perhitungan alt jpt

8/18/2019 Perhitungan Alt Jpt

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CARA PERHITUNGAN ALT


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10 -1

10 0

10 -2

10 -3


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1. a. Σ KOLONI : 30-300b. HANYA DATA 1 PENGENCERAN : 30-300

ALT : Σ KOLONI × FAKTORPENGENCERAN

Contoh :10 -2 : >30010 -3 : 175

10-

: 1!ALT " 175#10 3

" 1$0#10 3 " 1%$#10 5 CFU


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2. ADA 2 PENGENCERAN DENGANΣ KOLONI : 30-300

2 :

≤ 2 & RATA-RATA>2 & GUNAKAN 'ATA PENGENCERAN (G

PERTA)AContoh :*+, 10 -2 : >300

10 -3 : 22

10-

: 30

SEBELUMNYA NPENGENCERAFAKTOR KOLONITINGGILEBIH NPENGENCERAFAKTOR KOLONI

×Σ

×Σ

34,1210.224

10.303

4

=


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ALT " .*22 #10 3 , / *30#10 ," 2!2000 " 2%!#10 5 CFU

* , 10 -2 : >30010 -3 : 2 010 - : 50

ALT " +t+ 4n 4n64 +n 4 t+8+" 2 0#10 3 " 2% #10 5 CFU

083,22

10.240

10.503

4

=≥


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3. SEMUA DATA PETRIDISH


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&. SPREADER

• Ko;on< =4 4 t< +nt+< =+;


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Contoh :10 -2 : >300

10-3

: 2 510 - : 1 PREA'ERALT " 2 5#10 3

" 2%5#105

CFU


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'. SEMUA PETRI( KOLONI : 0ALT: 1 × FAKTOR PENGENCERAN*TEREN'AH,9

Contoh :10 -2 : 0

10-3

: 010 - : 0ALT " 1#10 2 9 CFU


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CONTOH OAL ALT

I II III I10 -2 : 5 7 230 1 1 2 2

10-3

: 173 3 52 101 13710 - : 1! 0 1 2 2!K : 0 0 1 0 0

H


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I, ALT " 1%7 #10 5 CFU

II, ALT "

" .*230#10 2 ,/*3 #10 3 , " 2$500" 2% # 10 CFU

III, 10 -2 : 1 1 -1 " 1 010 -3 : 52 -1 " 51

10 - : 1 -1 " 0

ALT " 1 0 #10 2 " 1% # 10 CFU

043,1210.230

10.342

3

=<

6842,2210.190

10.512

3

=>


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I ,

ALT " 2 # 10 2 " 2% # 10 CFU

,

ALT " 2 # 10 2 " 2%5 # 10 CFU

139,4210.244

10.1012

3

=>

5020,5210.249

10.132

3

=>


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1# '+ < 4n+n+8+n 0%1 8; =+8 4; +<< 4 o;4h +t+ o;on< =4 + +<4 < ?t :

10 -1 : 20/2 = 4+ 4 51310 -2 : 105 35110 -3 : 37 !710 - : 12 2 /1 = 4+ 410 -5 : 3 K : 2H


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2# P+ + 4n+n+8+n 0%5 8; =+8 4;%< 4 o;4h +t+ =4 + +< 4 < ?t :

10 0 : 50 30010 -1 : 101 $010 -2 : 3110 -3 : 5 2 K : 2

H


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+ + +n

1# ? ;# 1 ? ;#2 +t+2 *- K,

10 -1 : 22 513 !7%5 M 3 " ! %510 -2 : 105 351 22$ M 3 " 22510 -3 : 37 !7 52 M 3 "

10 - : 12 30 21 M 3 " 1$10 -5 : 3 3%5 M 3" 0%5

ALT " 225 # 10 2 " 2%3 # 10 B 0%1 8;" 2%3 # 10 5 CFUB 8;

1 8,2210.225

10.49 2

3

=>


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2# ? ;# 1 ? ;#2 +t+2 *- K,10 0 : 50 300 375 M 3 " 37210 -1 : 101 $0 0%5 M 3 " $7%510 -2 : 31 17%5 M 3 " 1 %5

10 -3 : 5 2 3%5 M 3 " 0%5

ALT " $7%5 # 10 1 " $%$ # 10 2 B 0%5 8;" 17%! # 10 2 CFUB 8;

" 1%$ # 103

CFUB 8;


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CARA PERHITUNGAN PT


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1# L


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2#


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"

" 1!%! PTB100 8;*PE) ULATAN 1 ANGKA ELAKANG

KO)A,Contoh 10 1 0%1 8;P4n4 +=+n 0B1 1B3 1B3

PTB100 8; "

" " 15%7


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3# 4 < 4n 4n64 +n 4=


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# 3 =4 < 4n 4n64 +n 4=


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Contoh

1 0%1 0%01 0%001 0%0001 +83B3 2B3 0B3 0B3 0B33+3 2+3 0+3

PT " %3B +8 *t+ 4; 3,

1 0%1 0%01 0%001 0%0001 +8

3B3 3B3 2B3 1B3 1B33+3 2+3 2+3

PT " *t+ 4; 2,


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Contoh1 0%1 0%01 0%001 0%0001 +83B3 2B3 0B3 1B3 0B33+3 2+3 1+3

PT " 15B +8 *t+ 4; 3,

1 0%1 0%01 0%001 8;

0B3 1B3 0B3 0B30+3 1+3 0+3


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Contoh o+;

10 1 0%1 8; T# P4 < ++n 3B3 3B3 3B3

T# P4n4 +=+n 3B3 3B3 2B3 T# P4;4n + 3B3 3B3 2B3H


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1 0%1 0%01 0%001 +80B3 1B3 0B3 0B3H


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• Ko8


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5 0%5 0%05 0%005 0%0005 +8B5 5B5 0B5 1B5 0B5

H

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