i

LAPORAN

PENELITIAN STRATEGIS NASIONAL

KERAGAMAN GENETIK GENERASI 1 SELFING JAGUNG

LOKAL SULAWESI SELATAN BERDASARKAN

MARKA SIMPLE SEQUENCE REPEAT (SSR)

UNTUK PEMBENTUKAN GALUR

JAGUNG PROVITAMIN A

TIM PENELITI

DR. JUHRIAH, M.Si. NIDN 0031126308 (KETUA)

DR. A. MASNIAWATI, S.Si, M.Si. NIDN 0013027003 (ANGGOTA)

PROF. DR. IR. MIR ALAM, M.Si. NIDN 0031126306 (ANGGOTA)

UNIVERSITAS HASANUDDIN MAKASSAR

NOVEMBER 2014

Kode/ NamaRumpunIlmu: 113 (Biologi&BioteknologiUmum)

Tema : CLIMATE CHANGE AND BIODIVERSITY

iii

GENETIC DIVERSITY 1ST

GENERATION SELFING LOCAL CORN

OF SOUTH SULAWESI BASED ON MOLECULAR MARKERS

SIMPLE SEQUENCE REPEAT (SSR) FOR DEVELOPING

A STRAIN OF PROVITAMIN A OF CORN

Juhriah1, A. Masniawati

1, Mir Alam

2

1. Biologi Departement of FMIPA, Hasanuddin University, Makassar

2. Agriculture Faculty of Indonesia Timur University, Makassar

juhriah@gmail.com

ABSTRACT

Vitamin A deficiency (VAD) is a major cause of premature death in developing nations..

Among the major cereals, yellow endosperm maize is the only crop having appreciable

amount of carotenoids with a wide range of genetic variability. Several carotinoids have

provitamin-A activity due to the presence of a vitamin structure that is a part of the overall

carotenoid compound. South Sulawesi germplasm lokal corn containing high

carotenoids and phytoene synthase gene (PSY 1) as found in the original corn CYMMYT, it

means that South Sulawesi local corn germplasm has the potential to be developed into a

genetic source for the developing of varieties of corn provit A. The research aims to analysis

genetic diversity of 1st generation of selfing local corn South Sulawesi based on Simple

Sequence Repeats markers was carried out. There are 52th

samples were analysis with 10th

Simple Sequence Repeats primers, at Molecular Laboratory the Indonesian Cereals Research

Institute (ICERI) in Maros South Sulawesi. Procedure for DNA extraction, Polymerase Chain

Reaction and Electrophoresis, followed the instruction of AMBIONET. Analysis of data

using NTSYST program. The coefficient of similarity with the Simple Matching Coefficient,

and clustering by Unweighted Pair Group Aritmathic Analysis. The smallest genetic

similarity coefficient (0.48) between L3 with F1, F3 and F4 (Srikandi kuning 1 individual 3

with 3 individual Kandora local corn from Tana Toraja at South Sulawesi) and J3. while the

highest similarity value (1.0) between J3 with F1, F3 and F4 (Kui caroteniod Syn individual 3

corn from CIMMYT with 3 individual Kandora local corn from Tana Toraja). Dendrogram

showed that at 0.72 the degree of similarity, the 52 individuals that form seven groups,

there were two groups containing only one individual.

Keywords: genetic diversity, corn, Sulawesi Selatan, CIMMYT, Simple Sequence Repeats