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LAMPIRAN KINETIKA REAKSI ENZIMATIS
Tabel 1. Kurva StandarKonsentrasi glukosa
(mg/ml)Absorbansi 550nm
0 0.4010.05 0.670.1 1.030.15 1.050.2 1.3390.25 1.6190.3 1.6750.35 1.967
0 0.05 0.1 0.15 0.2 0.25 0.3 0.35 0.40
0.5
1
1.5
2
2.5
f(x) = 4.29595238095238 x + 0.467083333333333R² = 0.980345424742644
Grafik Kurva Standar
Kurva StandarLinear (Kurva Standar)
Konsentrasi Glukosa (mg/ml)
Abs
orba
nsi
Tabel 2. Absorbansi beberapa konsentrasi amilum yang dihidrolisis amilase
Waktu (menit)
Absorbansi 550 nmAmilum
0.1%Amilum
0.2%Amilum
0.3%Amilum
0.4%Amilum
0.5%0 0.34 0.668 1.14 0.766 0.8755 0.671 0.904 1.1426 1.113 1.43210 0.79 1.332 1.69 1.377 1.73215 1.135 1.374 1.79 1.663 2.5720 1.636 1.488 2.303 1.83 2.33625 1.76 1.734 2.591 2.92 2.3830 1.607 1.879 2.533 2.866 2.98
0 5 10 15 20 25 30 350
0.5
1
1.5
2
2.5
3
3.5
f(x) = 0.0629642857142857 x + 1.09910714285714R² = 0.870122450590258f(x) = 0.07405 x + 0.679964285714286R² = 0.929418287884712f(x) = 0.05492 x + 1.06042857142857R² = 0.939924071054232
f(x) = 0.0389214285714286 x + 0.756035714285714R² = 0.954845756185485f(x) = 0.04875 x + 0.402892857142857R² = 0.908694781438623
Grafik Absorbansi terhadap Waktu 0.10%
Linear (0.10%)
0.20%
Linear (0.20%)
0.30%
Linear (0.30%)
0.40%
Linear (0.40%)
0.50%
Linear (0.50%)
Waktu (menit)
Abs
orba
nsi
Tabel 3. Konsentrasi Glukosa
Waktu (menit)
Konsentrasi glukosaAmilum
0.1%Amilum
0.2%Amilum
0.3%Amilum
0.4%Amilum
0.5%0 -0.03 0.05 0.16 0.07 0.095 0.05 0.10 0.16 0.15 0.2210 0.08 0.20 0.28 0.21 0.2915 0.16 0.21 0.31 0.28 0.4920 0.27 0.24 0.43 0.32 0.4425 0.30 0.29 0.49 0.57 0.4530 0.27 0.33 0.48 0.56 0.58
0 5 10 15 20 25 30 35
-0.10
0.00
0.10
0.20
0.30
0.40
0.50
0.60
0.70
f(x) = 0.0146564910880553 x + 0.147115256717212R² = 0.870122450590258f(x) = 0.0172369646182495 x + 0.0495494147379622R² = 0.929418287884712f(x) = 0.0127839851024209 x + 0.138111864857675R² = 0.939924071054232
f(x) = 0.0090599228518223 x + 0.0672569167331736R² = 0.954845756185485f(x) = 0.0113477653631285 x − 0.014945796754456R² = 0.908694781438623
Grafik Konsentrasi Glukosa terhadap Waktu
0.10%Linear (0.10%)0.20%Linear (0.20%)0.30%Linear (0.30%)0.40%
Waktu (menit)
Kons
entr
asi G
luko
sa
Tabel 4. SlopeS (Konsentrasi Amilum) % 1/S V 1/V
0.1 10.00 0.0113 88.500.2 5.00 0.0091 109.890.3 3.33 0.0128 78.130.4 2.50 0.0172 58.140.5 2.00 0.0147 68.03
1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.0011.000.00
20.00
40.00
60.00
80.00
100.00
120.00
f(x) = 3.26291878728677 x + 65.6348237139418R² = 0.28202175473797
Hubungan 1/V terhadap 1/S
Hubungan 1/V terhadap 1/S
Linear (Hubungan 1/V terhadap 1/S)
1/S
1/V
Hasil NilaiVmax 0.015235774Km 0.049712806