7/25/2019 Ikan Zebra

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Ikan zebra, Danio rerio, merupakan spesies vertebrata yang awalnya digunakan

untuk mempelajari biologi perkembangan dan embriogenesis.

Embrio spesies memiliki kesamaan dengan manusia dalam segi mekanisme

perkembangan molekular dan fsiologi sel.

Spesies kini muncul sebagai model studi pathogen meliputi pengujian aktivitasserta potensi zat sebagai obat dan antikanker yang lebih efsien dibandingkan

model uji menggunakan binatang pengerat.

otensi dapat dinilai secara visual dengan melihat mutagenesis organ internal

embrio spesies, hasil interaksi zat obat dan embrio pada konsentrasi letal tertentu.

!rgan yang dapat dipengaruhi oleh zat antara lain otak, hati, sistem kardiovaskular,

tulang rawan, pancreas, usus, dan ginjal "#ari et al $%%&'

#ari (, )odeck *, Dicker +. $%%&. ebrafsh- +n Emerging odel System /or 0uman

Disease and Drug Discovery. 1linical harmacology 2 3herapeutics. 4ol 5$ 6o 7.0lm- &%85%.

$.&. Embryo to9icity test

 3he zebrasfsh embryo to9icity test was carried out according to the method

described by!liveira et al. "$%%:'. 6ormally /ertilized eggs at ;8 to 58cell stage were

selected /or the test. 3hey were cul8tured in $;8well microplates at $5.<1 with one

egg per well and $ ml )inger=s solution. >irstly, the lethality to zebrafsh embryos

e9posed to varied concentrations o/ sediment e9tracts o/ ?1, @ and !S1 /or $; h

was e9amined. 3hen the teratogenic and lethal e/8/ects on zebrafsh embryos

e9posed to <, $% and $%% mg dry weightAml sediment e9tracts o/ ?1, @ and !S1,

respectively, which induced closely similar cytoto9icity in the >( cells, werecompared. + total o/ $% eggs was used /or each treatment. 3he development o/

embryos /rom cleavage to early larval stages was monitored at specifed time points

"7.<, B, C, :, 7$, $;, ;5 and &$ h' a/ter /ertil8ization. Endpoints used /or assessing

the eects o/ the sediment e9tracts included embryo mortality, yolk sac edema, tail

mal/orma8tion, changes in hatching rates and defciencies in gastrulation, so8mite

/ormation, head /ormation, heartbeat and pigmentation. + vehicle control "%.7

DS!' was included in all the tests. +ll the tests were per/ormed in triplicate.