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LAMPIRAN KINETIKA REAKSI ENZIMATIS

Tabel 1. Kurva StandarKonsentrasi glukosa

(mg/ml)Absorbansi 550nm

0 0.4010.05 0.670.1 1.030.15 1.050.2 1.3390.25 1.6190.3 1.6750.35 1.967

0 0.05 0.1 0.15 0.2 0.25 0.3 0.35 0.40

0.5

1

1.5

2

2.5

f(x) = 4.29595238095238 x + 0.467083333333333R² = 0.980345424742644

Grafik Kurva Standar

Kurva StandarLinear (Kurva Standar)

Konsentrasi Glukosa (mg/ml)

Abs

orba

nsi

Tabel 2. Absorbansi beberapa konsentrasi amilum yang dihidrolisis amilase

Waktu (menit)

Absorbansi 550 nmAmilum

0.1%Amilum

0.2%Amilum

0.3%Amilum

0.4%Amilum

0.5%0 0.34 0.668 1.14 0.766 0.8755 0.671 0.904 1.1426 1.113 1.43210 0.79 1.332 1.69 1.377 1.73215 1.135 1.374 1.79 1.663 2.5720 1.636 1.488 2.303 1.83 2.33625 1.76 1.734 2.591 2.92 2.3830 1.607 1.879 2.533 2.866 2.98

0 5 10 15 20 25 30 350

0.5

1

1.5

2

2.5

3

3.5

f(x) = 0.0629642857142857 x + 1.09910714285714R² = 0.870122450590258f(x) = 0.07405 x + 0.679964285714286R² = 0.929418287884712f(x) = 0.05492 x + 1.06042857142857R² = 0.939924071054232

f(x) = 0.0389214285714286 x + 0.756035714285714R² = 0.954845756185485f(x) = 0.04875 x + 0.402892857142857R² = 0.908694781438623

Grafik Absorbansi terhadap Waktu 0.10%

Linear (0.10%)

0.20%

Linear (0.20%)

0.30%

Linear (0.30%)

0.40%

Linear (0.40%)

0.50%

Linear (0.50%)

Waktu (menit)

Abs

orba

nsi

Tabel 3. Konsentrasi Glukosa

Waktu (menit)

Konsentrasi glukosaAmilum

0.1%Amilum

0.2%Amilum

0.3%Amilum

0.4%Amilum

0.5%0 -0.03 0.05 0.16 0.07 0.095 0.05 0.10 0.16 0.15 0.2210 0.08 0.20 0.28 0.21 0.2915 0.16 0.21 0.31 0.28 0.4920 0.27 0.24 0.43 0.32 0.4425 0.30 0.29 0.49 0.57 0.4530 0.27 0.33 0.48 0.56 0.58

0 5 10 15 20 25 30 35

-0.10

0.00

0.10

0.20

0.30

0.40

0.50

0.60

0.70

f(x) = 0.0146564910880553 x + 0.147115256717212R² = 0.870122450590258f(x) = 0.0172369646182495 x + 0.0495494147379622R² = 0.929418287884712f(x) = 0.0127839851024209 x + 0.138111864857675R² = 0.939924071054232

f(x) = 0.0090599228518223 x + 0.0672569167331736R² = 0.954845756185485f(x) = 0.0113477653631285 x − 0.014945796754456R² = 0.908694781438623

Grafik Konsentrasi Glukosa terhadap Waktu

0.10%Linear (0.10%)0.20%Linear (0.20%)0.30%Linear (0.30%)0.40%

Waktu (menit)

Kons

entr

asi G

luko

sa

Tabel 4. SlopeS (Konsentrasi Amilum) % 1/S V 1/V

0.1 10.00 0.0113 88.500.2 5.00 0.0091 109.890.3 3.33 0.0128 78.130.4 2.50 0.0172 58.140.5 2.00 0.0147 68.03

1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.0011.000.00

20.00

40.00

60.00

80.00

100.00

120.00

f(x) = 3.26291878728677 x + 65.6348237139418R² = 0.28202175473797

Hubungan 1/V terhadap 1/S

Hubungan 1/V terhadap 1/S

Linear (Hubungan 1/V terhadap 1/S)

1/S

1/V

Hasil NilaiVmax 0.015235774Km 0.049712806