percobaan uji kuantitatif protein

7/24/2019 Percobaan Uji Kuantitatif Protein

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LAPORAN TETAP PRAKTIKUM BIOKIMIA II

UJI KUANTITATIF PROTEIN

Nama : Lusiana Setiawati

NIM : 061011813008

Ke!"m#"$ : 1 % Satu&

An''"ta :1( )ia* Pe+mata Sa+i

( Nu+u! Mawa,,a*

3( Met- T++ian-

.( Reni Ma+/e!a

( Reni O$taia 2ana+ Na*"+

6( 4i!,an Ti$t" R

)"sen Pem5im5in' : )esi S(P,( M(T(

)ia* Ka+ti$a Sa+i S(P,( M(Si(

PROGRAM STUDI PENDIDIKAN KIMIA

FAKULTAS KEGURUAN DAN ILMU PENDIDIKAN

UNIVERSITAS SRIWIJAYA

2016


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LAPORAN TETAP PRAKTIKUM BIOKIMIA II

I. PERCOBAAN KE : 4 (E!"#$

II. TANGGAL PRAKTIKUM : 12 F%&'"') 2016

III. JUDUL PRAKTIKUM : U*) K"+#)#"#), P'-#%)+

IV. TUJUAN PRAKTIKUM :

Menentu$an $a,a+ #+"tein -an' te+,a#at ,a!am sam#e! ,en'an met",e tit+asi 7"+m"!(

V. DASAR TEORI

P+"tein ,itemu$an ,i ,a!am semua se! ,an semua 5a'ian se! -an' amat

5e+a+iasi -aitu te+,a#at +atusan enis -an' 5e+5e,a ,a#at ,itemu$an ,a!am satu se!(

P+"tein 5e+#e+an #entin' ,a!am st+u$tu+ ,an 7un'si semua se! ma$*!u$ *i,u# ,an

i+us( Pe+anan #entin' #+"tein anta+a !ain untu$ mem5entu$ a+in'an "t"t /at #en'atu+

se+ta se5a'ai a,an'an ma$anan ,an ene+'i ,a!am tu5u*( P+"tein me+u#a$an sa!a*satu ,a+i 5i"m"!e$u! +a$sasa se!ain #"!isa$a+i,a !i#i, ,an #"!inu$!e"ti,a -an'

me+u#a$an #en-usun utama ma$*!u$ *i,u# -an' u'a me+u#a$an #"!ime+ ,a+i

m"n"me+9m"n"me+ asam amin" -an' ,i*u5un'$an satu sama !ain ,en'an i$atan

#e#ti,a(

P+"tein 5e+asa! ,a+i 5a*asa unani #+"t"s -an' 5e+a+ti ;-an' #a!in' utama= "$si'en 3= nit+"'en 6= ,an $a,an' $a!a

su!7u+ 093= se+ta 7"s7"+ 093=( P+"tein ,a#at ti,a$ sta5i! te+*a,a# 5e5e+a#a 7a$t"+ -aitu

#? +a,iasi su*u me,ium #e!a+ut "+'ani$ ,an ,ete+'en( P+"tein ,a#at ,i#e+"!e* ,a+i

ma$anan -an' 5e+asa! ,a+i *ewan atau tum5u*an( P+"tein -an' 5e+asa! ,a+i *ewan

,ise5ut #+"tein *ewani se,an'$an -an' 5e+asa! ,a+i tum5u*an ,ise5ut #+"tein na5ati(

2e5e+a#a ma$anan sum5e+ #+"tein a,a!a* ,a'in' te!u+ susu i$an 5e+as $aan'

$e,e!ai 'an,um a'un' ,an 5ua*95ua*an( Tum5u*an mem5entu$ #+"tein ,a+i @O

?O ,an sen-awa nit+"'en( ?ewan -an' ma$an tum5u*an men'u5a* #+"tein na5ati

mena,i #+"tein *ewani( )isam#in' ,i'una$an untu$ #em5entu$an se!9se! tu5u*

#+"tein u'a ,a#at ,i'una$an se5a'ai sum5e+ ene+'i a#a5i!a tu5u* $e$u+an'an

$a+5"*i,+at ,an !ema$( San'at !ua+ 5iasa #u!a 5a*wa semua #+"tein ,i ,a!am semua

ma$*!u$ tan#a meman,an' 7un'si ,an a$tiitas 5i"!"'in-a ,i5an'un "!e* susunan

,asa+ -an' sama -aitu 0 asam amin" 5a$u -an' m"!e$u!n-a sen,i+i ti,a$

mem#un-ai a$tiitas 5i"!"'i( Sea+a u$u# se,e+*ana #+"tein 5e+5e,a satu sama !ain

$a+ena masin'9masin' mem#un-ai deretunit asam amin" sen,i+i9sen,i+i( Asam amin"


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me+u#a$an a5a, st+u$tu+ #+"tein $a+ena m"!e$u!9m"!e$u! ini ,a#at ,isusun ,a!am

um!a* ,e+et -an' *am#i+ ti,a$ te+5atas untu$ mem5uat 5e+5a'ai #+"tein ,a!am

um!a* -an' *am#i+ ti,a$ te+5atas #u!a %Le*nin'e+ 18&

)a!am $e*i,u#an #+"tein meme'an' #e+anan -an' #entin' #u!a( P+"ses $imia

,a!am tu5u* ,a#at 5e+!an'sun' ,en'an 5ai$ $a+ena a,an-a en/im suatu #+"tein -an'

5e+7un'si se5a'ai 5i"$ata!is( )i sam#in' itu *em"'!"5in ,a!am 5uti+95uti+ ,a+a*

me+a* atau e+it+"sit -an' 5e+7un'si se5a'ai #en'an'$ut "$si'en ,a+i #a+u9#a+u $e

se!u+u* 5a'ian tu5u* a,a!a* sa!a* satu enis #+"tein( )emi$ian #u!a /at9/at -an'

5e+#e+an untu$ me!awan 5a$te+i #en-a$it atau -an' ,ise5ut anti'en u'a suatu #+"tein

%P"e,ia,i 00&(

VI. ALAT DAN BAAN

A/"#1( E+!enme-e+

( Stati7

3( 2u+et

.( Pi#et tetes

( Be!as u$u+

6( M"+ta!

>( @awan #"+se!en

8( Ke+tas sa+in'

B""+

1( La+utan sam#e! % "$!at tem#e ,an nasi&

( NaO? 01 M

3( ACua,est

.( K9O$sa!at enu*

( F"+ma!,e*i, .0=

VII. PROSEDUR PERCOBAAN

G"&"' K%#%'"+"+

?a!us$an Sam#e!


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Tim5an' Sam#e! se5an-a$ '+am

La+ut$an sam#e! ,en'an 100 m! aCua,es

Sa+in' !a+utan sam#e!

Men'am5i! 10 mL !a+utan sam#e! $e ,a!am

e+!enme-e+ 100 mL $emu,ian menam5a*$an 0 mL

aCua,est ,an 0. mL K9"$sa!at enu* se+ta 1 mL

in,i$at"+ PP ,an men,iam$ann-a se!ama menit(

Mentit+asi sam#e ,en'an !a+utan NaO? 01 N

sam#ai !a+utan 5e+wa+na #in$(

Kemu,ian menam5a*a$an mL !a+utan 7"+ma!,e*i,

.0 = !a!u me!anut$an menit+asi sam#ai !a+utan

5e+wa+na #in$(

Menatat "!ume NaO? -an' te+#a$ai(


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VIII. ASIL PENGAMATAN

L"'#"+ B/"+-

T)#'"3

)

V-/

N"O

P%+"&""

+

F-'"/%)

V-/

N"O

G"&"'

1 01 mL 2enin' 01 mL

2 01 mL 2enin' 0 mL

L"'#"+ S"!%/

S"!%/ V-/ N"O G"&"'

T%!%

1 0> mL

08 mL

C-/"#

1 0> mL

08 mL

N"3) 1 03 mL

03 mL


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I5. PERSAMAAN REAKSI

5. PERITUNGAN

%Protein=Volume NaOH N NaOH 14,008

Berat sampel10mL 100

)i$eta*ui: 2e+at sam#e! : '+am

M NaO? : 01 M

Volume NaOH=Volume Titrasi I+Volume Titrasi2

2


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Tempe=0,7+0,8

2=0,75mL

Coklat=0,7+0,8

2

=0,75mL

Nasi=0,3+03,

2=0,3mL

)itan-a: = P+"teinD

Pen-e!esaian:

%ProteinTempe=0,750,114,008

510mL 100=2,1

%ProteinCoklat=0,750,114,008

510mL 100=2,1

%Protein Nasi=0,30,114,008

510mL 100=0,8

5I. PEMBAASAN

Pe+"5aan $a!i ini -aitu tentan' #+"tein( P+"tein me+u#a$an suatu sen-awa#"!ime+ -an' te+5entu$ ,a+i m"n"me+9m"n"me+ asam amin" -an' ,i*u5un'$an "!e*

i$atan #e#ti,a anta+a asam amin" satu ,en'an asam amin" !ainn-a( P+"tein 5e+7un'si

mem5entu$ a+in'an atau 5a'ian tu5u* !ain untu$ #e+tum5u*an se5a'ai en/im

%me+u#a$an $ata!isat"+& t+ans#"+t m"!e$u! ,i ,a!am ,a+a* ,an se! se5a'ai *"+m"ne

"nt"*n-a *"+m"ne insu!in se5a'ai #em5entu$ anti5",- m"!e$u! -an' mem5antu

$"nt+a$si "t"t $eseim5an'an ai+an t+ansmisi sa+a7(

)a!am #e+"5aan ini !a+utan sam#e! ,i5uat ,en'an me!a+utan '+am sam#e!

$e,a!am 100 mL aCua,es a,a #e+!a$uan -an' ,i5e+i$an #a,a sam#e! !a+utan tem#e

!a+utan "$!at ,an !a+utan nasi( Se#e+ti #enam5a*an ai+ #a,a $eti'a sam#e! ini

5e+tuuan untu$ men'*i,+"!isis #+"tein -an' te+,a#at #a,a sam#e! tem#e "$!at ,an

nasi mena,i asam9asam amin"( Penam5a*an ,inat+ium "$sa!at enu* 5e+tuuan untu$

mem5!"$a,e 'u'us amina %9N?& #a,a asam amin" se*in''a *an-a te+,a#at 'u'us

$a+5"$si! %9@OO?& #a,a uun' +antai -an' a$an 5e+ea$si ,en'an NaO? sam#ai !autan

te+se5ut 5e+u5a* mena,i 5e+wa+na me+a* mu,a( Penam5a*an In,i$at"+ PP 5e+tuuan

untu$ se5a'ai 5atas #enan,a 5e+a$*i+n-a tit+asi( Pe+!a$uan se!anutn-a semua !a+utan


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,itit+asi ,en'an !a+utan NaO? 01 N -an' 5e+tuuan untu$ menet+a!$an 'u'us

$a+5"$si! -an' te+,a#at #a,a asam amin"( Saat mentit+asi $eti'a sam#e! !a+utan ,a+i

!a+utan tem#e "$!at ,an nasi wa+na !a+utan 5e+u5a* mena,i #in$( Pa,a #e+!a$uan

se!anutn-a #enam5a*an ,en'an 7"+ma!,e*i, -an' 5e+tuuan untu$ men'uat$an si7at

asam ,a+i asam amin" *a! ini ,itan,ai ,en'an *i!an'n-a wa+na #in$ #a,a !a+utan

se!anutn-a !a+utan ,itit+asi $em5a!i sam#ai !a+utan 5e+u5a* mena,i #in$ $em5a!i(

Pe+u5a*an wa+na !a+utan mena,i #in$ ,ise5a5$an $a+ena si7at ,a+i in,i$at"+ ## -an'

a$an 5e+wa+na #in$ #a,a !a+utan 5asa %se#e+ti NaO?&(

Pa,a 5e+"5aan men''una$an !a+utan 5!an$" ,i'una$an aCua,es -an'

,itam5a*$an ,en'an )inat+ium "$sa!at enu* ,an in,i$at"+ ## wa+na !a+utan !an'sun'

5e+u5a* #in$ ini menan,a$an 5a*wa !a+utan 5!an$" ini ti,a$ men'an,un' #+"tein(

A,a#un tuuan ,a+i !a+utan 5!an$" ini -aitu untu$ men'eta*ui um!a* m! NaO? -an'

5e+ea$si ,en'an /at9/at $imia -an' ,i'una$an ,a!am ana!isis -aitu )inat+ium "$sa!at

enu* 7"+ma!,e*i, ,an ai+(

)a+i *asi! tit+asi ,i#e+"!e* "!ume NaO? -an' ,i'una$an untu$ !a+utan

tem#e "$!at ,an nasi a,a!a* 0> mL 0> mL ,an 03 mL( Pe+5e,aan "!ume

te+se5ut ,ise5a5$an $a+ena $a,a+ #+"tein ,a+i tia#9ti# sam#e! 5e+5e,a( 2e+,asa+$an

#e+*itun'an ma$a ,a#at ,i$eta*ui $a,a+ #+"tein ,a+i sam#e! -an' ,i'una$an -aitu

untu$ !a+utan sam#e! tem#e 1= "$!at 1= ,an nasi 08=(

5II. KESIMPULAN

1( P+insi# tit+asi 7"+m"! a,a!a* met",e ana!isis a+a #enentuan #+"tein ,en'an a+a

tit+asi 7"+m"! untu$ men'*i,+"!isis #+"tein ,a!am sam#e!(

( Sema$in 5an-a$ !a+utan NaO? -an' ,i'una$an ma$a sema$in tin''i $a,a+

#+"teinn-a 5e'itu#un se5a!i$n-a(

3( Sam#e! -an' memi!i$i $a,a+ #+"tein te+tin''i a,a!a* tem#e ,an "$!at se,an'$an

-an' memi!i$i $a,a+ #+"tein +en,a* a,a!a* nasi(

.( Ka,a+ #+"tein -an' ,i#e+"!e* me!a!ui #e+"5aan untu$ tem#e a,a!a* 1= "$!at

1= ,an nasi 08=(


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5III. DAFTAR PUSTAKA

Fau/ia* N( %01. Ju!i&(Analisa Protein

( )i#eti$ Fe5+ua+i 0 016 ,a+i *tt#:nu+u!9797#$11(we5(unai+(a(i,a+ti$e!,etai!910>>69Umum9Ana!isa

=0P+"tein(*tm!(

Le*nin'e+ A( L( %18&(Dasar - Dasar Biokimia.Ja$a+ta: E+!an''a(

P"e,ia,i A( %1.&(Dasar - Dasar Biokimia .Ja$a+ta: Unie+sitas In,"nesia(

percobaan uji kuantitatif protein

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