKMVKNSGNNITEEKIISFDEFFYVWVRKLLIIT4var07 CKEK--LPGKEIIDFTNIGEKGTFYRSEYCQPCPDCGVICENGKCVVKE-NGSNCRHYNIYEPAPDVKTTEINVIVSGDEQGIITKKLQDFCMNPNNENGTNNQIWKCYYKDEKEN-KCKVETKSGNSTYKEKITSFDEFFDFWVRKLLIDD2var32 CKTE--NVEEEVIDFN-SDMNTTFYRSDYCQVCPDCGVECNKGTCKKKP-NDSNCRNNEAYIPPRDVTPTKINVLYSGDGHGDITKKLKGFCSNPTDYDGKNYENWQCYYKSSEDN-KCQMTSLSQTLQKHHYVMSFYAFFDFWVRKFLIigh_var27 CKTK-KTKEEEDIDFTNADEKGTFYRSKYCQVCPDCGVICDGTTCNPKTEKYPDCGKNEKYEPPGDAKNTEINVIVSGNEQGDITEKLSEFCNDENKEN---YKKWKCYYESTYNN-ACKMENNGGINTSKEKITSFDEFFDLWVRNFLIigh_var23 CKTE--NVEEEAIDFN-SDMNTTFYRSKYCQVCPDCGVKCNGETCINKE-YDDECRNKQKYEPPIGLTPTKITVFYSGD-ESDITQKLQKFCKDKNNKNGKNYQKWQCYYKDSIDN-KCKMVKNSGNNITEDKITSFDEFFDLWVRNFLIAAQ73928 CKKQ--KPEEENIDFTKTGEKDTFYRSDYCQVCPDCGVICDGTTCKPKEKKYPECLNKEIYTP-NGAETTEINVINSGDKEGYIFEKLEDFCINGIKSNGKNYEQWKCYYKHIDDN-KCKMVKNSGNNITEEKIISFDEFFYVWVRKLLI1914-21 CNKKEKIEEEV-INFTKTGEKDTFYRSDYCQVCPDCGVECKNETCKPKEKKYPECLNKEIYTPNG-AKTTEINVIDSGDKQVGITEKLSEFCINENNENGKNYQKWECYYKHIDDN-KCKMVKNSGNNITEEKIISFDEFFDFWVRKLLI1983-15 CKKN--LEKEEDITFTNIGEKRTFYRSKYCQVCPDCGVNCDDKTCKEKP-NDGDCGNKVKYEFPKGKPTTEITVLYSGDEQGIITKKLQDFCRKPTELNENNYQKWKCYYKDEKEN-KCKVETKSGNSTYKEKITSFDEFFDFWVRNFLI

610 620 630 640 650 660 670 680 690 700 710 720 730. . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | . . . . | .

HB3var03 DTIKWETELTYCINNTDVTDCN-KCNKNCVCFDKWVKQKEDEWTNIMKLFTNKHDIPKKYYLNINDLFDSFFFQVIYKFNEGEAKWNELKENLKKQIASSKANNGTKDSEAAIKVLFNHIKEIATICKDNNTNEGCPF11_0521 DSIKWENELNNCIDNTS-THCNKECNKNCECFDKWVKKKEDEWKNVKNVFENKNGTSHNYYNKLNGLFKGFFFEVMDKLNKDETKWNKLIESLRTKIDSSKENIGTGNTQDTIKVLLDHLKETATICKDNNTNEACIT4var07 DTIKWETELTYCINNTTNADCNNECNKNCVCFDKWVKQKEKEWKNIMDLFTNKHDIPKKYYLNINDLFNSFFFQVIYKFNEGEAKWNKLKENLKKKTESSKKNKGTKDSEAAIKVLFDHLKETATICKDNNTNEACDD2var32 DTIKWENELKNCINNTT-TDCSDGCNKHCVCFDKWVKQKEKEWNSIKKLFTKKNNVPQPYYTNINNLFDSFYFQVMYELNHDEAKWKELTQELRNKINSSKKNTDAADSKDAIELLLEYLKEKSTICKDNNTNEACigh_var27 DTIKWENEVKTCINNTTNADCNNECNKNCICFDKWVKQKEEEWKNIMDVFKKEENIPKKYYLNINDLFDSFFFEVLSEIKQGEAKWNELKENLKKQIASSKANNGTKDSEGAIKVLFDHLKETATICKDNNTNEACigh_var23 DTIKWENEVKTCINNTTNADCNNECNKNCICFDKWVKQKEQEWKNVKKVFENQKDIPKKYYTNINDLFNSFFFQVIYKFNEGEAKWKELKENLKKQIASSKQKTGSKDLQDAIELLLEYLKEKSTICKDNNTNEACAAQ73928 DSIKWENELNNCIDNTS-THCNKECNKNCVCFDKWVKKKEDEWKNVKNVFENKNGTSHNYYNKLNGLFKGFFFEVMNEFKQDETKWNKLKENIKQIIDSSKENIGTGNTQDTIKVLLDHLKETATICKDNNANEAC1914-21 DTIKWETELTYCINNTNVTDCN-KCNKNCVCFDKWVKQKEQEWKNIMDLFTNKHDIPKKYYLNINDLFNSFFFQVIYKFNEGEAKWNKLKENLKKKXESSKQNRGTEDSEAAIKVLFDHLKENATICKDNNTNEAC1983-15 DTIKWENEVKTCINNTTNADCNNECNKNCVCFDKWVKQKEKEWKNVKKVFENQKDIPKKYYTNINDLFNSFFFQVIYKFNEGEAKWKELTQELKKKXDSSKEKSGTKDSESAIELLLEYLKEKSTICKDNNTIEAC

NTS

DBLα

CIDRα

Domain cassette 13

Figure S4. Amino acid alignments of PfEMP1 domain cassette 8 and 13 sequences. Amino acids are shaded if shared identity is at least 70%.

GENE Domain Obs UPS Mean CI low CI high

PFD1235w DBLβ3 543 UPSA 0,013 0,005 0,026

PFE1640w DBLβ1 543 UPSA 0,018 0,009 0,034

MAL6P1.314 DBLα1.3-DBLε8 543 UPSA 0,031 0,018 0,05

HB3var04 DBLγ3 543 UPSA 0,033 0,02 0,052

PF08_0141 DBLγ14 543 UPSA 0,035 0,021 0,054

IT4var22 DBLγ11 543 UPSA 0,035 0,021 0,054

PFE1640w DBLγ15 543 UPSA 0,037 0,023 0,056

PFE1640w DBLε1 543 UPSA 0,037 0,023 0,056

PF08_0141 DBLζ5 543 UPSA 0,037 0,023 0,056

HB3var01 DBLγ13 543 UPSA 0,041 0,026 0,061

IT4var02 DBLδ1 543 UPSA 0,044 0,029 0,065

PF11_0008 DBLβ9 543 UPSA 0,046 0,03 0,067

PF11_0008 DBLδ5 543 UPSA 0,05 0,033 0,072

PFD0020c DBLβ12 543 UPSA 0,05 0,033 0,072

PFE1640w DBLζ1 543 UPSA 0,052 0,035 0,074

HB3var05 CIDRδ1 543 UPSA 0,052 0,035 0,074

PFD1235w DBLβ3 543 UPSA 0,052 0,035 0,074

IT4var02 CIDRβ3 543 UPSA 0,053 0,036 0,076

IT4var02 DBLβ9 543 UPSA 0,055 0,038 0,078

PF13_0003 DBLβ9 543 UPSA 0,057 0,039 0,08

IT4var08 DBLζ6 543 UPSA 0,057 0,039 0,08

HB3var06 DBLγ6 543 UPSA 0,059 0,041 0,082

PFA0015c DBLα1.3-DBLε8 543 UPSA 0,064 0,045 0,089

PF08_0141 DBLβ6 543 UPSA 0,064 0,045 0,089

PF11_0008 DBLδ5 543 UPSA 0,068 0,048 0,093

IT4var02 DBLγ12 543 UPSA 0,07 0,05 0,095

IT4var60 DBLε12 543 UPSA 0,07 0,05 0,095

PF08_0141 DBLε4 543 UPSA 0,072 0,052 0,097

PF13_0003 CIDRδ1 543 UPSA 0,074 0,053 0,099

PFD1235w DBLγ13 543 UPSA 0,081 0,059 0,107

HB3var03 DBLδ5 543 UPSA 0,085 0,063 0,111

HB3var02 CIDRα1.6 543 UPSA 0,087 0,064 0,113

PF11_0008 DBLg12 543 UPSA 0,087 0,064 0,113

PFD0020c DBLγ11 543 UPSA 0,094 0,071 0,122

IT4var08 CIDRγ3 543 UPSA 0,096 0,072 0,124

PF11_0008 CIDRβ4 543 UPSA 0,099 0,076 0,128

IT4var64 DBLβ7 543 UPSA 0,103 0,079 0,132

HB3var05 DBLβ9 543 UPSA 0,105 0,08 0,134

PFD1235w CIDRα1.6 543 UPSA 0,11 0,085 0,14

PF11_0521 CIDRα1.4 543 UPSA 0,112 0,087 0,142

HB3var01 CIDRδ2 543 UPSA 0,123 0,097 0,154

IT4var02 CIDRδ1 543 UPSA 0,127 0,1 0,158

IT4var18 CIDRα1.6 543 UPSA 0,127 0,1 0,158

IT4var18 DBLγ2 543 UPSA 0,151 0,122 0,184

HB3var35 CIDRδ1 543 UPSA 0,162 0,132 0,196

IT4var64 DBLα1.5 543 UPSA 0,169 0,139 0,204

HB3var03 CIDRα1.4 543 UPSA 0,201 0,168 0,237

HB3var05 DBLα1.6 543 UPSA 0,212 0,178 0,249

HB3var06 DBLα1.5 543 UPSA 0,217 0,183 0,254

PFD0020c DBLα1.2 543 UPSA 0,217 0,183 0,254

PF08_0140 DBLβ12 543 DC8 0,055 0,038 0,078

IT4var20 DBLα2 543 DC8 0,066 0,047 0,091

PFD0020c DBLγ6 543 DC8 0,09 0,068 0,118

MAL6P1.316 DBLγ4 543 DC8 0,11 0,085 0,14

PF08_0140 DBLβ12 543 DC8 0,12 0,094 0,15

IT4var06 DBLα2 543 DC8 0,12 0,094 0,15

PFD0020c CIDRα1.1 543 DC8 0,123 0,097 0,154

IT4var20 CIDRα1.1 543 DC8 0,158 0,129 0,192

MAL6P1.1 CIDRα4 543 UPSB 0,006 0,001 0,016

MAL6P1.4 DBLγ13 543 UPSB 0,006 0,001 0,016

PFL0020w DBLζ5 543 UPSB 0,007 0,002 0,019

IT4var46 DBLζ3 543 UPSB 0,009 0,003 0,021

PFL0005w CIDRα2.2 543 UPSB 0,015 0,006 0,029

HB3var09 DBLε6 543 UPSB 0,017 0,008 0,031

PFD0005w DBLγ5 543 UPSB 0,017 0,008 0,031

PFL1955w CIDRα3.4 543 UPSB 0,018 0,009 0,034

PFD0005w CIDRα2.10 543 UPSB 0,018 0,009 0,034

Dd2var01 CIDRα3.1 543 UPSB 0,022 0,011 0,038

HB3var21 DBLε4 543 UPSB 0,022 0,011 0,038

PF08_0103 CIDRα2.2 543 UPSB 0,024 0,013 0,041

PFL0020w DBLβ5 543 UPSB 0,026 0,014 0,043

PFL0020w DBLε4 543 UPSB 0,031 0,018 0,05

HB3var22 DBLε2 543 UPSB 0,031 0,018 0,05

PFL2665c CIDRα2.3 543 UPSB 0,031 0,018 0,05

PF08_0140 CIDRα1.6 543 UPSB 0,035 0,021 0,054

MAL6P1.316 DBLε12 543 UPSB 0,035 0,021 0,054

IT4var15 CIDRα3.5 543 UPSB 0,037 0,023 0,056

PFC0005w CIDRα2.4 543 UPSB 0,039 0,024 0,059

PFD0005w DBLδ1 543 UPSB 0,039 0,024 0,059

MAL6P1.4 DBLδ4 543 UPSB 0,039 0,024 0,059

MAL6P1.4 DBLε7 543 UPSB 0,041 0,026 0,061

HB3var13 CIDRα3.2 543 UPSB 0,046 0,03 0,067

MAL6P1.4 DBLε3 543 UPSB 0,052 0,035 0,074

PFA0765c DBLδ1 543 UPSB 0,052 0,035 0,074

MAL6P1.4 DBLβ5 543 UPSB 0,055 0,038 0,078

HB3var27 CIDRα3.1 543 UPSB 0,055 0,038 0,078

MAL6P1.316 DBLζ3 543 UPSB 0,063 0,044 0,086

MAL6P1.4 DBLε2 543 UPSB 0,064 0,045 0,089

HB3var40 DBLζ6 543 UPSB 0,074 0,053 0,099

PFD0625c DBLδ1 543 UPSB 0,081 0,059 0,107

IT4var13 CIDRγ9 543 UPSB 0,109 0,084 0,138

IT4var21 CIDRα3.1 543 UPSB 0,138 0,11 0,17

HB3var48 DBLζ4 543 UPSB 0,147 0,119 0,18

HB3var22 DBLε3 543 UPSB 0,265 0,229 0,304

PFD0615c DBLδ1 543 UPSC 0,002 0 0,01

PF07_0049 DBLδ1 543 UPSC 0,024 0,013 0,041

MAL6P1.4 CIDRγ1 543 UPSC 0,004 0 0,013

HB3var25 CIDRγ1 543 UPSC 0,101 0,077 0,13

IT4var01 DBLδ2 543 UPSC 0,029 0,017 0,047

Figure S5. Seropositivity rate (number of children with antibodies/total number of children x 100) of 99 different PfEMP1 domains based on measurements of anti-PfEMP1 IgG in plasma from 543 young children from North-Eastern Tanzania. Cut-off was based on the reactivity of Danish donors never exposed to malaria. Recombinant PfEMP1 domains are annotated with gene of origin, recombinant domain type and the UPS group assigned for testing differences in seropositivity between UPSA, DC8, other UPSB or UPSC type PfEMP1. Lower and upper values of seropositivityrate (CI low & CI high) for confidence intervals are given.

Table S1: Additional clinical characteristics of the malaria patients

1: Patients requiring admission to District Hospital classified as complicated because the fulfilled the criteria for severe anemia, cerebral malaria, respiratory

distress and/or hyperparasitaemia

2: Patients admitted to hospital with Blantyre coma score=5, Haemoglobin>8g/dl, not prostrated, no symptoms of respiratory distress, parasite density < 200.000

/µl, plasma lactate<7mmol/l

3: Patients admitted with a haemoglobin<5g/dl and Blantyre coma score = 5

4: Patients unable to sit (if able to sit prior to the admission) or unable to suck

5: Patients diagnosed and treated during village surveys

Complicated

hospital

infections1

N=88

Hospitalised

but no signs

of severity2

N=12

Severe

anaemia

without

cerebral

symptoms3

N=37

Cerebral

malaria with

haemoglobin

>8g/dl

N=9

Lactate

>7mmol/l

N=14

Prostrated4

N=53

Village

infections5

N=22

Age mean ±sd (years)

Min/max

2.3±1.2

0.51–4.8

2.1±1.2

0.63–4.5

2.1±0.9

0.58–4.0

2.3±1.4

0.61–4.0

1.7±1.3

0.63–4.4

2.5±1.2

0.51–4.9

9.4±5.0

1.4–18.3

Females (%) 42.0 41.7 45.9 22.2 50.0 35.8 56.3

Parasite density median (p/ul)

Percentile 25 & 75

31,780

5,110-170,345

47,410

29,619-78.510

14,280

1,505-64,750

45,955

4,795-101,030

20,615

4,480-200,480

32,410

4,480-135,415

7,200

5,000-16,000

Temperature median (⁰C )

Percentile 25 & 75

38.3

37.5-39.1

38.6

38.03-39.5

38.1

37.1-38.8

37.5

36.8-38.6

387.9

37.5-39.0

38.3

37.7-39.1

36.7

36.6-37.2

Haemoglobin mean ±sd (g/dl)

Min/max

5.5±2.2

1.7-11.9

9.4±1.1

8.1-11.8

3.9±0.7

1.7-4.9

9.3±0.9

8.2-10.5

4.9±1.8

1.7-7.9

5.8±1.9

1.7-10.5

11.9±1.6

8.5-15.3

Coma score

Percentile 25 & 75

Min/max

3.6

2-5

0-5

5

5-5

5-5

5

5-5

5-5

1.3

0-2

0-2

3.3

3-5

0-5

2.9

2-5

0-5

5

5-5

5-5

P-lactate Mean ± sd (mM) 4.7±2.9 3,6±1.1 4.1±2.2 5.5±4.5 9.8±3.1 5.1±3.3 n.d.

Mortality (%) 10.2 0 0 11.1 14.2 13.2 0

Table S2: Var subtype transcript levels in P. falciparum infected children; discrete symptoms. Var subtype transcript levels (median Tu level and 95 confidence interval) in hospitalized patients with uncomplicated malaria vs. patients with no signs of severity, severe anemia and no cerebral symptoms,

cerebral malaria and no symptoms of anemia, lactate >7mmol/l, prostration or individuals diagnosed with P. falciparum in their village. All p-values are calculated by comparison of severe syndrome group

to uncomplicated malaria patient group using Wilcoxon rank-sum tests.

Primer domain subclass

Domain cassette Group

Uncomplicated

n=40

Complicated hospital

infections

N=88 p

Hospitalised no signs of

severity

N=12 p

Severe anaemia

no cerebral

symptoms N=37 p

Cerebral malaria

w/ haemoglobin

>8g/dl N=9 p

Lactate

>7mmol/l

N=14 p

Prostrated

N=53 p

Village

infections

N=22 p

DBLa1.1 DC1 A 1 [1;1.5] 1.1 [1;2.4] 0.0975 1 [1;1] 0.1456 1 [1;3.3] 0.2698 1.1 [1;11.2] 0.2394 2.2 [1;3.4] 0.3278 1.7 [1;2.9] 0.1047 1 [1;1] 0.016

var2csa DC2 E 4.7 [1.8;8.2] 2.7 [1.4;4.7] 0.3422 5.5 [1;12.1] 0.9244 1.7 [1;3.2] 0.1078 3.2 [1;17.9] 0.5119 2.9 [1;14.4] 0.391 3.3 [2.3;5.8] 0.4513 1 [1;4.6] 0.0934

DBLe8 DC3 A 1 [1;1] 1 [1;1] 0.1106 1 [1;1] 1 1 [1;1.1] 0.0669 1 [1;1.6] 0.6302 1 [1;1] 0.5337 1 [1;1] 0.1806 1 [1;1] 0.7815

DBLb7&9 DC5 A 1 [1;1] 1 [1;1] 0.9254 1 [1;1] 0.1125 1 [1;1] 0.6884 1 [1;5.2] 0.9001 1 [1;1.3] 0.6434 1 [1;1] 0.7907 1 [1;1] 0.0609

DBLg DC5 A 1 [1;1] 1 [1;1] 0.0834 1 [1;1] 0.8936 1 [1;1] 0.38 1 [1;16] 0.2263 1 [1;1.5] 0.3778 1 [1;1] 0.0445 1 [1;1] 0.0867

DBLz5 DC6 B(A/C) 1 [1;1.6] 1 [1;1.3] 0.5573 1 [1;1.1] 0.1788 1 [1;1] 0.4142 1 [1;5.1] 0.6848 1 [1;7.1] 0.5189 1 [1;4.6] 0.2914 1 [1;1] 0.0105

DBLg DC6 B(A/C) 1 [1;1.2] 1 [1;1.4] 0.3087 1 [1;1] 0.0212 1 [1;2.1] 0.6563 1.1 [1;8.7] 0.3173 1.1 [1;5.8] 0.1632 1.4 [1;2.8] 0.0705 1 [1;1] 0.0337

DBLe2 DC7 B( C) 1 [1;1] 1 [1;1] 0.5673 1 [1;1] 0.4432 1 [1;1] 0.6811 1 [1;1] 0.5093 1 [1;1] 0.276 1 [1;1] 0.1727 1 [1;1] 0.1924

DBLe3 DC7 B(A/C) 1 [1;1] 1 [1;1] 0.9664 1 [1;4.7] 0.5998 1 [1;1] 0.3974 1 [1;2.3] 0.5841 1 [1;1] 0.276 1 [1;1] 0.8954 1 [1;1] 0.2163

DBLa_CIDRa DC8 B 1 [1;1.1] 3.5 [1.3;6.7] 0 1 [1;1.2] 0.4356 3.5 [1;7.6] 0.0004 1 [1;18.1] 0.6927 5.3 [1;9.9] 0.004 3.8 [1.4;8.8] 0 1 [1;1] 0.0076

DBLg4/6 DC8 B 1 [1;1.7] 3.1 [1.8;7.6] 0.0004 1 [1;2.5] 0.5485 5 [1.2;12.2] 0.0029 3.1 [1;37.6] 0.0416 2.1 [1;33] 0.1032 2.3 [1;5.9] 0.0033 1 [1;1] 0.0062

CIDRa1.1 DC8 B 1.1 [1;3.2] 17.8 [9;24.4] 0 1 [1;4.6] 0.1851 15 [5;31.2] 0.0017 7.8 [1.4;28.5] 0.0481 16.2 [4.5;34.5] 0.0026 19.6 [7.8;31.9] 0 1 [1;1] 0.0093

DBLb12 & DBLb3/5 DC8 B/A 7.3 [1.1;13.2] 26.4 [15.3;33.3] 0.0007 2.7 [1;14.1] 0.8445 27.1 [13.1;33.7] 0.0022 13.5 [1;38.5] 0.4288 18.3 [6.9;38.3] 0.0876 27.6 [13.6;36.4] 0.0011 1 [1;1] 0.0007

DBLz4 DC9 B 1.6 [1;4.1] 1 [1;1.2] 0.0307 1.2 [1;5.3] 0.6095 1 [1;1.2] 0.0174 1 [1;6.7] 0.7047 1 [1;2.2] 0.0416 1 [1;1] 0.0273 1 [1;1] 0.0063

DBLg of DC9 DC9 B 1 [1;1.2] 1 [1;1] 0.0139 1 [1;1.2] 0.2787 1 [1;1] 0.018 1 [1;2.2] 0.8619 1 [1;1.3] 0.1247 1 [1;1] 0.0583 1 [1;1] 0.0279

DBLz6 DC10 B 1 [1;1] 1 [1;1] 0.9784 1 [1;2] 0.5346 1 [1;1] 0.8822 1 [1;1] 0.458 1 [1;1.2] 0.8639 1 [1;1] 0.2136 1 [1;1] 0.1504

DBLe12 DC12 B/A 1 [1;1] 1 [1;1] 0.2642 1 [1;1.1] 0.8039 1 [1;1] 0.5924 1 [1;1] 0.257 1 [1;1] 0.1161 1 [1;1] 0.2944 1 [1;1] 0.0163

DBLa1.7 DC13 A 1.2 [1;3] 1 [1;5.8] 0.4874 1 [1;6.6] 0.492 1 [1;6.3] 0.5981 16.5 [1;50.3] 0.1815 1 [1;10.2] 0.8618 1 [1;9.5] 0.3353 1 [1;1] 0.0221

CIDRa1.4 DC13 A 1 [1;1] 1 [1;1] 0.0013 1 [1;1] 0.5127 1 [1;1] 0.0012 4.3 [1;33.7] 0 1 [1;1] 0.3446 1 [1;1] 0.0001 1 [1;1] 0.5165

DBLa1.5/6a DC16 A 1.1 [1;2.4] 1 [1;2.1] 0.9603 1 [1;4.8] 0.5384 1 [1;2.4] 0.6208 1 [1;57.9] 0.9779 1 [1;20.2] 0.2616 1.1 [1;3.1] 0.6657 1 [1;1] 0.0138

DBLa1.5/6b DC16 A 3 [1;5.7] 1.5 [1;4] 0.5996 1 [1;15.1] 0.2251 1.3 [1;5.2] 0.2979 3.7 [1;109.8] 0.643 1 [1;2.9] 0.0385 3.4 [1;4.9] 0.9933 1 [1;1] 0.003

CIDRd DC16 A 1 [1;1.7] 1.1 [1;2.7] 0.3384 1 [1;2.6] 0.1503 1 [1;1.9] 0.8508 1.1 [1;39.9] 0.5017 3.1 [1;33.8] 0.2233 1 [1;3.2] 0.341 1 [1;1] 0.1491

DBLa0.16 DC19 B 1 [1;1.5] 1.7 [1;2.7] 0.053 1 [1;2.1] 0.892 1.4 [1;2.6] 0.3518 1.8 [1;20.5] 0.0659 1.3 [1;3] 0.6775 2.5 [1.5;4] 0.0086 1 [1;1] 0.1179

CIDRa3.4 DC19 B/C 3.2 [1.6;4.8] 2.3 [1.7;4.5] 0.9771 4.4 [1;13.5] 0.611 1.8 [1.1;3] 0.2816 7.6 [2;22.6] 0.0814 1.7 [1;5.2] 0.1725 4.4 [2;9.4] 0.3362 1 [1;2] 0.0139

DBLa0.9 DC20 B 1.1 [1;2.2] 1 [1;1.7] 0.5756 1 [1;6.5] 0.6248 1 [1;2] 0.5475 1 [1;3.2] 0.6374 1.2 [1;3.2] 0.8538 1 [1;1.6] 0.4405 1 [1;1] 0.0135

DBLa2/a1.1/2/4/7 A 18.9 [5.5;26.3] 56.1 [36.6;77.1] 0.0001 5.2 [1.2;43.4] 0.3754 58.4 [31.5;83.6] 0.0009 53.7 [1.7;164] 0.2662 52.7 [16.3;99.7] 0.0379 48.5 [32.3;63.4] 0.0005 2.3 [1;9.3] 0.011

DBLa1.4 A 1 [1;1] 1 [1;1] 0.2298 1 [1;6.6] 0.4858 1 [1;1] 0.6305 1 [1;5.7] 0.8834 1 [1;1.2] 0.462 1 [1;1.1] 0.085 1 [1;1] 0.5552

CIDRa1.6 A 1 [1;1] 1 [1;1] 0.1941 1 [1;1] 0.8463 1 [1;1] 0.6588 1 [1;1] 0.7035 1 [1;1] 0.3726 1 [1;1] 0.0891 1 [1;1] 0.28

CIDRa1.7 A 1 [1;1.6] 1 [1;1] 0.7361 2.1 [1;19.9] 0.1971 1 [1;3.4] 0.5698 1 [1;12.6] 0.5739 1 [1;6.9] 0.5735 1 [1;1.1] 0.7723 1 [1;1] 0.0609

CIDRa1.4 & CIDRa1 A 1 [1;1.3] 1.3 [1;3.6] 0.1359 1.3 [1;3.4] 0.3939 1 [1;3.7] 0.3134 6.7 [1;35.1] 0.0266 1.6 [1;6.6] 0.2928 1.4 [1;4.4] 0.1315 1 [1;1] 0.018

DBLa0.6/9 B 1 [1;3.5] 1.7 [1;2.4] 0.9028 2.2 [1;22.6] 0.2133 1.3 [1;3.6] 0.9564 2 [1;2.4] 0.6044 1.4 [1;3.9] 0.9449 1.9 [1;2.6] 0.7717 1 [1;1] 0.0094

DBLb3 A 1 [1;3.1] 3.5 [1.8;12.1] 0.0109 1 [1;5.1] 0.8185 2.6 [1;9.7] 0.0896 7.9 [1;21.9] 0.2823 15.1 [2.4;30.2] 0.0129 7.9 [1.8;16.2] 0.0057 1 [1;1] 0.0593

DBLa1 not var3 A 13.3 [5.1;21.9] 48.9 [36;68.1] 0 6.3 [1;30.4] 0.3411 37.1 [31.4;53.1] 0 86.1 [16;109.8] 0.0183 52.9 [24.1;89.3] 0.0028 55.2 [37.5;78] 0 1.6 [1;12.8] 0.0665

DBLg9 A/B/C 1 [1;1] 1 [1;1] 0.8608 1 [1;1] 0.4057 1 [1;1] 0.8016 1 [1;1.4] 0.659 1 [1;1] 0.5337 1 [1;1] 0.6174 1 [1;1] 0.9019

CIDRg1/2 B 1 [1;1] 1 [1;1] 0.337 1 [1;4.1] 0.3327 1 [1;1] 0.8496 1 [1;1] 0.3593 1 [1;1] 0.0752 1 [1;1] 0.2649 1 [1;1] 0.185

DBLa0.1 B 1 [1;1] 1 [1;1] 0.876 1 [1;1.3] 0.6574 1 [1;1] 0.5646 1 [1;2.4] 0.1078 1 [1;1] 0.0969 1 [1;1] 0.6434 1 [1;1] 0.0565

CIDRa2.2 B 1 [1;1] 1 [1;1] 0.344 1 [1;1] 0.2449 1 [1;1.6] 0.1862 1 [1;2.3] 10000 1 [1;1] 0.6069 1 [1;1.1] 0.5058 1 [1;1] 0.2594

CIDRa2.3/5/6/7/9/10 B 3.6 [2.4;5.3] 4.3 [2.6;8.5] 0.684 2.9 [1.1;6.3] 0.3901 4.1 [2;9.7] 0.6631 6.2 [1.1;11.2] 0.7755 5.4 [1;12] 0.9303 4.6 [1.9;8.6] 0.8599 1 [1;1.1] 0

CIDRg2/9 B 1 [1;1] 1 [1;1] 0.7415 1 [1;1] 0.0718 1 [1;1] 0.7016 1 [1;1.7] 0.69 1 [1;1.9] 0.4156 1 [1;1] 0.4761 1 [1;1] 0.0214

DBLb5 B(A/C) 1 [1;1] 1 [1;1.1] 0.0441 1 [1;1] 0.7415 1 [1;1.1] 0.1621 1 [1;1.3] 0.7067 1 [1;3.8] 0.5045 1 [1;1.5] 0.0178 1 [1;1] 0.0115

CIDRg B/C 9.7 [3.9;19.7] 9.1 [6.8;13.1] 0.674 5.2 [1;21.3] 0.1312 9.9 [6.6;23.6] 0.5527 17 [9.5;37.4] 0.3319 5.1 [1.8;17.4] 0.4271 8.4 [5.5;12.9] 0.9838 2.3 [1;4] 0.0035

CIDRa3.1-3 B/C 1 [1;1] 1.1 [1;1.6] 0.0178 1 [1;1] 0.1566 1 [1;1.4] 0.0779 1.2 [1;2.6] 0.0676 1 [1;2.1] 0.342 1 [1;1.8] 0.0374 1 [1;2.3] 0.798

target (primer name) dbla1.5/6a cidra1.7 cidra3.4 …...target (primer name) dbla1.5/6a cidra1.7...

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