PERBEDAAN KADAR GULA DARAH ANTARA SAMPEL SERUM, PLASMA

NaF DAN PLASMA EDTA

Fikri Noor1, Budi Santosa

2, Andri Sukeksi

2

1. Program Studi D IV Analis Kesehatan Fakultas Ilmu Keperawatan dan Kesehatan

Universitas Muhammadiyah Semarang.

2. Laboratorium Patologi Klinik Fakultas Ilmu Keperawatan dan Kesehatan Universitas

Muhammadiyah Semarang.

ABSTRAK

Kadar glukosa darah dapat diperiksa dari sampel darah lengkap (whole blood) yang

berasal dari pembuluh darah kapiler atau vena, serum, dan plasma dengan antikoagulan

Natrium Fluorida (NaF), Na-oxalate, Na-sitrat, atauLithium-heparin. Natrium Fluorida

(NaF) adalah antikoagulan yang sering digunakan untuk sampling bahan pemeriksaan

kadar glukosa darah, karena NaF dianggap mampu menghambat proses glikolisis.

Pemeriksaan kadar glukosa darah umumnya menggunakan sampel serum karena

dilakukan bersamaan dengan pemeriksaan lainnya, sehingga lebih praktis dan efisien.

Tujuan penelitian untuk mengetahui perbedaan kadar gula darah sampel serum, plasma

NaF, dan plasma K2EDTA. Jenis penelitian analitik cross sectional. Hasil penelitian

rerata kadar gula darah serum 174 mg/dl, kadar gula darah NaF rerata 177,15 mg/dl,

kadar gula darah plasma EDTA rerata 74,10 mg/dl. Hasil uji statistik dengan Kruskal

Wallis diperoleh p>0,05 yang berarti tidak terdapat perbedaan yang bermakna pada

kadar gula darah serum, plasma NaF, plasma EDTA. Hal ini dikarenakan waktu

pemeriksaan dilakukan segera setelah pengambilan sampel (10 – 15 menit) sehingga

belum terjadi proses glikolisis yang akan mempengaruhi kadar gula darah.

Kata kunci : kadar gula darah, serum, plasma, EDTA, NaF

repository.unimus.ac.id

DIFFERENTIAL OF BLOOD SUGAR LEVEL BETWEEN SERUM,

NaF PLASMA AND EDTA PLASMA SAMPLE

Fikri Noor1, Budi Santosa

2, Andri Sukeksi

2

1.Study Program of D IV Health Analyst Faculty of Nursing and Health Sciences

University of Muhammadiyah Semarang.

2. Laboratory of Clinical Pathology Faculty of Nursing and Health Sciences University of

Muhammadiyah Semarang.

ABSTRACT

Blood glucose level can be checked from whole blood sample which is from capillary or

venous, serum, and plasma blood vessel with anticoagulant of Sodium Fluoride (NaF),

Na-oxalate, Na-citrate, or Lithium-heparin. Sodium Fluoride (NaF) is an anticoagulant

which is often used for examination material sampling of blood glucose level, because

NaF is considered to be capable in obstructing the glycolysis process. The examination of

blood glucose level is generally using serum samples because it is done simultaneously

with other test, so that more practical and efficient. The research goal is to know the

comparison of blood sugar level of serum, NaF plasma, and K2EDTA plasma sample.

The research type is analytic with cross sectional. The research result of serum blood

sugar level average was 174 mg / dl, NaF blood sugar level average was 177.15 mg / dl,

EDTA plasma blood sugar level average was 74.10 mg / dl. The statistic test result with

Kruskal Wallis was obtained p> 0,05 that means there was no significant difference on

blood sugar level of serum, NaF plasma, EDTA plasma. This is because the timing

checking is done right after the sample taking (about 10 – 15 minute) so this process of

glicolysis which influence the blood sugar level.

Keywords: blood sugar level, serum, plasma, EDTA, NaF

repository.unimus.ac.id

repository.unimus.ac.id