kel 2_kemampuan memproduksi imunoglobulin

8/16/2019 Kel 2_kemampuan Memproduksi Imunoglobulin

1/14

KEMAMPUANMEMPRODUKSI

IMUNOGLOBULINKELOMPOK 2 :

SHINTA ANGGRAINI (I21112060)

RUTH HARYATI BUTAR-BUTAR(I21112070)NUR AFIFAH (I21112077)

INNE NOVIANTI (I21112087)


2/14

DEFINISI

Immunoglobulin !"!#!$ %&'!! *+,&.' !'/"./'!!' ' !!' !' *&'!.(3.+%4 5!&+.4 +!' 5!&+. "##)4 &+"!*!

"!#! %&+ !! !.+!' 5$ !'/ 5.!%!'!

".%&5 !'.5,".

K&!*!' *&'"&+.! &5&'.',/#,5#.' "!*! ".*&+.%! "&'/!'

.'.%!%. !.4 .%!#'! !'./&' 5!&+. "!'+&%*,' !'.5,". ".+ "&'/!' !5'/ &%

*+&%.*.!%.


3/14

9URNAL :

P+&*!+!%. I',/#,5#.' G K.'. %&5!/!.A'./&' P&'/.'"%. A'.5,". S*&%. T&+$!"!*V.+% Avian Infuenza H;N1 S+!.' L&/,


4/14

PRINSIP PEMERIKSAAN

P&&+.%!!!' %&!+! !#.!. ".#!!'"&'/!' &,"& AGPT(A/!+ G P+&.*.!.,'

T&%) ".!'! $!%.# *,%.. ".'


5/14

TU9UAN PEMERIKSAAN

T


6/14

LANGKAH-LANGKAH PEMERIKSAAN

1


7/14

R&."&'.!%. V!%.' A1 H;N1 I'!. S+!.' L&/,

RNA "!+. 3!%.' AI H;N1 .'!. %+!.' L&/,".&%+!%.

D.."&'.!%. %5 .*& 3.+% AI-'! 5&+"!%!+!' /&'$&!/#.'.' (HA) "!' '&+!.'."!%& (NA)

I%,#!%. RNA ".#!!' "&'/!' &'//'!!' &,"& TRI@OL

T!$!* !$.+ !"!#!$ #!+!' RNA "..'5!%.!'"!#! *&'!'/!% !.+ 60 %!! 10 &'.

L!+!' RNA ".%.*!' *!"! -20 %!*!. ".#!!'

RT-PR


8/14

P+,"%. A'.5,". P,#.#,'!# AI H;N1

M&''.!' %! ",%.% 3!%.' AI H;N1 .'!. %+!.' L&/, (04;#) %&!+! %5!' *!"! 10 &,+ .'&! *./%

D.#!!' *&''.!' ".#!'/ %&5!'! "! !#. "&'/!' .'&+3!#

C .'//

A'.5,". "."&&%. "&'/!'


9/14

R&."&'.!%. S&+ A'. A1 H;N1 (A51)

R&."&'.!%. %&+ !+ ".#!!' "&'/!' HIT "!'AGPT

S&5 ".#!!' HIT4 %&+ !+ "..'!.3!%.

&+#&5.$ "!$# "!#! *&'!'/!% !.+ ;6 %!! C0&'.

D.!5%,+*%. "&'/!' % "!+!$ &+!$ !! *!"!&*&+!+ +!'/ %!! C0 &'.

D.%&'+./!%. "&'/!' &&*!!' 1000 / %!! ; &'."!' ".!5.# %*&+'!!''!

S!'


10/14

P&,,'/!' I',/#,5#.' GA1 H;N1 "!' P+.!%. F+!/&' F (!5)2

D.!5.# %&+ !+ ' &*&+,#&$ +!/&' "!+..',/#,5#.'

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%&!$ . ".$&'.!' "&'/!' T+.% B&+

D.%&'+./!%. "&'/!' &&*!!' 10000 / %!! C0 &'.

L!+!' &".!' disalting "&'/!' HiTrap Desalting "!'

".$.'/ "&'/!' &'//'!!' %*&+,,,&&+ U#+! V.,#&

P . I # 5 #. G A . AI


11/14

P&+'.!' I',/#,5#.' G A'. AIH;N1(A51) "!' A'.5,". A'.-.".,.*&

(A52)P&+'.!' &'//'!!' ,'!/& !'.5," *+.!.,' . %*.',#' .$ P+,%&* A &".! (M.##.*,+&)

P+,%&* A ".!%!' & "!#! %*.' ,#, ".. 5.'".'/ 5&+".%&'+.% 1; &'.

S&+ ".#&+ "!' %!* ".#!+!' "!' ".!%!' & ,#, %*.'"!' ".%&'+./!%.

K,#, %*.' "!' ".%&'+.% %!! ; &'.4 *&'.!' ".#!!' "!!#.

I/G ".%. "!#! !5'/ %&'+.% %!! ; &'. I/G !"!#!$

#!+!' !'/ ".5!!$4

I/G ". "&%!#.'/ "!' ".%&'+.%4 I/G !"!#!$ #!+!' !'/ ".!!%

R&."&'.!%. I/G AI H;N1 "&'/!'


12/14

P+,"%. I',/#,5#.' G K.'.

A'.-I".,.*& (A52)

I',/#,5#.' G A52 ".%.!*!' "&'/!' !+! &''.!'.'. "&'/!' A5 F(A5)2

K.'. "..'.%!%. "&'/!' ;00 / A51 H;N1 "!#! FA %&!+!.'+!%#!+

D.#!!' .'.%!%. #!'/!' %! .'// 5&+.'! "!'%&+ ".,#&%.

D.."&'.!%. &5&+!"!!' A52 H;N1 %+!.' L&/, "&'/!' AGPT

D.&'!' 5&+! ,#&# "&'/!' &,"& SDS-PAGE "!','%&'+!%.'! ".$.'/ "&'/!' %*&+,,,&&+ #+! 3.,#&


13/14


14/14

MANIFESTASI KLINIS

kel 2_kemampuan memproduksi imunoglobulin

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