kromatografi eksklusi ukuran - sec

31
FDN D ODG NY BFD ] BL YD@J XJ UC CWH OSXJ BA HGYBFD]BLYD[GZ Mjxsxsa bocg 5 N caaz Y zd a Ojfda} b ;:74 4 =;;0 Icy ad mc} } d Dysf \! ;:744 =;;< Ydhgcojd Bh} d pjd ;:744=;;7 E caaz Fdy jad ;:744=;40 J ad Esaj Ad } dxj d ;:744=;98 Nyjx}j ad Ac}z ;:744 =;8= @DNSO]DX @DYFDXJ SAJ PCY XJ ]DX XDAD] D MGDY FD ZBLZDNDY]D 9;44

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Page 1: KROMATOGRAFI EKSKLUSI UKURAN - SEC

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FDNDODG NYBFD]BLYD@J

XJ UC CWHOSXJ BA HGYBFD]BLYD[GZ

Mjxsxsa bocg 5

Ncaaz Yzda Ojfda} b ;:744=;;0

Icy admc} } d Dysf \! ;:744=;;<

Ydhgco jd Bh} dpjd ;:744=;;7

E caaz Fdy jad ;:744=;40

J ad Esaj Ad} dxjd ;:744=;98

Ny jx} jad Ac} z ;:744=;8=

@DNSO]DX @DYFDXJ

SAJ PCYXJ ]DX XDAD]D MGDYFD

ZBLZDNDY]D

9;44

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XJUC CWHOSXJBA HGYBFD]BLYD[GZ

[CAMDGSOSDA

Nybfd}blyd`j {cy}dfd ndoj mjncfidalnda bocg xcbydal dgoj ib}daj Ysxjd Fjhgdco ]x|c}}

{dmd }dgsa 4:;8 sa}sn fcfjxdgnda {jlfca icy|dyad mdodf }dadfda mcalda hdyd {cynbodxj

cnx}ydn {c}ybocsf c}cy mdodf nbobf lcodx zdal icyjxj ndoxjsf ndyibad} 'HdHB8*! Xdd} jaj

nybfd}blyd`j fcys{dnda }cnajn {cfjxdgda zdal {dojal sfsf mda {dojal xcyjal mjlsadnda sa}sn

ijmdal njfjd dadojxjx! Nybfd}blyd`j md{d} mjfda`dd}nda sa}sn fcodnsnda dadojxjx/ idjn dadojxjx

nsdoj}d}j`/ nsda}j}d}j`/ fds{sa {yc{dyd}j` mdodf ijmdal `dyfdxj/ ojalnsalda/ jamsx}yj/ mda

xcidldjazd! Nybfd}blyd`j fcys{dnda xsd}s }cnajn {cfjxdgda zdal fcalsadnda `dxc mjdf

'x}d}jbadyz {gdxc* mda `dxc lcydn 'fbijoc {gdxc* 'Ldamedy/ 9;;<*!

]cnajn nybfd}blyd`j xcamjyj }codg mjncfidalnda mda }codg mjlsadnda sa}sn fcfjxdgnda

mda fcalnsda}j`jndxj icyidldj fdhdf nbf{baca zdal nbf{ocnx/ idjn nbf{baca byldajn

fds{sa nbf{baca dabyldajn! Nybfd}blyd`j md{d} mjicmdnda d}dx icyidldj fdhdf }cylda}sal

{dmd {calcobf{bndaazd! Icymdxdynda {dmd fcndajxfc {cfjxdgdaazd/ nybfd}blyd`j mjicmdnda

fcaedmj 'd* nybfd}blyd`j dmxbyixj> 'i* nybfd}blyd`j {dy}jxj> 'h* nybfd}blyd`j {dxdalda jba> 'm*

nybfd}blyd`j {casndy jba> 'c* nybfd}blyd`j cnxnosxj snsyda> xcy}d nybfd}blyd`j d`jaj}dx 'Ldamedy/

9;;<*!

J! NYBFD]BLYD@J CNXNOSXJ

[cfjxdgda icyidldj nbax}j}sca mcalda fcajaeds {cyicmdda snsyda mda lcbfc}yj fbocnso

dmdodg mdxdy nybfd}blyd`j cnxnosxj! [cyicmdda snsyda fcazcidinda icicyd{d {dy}jnco icylcydn

ocijg hc{d} mdyj zdal odjaazd xcgjalld fcajfisonda {cyicmdda {cyfsndda fjlydxj!

Nybfd}blyd`j cnxnosxj md{d} mjncobf{bnnda mdodf }jld nd}clbyj 5d! Nybfd}blyd`j {cyfcdxj lco d}ds ̀ jo}ydxj lco

@jo}ydxj lco dmdodg xsd}s }cnajn zdal fcalsydjnda hdf{syda ud}&ud} xcxsdj mcalda

snsyda fbocnsoazd! ]cnajn jaj mjmdxdynda d}dx janosxj mda cnxnosxj xsd}s ud} }cyodys}

fcodosj xsd}s `dxc mjdf zdal }cyisd} mdyj lco {bojfcy zdal icyjnd} xjodal mda icy{byj

gc}cyblca! Mdodf nybfd}blyd`j cosxj hdjy&{dmd}/ {cfjxdgda }cyedmj da}dyd `dxc hdjy mj mdodf

{dy}jnco lco mda hdjyda mj osdy zdal fcalcojojalj {dy}jnco lco! Dnjid} fcndajxfc {cyicmdda

odes {cyfcdxj fdxjal&fdxjal fbocnso ud} }cyodys} mdyj mda nc ja}cyjby {dy}jnco lco/

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JJ! NBOBF

Nbobf cnxnosxj fcaldamsal {dy}jnco icy{byj mcalda ldyjx }caldg {byj zdal icyicmd! Xbos}zdal mjxsa}jnnda nc mdodf nbobf dnda icymj`sxj nc mdodf {byj zdal ldyjx }caldgazd ocijg icxdy

mdyj{dmd ldyjx }caldg c`cn}j` xbos}! Ldyjx }caldg c`cn}j` mj}saesnnda {dmd ldfidy icyjns} 5

'Ngb{ndy/ 4::;*!

[cygd}jnda/ |dods{sa ibib} fbocnso xdfd/ ldyjx }caldg c`cn}j`azd icyicmd/ mda nbf{baca zdal

ldyjx }caldg c`cn}j`azd {dojal icxdy }cycosxj ocijg mdgsos! Xcodja j}s/ ldyjx }caldg c`cn}j̀  

fcahdns{ {sod fbocnso xbopdxj d{d xded 'Ngb{ndy/ 4::;*!

Mcalda icy}dfidg icxdyazd ldyjx }caldg c`cn}j` xbos}/ esfodg {byj zdal md{d} mjfdxsnjazd

mda ncfdf{sdaazd icymj`sxj nc mdodf {byj fcasysa! Edmj/ ejnd xbos} icyldyjx }caldg mcfjnjda

ys{d xcgjalld }jmdn md{d} icymj̀ sxj nc mdodf {byj zdal fdad {sa/ fdnd xbos} }cyxcis} md{d}

mjnd}dnda mjcnxnosxj xcf{syad mda }jmdn mj}dgda/ dy}jazd xbos} j}s }cycosxj mdodf pbosf fd}j 'Pb*

nbobf! Xbos} zdal fdf{s icymj`sxj xcf{syad nc mdodf xcfsd {byj mjnd}dnda icy{cyfcydxj

xcf{syad nc mdodf ncfdxda! Xbos} ecajx jaj fcfcyosnda pbosf {codys} zdal edsg ocijg icxdy

sa}sn fcalcosxjazd mdyj nbobf! [cfjxdgda nbf{baca md{d} }cyhd{dj ejnd nbf{baca j}s }cycosxj

mcalda pbosf }dfid} da}dyd Pb mda pbosf {cyfcdxj }b}do 'Ngb{ndy/ 4::;*!

Pbosf nbobf cnxnosxj }cymjyj d}dx }jld nbf{baca zdal ecodx/ zdj}s 5

4! pbosf fd}j/ Pb ‘ pbosf ysdal da}dy{dy}jnco zdal mj}c{d}j `dxc lcydn zdal fcaldojy/

9! pbosf zdal mj}cf{d}j bocg idljda {dmd} ncfdxda/

8! pbosf {byj 'P{* ‘ pbosf zdal mj}cf{d}j `dxc lcydn zdal fdamcn! [dmd nybfd}blyd`j cnxnosxj/

jaj md{d} mjxdfdnda mcalda pbosf `dxc mjdf 'Ngb{ndy/ 4::;*!

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'Ngb{ndy/ 4::;*!

Xc}jd{ ncfdxda cnxnosxj ysdal zdal icyicmd snsyda {byjazd fcf{sazdj nsypd ndojiydxj

xcamjyj! Id}dx cnxnosxj mda yca}dal ncyed ibib} fbocnso 'IF* {dmd ldfidy }jmdn mjmc`jajxjnda

mcalda }dedf ndycad mjx}yjisxj {byj ncfdxda nbobf }jmdn xcf{j}! Mjx}yjisxj {byj {dmd ncfdxda

fcaca}snda ncfjyjalda nsypd ndojiydxj! Ejnd mjx}yjisxj {byj ocidy/ nsypd fcf{sazdj ncfjyjalda

zdal }dedf! Edmj/ yca}dal ncyed IF icxdy/ }c}d{j dnda fcalgdxjonda mdzd {jxdg ycamdg {dmdxcazd|d&xcazd|d zdal snsyda fbocnsoazd gdf{jy xdfd! Ejnd mjx}yjisxj xcf{j}/ nsypd ocjg

fcamd}dy! Edmj yca}dal ncyed IF dnda ocijg nchjo/ }c}d{j mdzd {jxdg fbocnso zdal snsydaazd

gdf{jy xdfd dnda fcajalnd} 'Ngb{ndy/ 4::;*!

Nbobf zdal icyicmd yca}dal ncyed IF&azd mj{dndj sa}sn fcalgdxjonda {cfjxdgda

b{}jfsf nbf{baca hs{ojnda zdal ocidy mjx}yjisxj ibib} fbocnsoazd! ]jd{ ydalndjda nbobf

fcf{sazdj nsypd ndojiydxj xcamjyj/ zdal mj{cybocg mcalda fcazsa}jnnda hs{ojnda idns zdal

ibib} fbocnsoazd mjnc}dgsj mda fcaca}snda Py fdxjal&fdxjal! Nybfd}blyd j̀ cnxnosxj dmdodg

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fcfjojnj msd mc}cn}by mdodf ydalndjda! Sa}sn {caca}sda nbf{bxjxj dnsyd} nb{bojfcy xc}jmdnazd

msd mdyj mc}cn}by }cyxcis} gdysx mc}cn}by nbaxca}ydxj 'Xdamocy/ 4::7*! [caca}sda nbf{bxjxjnb{bojfcy zdal {dojal mjodnsnda mcalda fcallsadnda mc}cn}by SP mda YJ/ fcxnj{sa

nbfijadxj odja md{d} mjlsadnda '[dxhg/ 9;;;*!

4! Yc`ydn}by mj̀ cycaxjdo

Mc}cn}by jamcnx ijdx mjidalsa xcidldj dojyda fcodosj

yc`ydn}bfc}cy mj`cycaxjdo zdal }cysx fcacysx fcalsnsy

{cyicmdda jamcnx ijdx cosd} mda cosca fsyaj! Ldfidy 4

fcasaesnnda xncfd edosy xjady fcodosj jax}ysfca!

Hdgdzd zdal mj{dahdynda mdyj 4 fcoc|d}j hcodg OCM 9

xcicosf mjicobnnda bocg hcyfja xcfj&}ydax{dyda 8 mda

fcoja}dxj msd idljda mdyj xco/ fcalsnsy = hcyfja xcfj&

}ydax{dyda 8/ mda xcededy ijmdal {cod} ndhd 0! Dngjyazd/ xjady

mjidlj fcaedmj msd {dyxjdo idobn bocg {yjxfd <! Ja}caxj}dx

mdyj idobn mj 7d/ 7i zdal fcallsadnda `b}b mjbmd mda mjlsadnda xcidldj snsyda sa}sn {bxjxj

xjady dxoj!

'Dabajf d/ 9;44*!

9! X{cn}yb`b}bfc}cy SP

Mdodf dx{cn nsda}j}d}j`/ xsd}s icyndx ydmjdxj mjncadnda {dmd hs{ojnda 'odys}da

xdf{co* mda ja}caxj}dx xjady ydmjdxj zdal mj}cysxnda mjsnsy icxdyazd! Ydmjdxj zdal mjxcyd{

bocg hs{ojnda mj}ca}snda mcalda fcfidamjalnda ja}caxj}dx xjady zdal mj}cysxnda mcalda

ja}caxj}dx xjady zdal mjxcyd{ ejnd }jmdn dmd x{cxjcx {cazcyd{ odjaazd! Ja}caxj}dx d}ds

ncnsd}da ydmjdxj hdgdzd xcidamjal mcalda esfodg `b}ba zdal fcodosj xd}s xd}sda osdx

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[CFIDGDXDA ESYADO 'Lyda}/ O!D!/ Bx}caxba/ D!F!/ mda [! Ydzdx&Msdy}c/ [!/ 9;;9*!

Mc}cyfjadxj ndamsalda dfjobxd mda dfjob{cn}ja mdodf {d}j ldamsal fcallsadnda

G[XCH

Xcesfodg fc}bmc }codg mjncfidalnda sa}sn {caca}sda {cyxca ndamsalda dfjobxd mdyj

{d}j xcycdo! Mcalda fcallsadnda x{cn}yb`b}bfc}yj '\joojdfx c} do 4:<;> Fbyyjxba mda Odjlacoc}

4:78> Nas}xba 4:70> Hgydx}jo 4:7<> Edypjx mda \doncy 4::8> Fdy}jacu mda [ybmboojc} 4::0*

fcys{dnda fc}bmc zdal {dojal xcyjal mjlsadnda/ }c}d{j idys&idys jaj fc}bmc mj`cycaxjdo

xhdaajal ndobyjfc}yj 'MXH* 'Xjcpcy} mda Gbof 4::8> Fcx}ycx c} do 4::0*/ G[XCH 'Nbidzdxgj c}

do 4:73> Ncaacmz c} do 4::9> @odffc c} do 4::=> Id}cz mda Hsy}ja 4::0* mda xchdyd caujfd}jn 

{cahcyadda 'Xdylcda} 4:79> Fd}gcxba mda \coxg 4:77> Zsa mda Fd}cxba 4::;* }codg

mjncfidalnda! Mcalda fdxjal&fdxjal fc}bmc/ {cyxjd{da xdf{co/ dadojxjx zdal mjlsadnda/ mda

gdxjo zdal mj{cybocg edsg icyicmd! Fc}bmc G[XCH mjncfidalnda mda mjsej {dmd icyidldj xcycdo

{d}j sa}sn {cfjxdgda mda {caca}sda {cyxca dfjobxd mda dfjob{cn}ja! Fc}bmc zdal mjecodxnda

fcallsadnda NBG 4/; F/ 0!; F sycd/ mda :; fcaj} {dmd {cfdadxda 4;; »H sa}sn icady&icady

fcodys}nda {d}j! Dadojxjx jaj fcallsadnda djy xsojal mcjbajxdxj xcidldj cosda}/ mda {cfjxdgda

dfjobxd mda dfjob{cn}ja mjhd{dj mdodf |dn}s :; fcaj} fcallsadnda xd}s nbobf!

Idgda mda fc}bmc

Xdf{co

[d}j mjjxbodxj mdyj mdyj }sesg xdf{co mdyj ldamsf mcalda ldosy gdym ycm x{yjal 'GYX* mda

cf{d} xdf{co ldamsf mcalda ldosy msysf! Sa}sn {yc{dydxj xdf{co/ mjlsadnda fc}bmc Lyda}

'4::7*! Dfjobxd mda dfjob{cn}ja mjjxbodxj mdyj ldamsf {d}j hp! Oca GYX fcasys} fc}bmc

Fba}lbfcyz mda Xca}j '4:37*! Dfjobxd nca}dal 'D;349* ']j{c JJJ* mda dfjob{cn}ja ':<03*

mj{cybocg mdyj Xjlfd Hgcfjhdo 'X} Obsjx/ FB*!

[cazjd{da Xdf{co

[d}j zdal }cyjxbodxj mjgjodalnda ocfdnazd xcodfd 40 edf fcallsadnda fc}dabo mda dod}

cnx}ydnxj Xbwgoc}! Adfsa/ {calgjodalda ocfdn jaj ncfsmjda }cyisn}j }jmdn mj{cyosnda sa}sn 

G[XCH 'Liy! 4*!

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:; 4/043 9/=<9 =/;7< 8:!3 ́ ;!< 0;!3 ́ ;!<

49; 4/774 9/;=0 8/:9< =9!: ́ 4!< 39!4 ́ 4!<

Dfjob{cn}ja

8; 4/4=7 94; 4/837 7=!3 ́ ;!4 43!3 ́ ;!4

0; 9/039 344 8/408 78!: ́ ;!4 40!4 ́ ;!4

:; 8/:<; <<= =/<== 78!7 ́ ;!: 40!9 ́ ;!:

49; 8/073 <48 =/8:7 78!: ́ ;!0 40!4 ́ ;!0

d! Ycyd}d mda x}damdy mcpjdxj

i! Saj} jamcnx ijdx

]DICO JJJ

Dfjobxd mda Dfjob{cn}ja mdyj Gdym Ycm X{yjal 'GYX*/ Msysf/ mda

Abyfdo Odja xcy}d [d}j Ojoja fcallsadnda G[XCH

Xdf{co [d}j Dfjobxd '#* Dfjob{cn}ja '#*

GYX

Lydamja 93!; ´ 8!3 <3!; ´ 8!3

Los{yb 90!; ´ 4!: <=!; ´ 4!4

[ybx{ch} 93!; ´ 4!: <3!; ´ 4!:

Oca 90!; ´ 4!9 <=!; ´ 4!9

X}bd 9<!; ´ 4!7 <8!; ´ 4!7Is}}c 70 9=!; ´ 4!4 <0!; ´ 4!4

Fdyxgdoo 93!; ´ 9!8 <3!; ´ 9!8

Msysf

Pjh 90!; ´ 4!4 <=!; ´ 4!4

\dym 9=!; ´ 4!4 <0!; ´ 4!4

Fbaybc 93!; ´ 9!0 <3!; ´ 9!0

Hdamb 98!; ´ 4!7 <<!; ´ 4!7

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[d}j odjaazd

Edlsal 9;!4 ´ 4!0 <:!: ´ 4!0

Nca}dal 9<!: ´ 9!4 <9!4 ´ 4!0

Icydx 4:!8 ´ 4!: 7;!< ´ 4!:

Ojoja msysf ;!; ´ ;!; 4;;!; ´ ;!;

Ojoja edlsal ;!; ́ ;!; 4;;!; ́ ;!;

%fcda mda x}damdy mcpjdxj mjmd{d}nda mdyj md}d mcalda }jld ndoj yc{ojndxj

Xdf{co [d}j Ldamsf

]dico JJJ fcasaesnnda ndmdy dfjobxd mda dfjob{cn}ja xcy}d x}damdy mcpjdxj xdf{co GYX

mda {d}j ldamsf msysf zdal mdodf {cyhbiddaazd mjodnsnda }jld ndoj yc{ojndxj! Ajodj nbc`jxjca

pdyjdxjazd 'HP* dmdodg 4/=&=/<#! Esfodg fdxjal&fdxjal dfjobxd mda dfjob{cn}ja dmdodg 98&

9<# mda <8&<<#! Ajodj&ajodj zdal mjmd{d} jaj }codg xcxsdj mcalda {cyhbidda zdal xcicosfazd

}codg mjodnsnda Fcmhdo` mda Ljoocx '4:03* mcalda fc}bmc zdal icyicmd! Fc}bmc G[XCH esld

mjsej mcalda }c{sal xcycdo odjaazd xcodja {d}j ldamsf! Mdyj }dico JJJ mj}saesnnda ajodj yd}d yd}d

mda x}damdy mcpjdxj sa}sn edlsal/ nca}dal/ icydx/ mda msd ojoja {d}j 'edlsal mda ldamsf msysf*!

Mcalda fcallsadnda G[XCH/ }cyojgd} idg|d {d}j ojoja fcaldamsal dfjob{cn}ja 4;;#/

xcmdalnda {dmd {d}j abyfdo }cyndamsal esfodg yd}d&yd}d dfjobxd mda dfjob{cn}ja zdal

mjod{bynda mdodf oj}cyd}sy!

Ncsa}salda Fc}bmc

Dmd }jld ncsa}salda s}dfd fcallsadnda fc}bmc G[XCH sa}sn {caca}sda {cyxca

ndmdy dfjobxd mda dfjob{cn}ja! 4* Dnsydxj! Mcalda fcallsadnda G[XCH/ md{d} mj}ca}snda

 esfodg {cyxca ndamsalda sa}sn xcfsd ecajx {d}j zdal mjsejnda! Xcmdalnda sa}sn fc}bmcodjaazd/ gdazd md{d} fcficyjnda cx}jfdxj ndamsalda dfjobxd {dmd xdf{co! 9* Ncxcodfd}da!

[dmd fc}bmc G[XCH/ xdf{co mjxdyjal fcallsadnda djy xsojal zdal }codg mj&mcjbajdxj! Djy jaj

ncfsmjda mjlsadnda esld xcidldj cosda}! Xcmdalnda {dmd fc}bmc odjaazd/ mjlsadnda odys}da

idxd d}ds{sa ndyxjablcajn sa}sn fcodys}nda {d}j mda xcidldj cosda} 8* C`jxjcaxj \dn}s! [dmd

dadojxjx mcalda fcallsadnda fc}bmc odjaazd/ {cyos mjodnsnda mc`d}}jxdxj/ {calsadda sycd sa}sn 

fcajalnd}da ncodys}da {d}j/ mda {callsadda nbobf zdal xdald} }jmdn c`jxjca {callsaddaazd!

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3

[caca}sda Dfjobxd mda Dfjob{cn}ja {dmd X}dyhg Ldamsf Fcallsadnda

Gjlg [cy`byfdahc Xjuc&Cwhosxjba Hgybfd}blyd{gz 'G[XCH*

Idazdn fc}bmc }codg mjncfidalnda sa}sn {caca}sda ndmdy {cyxca dfjobxd mdyj {d}j xcycdo!

Fcycnd fcallsadnda x{cn}yb`b}bfc}cy '\joojdfx c} do 4:<;> Fbyyjxba mda Odjlacoc} 4:78>

Nas}xba 4:70> Hgydx}jo 4:7<> Edypjx mda \doncy 4::8> Fdy}jacu mda [ybmboojc} 4::0*

fsalnja dmdodg {dojal idazdn mjlsadnda/ }c}d{j fc}bmc idys fcallsadnda }cyfblydf

xhdaajal 'MXH* 'Xjcpcy} mda Gbof 4::8> Fcx}ycx c} do 4::0*/ G[XCH 'Nbidzdxgj c} do 4:73>

Ncaacmz c} do 4::9> Id}cz mda Hsy}ja 4::0* mda caujf {cahcyadda 'Xdylcda} 4:79>> @odffc

c} do 4::= Fd}gcxba mda \coxg 4:77> Zsa mda Fd}cxba 4::;* }codg mjncfidalnda! Mcalda

fdxjalfdxjal fc}bmc {cyxjd{da xdf{co/ xbosxj zdal mjlsadnda mda gdxjoazd xdald}

icyicmd! G[XCH fc}bmc mjncfidalnda mda mjsej mdyj {d}j xcycdo zdal icyicmd sa}sn 

{cfjxdgda mda {caca}sda dfjobxd mda dfjob{cn}ja! Fc}bmc zdal mjecodxnda fcallsadnda

NBG 4/; F/ 0/; f sycd mda :; fcaj} {dmd {cfdadxda 4;; »H sa}sn icadyicady fcodys}nda

{d}j! Dadojxjx jaj fcallsadnda mcjbajxdxj djy xsojal xcidldj cosca mda {cfjxdgda dfjobxd

mda dfjob{cn}ja mjodnsnda mdodf |dn}s :; fcaj} mcalda fcallsadnda nbobf }salldo!

FD]CYJDO MDA FC]BMC

Xdf{co

[d}j/ }cyjxbodxj mdyj }sesg fd}d fcydg 'GYX* mda ldamsf msysf nso}jpdy cf{d}/ mjxsxsa

mcalda fcallsadnda fc}bmc Lyda} '4::7*! Dfjobxd mda dfjob{cn}ja mjjxbodxj mdyj {d}j

ldamsf nso}jpdy GYX xcxsdj mcalda fc}bmc Fba}lbfcyz mda Xca}j '4:37*! Nca}dal dfjobxd

'D;349* ']j{c JJJ* mda dfjob{cn}ja ':<03* mj{cybocg mdyj Xjlfd Hgcfjhdo 'X} Obsjx/ FB*!

[cazjd{da Xdf{co

X}dyhg zdal xsmdg mjjxbodxj mjmc`d}jal xcodfd 40 edf fcallsadnda fc}gdabo mda dod}

cnx}ydnxj Xbwgoc}! Adfsa/ mc`d}jal }cyazd}d }jmdn mj{cyosnda sa}sn G[XCH 'liy 4*! X}dyhg

'9; fl* mjodys}nda mcalda {cadfidgda =/3 fO NBG 4!;F mda ;!3 fO sycd 0!;F mda

fcfdadxndaazd {dmd xsgs 4;;»H/ mcalda aj}yblca xcodfd :; fcaj} 'Fbyyjxba mda Odjlacoc}

4:78*! Xc}codg {cfdadxda/ 4 fO xdf{co mjac}ydonda mcalda 4/; F GHo mda mjxdyjal fcodosj

 edysf xsa}jn ajoba=3 fjnybfc}cy / mjdfc}cy 48 ff/ xcicosf dadojxjx gjmyb`jojn!

Gjlg&[cy`byfdahc Xjuc&Cwhosxjba Hgybfd}blyd{gz 'G[XCH*

Dfjobxd mda dfjob{cn}ja mj{jxdgnda {dmd nbobf \d}cyx So}ydgzmyblco Ojacdy 0‘48

fjnybfc}cy/ <!7 w8;;ff mda nbobf so}ydgzmyblco lsdym '\d}cyx/ Fjo`bym/ FD*fcallsadndda Gc|oc}} [dhndym 'G[ 4;:;* gjlg{cy`byfdahc ojtsjm hgybfd}blyd{g 'Dljoca}

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]chgabobljcx/ \jofjal}ba/ MC*/mjxjd{nda mcalda ds}bxdf{ocy! Mc}ch}by jamcnx yc`ydn}j` 

Gc|oc}} [dhndym4;=<D mda [H |j}g hgcfx}d}jba 'G[ gcfXd}jba `by OH Ycp! D!;=!;4*

mjlsadnda sa}sn hba}ybo mda ja}clydxj! Xcosysg xdf{co mjdadojxjx {dmd xsgs =3»H mcalda djy

zdal }cymcx}ojdxj/}cymcjbajxdxj/ }cyxdyjal xcidldj cosca}! @ob| yd}c ;!8 fO"fja mda pbosfc

jaecnxj 9; fnyboj}cy!

GDXJO MDA [CFIDGDXDA

[yc{dydxj Xdf{co

Dfjobxd mda dfjob{cn}ja {dmd ldamsf }jmdn fsmdg odys} {dmd xsgs ndfdy xcidjn dfjobxd

mda dfjob{cn}ja {dmd nca}dal/ ndycad j}s/ {cfdadxda mjis}sgnda/ mjns}j{ mdyj Fbyyjxba mda

Odjlacoc} '4:78*!]dico 4 fcasaesnnda |dn}s {cfdadxda b{}jfsf zdal mjis}sgnda bocg

x}dyhg dfjobxd mda dfjob{cn}ja sa}sn fcajalnd}nda ncodys}da! \dn}s {cfdadxda b{}jfsf

mj}ca}snda sa}sn }jd{ `ydnxj mcalda fcalgj}sal }b}do dycd {dmd `ydnxj {dy}jnsody! Dycd zdal

mj}saesnnda {dmd {cfdadxda 8;/ 0;/ :;/ mda 49; fcaj}! Mdcydg {cdn b{}jfsf sa}sn `ydnxj

dfjobxd mjhd{dj mcalda {cfdadxda 8; fcaj} '=79; yc`ydh}jpc jamcw saj} ^YJSV*/ }c}d{j dycd

{cdn b{}jfsf fcfis}sgnda :; fcaj} sa}sn `ydnxj dfjob{cn}ja '=<== YJS*! [cyxca}dxc

dfjobxd mda dfjob{cn}ja zdal }cyxjxd gdf{jy xdfd {dmd {cfdadxda 8;/ 0;/ :; fcaj} sa}sn 

dfjobxd mda gdf{jy xdfd {dmd {cfdadxda 0;/ :;/ 49; fcaj} sa}sn dfjob{cn}ja! Ndycad j}s

{cfdadxda :; fcaj} mj{jojg sa}sn xcfsd xdf{co x}dyhg dldy fcaedfja ncodys}da xcosysg

dfjob{cn}ja!

[dmd {ybxcmsy mdyj Fbyyjxba mda Odjlacoc} '4:78*/ ;/3 fO sycd 0/; F mjlsadnda sa}sn 

fcfida}s ncodys}da! Ndfj fcodnsnda xcisdg {cyhbidda sa}sn fcojgd} ijod sycd mj{cyosnda

sa}sn fc}bmc G[XCH! ]dico JJ fcasaesnnda c`cn sycd zdal mjlsadnda {dmd GYX x}dyhg

ldamsf {dmd {cfdadxda 8;/ 0;/ :;/ mda 49; fcaj}! Sa}sn xcfsd |dn}s {cfdadxda/ md}d

fcasaesnnda osdx dycd {cdn zdal ocijg icxdy {dmd xdf{co mcalda {cadfidgda sycd! Md}d jaj

fcasaesnnda nclsadda {cadfidgda sycd {dmd ncodys}da xdf{co x}dyhg! Ncsa}salda odja mdyj

sycd fcfsahsonda xcisdg c`cn ncx}dijoda! Sycd fcfchdg jnd}da gjmyblca mda ocijg x}dijo

{cazcxsdjda ica}snazd mcalda dmdazd sycd! Xdf{co x}dyhg mcalda {cadfidgda sycd '}dico J*

fcf{cy}dgdanda dycd {cdn sa}sn ncmsd dfjobxd mda dfjob{cn}ja mj fdad xdf{co x}dyhg

}da{d sycd fcasaesnnda {casysada dycd {cdn sa}sn jamjndxj mclydmdxj dfjobxd!

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Xdf{co X}dyhg Ldamsf

]dico JJJ fcasaesnda yd}dyd}d 'fcda* dfjobxd mda dfjob{cn}ja xcy}d x}damdy mcpjdxj mdyj

yc{ojndxj xcidazdn }jld ndoj GYX mda msysf {dmd xdf{co X}dyhg ldamsf! Ajodj Nbc`jxjca

Pdyjdxj 'HP* da}dyd 4/= ‘ =/< #! Icy}sy}}sys}/ esfodg dfjobxd mda dfjob{cn}ja zdj}s 989< #

mda <8<< #! Esfodg jaj }codg mjxc}sesj mcalda esfodg zdal mjod{bynda sa}sn ldamsf bocg

Fcmhdo` dam Ljoocx '4:03*! Fc}bmc G[XCH esld mjsej mcalda x}dyhg zdal odja mcalda

{cadfidgda nc X}dyhg ldamsf! ]dico JJJ esld fcasaesnda ajodj yd}dyd}d mda x}damdy mcpjdxj

sa}sn edlsal/ nca}dal/ icydx mda msd |dwz x}dyhg 'edlsal mda ldamsf msysf*! Fcodosj

G[XCH/ mcalda ecodx md}d fcasaesnnda |dwz x}dyhg fcaldamsal 4;;# dfjob{cn}ja/

xcmdalnda x}dyhg abyfdo fdaldamsal esfodg yd}dyd}d dfjobxd mda dfjob{cn}ja zdal

mjod{bynda mj oj}cyd}syc!

Ncsa}salda Fc}bmc

Dmd }jld ncsa}salda fcallsadnda fc}bmc G[XCH sa}sn fcaca}snda {cyxca dfjobxd mda

dfjob{cn}ja!

4! Ncdnsyd}da

Mcalda G[XCH/ esfodg {cyxca ncxcosysgda abyfdo mda |dwj hcycdo x}dyhg md{d}

mj}c}d{nda! Fc}bmc {caldfd}da odja fcasaesnnda ajodj ndamsalda dfjobxd!

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