lampiran 1 surat keterangan determinasi tanaman jambu...

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Lampiran 1. Surat Keterangan Determinasi Tanaman Jambu Biji

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Lampiran 1. Lanjutan

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Lampiran 2. Surat Keterangan Telah Melakukan Penelitian dari Bagian

Biologi Farmasi Fakultas Farmasi Universitas Wahid Hasyim

Semarang

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Teknologi Farmasi Fakultas Farmasi Universitas Wahid

Hasyim Semarang

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Farmakologi dan Farmasi Klinik Fakultas Farmasi

Universitas Wahid Hasyim Semarang

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Lampiran 5. Surat Keterangan Telah Melakukan Pembelian Tikus Putih

(Rattus Norvegicus) Galur Wistar usia 2-3 bulan, Jenis

Kelamin Jantan Sebanyak 25 Ekor.

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Lampiran 6. Surat Keterangan Ethical Clearance Fakultas Kedokteran

Universitas Islam Sultan Agung

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Lampiran 7. Surat Keterangan Diagnosa Dokter Rumah Sakit Islam Sultan

Agung

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Lampiran 8. Perhitungan Rendemen Ekstrak Etanol Daun Jambu Biji

Diketahui : bobot simplisia daun jambu biji = 2000 gram

Bobot ekstrak kental = 515 gram

Ditanya : randemen daun jambu biji ?

Jawab :

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Lampiran 9. Perhitungan Nilai Bahan Pembuatan Gel EEDJB

Bahan Konsentrasi sediaan (b/b)

F1 F2 F3 F4

Ekstrak daun jambu biji (g)

Karbopol 934 (g)

Gliserin (mL)

Metil paraben (g)

Propil paraben (g)

Triethanolamine (TEA) (mL)

Akuades (mL) ad

3

1

2

0,2

0,2

1,25

100

5

1

2

0,2

0,2

1,25

100

7

1

2

0,2

0,2

1,25

100

-

1

2

0,2

0,2

1,25

100

Formula 1


Karbopol 934 (g)

Gliserin (mL)

Metil paraben (g)

Propil paraben (g)

Triethanolamine (mL)

Akuades ad 100 (mL)

3

1

2

0,2

0,2

1,25 +

7,65

100-7,65 = 92,35 ≈ 92 mL

Formula 2


Karbopol 934 (g)

Gliserin (mL)

Metil paraben (g)

Propil paraben (g)


Akuades ad 100 (mL)

5

1

2

0,2

0,2

1,25 +

9,65

100-9,65 = 90,35 ≈ 90 mL

Formula 3


Karbopol 934 (g)

Gliserin (mL)

Metil paraben (g)

Propil paraben (g)


Akuades ad 100 (mL)

7

1

2

0,2

0,2

1,25 +

11,65

100-11,65 = 88,35 ≈ 88 mL

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Formula 4


Karbopol 934 (g)

Gliserin (mL)

Metil paraben (g)

Propil paraben (g)


Akuades ad 100 (mL)

-

1

2

0,2

0,2

1,25 +

4,25

100-4,25 = 95,75 ≈ 96 mL

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Lampiran 10. Hasil Pengujian Sifat Fisika dan Kimia Sediaan Gel EEDJB

1. Hasil Pengukuran Viskositas

Replikasi Viskositas Gel EEDJB (dPa.S)

F1 F2 F3 F4

1 200 200 200 150

2 200 200 200 150

3 200 200 200 150

Rata – Rata 200 200 200 200

Keterangan :

Formula 1 : Kelompok 1 gel ekstrak etanol daun jambu biji 3%



Formula 4 : Kelompok kontrol negatif basis gel karbopol

2. Hasil Pengukuran Daya Sebar

Formula Pengukuran Diameter Daya Sebar (cm) dengan Beban Diameter

Rata – Rata (cm) Kaca 50 100 150 200 250 300

F1

R1 3,2 3,3 3,4 3,5 3,6 3,7 3,8 3,50

R2 3,2 3,3 3,4 3,5 3,6 3,6 3,7 3,47

R3 3,3 3,4 3,6 3,7 3,8 3,9 4,0 3,67

Rata – Rata 3,54

F2

R1 3,2 3,3 3,4 3,5 3,6 3,7 3,8 3,50

R2 3,1 3,2 3,3 3,4 3,5 3,6 3,6 3,38

R3 3,1 3,2 3,3 3,4 3,5 3,6 3,7 3,40

Rata – Rata 3,43

F3

R1 3,0 3,1 3,2 3,3 3,4 3,5 3,5 3,28

R2 3,1 3,2 3,3 3,4 3,5 3,6 3,6 3,38

R3 3,0 3,1 3,2 3,3 3,4 3,5 3,6 3,30

Rata – Rata 3,32

F4

R1 4,4 4,5 4,6 4,7 4,8 4,9 5,0 4,70

R2 4,6 4,7 4,8 4,9 5,0 5,0 5,1 4,87

R3 4,5 4,6 4,7 4,8 4,9 5,0 5,1 5,23

Rata – Rata 4,93

Keterangan :





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3. Hasil Pengukuran Daya Lekat

Replikasi Daya Lekat Gel EEDJB (detik)

F1 F2 F3 F4

1 2,36 2,85 3,55 1,83

2 2,33 2,88 3,43 1,57

3 2,41 2,55 3,30 1,56

Rata – Rata 2,37 2,76 3,42 1,66

Keterangan :





4. Hasil Pengukuran pH

Replikasi Derajat Keasaman Gel EEDJB (pH)

F1 F2 F3 F4

1 6,99 6,66 6.58 7,15

2 6,99 6,66 6,58 7,15 3 6,99 6,66 6,58 7,15

Rata – Rata 6,99 6,66 6,58 7,15

Keterangan :





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Lampiran 11. Perhitungan Persentase Penyembuhan Luka Bakar

Perhitungan Persentase Penyembuhan Luka Bakar

Px = dx(1)2-dx(17)

2 X 100%

dx(1)2

Keterangan :

Px = persentase penyembuhan luka bakar pada hari ke 17

dx(1) = diatemer luka bakar hari pertama

dx(17) = diameter luka bakar hari ke 17

K1 EEDJB 3%

Tikus 1 Tikus 2 Tikus 3

Px = 22-0,846

2 X 100 %

22

= 4-0,7157 X 100%

4

= 82,11 %

Px = 22-0,812

2 X 100 %

22

= 4-0,6593 X 100%

4

= 83,51 %

Px = 22-0,861

2 X 100 %

22

= 4-0,7413 X 100%

4

= 81,47 %

Tikus 4 Tikus 5

Px = 22-0,911

2 X 100 %

22

= 4-0,8299 X 100%

4

= 79,25 %

Px = 22-0,961

2 X 100 %

22

= 4-0,9235 X 100%

4

= 79,91 %

K2 EEDJB 5%


Px = 22-0,463

2 X 100 %

22

= 4-0,2144 X 100%

4

= 94,64 %

Px = 22-0,47

2 X 100 %

22

= 4-0,2209 X 100%

4

= 94,48 %

Px = 22-0,44

2 X 100 %

22

= 4-0,1936 X 100%

4

= 95,16 %

Tikus 4 Tikus 5

Px = 22-0,512

2 X 100 %

22

= 4-0,2621 X 100%

4

= 93,45 %

Px = 22-0,709

2 X 100 %

22

= 4-0,5027 X 100%

4

= 87,43 %

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K3 EEDJB 7%


Px = 22-0,237

2 X 100 %

22

= 4-0,0562 X 100%

4

= 98,59 %

Px = 22-0,287

2 X 100 %

22

= 4-0,0824 X 100%

4

= 97,94 %

Px = 22-0

2 X 100 %

22

= 4-0 X 100%

4

= 100 %

Tikus 4 Tikus 5

Px = 22-0

2 X 100 %

22

= 4-0 X 100%

4

= 100 %

Px = 22-0

2 X 100 %

22

= 4-0 X 100%

4

= 100 %

K- Basis Gel Karbopol


Px = 22-1,638

2 X 100 %

22

= 4-2,6830 X 100%

4

= 32,92 %

Px = 22-1,682

2 X 100 %

22

= 4-2,8291 X 100%

4

= 29,27 %

Px = 22-1,595

2 X 100 %

22

= 4-2,5440 X 100%

4

= 36,4 %

Tikus 4 Tikus 5

Px = 22-1,566

2 X 100 %

22

= 4-2,4523 X 100%

4

= 38,69 %

Px = 22-1,565

2 X 100 %

22

= 4-2,4492 X 100%

4

= 38,77 %

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K+ Bioplacenton


Px = 22-0

2 X 100 %

22

= 4-0 X 100%

4

= 100 %

Px = 22-0

2 X 100 %

22

= 4-0 X 100%

4

= 100 %

Px = 22-0,165

2 X 100 %

22

= 4-0,0272 X 100%

4

= 99,32 %

Tikus 4 Tikus 5

Px = 22-0,467

2 X 100 %

22

= 4-0,2181 X 100%

4

= 94,55 %

Px = 22-0,535

2 X 100 %

22

= 4-0,2862 X 100%

4

= 92,84 %

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Lampiran 12. Hasil Uji Normalitas, Kruskall Walis, dan Mann Whitney

Viskositas

1. Uji Normalitas

Tests of Normality

Kolmogorov-Smirnov

a Shapiro-Wilk

Statistic Df Sig. Statistic df Sig.

Viskositas (dPa.S) ,460 12 ,000 ,552 12 ,000

a. Lilliefors Significance Correction

Uji Homogenitas

Test of Homogeneity of Variances

Viskositas (dPa.S)

Levene Statistic df1 df2 Sig.

. 3 . .

2. Uji Kruskall Walis

Ranks

Formula N Mean Rank

Viskositas (dPa.S) K1 Gel EEDJB 3% 3 8,00

K2 Gel EEDJB 5% 3 8,00


K- Basis Gel Karbopol 3 2,00

Total 12

Test Statisticsa,b

Viskositas (dPa.S)

Chi-Square 11,000

Df 3

Asymp. Sig. ,012

a. Kruskal Wallis Test

b. Grouping Variable: Formula

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3. Uji Mann Whitney

Ranks

Formula N Mean Rank Sum of Ranks

Viskositas (dPa.S) K1 Gel EEDJB 3% 3 3,50 10,50

K2 Gel EEDJB 5% 3 3,50 10,50

Total 6

Test Statisticsb

Viskositas (dPa.S)

Mann-Whitney U 4,500

Wilcoxon W 10,500

Z ,000

Asymp. Sig. (2-tailed) 1,000

Exact Sig. [2*(1-tailed Sig.)] 1,000a

a. Not corrected for ties.


Ranks



K3 Gel EEDJB 7% 3 3,50 10,50

Total 6

Test Statisticsb

Viskositas (dPa.S)


Wilcoxon W 10,500

Z ,000





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Ranks



K- Basis Gel Karbopol 3 2,00 6,00

Total 6

Test Statisticsb

Viskositas (dPa.S)

Mann-Whitney U ,000

Wilcoxon W 6,000

Z -2,236

Asymp. Sig. (2-tailed) ,025

Exact Sig. [2*(1-tailed Sig.)] ,100a



Ranks



K3 Gel EEDJB 7% 3 3,50 10,50

Total 6

Test Statisticsb

Viskositas (dPa.S)


Wilcoxon W 10,500

Z ,000





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Ranks




Total 6

Test Statisticsb

Viskositas (dPa.S)

Mann-Whitney U ,000

Wilcoxon W 6,000

Z -2,236





Ranks




Total 6

Test Statisticsb

Viskositas (dPa.S)

Mann-Whitney U ,000

Wilcoxon W 6,000

Z -2,236





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Lampiran 13. Hasil Uji Normalitas, Homogenitas, One Way Anova, dan

LSD Daya Sebar

1. Uji Normalitas

Tests of Normality

Formula

Kolmogorov-Smirnova Shapiro-Wilk

Statistic df Sig. Statistic df Sig.

Diameter Daya Sebar (cm) K1 Gel EEDJB 3% ,334 3 . ,860 3 ,266

K2 Gel EEDJB 5% ,328 3 . ,871 3 ,298

K3 Gel EEDJB 7% ,314 3 . ,893 3 ,363

K- Basis Gel Karbopol ,259 3 . ,959 3 ,610


2. Uji Homogenitas


Diameter Daya Sebar (cm)


3,729 3 8 ,061

3. Uji One Way Anova

ANOVA


Sum of Squares df Mean Square F Sig.

Between Groups 5,155 3 1,718 74,868 ,000

Within Groups ,184 8 ,023

Total 5,338 11

84


4. Uji LSD

Multiple Comparisons


LSD

(I) Formula (J) Formula

Mean

Difference (I-J) Std. Error Sig.

95% Confidence Interval

Lower Bound Upper Bound

K1 Gel EEDJB 3% K2 Gel EEDJB 5% ,12000 ,12369 ,360 -,1652 ,4052

K3 Gel EEDJB 7% ,22667 ,12369 ,104 -,0586 ,5119

K- Basis Gel Karbopol -1,38667* ,12369 ,000 -1,6719 -1,1014

K2 Gel EEDJB 5% K1 Gel EEDJB 3% -,12000 ,12369 ,360 -,4052 ,1652

K3 Gel EEDJB 7% ,10667 ,12369 ,414 -,1786 ,3919


K3 Gel EEDJB 7% K1 Gel EEDJB 3% -,22667 ,12369 ,104 -,5119 ,0586

K2 Gel EEDJB 5% -,10667 ,12369 ,414 -,3919 ,1786


K- Basis Gel Karbopol K1 Gel EEDJB 3% 1,38667* ,12369 ,000 1,1014 1,6719

K2 Gel EEDJB 5% 1,50667* ,12369 ,000 1,2214 1,7919

K3 Gel EEDJB 7% 1,61333* ,12369 ,000 1,3281 1,8986

*. The mean difference is significant at the 0.05 level.

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Lampiran 14. Hasil Uji Normalitas, Homogenitas, One Way Anova, dan

LSD Daya Lekat

1. Uji Normalitas

Tests of Normality

Formula



Daya Lekat (detik) K1 Gel EEDJB 3% ,232 3 . ,980 3 ,726

K2 Gel EEDJB 5% ,356 3 . ,818 3 ,157

K3 Gel EEDJB 7% ,178 3 . ,999 3 ,956

K- Basis Gel Karbopol ,374 3 . ,778 3 ,062


2. Uji Homogenitas


Daya Lekat (detik)


2,366 3 8 ,147

3. Uji One Way Anova

ANOVA

Daya Lekat (detik)

Sum of Squares Df Mean Square F Sig.

Between Groups 4,951 3 1,650 89,203 ,000

Within Groups ,148 8 ,019

Total 5,099 11

86


4. Uji LSD

Multiple Comparisons

Daya Lekat (detik)

LSD

(I) Formula (J) Formula

Mean

Difference (I-J) Std. Error Sig.

95% Confidence Interval

Lower Bound Upper Bound

K1 Gel EEDJB 3% K2 Gel EEDJB 5% -,39333* ,11106 ,008 -,6494 -,1372

K3 Gel EEDJB 7% -1,06000* ,11106 ,000 -1,3161 -,8039

K- Basis Gel Karbopol ,71333* ,11106 ,000 ,4572 ,9694

K2 Gel EEDJB 5% K1 Gel EEDJB 3% ,39333* ,11106 ,008 ,1372 ,6494

K3 Gel EEDJB 7% -,66667* ,11106 ,000 -,9228 -,4106

K- Basis Gel Karbopol 1,10667* ,11106 ,000 ,8506 1,3628

K3 Gel EEDJB 7% K1 Gel EEDJB 3% 1,06000* ,11106 ,000 ,8039 1,3161

K2 Gel EEDJB 5% ,66667* ,11106 ,000 ,4106 ,9228

K- Basis Gel Karbopol 1,77333* ,11106 ,000 1,5172 2,0294

K- Basis Gel Karbopol K1 Gel EEDJB 3% -,71333* ,11106 ,000 -,9694 -,4572

K2 Gel EEDJB 5% -1,10667* ,11106 ,000 -1,3628 -,8506

K3 Gel EEDJB 7% -1,77333* ,11106 ,000 -2,0294 -1,5172

*. The mean difference is significant at the 0.05 level.

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Lampiran 15. Hasil Uji Normalitas, Kruskall Walis, dan Mann Whitney pH

1. Uji Normalitas

Tests of Normality

Kolmogorov-Smirnov

a Shapiro-Wilk

Statistic df Sig. Statistic df Sig.

pH ,276 12 ,012 ,809 12 ,012


Uji Homogenitas


pH


. 3 . .


Ranks

Formula N Mean Rank

pH K1 Gel EEDJB 3% 3 8,00




Total 12

Test Statisticsa,b

pH

Chi-Square 11,000

df 3

Asymp. Sig. ,012


b. Grouping Variable:

Formula

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3. Uji Mann Whitney

Ranks


pH K1 Gel EEDJB 3% 3 5,00 15,00

K2 Gel EEDJB 5% 3 2,00 6,00

Total 6

Test Statisticsb

pH

Mann-Whitney U ,000

Wilcoxon W 6,000

Z -2,236





Ranks


pH K1 Gel EEDJB 3% 3 5,00 15,00

K3 Gel EEDJB 7% 3 2,00 6,00

Total 6

Test Statisticsb

pH

Mann-Whitney U ,000

Wilcoxon W 6,000

Z -2,236





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Ranks


pH K1 Gel EEDJB 3% 3 2,00 6,00


Total 6

Test Statisticsb

pH

Mann-Whitney U ,000

Wilcoxon W 6,000

Z -2,236





Ranks


pH K2 Gel EEDJB 5% 3 5,00 15,00

K3 Gel EEDJB 7% 3 2,00 6,00

Total 6

Test Statisticsb

pH

Mann-Whitney U ,000

Wilcoxon W 6,000

Z -2,236





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Ranks


pH K2 Gel EEDJB 5% 3 2,00 6,00


Total 6

Test Statisticsb

pH

Mann-Whitney U ,000

Wilcoxon W 6,000

Z -2,236





Ranks


pH K3 Gel EEDJB 7% 3 2,00 6,00


Total 6

Test Statisticsb

pH

Mann-Whitney U ,000

Wilcoxon W 6,000

Z -2,236





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Lampiran 16. Hasil Uji Normalitas, Homogenitas, Kruskall Walis, dan Mann

Whitney Persentase Penyembuhan Luka Bakar

1. Uji Normalitas

2. Uji Homogenitas

Test of Homogeneity of Variance


Persentase Penyembuhan

Luka Bakar

Based on Mean 2,359 4 20 ,088

Based on Median ,699 4 20 ,602

Based on Median and with

adjusted df

,699 4 15,3

56

,604

Based on trimmed mean 2,080 4 20 ,122


Ranks

Kelompok Hewan Uji N Mean Rank


Luka Bakar


K2 Gel EEDJB 5% 5 14,20

K3 Gel EEDJB 7% 5 21,20


K+ Bioplacenton 5 18,60

Total 25

Tests of Normality

Kelompok Hewan Uji




Luka Bakar

K1 Gel EEDJB 3% ,224 5 ,200* ,957 5 ,787

K2 Gel EEDJB 5% ,352 5 ,042 ,722 5 ,016

K3 Gel EEDJB 7% ,361 5 ,032 ,764 5 ,040

K- Basis Gel Karbopol ,215 5 ,200* ,890 5 ,358

K+ Bioplacenton ,320 5 ,104 ,800 5 ,081


*. This is a lower bound of the true significance.

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92


Test Statisticsa,b


Luka Bakar

Chi-Square 20,934

Df 4

Asymp. Sig. ,000


b. Grouping Variable: Kelompok Hewan Uji

4. Uji Mann Whitney

Ranks

Kelompok Hewan Uji N Mean Rank Sum of Ranks


Luka Bakar

K1 Gel EEDJB 3% 5 3,00 15,00

K2 Gel EEDJB 5% 5 8,00 40,00

Total 10

Test Statisticsb


Luka Bakar

Mann-Whitney U ,000

Wilcoxon W 15,000

Z -2,611





Ranks



Luka Bakar

K1 Gel EEDJB 3% 5 3,00 15,00

K3 Gel EEDJB 7% 5 8,00 40,00

Total 10

93

93


Test Statisticsb


Luka Bakar

Mann-Whitney U ,000

Wilcoxon W 15,000

Z -2,643





Ranks



Luka Bakar

K1 Gel EEDJB 3% 5 8,00 40,00


Total 10

Test Statisticsb


Luka Bakar

Mann-Whitney U ,000

Wilcoxon W 15,000

Z -2,611





Ranks



Luka Bakar

K1 Gel EEDJB 3% 5 3,00 15,00

K+ Bioplacenton 5 8,00 40,00

Total 10

94

94


Test Statisticsb


Luka Bakar

Mann-Whitney U ,000

Wilcoxon W 15,000

Z -2,619





Ranks



Luka Bakar

K2 Gel EEDJB 5% 5 3,00 15,00

K3 Gel EEDJB 7% 5 8,00 40,00

Total 10

Test Statisticsb


Luka Bakar

Mann-Whitney U ,000

Wilcoxon W 15,000

Z -2,643





Ranks



Luka Bakar

K2 Gel EEDJB 5% 5 8,00 40,00


Total 10

95

95


Test Statisticsb


Luka Bakar

Mann-Whitney U ,000

Wilcoxon W 15,000

Z -2,611





Ranks



Luka Bakar

K2 Gel EEDJB 5% 5 4,20 21,00


Total 10

Test Statisticsb


Luka Bakar


Wilcoxon W 21,000

Z -1,362





Ranks



Luka Bakar

K3 Gel EEDJB 7% 5 8,00 40,00


Total 10

96

96


Test Statisticsb


Luka Bakar

Mann-Whitney U ,000

Wilcoxon W 15,000

Z -2,643





Ranks



Luka Bakar

K3 Gel EEDJB 7% 5 6,20 31,00


Total 10

Test Statisticsb


Luka Bakar


Wilcoxon W 24,000

Z -,780





Ranks



Luka Bakar



Total 10

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97


Test Statisticsb


Luka Bakar

Mann-Whitney U ,000

Wilcoxon W 15,000

Z -2,619





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Lampiran 17. Dokumentasi Penelitian

Tempat Pengambilan Simplisia Proses Sortasi Basah

Proses Penimbangan Proses Pengeringan

Proses Sortasi Kering Pengecekan Kadar Air

99

99


Proses Penyerbukan Simplisia Serbuk Daun Jambu Biji

Penimbangan Serbuk Proses Maserasi

Proses Penyaringan Maserasi Hasil Maserat

100

100


Proses Pengentalan Ekstrak Hasil EEDJB

Proses Pembuatan Basis Gel Karbopol

Alat dan Bahan Pembuatan Gel

101

101


Proses Pembuatan Gel Penimbangan Sediaan Gel

Gel EEDJB 3% Gel EEDJB 5%

Gel EEDJB 4% Basis Gel Karbopol

102

102


Pengujian Sifat Fisika Kimia Gel

Pengujian Viskositas Menggunakan Viskometer Rion

Pengujian pH Menggunakan pH Meter

Pengujian Daya Lekat

103

103


Pengujian Daya Sebar

Pengukuran Diameter Daya Sebar

104

104


Proses Seleksi Tikus Galur Wistas Proses Aklimitasi Tikus

Sewaktu Pmbelian

Proses Pencukuran Bulu Tikus Anastesi Lokal

Proses Pembuatan Luka Bakar

Alat Solder Pembuat Luka Bakar

105

105


Kandang Tikus Individu

Proses Pengolesan Sediaan Gel EEDJB, Bioplacenton, dan Basis Gel Karbopol

106

106


Pengujian Efektivitas Luka Bakar

Kelompok 1 Gel EEDJB 3%

Sebelum (pada hari ke-0) Sesudah (pada hari ke-17)





107

107


Kelompok 4 Basis Gel Karbopol


Kelompok 5 Bioplacenton


Alat Pengukur Diameter Luka Bakar Proses Pengukuran Diameter

(Jangka Sorong) Luka Bakar

lampiran 1 surat keterangan determinasi tanaman jambu...

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