percobaan 20 aktivitas enzim intraseluler uji imvic dan tsi

7/24/2019 Percobaan 20 Aktivitas Enzim Intraseluler Uji IMViC Dan TSI

1/25

MIKROBIOLOGI AIR

IL-2203

MODUL AKTIVITAS BIOKIMIA MIKROORGANISME

Percobaan 18 A!"#$#"a% En' E!%"ra%e()(er M#!roor*an#%'e

Percobaan 1+ A!"#$#"a% En' In"ra%e()(er U,# er'en"a%# .an O!%#.a%#

Percobaan 20 A!"#$#"a% En' In"ra%e()(er U,# IMV#/ .an TSI

Percobaan 21 A!"#$#"a% B#o!#'#a (a#n

Na'aNIM Pan,# Ara ).a14516016 La%"r# Na"a%#a S7 14516014

Kan#a Sa('aa1451601

Ke(o'9o! 4

Tan**a( Pra!"#!)' 21 O!"ober 2014

Tan**a( Pen*)'9)(an La9oran 6 No$e'ber 2014

P: Mo.)( #"r#ana;a"#

A%#%"en A'a. M)(a%#r

La)ren"#a M)"#ara

S#"# N)r


2/25

AKTIVITAS ENZIM INTRASELULER : UJI IMVIC DAN TSI

I7 TU:UAN

17 Me'9e(a,ar# 9ro%e.)r )n")! 'e'be.a!an an"ar an**o"a =a'#(#

En"erobac"er#aceae

27 Me'be.a!an an"ara ba!"er# En"erobac"er#aceae .en*an *r)9 a"a)

!e(o'9o! ba!"er# )%)% a"a) ba!"er# #n"e%"#na( ba"an* (a#nna

II7 PRINSIP

Perbe.aan )"a'a an"ara !e(o'9o!-!e(o'9o! an* "er'a%)! =a'#(#

En"erobac"er#aceae .a9a" .#(a!)!an .en*an 'e'9e(a,ar# !ara!"er#%"#!

.a%ar b#o!#'#a .an rea!%# en'a"#!na 9a.a %)b%"ra"-%)b%"ra" %9e%#=#!7

Sa(a %a")na a.a(a ),# IMV#/ "er.#r# .ar# %er# ),# #n.o(> 'e"#( 'era>

Vo*e% Pro%!a)er .an 9en**)naan %#"ra"7 U,# A*ar Tr#9(e S)*ar Iron ?TSI@

'er)9a!an ),# an* .#.e%a#n )n")! 'e'be.a!an !e(o'9o! a"a) *en)%

an* 'er)9a!an =a'#(# En"erobac"er#aceae> an* 'er)9a!an ba!"er# *ra'

ne*a"#= berben")! ba"an* an* 'a'9) 'e'=er'en"a%# *()!o%a .an

'en*a%#(!an a%a'> %e(a#n ,)*a )n")! 'e'be.aan ba!"er# =a'#(#

En"erobac"er#aceae .en*an ba!"er# #n"e%"#na( *ra' ne*a"#= berben")!

ba"an* (a#nna7 Perbe.aan #n# .#.a%ar!an 9a.a 9o(a =er'en"a%# !arbo#.ra"

.an 9ro.)!%# #.ro*en %)(=#.a o(e ber'aca' !e(o'9o! or*an#%'e

#n"e%"#na(7

III7 TEORI DASAR

/#r# =#%#o(o*# a"a)9)n b#o!#'#a 'er)9a!an !r#"er#a an* a'a" 9en"#n* .#

.a(a' #.en"#=#!a%# %9e%#'en ba!"er# an* "a! .#!ena( !arena %ecara

'or=o(o*#% b#a!an a"a) 9)n %e( ba!"er# an* berbe.a .a9a" "a'9a! %er)9a>

"an9a a%#( 9en*a'a"an =#%#o(o*#% an* 'e'a.a# 'en*ena# or*an#! an*

.#9er#!%a 'a!a 9enen")an %9e%#e%na "#.a! ')n*!#n .#(a!)!an7

Kara!"er#%"#! .an !(a%#=#!a%# %eba*#an '#!roba %e9er"# ba!"er# ber.a%ar!an

9a.a rea!%# en'a"#! a"a)9)n b#o!#'#a7 M#!roba .a9a" ")'b) 9a.a

bebera9a "#9e 'e.#a 'e'9ro.)!%# "#9e 'e"abo(#" "en")na an* .#.e"e!%#

.en*an #n"era!%# '#!roba .en*an rea*en "e% an* 'ana 'en*a%#(!an

9er)baan ;arna rea*en ?M)rra> 2004@7

S)a") en' a.a(a %)a") !a"a(#% b#o(o*#%7 a'9#r "#a9 re!a%# b#o!#'#a

.#!a"a(#% o(e en'7 En' 'er)9a!an !a"a(#% an* (eb# e=#%#en .ar#9a.a

!ebana!an !a"a(#% (abora"or#)' a"a) #n.)%"r#7 En' ,)*a 'e')n*!#n!an

%)a") %e(e!"#$#"a% 9erea!%#-9erea!%# .an %)a") 9en*en.a(#an (a,) rea!%#

an* "#.a! .#')n*!#n!an o(e !e(a% !a"a(#% (a#n7 Ke%9e%#=#!an en'

.#%ebab!an o(e ben")!na an* )n#! .an o(e *)*%-*)*)% 9o(ar ?a"a) non

9o(ar@ an* "ee.a9a" .a(a' %"r)!")r en' "er%eb)"7 Bebera9a en'


3/25

be!er,a ber%a'a %)a") !o=a!"or non 9ro"e#n> an* .a9a" ber)9a %ena;a

or*an#! 'a)9)n anor*an#! ?Len#n*er> 1++4@7

I.en"#=#!a%# ba%#( en"er#! %an*a" 9en"#n* .a(a' 'en*en.a(#!an #n=e!%# )%)%

.en*an 'ence*a !on"a'#na%# 9a%o!an 'a!anan .an a#r7 Ke(o'9o!ba!"er# an* .a9a" .#"e')!an .# %a()ran )%)% 'an)%#a .an 'a'a(#a an*

(eb# ren.a .#!(a%#=#!a%#!an %eba*a# an**o"a =a'#(Enterobacteriaeae7

an* "er'a%)! .a(a' !e()ar*a #n# a.a(a

1@ Pa"o*en %e9er"# an**o"a *enera Sa('one((a .an S#*e((a

2@ Se%e!a(# 9a"o*en %e9er"# an**o"a *enera Pro"e)% .an K(ab%#e((a

3@ an* nor'a( =(ora )%)% %e9er"# Escherichia an**o"a 'ar*a

.anEnterobacter> an* 'er)9a!an 9en.).)! saprophytic .ar# %a()ran

)%)%7

D#=eren%#a%# !e(o'9o! )"a'a En"erobac"er#aceae .a9a" .#ca9a# a"a% .a%ar

%#=a" b#o!#'#a .an rea!%# en'a"#! .# a.a9an %)b%"ra" "er"en")7 Ser# "e%

IMV#/> #n.o(> 'e"#(-'era> Vo*e%-Pre%!a)er> .an 9e'an=aa"an %#"ra" .a9a"

.#*)na!an )n")! #.en"#=#!a%# #n#7

1@ In.o(

Tr9"o9an 'er)9a!an a%a' a'#no e%en%#a( an* .a9a" 'en*a(a'#

o!%#.a%# .en*an cara !e*#a"an en'a"#! bebera9a ba!"er#7 Kon$er%#

"r#9"o=an 'en,a.# 9ro.)! 'e"abo(#! .# 'e.#a%# o(e en' Tr9"o9ana%e7

Me.#a #n# b#a%ana .#*)na!an .a(a' #n.e"#=#!a%# an* ce9a"7 Perben#an

#n.o( .#*)na!an )n")! 'e(#a" !e'a'9)an ba!"er# 'en.e*ra.a%# a%a'

a'#no "r#9"o=an %ecara en'a"#!7 a%#( ),# #n.o( an* .#9ero(e ne*a"#=

!arena "#.a! "erben")! (a9#%an ?c#nc#n@ ber;arna 'era ').a 9a.a

9er')!aan b#a!an> ar"#na ba!"er# #n# "#.a! 'e'ben")! #n.o( .ar#

"r9"o9an %eba*a# %)'ber !arbon> an* .a9a" .#!e"a)# .en*an

'ena'ba!an (ar)"an !o$a!%7 A%a' a'#no "r#9"o=an 'er)9a!an

!o'9onen a%a' a'#no an* (a' "er.a9a" 9a.a 9ro"e#n> %e#n**a a%a'

a'#no #n# .en*an ').a .a9a" .#*)na!an o(e '#!roor*an#%'e a!#ba"9en*)ra#an 9ro"e#n ?Vo(! .an ee(er> 1++3@7

2@ MR-VP

U,# MR Perben#an #n# .#*)na!an )n")! 'en.e"e!%# ba!"er# an* 'e'#(#!#

!e'a'9)an )n")! 'en*o!%#.a%# *()!o%a 'en*a%#(!an 9ro.)! a%a'

ber!on%en"ra%# "#n**# an* %"ab#( %e#n**a 'enebab!an 9 'e.#a ")r)n

#n**a .#ba;a 6>6 an* .#"an.a# .en*an a%#( 9o%#"#=> "er,a.# 9er)baan

;arna 'en,a.# 'era %e"e(a .#"a'ba!an Me"( Re.7 Ar"#na> ba!"er#


4/25

#n# 'en*aa%#(!an a%a' ca'9)ran ?'e"#(en *(#!on@ .ar# 9ro%e% =er'en"a%#

*()!o%a an* "er!an.)n* .a(a' 'e.#)' MR-VP ?Len#n*er> 1++4@7

U,# 'e"#( 'era ber"),)an )n")! 'en*e"a)# !e'a'9)an %)a") ba!"er#

)n")! 'en*a%#(!an a%a'-a%a' ca'9)ran> %e#n**a .a9a" 'n*)ba#n.#!a"o 'e"#( 'era 'en,a.# 'era7 Me.#)' an* .#*)na!an a.a(a

Me"#n re. an* .#"a'ba!an .en*an 'e"#( 'era> a%#( 9o%#"#= .#"an.a#

.en*an a.ana ;arna 'era7 Seba*a# con"o bebera9a ,en#% ba!"er#

'a'9) 'e'ben")! a%a' "e"a9# "#.a! c)!)9 bana! )n")! 'en*)ba

#n.#!a"or .an 9en)r)nan 9 %a'9a# 4>0 > 9a.a )')'na )n")!

'en*a'ba" !e(an*%)n*an #.)9 '#!roor*an#%'e7 Se.an*!an ba!"er#

%e9er"# E7 /o(# .a9a" 'e'ber#!an a%#( 9en*),#an 9o%#"#= !arena .a9a"

'en)r)n!an 9en*),#an 9o%#"#= .an .a9a" 'en)r)n!an 9 %a'9a# .# ba;a

6>47 Seba(#!na K(eb%#e((a aero*ene% 'en*a.a!an .e!arbo!%#(a%# .an

!on.en%a%# a%a' 9#r)$a" )n")! 'e'ben")! a%e"#('e"#(!arb#no(> %e#n**a

9 'en#n*!a"> .an b#(a .#"a'ba!an 'e"#( 'era ;arnana 'en,a.#

!)n#n* a( #n# berar"# a%#( 9en*),#an ne*a"#=7

3@ U,# Vo*e% Po%C)er

U,# $o*e% 9o%C)er ber"),)an )n")! 'en*e"a)# a9a!a %)a") ba!"er#

'a'9) 'en*a%#(!an a%e"o#n a"a) "#.a!7 Na')n> !ebera.aan a%e"o#n "#.a!

.a9a" .##.en"#=#!a%#> an* .a9a" .##.en"#=#!a%# ana(a !ebera.aan 2>3

b)"ana.#o(7 Da(a' ),# #n# .#*)na!an 'e.#)' MRVP ? Me"#( Re. Vo*e%

Po%C)er@> an* .##!)"# .en*an 9ena'baan rea*en ber)9a KO .an -

na="o(7 a%#( 9o%#"#= .#"an.a# .en*an a.ana ;arna 9#n! !e'eraan7

A%e"o#n 'er)9a!an %ena;a an"ara an* "erben")!.a(a' 9ro%%

'e"abo(#%'e !arbo#.ra"7 A%e"o#n .a(a' (#n*!)n*an an* 'en*an.)n*

9o"a%#)' #.ro!%#.a .an ).ara a!an "ero!%#.a%# 'en,a.# %ena;a 2>3

b)"ana.#o(7 Sena;a #n# .en*an a(9a-na="o( a!an 'en*a%#(!an ;arna

'era7

Den*an a%#( ne*a"#=> !arena "#.a! "erben")! ;arna 'era 9a.a 'e.#)'

%e"e(a .#"a'ba!an -na9"o( .an KO> ar"#na a%#( a!#r =er'en"a%#

ba!"er# #n#b)!an a%e"#( 'e"#( !arb#no( ?a%e"o(#n@ ?Vo(! .an ee(er> 1++3@7

6@ Simmons Citrate

Perben#an #n# .#*)na!an )n")! 'e(#a" !e'a'9)an or*an#%'e en"er#!

ber.a%ar!an !e'a'9)an 'e'=er'en"a%# %#"ra" %eba*a# %)'ber !arbon7

Perben#an Simmons Citrate #n# 'en*an.)n* #n.#!a"or b#r) bro'"#'o(

an* a!an ber)ba 'en,a.# b#r) 9a.a rea!%# 9o%#"#= .an "e"a9 #,a) ,#!a

rea!%# ne*a"#= ?Vo(! .an ee(er> 1++3@7

4@ U,# TSI


5/25

U,# TSI ber"),)an )n")! 'en*#.en"#=#!a%# ba!"er# an* bera%a( .ar# !e(a%

en"erobac"er#aceae7 U,# #n# b#a%a ,)*a .#*)na!an )n")! 'e'be.a!an *ra'

ne*a"#= an"ara an* 'a'9) 'en*!a"abo(#%'e *()!o%a> (a!"o%a> %)!ro%a>

.an 'a'9) 'e'beba%!an a%a' %)(=a"7 U,# #n# 'en**)na!an 'e.#)' TSI

.an #n.#!a"or 'e"#( 'era7 a%#( 9o%#"#= .#"an.a# .en*an ')nc)(na ;arna

!)n#n* .an 'era7 arna !)n#n* ')nc)( !arena a.ana =er'en"a%# ba!"er#

"era.a9 *()!o%a> %)!ro%a> a"a)9)n (a!"o%a .a(a' !on%en"ra%# "#n**#

%e.an*!an .a(a' !on%en"ra%# *)(a an* ren.a ana na'9a! ;arna

'era7 Se.an*!an a%#( 9o%#"#= a.ana 2S .#"an.a# .en*an a.ana ;arna

#"a'7

IV7 ALAT DAN BAAN

A71 U,# Pro.)!%# In.o(

A(a" Baan

3 Tab)n* .en*an 2 "ab)n*

'e.#)' !a(.) "r9"o9an 1

.an 1 "ab)n* 'e.#)' !a(.)

"r9"on 1 F *()!o%a 1

K)(")r b#a!an ba!"er#Escherichia coli,

Bacillus cereus,.an ba!"er#a A %er"a

ba!"er# B

P#9e" Me.#)' !a(.) "r9"o9an 1 .a(a' 2

"ab)n*

Me.#)' !a(.) "r9"on F *()!o%a 1

Lar)"an Ko$ac %e*ar

A72 Rea!%# Me"#( Mera

A(a" Baan

3 Tab)n* .en*an 'e.#)'

!a(.) MR-VP



ba!"er# B

Pe'ba!ar b)n%en Me.#)' !a(.) MR-VP .a(a' 3 "ab)n*

:ar)' #no!)(a%# Rea*en 'e"#( 'era

A73 Rea!%# Vo*)%-Pro%!a)er

A(a" Baan


!a(.) MR-VP



ba!"er# B

Pe'ba!ar b)n%en Me.#)' !a(.) MR-VP .a(a' 3 "ab)n*

:ar)' #no!)(a%# Rea*en Barr#" ? a(=a na="o( .an (ar)"an


6/25

KO 60 an* 'en*an.)n* 0>33

!rea"#n @

A76 Pen**)naan S#"ra"

A(a" Baan


A*ar '#r#n* S#''on% %#"ra"



ba!"er# B

Pe'ba!ar b)n%en Me.#)' a*ar '#r#n* S#''on% %#"ra"

.a(a' 3 "ab)n*:ar)' #no!)(a%#

B7 U,# A*ar Tr#9(e S)*ar Iron ?TSI@

A(a" Baan

Tab)n* .en*an 'e.#)' a*ar

'#r#n* TSI



ba!"er# B

Pe'ba!ar b)n%en Me.#)' a*ar '#r#n* TSI .a(a'

"ab)n*:ar)' #no!)(a%#

V7 ASIL PENGAMATAN

Tabe( A a%#( Pen*a'a"an U,# IMV#/ ? U,# Pro.)!%# In.o( @

No

7

a%#( Pen*a'a"an Ke"eran*an

1

Ga'bar A71 a%#( 9ercobaan

Ba!"er#Bacillus9a.a U,#

Pro.)!%# In.o( .a(a' Me.#)'

!a(.) "r9"o9an 1 F Lar)"an

Ko$ac

Tan**a( 9en*a'a"an 28

O!"ober 2014

S)'ber 'e.#a K)(")r B#a!an

Bacillus

Me.#a "),)an Me.#)' !a(.)

"r9"o9an 1

Ba!"er#Bacillus

D)ra%# In!)ba%# 68 ,a'Kara!"er#%"#!Echinulate

Per)baan Ter(#a"

9er")'b)an ba!"er# Bacillus

ber;arna 9)"# .a(a' 'e.#a

.an "er.a9a" c#nc#n ber;arna

'era 9a.a ba*#an a"a%

%)%9en%# ba!"er#

S)'ber =o"o !e(o'9o! 4

2 Tan**a( 9en*a'a"an 28


7/25


Ba!"er#A9a.a U,# Pro.)!%#

In.o( .a(a' Me.#)' !a(.)

"r9"o9an 1 F Lar)"an Ko$ac

O!"ober 2014


A

Me.#a "),)an Me.#)' !a(.)

"r9"o9an 1 Ba!"er#A

D)ra%# In!)ba%# 68 ,a'

Kara!"er#%"#! -

Per)baan Ter(#a"

9er")'b)an ba!"er# A




%)%9en%# ba!"er#

S)'ber =o"o !e(o'9o! 437


Ba!"er#B9a.a U,# Pro.)!%#

In.o( .a(a' Me.#)' !a(.)



O!"ober 2014


B

Me.#a "),)an Me.#)' !a(.)

"r9"o9an 1

Ba!"er#B

D)ra%# In!)ba%# 68 ,a'

Kara!"er#%"#! -

Per)baan Ter(#a"9er")'b)an ba!"er# B

ber;arna !)n#n* .a(a' 'e.#a



%)%9en%# ba!"er#


6



O!"ober 2014S)'ber 'e.#a K)(")r B#a!an

E.coli

Me.#a "),)an Me.#)' !a(.)

"r9"o9an 1

Ba!"er#E.coli

D)ra%# In!)ba%# 68 ,a'

Kara!"er#%"#! -

Per)baan Ter(#a"

9er")'b)an ba!"er# E.coli



8/25

Ba!"er#E.coli9a.a U,# Pro.)!%#

In.o( .a(a' Me.#)' !a(.)




%)%9en%# ba!"er#


4


Ba!"er#A9a.a U,# Pro.)!%#

In.o( .a(a' Me.#)' !a(.)

"r9"on 1 F *()!o%a 1


O!"ober 2014


A

Me.#a "),)an Me.#)' !a(.)

"r9"on 1

Ba!"er# A

D)ra%# In!)ba%# 68 ,a'

Kara!"er#%"#! -

Per)baan Ter(#a"

9er")'b)an ba!"er# Bacillusber;arna 9)"# .a(a' 'e.#a



%)%9en%# ba!"er#



Ba!"er#Bacillus9a.a U,#

Pro.)!%# In.o( .a(a' Me.#)'

!a(.) "r9"on 1 F *()!o%a 1


O!"ober 2014


BacillusMe.#a "),)an Me.#)' !a(.)

"r9"on 1

Ba!"er#Bacillus

D)ra%# In!)ba%# 68 ,a'

Kara!"er#%"#!Echinulate

Per)baan Ter(#a"




'era 9a.a ba*#an a"a%%)%9en%# ba!"er#



9/25

5


Ba!"er#B9a.a U,# Pro.)!%#

In.o( .a(a' Me.#)' !a(.)

"r9"on 1 F *()!o%a 1


O!"ober 2014


B

Me.#a "),)an Me.#)' !a(.)

"r9"on 1

Ba!"er#B

D)ra%# In!)ba%# 68 ,a'

Kara!"er#%"#! -

Per)baan Ter(#a"

9er")'b)an ba!"er# B

ber;arna !)n#n* (eb# 9).ar

.a(a' 'e.#a .an "er.a9a"

c#nc#n ber;arna !)n#n* 9a.a

ba*#an a"a% %)%9en%# ba!"er#


8


Ba!"er#E.coli9a.a U,# Pro.)!%#

In.o( .a(a' Me.#)' !a(.)

"r9"on 1 F *()!o%a 1


O!"ober 2014


E.coli

Me.#a "),)an Me.#)' !a(.)

"r9"on 1

Ba!"er#E.coli

D)ra%# In!)ba%# 68 ,a'

Kara!"er#%"#! -Per)baan Ter(#a"




!)n#n* 9a.a ba*#an a"a%

%)%9en%# ba!"er#


Tabe( B a%#( Pen*a'a"an U,# IMV#/ ? Rea!%# Me"#( Mera @

No a%#( Pen*a'a"an Ke"eran*an


10/25

7

1

Ga'bar B71 a%#( 9ercobaanBa!"er#A9a.a Rea!%# Me"#(

'era .a(a' Me.#)' !a(.) MR-

VP


O!"ober 2014


AMe.#a "),)an Me.#)' !a(.)

MR-VP

Ba!"er#A

D)ra%# In!)ba%# 68 ,a'

Kara!"er#%"#! -

Per)baan Ter(#a"

9er")'b)an ba!"er# A .a(a'

'e.#a .an "er.a9a" 9er)baan

;arna ber;arna 'era 9a.a

%e"en*a ba*#an a"a% 'e.#a

!a(.) MR-VP


2

Ga'bar B72 a%#( 9ercobaan

Ba!"er#Bacillus9a.a Rea!%#

Me"#( 'era .a(a' Me.#)'

!a(.) MR-VP


O!"ober 2014


Bacillus

Me.#a "),)an Me.#)' !a(.)

MR-VP

Ba!"er#BacillusD)ra%# In!)ba%# 68 ,a'


Per)baan Ter(#a"



9er)baan ;arna ber;arna

'era 9a.a %e9er"#*a ba*#an

a"a% 'e.#a !a(.) MR-VP



11/25

3


Ba!"er#B9a.a Rea!%# Me"#(

'era .a(a' Me.#)' !a(.) MR-

VP


O!"ober 2014


B

Me.#a "),)an Me.#)' !a(.)

MR-VP

Ba!"er#B

D)ra%# In!)ba%# 68 ,a'

Kara!"er#%"#! -

Per)baan Ter(#a"

9er")'b)an ba!"er# B .a(a'

'e.#a .an "er.a9a" bana!

%)%9en%# ber;arna #"a' 9a.a


.an ba*#an .a%ar 'e.#a !a(.)

MR-VP


6


Ba!"er#E.coli9a.a Rea!%# Me"#(

'era .a(a' Me.#)' !a(.) MR-

VP


O!"ober 2014


E.coli

Me.#a "),)an Me.#)' !a(.)

MR-VP

Ba!"er#E.coli

D)ra%# In!)ba%# 68 ,a'Kara!"er#%"#! -

Per)baan Ter(#a"



%e.#!#" %)%9en%# ber;arna

#"a' 9a.a ba*#an a"a%

%)%9en%# ba!"er# .an ba*#an

.a%ar 'e.#a !a(.) MR-VP


Tabe( / a%#( Pen*a'a"an U,# IMV#/ ? Rea!%# Vo*)%-Pro%!a)er @

No

7



12/25

1

Ga'bar /71 a%#( 9ercobaan

Ba!"er#A9a.a Rea!%# Vo*)%-

Pro%!a)er .a(a' Me.#)' !a(.)

MR-VP F Rea*en Barr#"


O!"ober 2014


A

Me.#a "),)an Me.#)' !a(.)

MR-VP

Ba!"er#A

D)ra%# In!)ba%# 68 ,a'

Kara!"er#%"#! -

Per)baan Ter(#a"


'e.#a ber;arna (eb# 9e!a"

.an "#.a! "er.a9a" 9er)baan

;arna a"a) rea!%# 9a.a 'e.#a

!a(.) MR-VP


2


Ba!"er#Bacillus9a.a Rea!%#

Vo*)%-Pro%!a)er .a(a' Me.#)'

!a(.) MR-VP F Rea*en Barr#"


O!"ober 2014


Bacillus

Me.#a "),)an Me.#)' !a(.)

MR-VP

Ba!"er#Bacillus

D)ra%# In!)ba%# 68 ,a'


Per)baan Ter(#a"



bana! *e(e'b)n* ).ara 9a.a


a"a) 'e.#a !a(.) MR-VP


3 Tan**a( 9en*a'a"an 28O!"ober 2014


B

Me.#a "),)an Me.#)' !a(.)

MR-VP

Ba!"er#B

D)ra%# In!)ba%# 68 ,a'

Kara!"er#%"#! -

Per)baan Ter(#a"



13/25


Ba!"er#B9a.a Rea!%# Vo*)%-

Pro%!a)er .a(a' Me.#)' !a(.)

MR-VP F Rea*en Barr#"

'e.#a 'en,a.# (eb# 9e!a" .an

"#.a! "er.a9a" 9er)baan

;arna .an rea!%# 9a.a 'e.#a

!a(.) MR-VP


6


Ba!"er#E.coli9a.a Rea!%#

Vo*)%-Pro%!a)er .a(a' Me.#)'

!a(.) MR-VP F Rea*en Barr#"


O!"ober 2014


E.coli

Me.#a "),)an Me.#)' !a(.)

MR-VP

Ba!"er#E.coli

D)ra%# In!)ba%# 68 ,a'

Kara!"er#%"#! -

Per)baan Ter(#a"9er")'b)an ba!"er# E.coli


%e.#!#"S 9er)baan ;arna

ber;arna (eb# 9).ar .an

rea!%# 9a.a 'e.#a !a(.) MR-

VP


Tabe( D a%#( Pen*a'a"an U,# IMV#/ ? Pen**)naan S#"ra" @

No

7


1

Ga'bar D71 a%#( 9ercobaan

Ba!"er#A9a.a Pen**)naan S#"ra"

.a(a' Me.#)' a*ar '#r#n*

S#''on% %#"ra"


O!"ober 2014


A

Me.#a "),)an Me.#)' a*ar

'#r#n* S#''on% S#"ra"

Ba!"er#AD)ra%# In!)ba%# 68 ,a'

Kara!"er#%"#! -

Per)baan Ter(#a"


'e.#a ber;arna 9)"# 9a.a

%e9an,an* a%#( #no!)(a%#

streak .anstab %er"a "er.a9a"

9er)baan ;arna an*

.o'#nan ber;arna b#r) cera

9a.a %e9an,an* a%#( #no!)(a%#


14/25

streak.anstab


2


Ba!"er#Bacillus9a.a

Pen**)naan S#"ra" .a(a'

Me.#)' a*ar '#r#n* S#''on%

%#"ra"


O!"ober 2014

S)'ber 'e.#a K)(")r B#a!anBacillus


'#r#n* S#''on% S#"ra"

Ba!"er#Bacillus

D)ra%# In!)ba%# 68 ,a'


Per)baan Ter(#a"



%e9an,an* a%#( #no!)(a%#streak .anstab %er"a "er.a9a"

9er)baan an* %e.#!#"

.o'#nan ber;arna b#r) 9a.a

ba*#an "en*a a%#( #no!)(a%#

streak.anstab


3


Ba!"er#B9a.a Pen**)naan S#"ra"

.a(a' Me.#)' a*ar '#r#n*S#''on% %#"ra"


O!"ober 2014


B


'#r#n* S#''on% S#"ra"

Ba!"er#B

D)ra%# In!)ba%# 68 ,a'

Kara!"er#%"#! -

Per)baan Ter(#a"



%e9an,an* a%#( #no!)(a%#


9er)baan ;arna an* %an*a"

.o'#nan ber;arna b#r) 9a.a

%e9an,an* a%#( #no!)(a%#

streak.anstab #n**a ba*#an

.a%ar 'e.#a a*ar '#r#n*

S#''on% %#"ra"



15/25

6


Ba!"er#E.coli9a.a Pen**)naan

S#"ra" .a(a' Me.#)' a*ar '#r#n*

S#''on% %#"ra"


O!"ober 2014


E.coli


'#r#n* S#''on% S#"ra"

Ba!"er#E.coli

D)ra%# In!)ba%# 68 ,a'

Kara!"er#%"#! -

Per)baan Ter(#a"


.a(a' 'e.#a ber;arna 9)"#

9a.a %e9an,an* a%#( #no!)(a%#


9er)baan ;arna an* %an*a"

.o'#nan "e"a9# 'a%#

.o'#nan 9a.a ba!"er# B

ber;arna b#r) 9a.a %e9an,an*

a%#( #no!)(a%#streak.anstab

#n**a ba*#an .a%ar 'e.#a

a*ar '#r#n* S#''on% %#"ra"


Tabe( E a%#( Pen*a'a"an U,# A*ar Tr#9(e S)*ar ? TSI @

No

7


1

Ga'bar E71 a%#( 9ercobaan

Ba!"er#A9a.a U,# A*ar Tr#9(e

S)*ar ? TSI @ .a(a' Me.#)' a*ar

'#r#n* TSI


O!"ober 2014


A


'#r#n* TSI

Ba!"er#AD)ra%# In!)ba%# 68 ,a'

Kara!"er#%"#! -

Per)baan Ter(#a"



%e9an,an* a%#( #no!)(a%#

streak.anstab an* "er9)")%-

9)")% an"ar !o(on# %er"a

bana! "er.a9a" *e(e'b)n*

*a% 9a.a ba*#an '#r#n* 9a(#n*


16/25

.a%ar 'e.#a a*ar TSI %er"a

%e.#!#" .aera a*ar an*

"eran*!a" 'en,a.# r)an* ).ara


2


Ba!"er#Bacillus9a.a U,# A*ar

Tr#9(e S)*ar ? TSI @ .a(a'

Me.#)' a*ar '#r#n* TSI


O!"ober 2014


Bacillus


'#r#n* TSI

Ba!"er#Bacillus

D)ra%# In!)ba%# 68 ,a'


Per)baan Ter(#a"

9er")'b)an ba!"er# Bacillus.a(a' 'e.#a ber;arna 9)"#

9a.a %e9an,an* a%#( #no!)(a%#

streak .an stab an* 'a%#

)") %er"a "er.a9a" *e(e'b)n*

*a% 9a.a ba*#an '#r#n* 9a(#n*

.a%ar 'e.#a a*ar TSI %er"a

"er.a9a" bana! .aera a*ar

an* "eran*!a" 'en,a.# r)an*

).araS)'ber =o"o !e(o'9o! 4

3


Ba!"er#B9a.a U,# A*ar Tr#9(e

S)*ar ? TSI @ .a(a' Me.#)' a*ar

'#r#n* TSI


O!"ober 2014


B


'#r#n* TSI

Ba!"er#B

D)ra%# In!)ba%# 68 ,a'

Kara!"er#%"#! -

Per)baan Ter(#a"



%e9an,an* a%#( #no!)(a%#

streak .an stab an* 'a%#

)") %er"a "er.a9a" bana!

*e(e'b)n* *a% 9a.a ba*#an

'#r#n* 9a(#n* .a%ar 'e.#a

a*ar TSI7 Ter.a9a" 9er)baan

;arna ber;arna #"a' *e(a9


17/25

9a.a ba*#an .a%ar #n**a

ba*#an .a%ar '#r#n* .a(a'

'e.#)' a*ar TSI> !o(on#

ba!"er# "er9#%a-9#%a


6


Ba!"er#E.coli9a.a U,# A*ar

Tr#9(e S)*ar ? TSI @ .a(a'

Me.#)' a*ar '#r#n* TSI


O!"ober 2014


E.coli


'#r#n* TSI

Ba!"er#E.coli

D)ra%# In!)ba%# 68 ,a'

Kara!"er#%"#! -

Per)baan Ter(#a"9er")'b)an ba!"er# E.coli

.a(a' 'e.#a ber;arna 9)"#

9a.a %e9an,an* a%#( #no!)(a%#

streak .an stab an*

!o(on#na "er9#%a-9#%a %er"a

"er.a9a" bana! *e(e'b)n*

*a% 9a.a ba*#an '#r#n* 9a(#n*

.a%ar 'e.#a a*ar TSI7

Ter.a9a" 9er)baan ;arnaber;arna ,#n**a (eb# 9).ar

9a.a ba*#an .a%ar #n**a

ba*#an .a%ar '#r#n* .a(a'

'e.#)' a*ar TSI


VI7 ANALISIS

A7 U,# Pro.)!%# In.o(

Pa.a Percobaan ),# 9ro.)!%# #n.o( 9er"a'a !a(# .#(a!)!an #no!)(a%# .ar# !)(")r

b#a!an ba!"er#A, B, Bacillus, .an E.coli !e .a(a' 'e.#)' !a(.) "r9"o9an 1

.an 'e.#)' !a(.) "r9"on 1 F *()!o%a 1 %ecara a%e9"#! a*ar a%#( 9ercobaan

an* .#.a9a" "#.a! "er!on"a'#na%# ba!"er# (a#n "er")"a'a .# ).ara7 Sena;a #n.o(


18/25

%en.#r# %eba*a# c#r# !a% .ar# ba!"er# #n"e%"#na( a"a) ba!"er# )%)% an* .#a%#(!an

.ar# .e*ra.a%# "r#9"o=an> %)a") a%a' a'#no e%en%#a(> o(e a.ana en'

"r#9"o=ana%e an* .#ben")! ba!"er#7 Da(a' 'e.#)' .#*)na!an 'e.#)' !a(.)

"r9"on 1 !arena !an.)n*an "r#9"o=anna an* "#n**# an* .#9ero(e .ar# !a%e#n

.en*an 9encernaan 9an!rea"#!7 a( #n# .#.a%ar!an 9a.a !e'a'9)an ba!"er#*o(on*an #n"e%"#na( a"a) En"erobac"er#aceae .a(a' 'en*)ra#!an "r#9"o=an )n")!

'en*a%#(!an #n.o(e a!#ba" en' an* .#'#(#!# ba!"er# "er%eb)" ? "r#9"o=ana%e @7

Se"e(a .#(a!)!)!an #n!)ba%# %e(a'a 26-68 ,a' 9a.a %)) 35 .era,a" ce(c#)%

!)(")r ba!"er# A, B, Bacillus, .an E.coli %e#n**a ")'b) 9a.a 'e.#)' !a(.)

"r9"o9an 1 .an "r9"on 1 F *()!o%a 1 7 Ke').#an .#(a!)!an 9ena'baan

(ar)"an !o$ac 10 "e"e% 'e(a()# .#n.#n* "ab)n* a*ar "#.a! 'en*)ran*# ,)'(a

(ar)"an !o$ac an* "er(ar)"7 In.o( an* "erben")! a!an ber;arna 'era %aa"

.#"e"e%# rea*en !o$ac a"a) Er(#c an* 'en*an.)n* 9-.#'e"#(ben&a(.e#.7 Rea!%#

ber%#=a" 9o%#"#= a9ab#(a %ena;a #n# 'en*a%#(!an %ena;a 9-

.#'e"#(a'#noben&a(.e#. an* "#.a! (ar)" .a(a' a#r .an 'e'ben")! ;arna 'era

.# 9er')!aan 'e.#)'7

B7 Rea!%# Me"#( Mera

Pa.a Percobaan Rea!%# 'e"#( 'era 9er"a'a !a(# .#(a!)!an #no!)(a%# .ar# !)(")r

b#a!an ba!"er# A, B, Bacillus, .an E.coli !e .a(a' 'e.#)' !a(.) MR-VP %ecara

a%e9"#! a*ar a%#( 9ercobaan an* .#.a9a" "#.a! "er!on"a'#na%# ba!"er# (a#n

"er")"a'a .# ).ara7 Da(a' !a(.) MR-VP 'en*an.)n* *()!o%a 'ono%a!ar#.a

.#"a'ba a#r .ar# !e(o'9o! e!%o%a %e9er"# *()!o%a an* 'er)9a!an %)'berener*# )"a'a ba*# ba!"er# )%)% .an a!an 'en*a%#(!an a%a' .#an"arana a%a'

(a!"a"> a%a' a%e"a"> .an a%a' =or'a"> 9ro.)! a!#r an* "er,a.# ber$ar#a%#

"er*an")n* 9a.a en' an* .#a%#(!an '#!roor*an#%'e )n")! 'en*e!%"ra!%#na7

Se"e(a .#(a!)!an #no!)(a%# (a() .##n!)ba%# %e(a'a 26-68 ,a' 9a.a %)) 35

.era,a" ce(c#)% %e#n**a "er(#a" ba!"er#A, B, Bacillus, .an E.coli an* ")'b)

.en*an $ar#a%# ;arna 'en.e!a"# 9e!a"7 Ke').#an .#"a'ba!an 4 "e"e% 'e"#(

'era 9a.a 9er')!aan %)%9en%# 'e(a()# 9#n**#r "ab)n* a*ar "#.a! 'en*)ran*#

,)'(a (ar)"an 'e"#( 'era an* .#(ar)"!an7 Me"#( 'era %eba*a# #n.#!a"or rea*en

97 Pa.a 9 %e!#"ar 6 a!an 'en)n,)!an "erben")!na c#nc#n 'era an*'en*#n.#!a%#!an a%#( an* 9o%#"#= 7 Se.an*!an 9a.a 9 > ;a(a)9)n 'a%# a.a

a%a' "e"a9# .en*an !on%en"ra%# #on #.ro*en an* %e.#!#"> a!an 'en*)ba

#n.#!a"or 'en,a.# !)n#n*> !arena "erben")!na a%e"#('e"#( !arb#no(7

/7 Rea!%# Vo*)%-Pro%!a)er

Pa.a Percobaan Rea!%# Vo*en-Pro%!a)er 9er"a'a !a(# .#(a!)!an #no!)(a%# .ar#

!)(")r b#a!an ba!"er#A, B, Bacillus, .an E.coli !e .a(a' 'e.#)' !a(.) MR-VP

%ecara a%e9"#! a*ar a%#( 9ercobaan an* .#.a9a" "#.a! "er!on"a'#na%# ba!"er# (a#n


19/25

"er")"a'a .# ).ara7 Da(a' !a(.) MR-VP 'en*an.)n* *()!o%a 'ono%a!ar#.a

.#"a'ba o!%#*en beba% %eba*a# ),# )n")! 'en.e"e!%# !e'a'9)an bebera9a

or*an#%'e )n")! 'en*a%#(!an 9ro.)! %ena;a b)!an a%a' %e9er"#

a%e"#'e"#(!arb#no( .ar# a%#( 9e'ecaan a%a' a%e"a" .an %eba*a# a%#( a!#r .ar#

=er'en"a%# *()!o%a a"a) a%a' or*an#! (a#nna7 Se"e(a .#(a!)!an #no!)(a%# (a()

.##n!)ba%# %e(a'a 26-68 ,a' 9a.a %)) 35 .era,a" ce(c#)% %e#n**a "er(#a" ba!"er#

A, B, Bacillus, .an E.coli an* ")'b) .en*an $ar#a%# ;arna 'en.e!a"# 9e!a"7

Ke').#an .#"a'ba!an 1 '( (ar)"an a(=a na="o( 9a.a 9er')!aan %)%9en%# 'e(a()#

9#n**#r "ab)n* a*ar "#.a! 'en*)ran*# ,)'(a (ar)"an a(=a na="o( an* .#(ar)"!an7

Ke').#an %e*era .#"a'ba!an 1 '( (ar)"an KO 60 .en*an !rea"#n 0>3 .an

ca'9)r!an .en*an %e'9)rna> (a() .#")n**) 4-30 'en#" a*ar Rea*en Barr#"

"er%eb)" .a9a" berea!%# %e'9)rna7 De"e!%# a%e"#'e"#(!arb#no( .#9er()!an !arena

9ro.)! a!#r #n# .a9a" "ero!%#.a%# 'en,a.# %ena;a .#a%e"#(7 D#a%e"#( a!an

'e'ben")! .en*an !a"a(#% a(=ana="o( .an *r)9 *)an#.#n an* a.a 9a.a 9e9"on .ar#'e.#)' MR-VP7 a%#(na a.a(a !o'9(e!% ;arna 'era ').a 9a.a 'e.#)'

an* berar"# a%#( 9o%#"#=7

D7 Pen**)naan S#"ra"

Pa.a Percobaan Pen**)naan %#"ra" 9er"a'a !a(# .#(a!)!an #no!)(a%# .ar# !)(")r

b#a!an ba!"er#A, B, Bacillus, .an E.coli !e .a(a' 'e.#)' a*ar '#r#n* S#''on%

%#"ra" %ecara a%e9"#! a*ar a%#( 9ercobaan an* .#.a9a" "#.a! "er!on"a'#na%#

ba!"er# (a#n "er")"a'a .# ).ara7 Ino!)(a%# .#(a!)!an .en*an cara stab.anstreak

a( #n# .#(a!)!an a*ar a%#( 9en*a'a"an nan"# .a9a" .#(#a" ,)*a =a!"or !eb)")an%#"ra" .an #.en"#=#!a%# ben")! !o(on# ba!"er#7 U,# #n# .#(a!)!an )n")! 'en*e"a)#

!e'a'9)an '#!roor*an#%'e 'en**)na!an %#"ra" %eba*a# %)'ber !arbon )n")!

'en*a%#(!an ener*#> .#%aa" "#.a! a.ana *()!o%a a"a) (a!"o%a )n")! =er'en"a%#7

Ke'a'9)an #n# .#.a%ar!an a.ana en' %#"ra" 9er'ea%e an* 'e'=a%#(#"a%#

"ran%9or" %#"ra" .# .a(a' %e(7 Se"e(a .#(a!)!an #no!)(a%# (a() .##n!)ba%# %e(a'a

26-68 ,a' 9a.a %)) 35 .era,a" ce(c#)% %e#n**a "er(#a" ba!"er# A, B, Bacillus,

.an E.coli an* ")'b) .en*an ')nc)(na !o(on# ba!"er# 9a.a *ore%an streak

'a)9)n stab. Kea.#ran %o.#)' !arbona" an* .#a%#(!an .ar# !arbon.#o!%#.a

%eba*a# a%#( .ar# a%a' o!%a(oa%e"a" .an a%e"a" an* 'e'b)a" #n.#!a"ror

bro'"#'o( b#r) an* 'er)9a!an !o'9onen 'e.#)' 'en*)ba 'e.#)' .ar#

;arna #,a) 'en,a.# b#r) %e#n**a .a9a" .##.en"#=#!a%# .en*an a.ana

9er")'b)an 9a.a 9er')!aan a*ar '#r#n* .an 9a.a a%#( stab .en*an ;arna

'e.#)' b#r) !e)n*)an berar"# a%#( 9o%#"#= ? S#"ra"@ .an a%#( ne*a"#= ,#!a

'e'9er(#a"!an 9er")'b)an .an 'e.#)' "e"a9 ber;arna #,a)7

E7 U,# A*ar Tr#9(e S)*ar Iron


20/25

Pa.a Percobaan U,# a*ar "r#9(e %)*ar #ron 9er"a'a !a(# .#(a!)!an #no!)(a%# .ar#

!)(")r b#a!an ba!"er#A, B, Bacillus, .an E.coli !e .a(a' 'e.#)' a*ar '#r#n* TSI

%ecara a%e9"#! a*ar a%#( 9ercobaan an* .#.a9a" "#.a! "er!on"a'#na%# ba!"er# (a#n

"er")"a'a .# ).ara7 Ino!)(a%# .#(a!)!an .en*an cara stab .an streak a( #n#

.#(a!)!an a*ar a%#( 9en*a'a"an nan"# .a9a" .#(#a" ,)*a =a!"or 9ro.)!%# *a% .an

#.en"#=#!a%# ben")! !o(on# ba!"er#7 U,# #n# .#(a!)!an )n")! 'e'be.a!an !e(o'9o!

a"a) *en)% an* 'er)9a!an =a'#(#Enterobacteriaceae> an* 'er)9a!an ba!"er#

*ra' ne*a"#= berben")! ba"an* an* 'a'9) 'e'=er'en"a%#!an *()!o%a .an

'en*a%#(!an a%a'7 Perbe.aan #n# .#.a%ar!an 9a.a 9o(a =er'en"a%# !arbo#.ra"

.an 9ro.)!%# #.ro*en %)(=#.a o(e ber'aca' !e(o'9o! or*an#%'e #n"e%"#na(7

Se"e(a .#(a!)!an #no!)(a%# (a() .##n!)ba%# %e(a'a 26-68 ,a' 9a.a %)) 35

.era,a" ce(c#)% %e#n**a "er(#a" ba!"er#A, B, Bacillus, .an E.coli an* ")'b)

.en*an ')nc)(na !o(on# ba!"er# 9a.a *ore%anstreak 'a)9)nstab. Da(a' a*ar

TSI #n# ber#%# (a!"o%a .an %)!ro%a .en*an !on%en"ra%# 1 .an *()!o%a 1 an*a!an .#a'a"# 9en**)naana7 In.#!a"or =eno( 'era .#"a'ba!an .a(a' 'e.#)'

)n")! 'en.e"e!%# =er'en"a%# !arbo#.ra" an* .##n.#!a%#!an .en*an 9er)baan

;arna 'e.#)' .ar# ,#n**a-'era 'en,a.# !)n#n* b#(a a.a a%a' "erben")!7 Pa.a

9er')!aan a*ar '#r#n* ba%a ?'era@ .an 9a.a .a%ar "ab)n*> a*ar .a(a' !on.#%#

a%a' ?!)n#n*@ .en*an a"a) "an9a 9ro.)!%# *a% ?'e'eca a*ar 9a.a .a%ar "ab)n*@

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S)'ber Pre%en"a%# Dr7Bar"#>Environmental Microbiology

-Laboratory Manual-

prepare !or Environmental Microbiology "# Biochemical Activity o!

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:#!a .#ban.#n*!an .en*an (#"era")r a%#( 9ercobaan an* !a'# (a!)!an %a'a

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9a.a "#n*!a" !on%en"ra%# a%a' .an ba%a %er"a !)an"#"a"#= *a% .an 2S an* "er.a9a"9a.a 'a%#n*-'a%#n* 9ercobaan7

VII7 KESIMPULAN

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VIII7 DATAR PUSTAKA

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No$e'ber 2014 9)!)( 22700

Pre%en"a%# Dr7Bar"#7 Environmental Microbiology-Laboratory Manual-

prepare !or Environmental Microbiology "# Biochemical Activity o!

microorganism. ITB Ban.)n* 20107

Dr7Bar"# Se"#an# M)n"a(#= .!!> Mo.)( Pra!"#!)' M#!(#n*. ITB

Ban.)n* 2013

D;#,o%e9)"ro> D7 Da%ar-.a%ar M#!rob#o(o*#7 :a!ar"a D,a'ba"an720047

a()o7 Pen*an"ar M#!rob#o(o*#7 Ban.)n* Tar%#"o720047
https://www.academia.edu/9327419/Selihttps://www.academia.edu/9327419/Seli


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