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LAMPIRAN 6

Uji Penetrasi APMS dan Permeabilitas MembranHasil Pengukuran Kadar APMS Pada Membran Kulit Utuh Replikasi 1waktu (menit)absorbankoreksi absorbankadar (ppm)koreksikadar setelah koreksi (ppm)Jumlah dalam 60 ml (g)Jumlah kumulatif persatuan luas (g/cm2)

00.04600.00000.00000.00000.00000.00000.0000

50.05400.00800.00000.00000.00000.00000.0000

100.06000.01400.00000.00000.00000.00000.0000

150.06800.02200.08330.00000.08335.00001.5924

200.06900.02300.09380.00280.09655.79171.8445

250.07600.03000.16670.00590.172610.35423.2975

300.09300.04700.34380.01150.355221.31256.7874

350.09600.05000.37500.02290.397923.87507.6035

400.10700.06100.48960.03540.525031.500010.0318

450.11200.06600.54170.05170.593435.604211.3389

600.13300.08700.76040.06980.830249.812515.8639

750.13200.08600.75000.09510.845150.708316.1492

900.16700.12101.11460.12011.234774.083323.5934

1200.19500.14901.40630.15731.563593.812529.8766

1500.20700.16101.53130.20421.7354104.125033.1608

1800.21500.16901.61460.25521.8698112.187535.7285

2100.24700.20101.94790.30902.2569135.416743.1263

2400.25900.21302.07290.37402.4469146.812546.7556

2700.33700.29102.88540.44313.3285199.708363.6014

3000.39800.35203.52080.53924.0601243.604277.5809

Kurva hubungan antara t vs jumlah kumulatif APMS yang berpenetrasi ke dalam larutan dapar pfosfat pH 70,05 tiap cm2 pada suhu 37 0,5 C pada membran kulit tikus utuh replikasi 1Hasil Pengukuran Kadar APMS Pada Membran Kulit Tikus Utuh Replikasi 2

waktu (menit)absorbankoreksi absorbankadar (ppm)koreksikadar setelah koreksi (ppm)Jumlah dalam 60 ml (g)Jumlah kumulatif persatuan luas (g/cm2)

00.04700.00000.00000.00000.00000.00000.0000

50.05500.00800.00000.00000.00000.00000.0000

100.06200.01500.00000.00000.00000.00000.0000

150.06700.02000.06250.00000.06253.75001.1943

200.07000.02300.09380.00210.09585.75001.8312

250.07700.03000.16670.00520.171910.31253.2842

300.09200.04500.32290.01080.333720.02086.3761

350.09600.04900.36460.02150.386123.16677.3779

400.11000.06300.51040.03370.544132.645810.3968

450.11200.06500.53130.05070.581934.916711.1200

600.12500.07800.66670.06840.735144.104214.0459

750.13200.08500.73960.09060.830249.812515.8639

900.15000.10300.92710.11531.042462.541719.9177

1200.18700.14001.31250.14621.458787.520827.8729

1500.23500.18801.81250.18992.0024120.145838.2630

1800.24500.19801.91670.25032.1670130.020841.4079

2100.25000.20301.96880.31422.2830136.979243.6239

2400.28000.23302.28130.37992.6611159.666750.8493

2700.31100.26402.60420.45593.0601183.604258.4727

3000.40000.35303.53130.54274.0740244.437577.8463

Kurva hubungan antara t vs jumlah kumulatif APMS yang berpenetrasi ke dalam larutan dapar pfosfat pH 70,05 tiap cm2 pada suhu 37 0,5 C pada membran kulit tikus utuh replikasi 2Hasil Pengukuran Kadar APMS Pada Membran Kulit Tikus Utuh Replikasi 3

waktu (menit)absorbankoreksi absorbankadar (ppm)koreksikadar setelah koreksi (ppm)Jumlah dalam 60 ml (g)Jumlah kumulatif persatuan luas (g/cm2)

00.0470.00000.00000.00000.00000.00000.0000

50.0750.02800.00000.00000.00000.00000.0000

100.0760.02900.00000.00000.00000.00000.0000

150.0870.04000.27080.00000.270816.25005.1752

200.0910.04400.31250.00900.321519.29176.1438

250.0970.05000.37500.01940.394423.66677.5372

300.1150.06800.56250.03190.594435.666711.3588

350.1340.08700.76040.05070.811148.666715.4989

400.1560.10900.98960.07601.065663.937520.3623

450.1770.13001.20830.10901.317479.041725.1725

600.1920.14501.36460.14931.513990.833328.9278

750.2140.16701.59380.19481.7885107.312534.1760

900.2470.20001.93750.24792.1854131.125041.7596

1200.2850.23802.33330.31252.6458158.750050.5573

1500.3590.31203.10420.39033.4944209.666766.7728

1800.3780.33103.30210.49383.7958227.750072.5318

2100.4100.36303.63540.60384.2392254.354281.0045

2400.4350.38803.89580.72504.6208277.250088.2962

2700.4650.41804.20830.85495.0632303.791796.7489

3000.5260.47904.84380.99515.8389350.3333111.5711

Kurva hubungan antara t vs jumlah kumulatif APMS yang berpenetrasi ke dalam larutan dapar pfosfat pH 70,05 tiap cm2 pada suhu 37 0,5 C pada membran kulit tikus utuh replikasi 3

Hasil Pengukuran Kadar APMS Pada Membran Kulit Tikus Utuh Replikasi 4

waktu (menit)absorbankoreksi absorbankadar (ppm)koreksikadar setelah koreksi (ppm)Jumlah dalam 60 ml (g)Jumlah kumulatif persatuan luas (g/cm2)

00.0510.00000.00000.00000.00000.00000.0000

50.0700.01900.00000.00000.00000.00000.0000

100.0810.03000.00000.00000.00000.00000.0000

150.0850.03400.20830.00000.208312.50003.9809

200.0990.04800.35420.00690.361121.66676.9002

250.1070.05600.43750.01880.456327.37508.7182

300.1200.06900.57290.03330.606336.375011.5844

350.1390.08800.77080.05240.823349.395815.7312

400.1500.09900.88540.07810.963557.812518.4116

450.1780.12701.17710.10761.284777.083324.5488

600.1900.13901.30210.14691.449086.937527.6871

750.2110.16001.52080.19031.7111102.666732.6964

900.2500.19901.92710.24102.1681130.083341.4278

1200.3000.24902.44790.30522.7531165.187552.6075

1500.3500.29902.96880.38683.3556201.333364.1189

1800.3760.32503.23960.48583.7253223.520871.1850

2100.4000.34903.48960.59384.0833245.000078.0255

2400.4110.36003.60420.71014.3142258.854282.4376

2700.4700.41904.21880.83025.0490302.937596.4769

3000.4900.43904.42710.97085.3979323.8750103.1449

Kurva hubungan antara t vs jumlah kumulatif APMS yang berpenetrasi ke dalam larutan dapar pfosfat pH 70,05 tiap cm2 pada suhu 37 0,5 C pada membran kulit tikus utuh replikasi 4

Hasil Pengukuran Kadar APMS Pada Membran Kulit Tikus Tanpa Stratum korneum Replikasi 1

waktu (menit)absorbankoreksi absorbankadar (ppm)koreksikadar setelah koreksi (ppm)Jumlah dalam 60 ml (g)Jumlah kumulatif persatuan luas (g/cm2)

00.05300.00000.00000.00000.00000.00000.0000

50.08100.02800.00000.00000.00000.00000.0000

100.09400.04100.00000.00000.00000.00000.0000

150.13600.08300.71880.00000.718843.125013.7341

200.18600.13301.23960.02401.263575.812524.1441

250.20900.15601.47920.06531.544492.666729.5117

300.23100.17801.70830.11461.8229109.375034.8328

350.26300.21002.04170.17152.2132132.791742.2903

400.26600.21302.07290.23962.3125138.750044.1879

450.32100.26802.64580.30872.9545177.270856.4557

600.44400.39103.92710.39694.3240259.437582.6234

750.68400.63106.42710.52786.9549417.2917132.8954

900.79400.74107.57290.74208.3149498.8958158.8840

1201.09901.046010.75000.994411.7444704.6667224.4161

1501.15201.099011.30211.352812.6549759.2917241.8126

1801.16101.108011.39581.729513.1253787.5208250.8028

2101.23601.183012.17712.109414.2865857.1875272.9896

2401.32801.275013.13542.515315.6507939.0417299.0579

2701.62901.576016.27082.953119.22401153.4375367.3368

3001.63901.586016.37503.495519.87051192.2292379.6908

Kurva hubungan antara t vs jumlah kumulatif APMS yang berpenetrasi ke dalam larutan dapar fosfat pH 70,05 tiap cm2 pada suhu 37 0,5 C pada membran kulit tikus tanpa stratum corneum replikasi 1Hasil Pengukuran Kadar APMS Pada Membran Kulit Tikus Tanpa Stratum korneum Replikasi 2waktu (menit)absorbankoreksi absorbankadar (ppm)koreksikadar setelah koreksi (ppm)Jumlah dalam 60 ml (g)Jumlah kumulatif persatuan luas (g/cm2)

00.17600.00000.00000.00000.00000.00000.0000

50.18000.00400.00000.00000.00000.00000.0000

100.29300.11700.00000.00000.00000.00000.0000

150.37000.19401.87500.00001.8750112.500035.8280

200.49800.32203.20830.06253.2708196.250062.5000

250.62300.44704.51040.16944.6799280.791789.4241

300.69900.52305.30210.31985.6219337.3125107.4244

350.96000.78408.02080.49658.5174511.0417162.7521

400.99400.81808.37500.76399.1389548.3333174.6285

451.00000.82408.43751.04319.4806568.8333181.1571

601.11500.93909.63541.324310.9597657.5833209.4214

751.13000.95409.79171.645511.4372686.2292218.5443

901.19701.021010.48961.971912.4615747.6875238.1170

1201.20201.026010.54172.321512.8632771.7917245.7935

1501.26601.090011.20832.672913.8813832.8750265.2468

1801.27101.095011.26043.046514.3069858.4167273.3811

2101.36301.187012.21883.421915.6406938.4375298.8654

2401.38901.213012.48963.829216.3188979.1250311.8232

2701.49301.317013.57294.245517.81841069.1042340.4790

3001.68501.509015.57294.697920.27081216.2500387.3408

Kurva hubungan antara t vs jumlah kumulatif APMS yang berpenetrasi ke dalam larutan dapar fosfat pH 70,05 tiap cm2 pada suhu 37 0,5 C pada membran kulit tikus tanpa stratum corneum replikasi 2Hasil Pengukuran Kadar APMS Pada Membran Kulit Tikus Tanpa Stratum korneum Replikasi 3

waktu (menit)absorbankoreksi absorbankadar (ppm)koreksikadar setelah koreksi (ppm)Jumlah dalam 60 ml (g)Jumlah kumulatif persatuan luas (g/cm2)

00.0510.00000.00000.00000.00000.00000.0000

50.0840.03300.00000.00000.00000.00000.0000

100.0850.03400.00000.00000.00000.00000.0000

150.0960.04500.32290.00000.322919.37506.1704

200.1180.06700.55210.01080.562833.770810.7550

250.2150.16401.56250.02921.591795.500030.4140

300.3450.29402.91670.08132.9979179.875057.2850

350.3570.30603.04170.17853.2201193.208361.5313

400.3630.31203.10420.27993.3840203.041764.6630

450.4540.40304.05210.38334.4354266.125084.7532

600.4930.44204.45830.51844.9767298.604295.0969

750.5530.50205.08330.66705.7503345.0208109.8792

900.7130.66206.75000.83657.5865455.1875144.9642

1201.0791.028010.56251.061511.6240697.4375222.1139

1501.1881.137011.69791.413513.1115786.6875250.5374

1801.2191.168012.02081.803513.8243829.4583264.1587

2101.2471.196012.31252.204214.5167871.0000277.3885

2401.3341.283013.21882.614615.8333950.0000302.5478

2701.5001.449014.94793.055218.00311080.1875344.0088

3001.6711.620016.72923.553520.28261216.9583387.5663

Kurva hubungan antara t vs jumlah kumulatif APMS yang berpenetrasi ke dalam larutan dapar fosfat pH 70,05 tiap cm2 pada suhu 37 0,5 C pada membran kulit tikus tanpa stratum corneum replikasi 3Hasil Pengukuran Kadar APMS Pada Membran Kulit Tikus Tanpa Stratum korneum Replikasi 4

waktu (menit)absorbankoreksi absorbankadar (ppm)koreksikadar setelah koreksi (ppm)Jumlah dalam 60 ml (g)Jumlah kumulatif persatuan luas (g/cm2)

00.0870.00000.00000.00000.00000.00000.0000

50.1800.09300.00000.00000.00000.00000.0000

100.3230.23600.00000.00000.00000.00000.0000

150.3500.26302.59380.00002.5938155.625049.5621

200.4500.36303.63540.08653.7219223.312571.1186

250.5870.50005.06250.20765.2701316.2083100.7033

300.6740.58705.96880.37646.3451380.7083121.2447

350.8900.80308.21880.57538.7941527.6458168.0401

400.9780.89109.13540.84939.9847599.0833190.7909

450.9450.85808.79171.15389.9455596.7292190.0411

601.0530.96609.91671.446911.3635681.8125217.1377

751.1111.024010.52081.777412.2983737.8958234.9987

901.1871.100011.31252.128113.4406806.4375256.8272

1201.2121.125011.57292.505214.0781844.6875269.0088

1501.2541.167012.01042.891014.9014894.0833284.7399

1801.2611.174012.08333.291315.3747922.4792293.7832

2101.3531.266013.04173.694116.73581004.1458319.7917

2401.4561.369014.11464.128818.24341094.6042348.6001

2701.6531.566016.16674.599320.76601245.9583396.8020

3001.7601.673017.28135.138222.41941345.1667428.3970

Kurva hubungan antara t vs jumlah kumulatif APMS yang berpenetrasi ke dalam larutan dapar fosfat pH 70,05 tiap cm2 pada suhu 37 0,5 C pada membran kulit tikus tanpa stratum corneum replikasi 4