iv

DAYA HAMBAT INFUSA BATANG DAN DAUN SAMBILOTO

(Andrographis paniculata)TERHADAP PERTUMBUHAN

Candida albicans

Ekya Salma Dimar Nozelia1, Sri Sinto Dewi2, Wildiani Wilson2

1. Program Studi D IV Analis Kesehatan Fakultas Ilmu Keperawatan dan Kesehatan Universitas

Muhammadiyah Semarang, 2. Laboratorium Mikrobiologi Fakultas Ilmu Keperawatan dan Kesehatan Universitas

Muhammadiyah Semarang.

ABSTRAK

Penelitian bertujuan mengetahui daya hambat infusa batang dan daun sambiloto terhadap

pertumbuhan C. albicans penyebab kandidiasis berdasarkan konsentrasi 10%, 20%, 30%, 40%,

dan 50%, menganalisis perbedaan daya hambat infusa batang dan daun sambiloto. Jamur uji C.

albicans diperoleh dari stok isolat Laboratorium Mikrobiologi Universitas Muhammadiyah

Semarang, sedangkan sampel batang dan daun sambiloto diperoleh dari Desa Penggaron Kidul, Kecamatan Pedurungan, Kota Semarang. Penelitian ini meliputi kultivasi jamur C. albicans,

pembuatan sediaan infusa batang dan daun sambiloto, pengujian aktifitas antifungi dilakukan

menggunakan metode difusi sumuran/perforasi dengan diameter 6 mm. Hasil menunjukan rata-

rata diameter zona hambat infusa batang konsentrasi 30% 15 mm sedangkan infusa daun 16,33

mm, konsentrasi 40% infusa batang 17,33 mm sedangkan infusa daun 18mm, dan konsentrasi 50%

infusa batang 19,66 mm sedangkan infusa daun 20 mm. Hasil uji Wilxocon Signed Rank-Test

menunjukkan adanya perbedaan daya hambat antara infusa batang dan daun sambiloto terhadap

petumbuhan C. albicans. Hasil uji Regresi Linear Sederhana menunjukan infusa daun sambiloto

memiliki daya hambat lebih baik daripada infusa batang sambiloto.

Kata kunci : Candida albicans, Daya Hambat, Infusa, Sambiloto

repository.unimus.ac.id

v

INHIBITORY OF INFUSA STEAM AND SAMBILOTO LEAVES

(Andrographis paniculata) ON GROWTH OF Candida albicans

Ekya Salma Dimar Nozelia1, Sri Sinto Dewi2, Wildiani Wilson2

1. Medical Laboratory D IV Study Programme of Health dan Nursing Faculty Muhammadiyah University of Semarang

2. Microbiology Laboratory at Health and Nursing Faculty Muhammadiyah University of

Semarang

ABSTRACT

The purpose of this research is to know the inhibition of infusa steam and sambiloto leaves on

growth on C. albicans causes of candidiasis based on concentration 10%, 20%, 30%, 40%, and

50%, analyzing the difference of inhibition between infusa steam and sambiloto leaves. C.

albicans test fungi were obtained from Microboilogy Laboratory isolate stock of Muhammadiyah

University of Semarang, while steam and sambiloto leaves obtained from Penggaron Kidul

Village, Pedurungan, Semarang. This research included C. albicans cultivation, preparation of infusa steam and sambiloto leaves, antifungal activity testing was performed using diffusion of

wells/perforation method with diameter 6 mm. The result showed average diameter of inhibition

zone of infusa steam concentration 30% 15 mm while infusa leaf 16,33 mm, concentration 40%

infusa steam 17,33 mm while infusa leaf 18 mm, and concentration 50% of infusa steam 19,66

mm and infusa leaf 20 mm. Result of Wilxocon Signed Rank Test showed there is the differences

of inhibition between infusa steam and sambiloto leaves on growth of C. albicans. Result of

Simple Regretion Linear Test showed infusa leaf of sambiloto have a inhibition better than

sambiloto steam.

Keywords : Candida albicans, Inhibition, Infusa, Sambiloto

repository.unimus.ac.id