penelitian strategis nasional · untuk pembentukan galur jagung provitamin a tim peneliti ... among...
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LAPORAN
PENELITIAN STRATEGIS NASIONAL
KERAGAMAN GENETIK GENERASI 1 SELFING JAGUNG
LOKAL SULAWESI SELATAN BERDASARKAN
MARKA SIMPLE SEQUENCE REPEAT (SSR)
UNTUK PEMBENTUKAN GALUR
JAGUNG PROVITAMIN A
TIM PENELITI
DR. JUHRIAH, M.Si. NIDN 0031126308 (KETUA)
DR. A. MASNIAWATI, S.Si, M.Si. NIDN 0013027003 (ANGGOTA)
PROF. DR. IR. MIR ALAM, M.Si. NIDN 0031126306 (ANGGOTA)
UNIVERSITAS HASANUDDIN MAKASSAR
NOVEMBER 2014
Kode/ NamaRumpunIlmu: 113 (Biologi&BioteknologiUmum)
Tema : CLIMATE CHANGE AND BIODIVERSITY
iii
GENETIC DIVERSITY 1ST
GENERATION SELFING LOCAL CORN
OF SOUTH SULAWESI BASED ON MOLECULAR MARKERS
SIMPLE SEQUENCE REPEAT (SSR) FOR DEVELOPING
A STRAIN OF PROVITAMIN A OF CORN
Juhriah1, A. Masniawati
1, Mir Alam
2
1. Biologi Departement of FMIPA, Hasanuddin University, Makassar
2. Agriculture Faculty of Indonesia Timur University, Makassar
juhriah@gmail.com
ABSTRACT
Vitamin A deficiency (VAD) is a major cause of premature death in developing nations..
Among the major cereals, yellow endosperm maize is the only crop having appreciable
amount of carotenoids with a wide range of genetic variability. Several carotinoids have
provitamin-A activity due to the presence of a vitamin structure that is a part of the overall
carotenoid compound. South Sulawesi germplasm lokal corn containing high
carotenoids and phytoene synthase gene (PSY 1) as found in the original corn CYMMYT, it
means that South Sulawesi local corn germplasm has the potential to be developed into a
genetic source for the developing of varieties of corn provit A. The research aims to analysis
genetic diversity of 1st generation of selfing local corn South Sulawesi based on Simple
Sequence Repeats markers was carried out. There are 52th
samples were analysis with 10th
Simple Sequence Repeats primers, at Molecular Laboratory the Indonesian Cereals Research
Institute (ICERI) in Maros South Sulawesi. Procedure for DNA extraction, Polymerase Chain
Reaction and Electrophoresis, followed the instruction of AMBIONET. Analysis of data
using NTSYST program. The coefficient of similarity with the Simple Matching Coefficient,
and clustering by Unweighted Pair Group Aritmathic Analysis. The smallest genetic
similarity coefficient (0.48) between L3 with F1, F3 and F4 (Srikandi kuning 1 individual 3
with 3 individual Kandora local corn from Tana Toraja at South Sulawesi) and J3. while the
highest similarity value (1.0) between J3 with F1, F3 and F4 (Kui caroteniod Syn individual 3
corn from CIMMYT with 3 individual Kandora local corn from Tana Toraja). Dendrogram
showed that at 0.72 the degree of similarity, the 52 individuals that form seven groups,
there were two groups containing only one individual.
Keywords: genetic diversity, corn, Sulawesi Selatan, CIMMYT, Simple Sequence Repeats
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