modul hikmah 8

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SCIENCE SPM

MODULE 8

PAPER 1

1. The table below shows the temperature and pH some bacterial culture in a petri dish.

Table 1

Which petri dish was cloudier after two days?

A. PB. QC. RD. S

2. Active immunity is obtained when vaccine which injected into the blood stream

A. requires antibiotic for treatmentB. stimulates the body to produce white blood cellC. stimulates the body cells to produces antibodyD. requires serum containing antibody.

3. Algae are neither parasite nor saprophyte because they are

A. Do not carry out respiration.

B. Carry out photosynthesisC. Can live without doing any activityD. Can synthesize organic substances



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4. The diagram below shows a type of a microorganism.

Figure 1

How does this microorganism reproduce?

A. releasing spores

B. binary fissionC. buddingD. bacteriophage

5. Which of the following statement is not true about viruses?

A. contain deoxyribonucleic acidB. live as saprophyteC. do not carry out respirationD. the smallest microorganism

6. Which disease is spread by housefly?

A. DengueB. Cholera

C. MalariaD. Hepatitis B

7. Which an effective method to prevent ringworm?

A. injectionB. sterilization

C. vector controlD. personal hygiene



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8. Penicillin is an

A. antigenB. antisepticC. antitoxineD. antibiotic

9. The microorganism which helps in the digestion of cellulose in herbivores is

A. algaeB. fungiC. virusD. bacteria

10. Yeast is added to pineapple juice in a conical flask. The mixture is left for five days.The process that occurs is

A. fermentationB. esterificationC. oxydationD. distillation



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PAPER 2SECTION A + B

1. Figure 1 shows an experiment carried out to study the effect of pH on the growth ofmicroorganisms.

Figure 1

Three sterile test-tubes labeled A, B and C with different pH are placed in the dark placeat room temperature for two days. The observations are after two days.

a. What is the hypothesis of this experiment?

b. Which test-tube is the cloudiest?

c. Why do we need to sterilise the test-tubes, cotton wool and nutrient agar beforethe experiment?

d. State the manipulated variables of this experiment.

e. Base on this experiment, state the meaning of the microorganism(operational definition)



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2. Figure 2 shows an experiment carried out to study the effect of temperature on thegrowth of microorganisms.

Figure 2

Three sterile test-tubes A, B and C are placed at different location for two days. Theobservation are made and recorded in Table 1

Table 1

a. What is the hypothesis of this experiment?

b. State the constant variable in this experiment.

c. What inference can you make based on this experiment?

d. Why do foods stored in refrigerator last longer?

Test-tube Location Observation

A Refrigerator Still remains

B Dark place in laboratoryCloudy

C Incubator Still remains



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e. Does human body temperature suits the growth of bacteria?

3. Figure 3 shows the life cycle of the mosquito.

Figure 3

a. Name stage Q.

b. Why is the mosquitoes called vector?

c. Name two diseases can be spread by mosquitoes

i.

ii.

d. Suggest one ways to control mosquitoes at stage P.



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4. Figure 5 shows the life cycle of a housefly

Figure 4

a. Name the stage P and Q

P:

Q:

b. How can houseflies be controlled at stage Q?

c. Name one disease can be spread by houseflies.



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5. Figure 5 shows an experiment to study the effect of an antibiotic on bacterialgrowth.

Figure 5

Three antibiotic discs, A1, A2 and A3 with different concentrations are placed onnutrient agar surface. The petri dish is incubated inverted at 37oC. After two days, aclear area is formed around each disc. The diameter of the clear area is measuredand recorded in Table 2.

Antibiotic Disc Diameter of the clear area (cm)

A1 1.2

A2 0.6

A3 1.6

Table 2a. State one hypothesis for this experiment.

b. From this experiment, state the:

i. manipulated variable

ii. responding variable

iii. fixed variable

c. Why the petri dish should incubate inverted?



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d. Based on this experiment defined antibiotic operationally.

e. Name one disease which can be treated by using antibiotic.

6. Figure 6 shows the level of antibody.

Figure 6

a. Name the type of immunity shows in the diagram above.

b. Give one example of disease which can be prevented using this immunity.

c. Why should we need a second dose?



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7. Table 3 shows the results of an experiment to study the effects of temperature onbacterial growth.

Temperature/ 0C Size of bacteria colony after 24 hours

0 2

15 5

26 10

35 15

60 3

a. State one hypothesis that can be made from table 2

b.i. Based on Table 3, draw graph to show the relationship between the size of

bacterial colony and temperature.

ii. Base on the graph, state the optimum temperature for bacterial growth.

iii. Predict the size colony at 80oC.



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SECTION C

1. Study statement below:

a. Suggest one suitable hypothesis to investigate the above statement.b. Explain an out line of the experiment to test the hypothesis base on the following

criteria:i. Aim of experiment.ii. Identifying variablesiii. List of material and apparatusiv. Methodv. Tabulation of data

2. a.

State four differences between viruses and bacteria.

b.

Explain how the residents in the area overcome the problem caused by

mosquitoes. Your answer should include the following:Identify the problemClarification the problemSolving methodsChoose the best method and explain your choice.

3. Study the following statement.

You are given some sterile nutrient broth and bacterial culture.a) Suggest a hypothesis to investigate the above statement.b) Describe one experiment to test your hypothesis based on the following criteria.

i. aim of the experimentii. identifying the variablesiii. list of apparatus and materialiv. methodv. tabulation of data

Light can effect the growth and reproduction of bacteria

Dengue fever is cause by a virus whereas tuberculosis is caused by bacteria

The residents of Kampong Kuala Paya were caught off guard by the news of

boy in their neighbourhood being effected by dengue fever. The residents

decided to overcome the problem.

Temperature influences the growth of microorganism.



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MODULE 8 - ANSWERS

PAPER 11. C 2. C 3. B 4. A 5. B 6. B 7. D 8. D 9. D 10. A

PAPER 2

SECTION A + B

1.a. Neutral environment with pH is the most suitable for the growth of bacteria.b. Test-tube Ac. to ensure bacteria from outside do not interfere with the result of the experiment.d. pH valuee. Microorganism is shown by the cloudiest of nutrient agar.

2.a. room temperature is the most suitable temperature for the growth of themicroorganism.

b. type of bacteria/volume of nutrient agar/pHc. test-tube B is cloudy because the microorganism/bacteria is suitable for growth atroom temperature.d. microorganism cannot grow at very low temperature.e. yes, it does

3.a. pupaeb. mosquitoes carried pathogen.c. i. dengue fever ii. Malariad. spray insecticides.

4.a. pupae, larvaeb. cover food properly ; clean the garbage and sewagec. cholera

5.a. antibiotic can kill bacteriab. i. different concentrations of antibiotic.

ii. diameter of the clear areaiii. type of bacteria/ room temperature@ 370C

c. to prevent water vapour from interfere the result of the experiment.d. Antibiotic is shown by the diameter of the clear area.f. syphilis/gonorrhea.

6.a. artificial active immunityb. rubella/chickenpox….c. as a booster dose to increase the antibody level .d. substance(weakened/dead pathogens) that stimulate white blood cell to produceantibodies.



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7.a. the most suitable temperature for the growth of the microorganism/bacteria is 370C

b. i.

ii. 360C +_ 1

iii.0/zero/none

SECTION C

Criteria Explanation

i.Aim To study the effect of light to the growth andreproduction of bacteria.

ii. Identifying variables i. manipulated variables: light conditionsii. fix variables: same quantity of nutrientagar/same type of bacteria/samepH/temperature(room temperature).iii. responds variables: the growth ofbacteria(the cloudiness of nutrient agar)

iii.List material and apparatus a. bacterial culture, nutrient agar

b. two test-tube, sterile cotton wool



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vi. method

a. two test-tube labeled A and Bas shownabove is prepared.

b. 5 cm3 of nutrient agar and 1 cm3 ofBacillus Subtilis bacterial culture is put

into each test-tube.c. each test-tube is covered with sterile

cotton wool.d. test-tube A is placed in dark place and

test-tube B is placed near the window fortwo days.

e. The observation is recorded. in the tableafter two days.

v. Tabulation of data

Test-tube Observation

A

B



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SECTION C1.

a. bacteria can growth and reproduce in dark place



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3.

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