Lampiran 1. Contoh visualisasi hasil pemotongan layang biru

Contoh visualisasi hasil pemotongan sekuen mtDNA biru (D.macarellus) oleh enzim Alu I dan Hind III

Enzim Alu I

Enzim Hind III

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mtDNA D-loop ikan Alu I dan Hind III

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Lampiran 2. Contoh visualisasi hasil pemotongan layang biru (D.Contoh visualisasi hasil pemotongan sekuen mtDNA D-loop ikan

D.macarellus) oleh enzim Mbo I dan Rsa I

Enzim Mbo I

Enzim Rsa I

loop ikan

Lampiran 3. Contoh visualisasi hasil pemotongan layang biru

Contoh visualisasi hasil pemotongan sekuen mtDNA biru (D.macarellus) oleh enzim Taq I dan Xba I

Enzim Taq I

Enzim Xba I

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mtDNA D-loop ikan Taq I dan Xba I

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Lampiran 4. Hasil penghitungan keragaman genetika dengan TFPGA Data set contains genotypes of individuals sampled from populations. Organism Type: Diploid Marker Type: Codominant DESCRIPTIVE STATISTICS ******************************************************** RESULTS FOR EACH POPULATION. POPULATION 1 Locus 1 # obs. at locus= 8 allele: # obs: allele freq: # hets: het freq: 1 8 0.5000 0.0000 0.0000 2 8 0.5000 0.0000 0.0000 Heterozygosity: 0.5000 Heterozygosity (unbiased): 0.5333 Heterozygosity (direct count): 0.0000 Locus 2 # obs. at locus= 8 allele: # obs: allele freq: # hets: het freq: 1 16 1.0000 0.0000 0.0000 Heterozygosity: 0.0000 Heterozygosity (unbiased): 0.0000 Heterozygosity (direct count): 0.0000 Locus 3 # obs. at locus= 8 allele: # obs: allele freq: # hets: het freq: 1 12 0.7500 0.0000 0.0000 2 4 0.2500 0.0000 0.0000 Heterozygosity: 0.3750 Heterozygosity (unbiased): 0.4000 Heterozygosity (direct count): 0.0000 Locus 4 # obs. at locus= 8 allele: # obs: allele freq: # hets: het freq: 1 8 0.5000 0.0000 0.0000 2 4 0.2500 0.0000 0.0000 3 4 0.2500 0.0000 0.0000 Heterozygosity: 0.6250 Heterozygosity (unbiased): 0.6667 Heterozygosity (direct count): 0.0000 Locus 5 # obs. at locus= 8

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allele: # obs: allele freq: # hets: het freq: 1 14 0.8750 0.0000 0.0000 2 0 0.0000 0.0000 0.0000 3 2 0.1250 0.0000 0.0000 Heterozygosity: 0.2188 Heterozygosity (unbiased): 0.2333 Heterozygosity (direct count): 0.0000 Locus 6 # obs. at locus= 8 allele: # obs: allele freq: # hets: het freq: 1 8 0.5000 0.0000 0.0000 2 0 0.0000 0.0000 0.0000 3 8 0.5000 0.0000 0.0000 Heterozygosity: 0.5000 Heterozygosity (unbiased): 0.5333 Heterozygosity (direct count): 0.0000 -------------------------------------------------------- Results over all loci Ave. sample size: 8.0000 Ave. heterozygosity: 0.3698 Ave. heterozygosity (unbiased): 0.3944 Ave. heterozygosity (direct count): 0.0000 % polymorphic loci (no criterion): 83.3333 % polymorphic loci (99% criterion): 83.3333 % polymorphic loci (95% criterion): 83.3333 -------------------------------------------------------- POPULATION 2 Locus 1 # obs. at locus= 10 allele: # obs: allele freq: # hets: het freq: 1 20 1.0000 0.0000 0.0000 2 0 0.0000 0.0000 0.0000 Heterozygosity: 0.0000 Heterozygosity (unbiased): 0.0000 Heterozygosity (direct count): 0.0000 Locus 2 # obs. at locus= 10 allele: # obs: allele freq: # hets: het freq: 1 20 1.0000 0.0000 0.0000 Heterozygosity: 0.0000 Heterozygosity (unbiased): 0.0000 Heterozygosity (direct count): 0.0000

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Locus 3 # obs. at locus= 10 allele: # obs: allele freq: # hets: het freq: 1 20 1.0000 0.0000 0.0000 2 0 0.0000 0.0000 0.0000 Heterozygosity: 0.0000 Heterozygosity (unbiased): 0.0000 Heterozygosity (direct count): 0.0000 Locus 4 # obs. at locus= 10 allele: # obs: allele freq: # hets: het freq: 1 20 1.0000 0.0000 0.0000 2 0 0.0000 0.0000 0.0000 3 0 0.0000 0.0000 0.0000 Heterozygosity: 0.0000 Heterozygosity (unbiased): 0.0000 Heterozygosity (direct count): 0.0000 Locus 5 # obs. at locus= 10 allele: # obs: allele freq: # hets: het freq: 1 14 0.7000 0.0000 0.0000 2 6 0.3000 0.0000 0.0000 3 0 0.0000 0.0000 0.0000 Heterozygosity: 0.4200 Heterozygosity (unbiased): 0.4421 Heterozygosity (direct count): 0.0000 Locus 6 # obs. at locus= 10 allele: # obs: allele freq: # hets: het freq: 1 20 1.0000 0.0000 0.0000 2 0 0.0000 0.0000 0.0000 3 0 0.0000 0.0000 0.0000 Heterozygosity: 0.0000 Heterozygosity (unbiased): 0.0000 Heterozygosity (direct count): 0.0000 -------------------------------------------------------- Results over all loci Ave. sample size: 10.0000 Ave. heterozygosity: 0.0700 Ave. heterozygosity (unbiased): 0.0737 Ave. heterozygosity (direct count): 0.0000 % polymorphic loci (no criterion): 16.6667 % polymorphic loci (99% criterion): 16.6667 % polymorphic loci (95% criterion): 16.6667 --------------------------------------------------------

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POPULATION 3 Locus 1 # obs. at locus= 10 allele: # obs: allele freq: # hets: het freq: 1 20 1.0000 0.0000 0.0000 2 0 0.0000 0.0000 0.0000 Heterozygosity: 0.0000 Heterozygosity (unbiased): 0.0000 Heterozygosity (direct count): 0.0000 Locus 2 # obs. at locus= 10 allele: # obs: allele freq: # hets: het freq: 1 20 1.0000 0.0000 0.0000 Heterozygosity: 0.0000 Heterozygosity (unbiased): 0.0000 Heterozygosity (direct count): 0.0000 Locus 3 # obs. at locus= 10 allele: # obs: allele freq: # hets: het freq: 1 20 1.0000 0.0000 0.0000 2 0 0.0000 0.0000 0.0000 Heterozygosity: 0.0000 Heterozygosity (unbiased): 0.0000 Heterozygosity (direct count): 0.0000 Locus 4 # obs. at locus= 10 allele: # obs: allele freq: # hets: het freq: 1 20 1.0000 0.0000 0.0000 2 0 0.0000 0.0000 0.0000 3 0 0.0000 0.0000 0.0000 Heterozygosity: 0.0000 Heterozygosity (unbiased): 0.0000 Heterozygosity (direct count): 0.0000 Locus 5 # obs. at locus= 10 allele: # obs: allele freq: # hets: het freq: 1 20 1.0000 0.0000 0.0000 2 0 0.0000 0.0000 0.0000 3 0 0.0000 0.0000 0.0000 Heterozygosity: 0.0000 Heterozygosity (unbiased): 0.0000 Heterozygosity (direct count): 0.0000 Locus 6 # obs. at locus= 10 allele: # obs: allele freq: # hets: het freq: 1 20 1.0000 0.0000 0.0000

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2 0 0.0000 0.0000 0.0000 3 0 0.0000 0.0000 0.0000 Heterozygosity: 0.0000 Heterozygosity (unbiased): 0.0000 Heterozygosity (direct count): 0.0000 -------------------------------------------------------- Results over all loci Ave. sample size: 10.0000 Ave. heterozygosity: 0.0000 Ave. heterozygosity (unbiased): 0.0000 Ave. heterozygosity (direct count): 0.0000 % polymorphic loci (no criterion): 0.0000 % polymorphic loci (99% criterion): 0.0000 % polymorphic loci (95% criterion): 0.0000 -------------------------------------------------------- POPULATION 4 Locus 1 # obs. at locus= 10 allele: # obs: allele freq: # hets: het freq: 1 20 1.0000 0.0000 0.0000 2 0 0.0000 0.0000 0.0000 Heterozygosity: 0.0000 Heterozygosity (unbiased): 0.0000 Heterozygosity (direct count): 0.0000 Locus 2 # obs. at locus= 10 allele: # obs: allele freq: # hets: het freq: 1 20 1.0000 0.0000 0.0000 Heterozygosity: 0.0000 Heterozygosity (unbiased): 0.0000 Heterozygosity (direct count): 0.0000 Locus 3 # obs. at locus= 10 allele: # obs: allele freq: # hets: het freq: 1 20 1.0000 0.0000 0.0000 2 0 0.0000 0.0000 0.0000 Heterozygosity: 0.0000 Heterozygosity (unbiased): 0.0000 Heterozygosity (direct count): 0.0000 Locus 4 # obs. at locus= 10 allele: # obs: allele freq: # hets: het freq: 1 20 1.0000 0.0000 0.0000

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2 0 0.0000 0.0000 0.0000 3 0 0.0000 0.0000 0.0000 Heterozygosity: 0.0000 Heterozygosity (unbiased): 0.0000 Heterozygosity (direct count): 0.0000 Locus 5 # obs. at locus= 10 allele: # obs: allele freq: # hets: het freq: 1 20 1.0000 0.0000 0.0000 2 0 0.0000 0.0000 0.0000 3 0 0.0000 0.0000 0.0000 Heterozygosity: 0.0000 Heterozygosity (unbiased): 0.0000 Heterozygosity (direct count): 0.0000 Locus 6 # obs. at locus= 10 allele: # obs: allele freq: # hets: het freq: 1 20 1.0000 0.0000 0.0000 2 0 0.0000 0.0000 0.0000 3 0 0.0000 0.0000 0.0000 Heterozygosity: 0.0000 Heterozygosity (unbiased): 0.0000 Heterozygosity (direct count): 0.0000 -------------------------------------------------------- Results over all loci Ave. sample size: 10.0000 Ave. heterozygosity: 0.0000 Ave. heterozygosity (unbiased): 0.0000 Ave. heterozygosity (direct count): 0.0000 % polymorphic loci (no criterion): 0.0000 % polymorphic loci (99% criterion): 0.0000 % polymorphic loci (95% criterion): 0.0000 -------------------------------------------------------- POPULATION 5 Locus 1 # obs. at locus= 9 allele: # obs: allele freq: # hets: het freq: 1 18 1.0000 0.0000 0.0000 2 0 0.0000 0.0000 0.0000 Heterozygosity: 0.0000 Heterozygosity (unbiased): 0.0000 Heterozygosity (direct count): 0.0000

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Locus 2 # obs. at locus= 9 allele: # obs: allele freq: # hets: het freq: 1 18 1.0000 0.0000 0.0000 Heterozygosity: 0.0000 Heterozygosity (unbiased): 0.0000 Heterozygosity (direct count): 0.0000 Locus 3 # obs. at locus= 9 allele: # obs: allele freq: # hets: het freq: 1 18 1.0000 0.0000 0.0000 2 0 0.0000 0.0000 0.0000 Heterozygosity: 0.0000 Heterozygosity (unbiased): 0.0000 Heterozygosity (direct count): 0.0000 Locus 4 # obs. at locus= 9 allele: # obs: allele freq: # hets: het freq: 1 18 1.0000 0.0000 0.0000 2 0 0.0000 0.0000 0.0000 3 0 0.0000 0.0000 0.0000 Heterozygosity: 0.0000 Heterozygosity (unbiased): 0.0000 Heterozygosity (direct count): 0.0000 Locus 5 # obs. at locus= 9 allele: # obs: allele freq: # hets: het freq: 1 18 1.0000 0.0000 0.0000 2 0 0.0000 0.0000 0.0000 3 0 0.0000 0.0000 0.0000 Heterozygosity: 0.0000 Heterozygosity (unbiased): 0.0000 Heterozygosity (direct count): 0.0000 Locus 6 # obs. at locus= 9 allele: # obs: allele freq: # hets: het freq: 1 18 1.0000 0.0000 0.0000 2 0 0.0000 0.0000 0.0000 3 0 0.0000 0.0000 0.0000 Heterozygosity: 0.0000 Heterozygosity (unbiased): 0.0000 Heterozygosity (direct count): 0.0000 -------------------------------------------------------- Results over all loci Ave. sample size: 9.0000

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Ave. heterozygosity: 0.0000 Ave. heterozygosity (unbiased): 0.0000 Ave. heterozygosity (direct count): 0.0000 % polymorphic loci (no criterion): 0.0000 % polymorphic loci (99% criterion): 0.0000 % polymorphic loci (95% criterion): 0.0000 -------------------------------------------------------- POPULATION 6 Locus 1 # obs. at locus= 8 allele: # obs: allele freq: # hets: het freq: 1 16 1.0000 0.0000 0.0000 2 0 0.0000 0.0000 0.0000 Heterozygosity: 0.0000 Heterozygosity (unbiased): 0.0000 Heterozygosity (direct count): 0.0000 Locus 2 # obs. at locus= 8 allele: # obs: allele freq: # hets: het freq: 1 16 1.0000 0.0000 0.0000 Heterozygosity: 0.0000 Heterozygosity (unbiased): 0.0000 Heterozygosity (direct count): 0.0000 Locus 3 # obs. at locus= 8 allele: # obs: allele freq: # hets: het freq: 1 16 1.0000 0.0000 0.0000 2 0 0.0000 0.0000 0.0000 Heterozygosity: 0.0000 Heterozygosity (unbiased): 0.0000 Heterozygosity (direct count): 0.0000 Locus 4 # obs. at locus= 8 allele: # obs: allele freq: # hets: het freq: 1 16 1.0000 0.0000 0.0000 2 0 0.0000 0.0000 0.0000 3 0 0.0000 0.0000 0.0000 Heterozygosity: 0.0000 Heterozygosity (unbiased): 0.0000 Heterozygosity (direct count): 0.0000 Locus 5 # obs. at locus= 8 allele: # obs: allele freq: # hets: het freq: 1 16 1.0000 0.0000 0.0000 2 0 0.0000 0.0000 0.0000

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3 0 0.0000 0.0000 0.0000 Heterozygosity: 0.0000 Heterozygosity (unbiased): 0.0000 Heterozygosity (direct count): 0.0000 Locus 6 # obs. at locus= 8 allele: # obs: allele freq: # hets: het freq: 1 16 1.0000 0.0000 0.0000 2 0 0.0000 0.0000 0.0000 3 0 0.0000 0.0000 0.0000 Heterozygosity: 0.0000 Heterozygosity (unbiased): 0.0000 Heterozygosity (direct count): 0.0000 -------------------------------------------------------- Results over all loci Ave. sample size: 8.0000 Ave. heterozygosity: 0.0000 Ave. heterozygosity (unbiased): 0.0000 Ave. heterozygosity (direct count): 0.0000 % polymorphic loci (no criterion): 0.0000 % polymorphic loci (99% criterion): 0.0000 % polymorphic loci (95% criterion): 0.0000 -------------------------------------------------------- POPULATION 7 Locus 1 # obs. at locus= 8 allele: # obs: allele freq: # hets: het freq: 1 16 1.0000 0.0000 0.0000 2 0 0.0000 0.0000 0.0000 Heterozygosity: 0.0000 Heterozygosity (unbiased): 0.0000 Heterozygosity (direct count): 0.0000 Locus 2 # obs. at locus= 8 allele: # obs: allele freq: # hets: het freq: 1 16 1.0000 0.0000 0.0000 Heterozygosity: 0.0000 Heterozygosity (unbiased): 0.0000 Heterozygosity (direct count): 0.0000 Locus 3 # obs. at locus= 8 allele: # obs: allele freq: # hets: het freq: 1 16 1.0000 0.0000 0.0000

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2 0 0.0000 0.0000 0.0000 Heterozygosity: 0.0000 Heterozygosity (unbiased): 0.0000 Heterozygosity (direct count): 0.0000 Locus 4 # obs. at locus= 8 allele: # obs: allele freq: # hets: het freq: 1 16 1.0000 0.0000 0.0000 2 0 0.0000 0.0000 0.0000 3 0 0.0000 0.0000 0.0000 Heterozygosity: 0.0000 Heterozygosity (unbiased): 0.0000 Heterozygosity (direct count): 0.0000 Locus 5 # obs. at locus= 8 allele: # obs: allele freq: # hets: het freq: 1 16 1.0000 0.0000 0.0000 2 0 0.0000 0.0000 0.0000 3 0 0.0000 0.0000 0.0000 Heterozygosity: 0.0000 Heterozygosity (unbiased): 0.0000 Heterozygosity (direct count): 0.0000 Locus 6 # obs. at locus= 8 allele: # obs: allele freq: # hets: het freq: 1 16 1.0000 0.0000 0.0000 2 0 0.0000 0.0000 0.0000 3 0 0.0000 0.0000 0.0000 Heterozygosity: 0.0000 Heterozygosity (unbiased): 0.0000 Heterozygosity (direct count): 0.0000 -------------------------------------------------------- Results over all loci Ave. sample size: 8.0000 Ave. heterozygosity: 0.0000 Ave. heterozygosity (unbiased): 0.0000 Ave. heterozygosity (direct count): 0.0000 % polymorphic loci (no criterion): 0.0000 % polymorphic loci (99% criterion): 0.0000 % polymorphic loci (95% criterion): 0.0000 -------------------------------------------------------- POPULATION 8

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Locus 1 # obs. at locus= 8 allele: # obs: allele freq: # hets: het freq: 1 16 1.0000 0.0000 0.0000 2 0 0.0000 0.0000 0.0000 Heterozygosity: 0.0000 Heterozygosity (unbiased): 0.0000 Heterozygosity (direct count): 0.0000 Locus 2 # obs. at locus= 8 allele: # obs: allele freq: # hets: het freq: 1 16 1.0000 0.0000 0.0000 Heterozygosity: 0.0000 Heterozygosity (unbiased): 0.0000 Heterozygosity (direct count): 0.0000 Locus 3 # obs. at locus= 8 allele: # obs: allele freq: # hets: het freq: 1 16 1.0000 0.0000 0.0000 2 0 0.0000 0.0000 0.0000 Heterozygosity: 0.0000 Heterozygosity (unbiased): 0.0000 Heterozygosity (direct count): 0.0000 Locus 4 # obs. at locus= 8 allele: # obs: allele freq: # hets: het freq: 1 16 1.0000 0.0000 0.0000 2 0 0.0000 0.0000 0.0000 3 0 0.0000 0.0000 0.0000 Heterozygosity: 0.0000 Heterozygosity (unbiased): 0.0000 Heterozygosity (direct count): 0.0000 Locus 5 # obs. at locus= 8 allele: # obs: allele freq: # hets: het freq: 1 16 1.0000 0.0000 0.0000 2 0 0.0000 0.0000 0.0000 3 0 0.0000 0.0000 0.0000 Heterozygosity: 0.0000 Heterozygosity (unbiased): 0.0000 Heterozygosity (direct count): 0.0000 Locus 6 # obs. at locus= 8 allele: # obs: allele freq: # hets: het freq: 1 14 0.8750 0.0000 0.0000 2 2 0.1250 0.0000 0.0000 3 0 0.0000 0.0000 0.0000

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Heterozygosity: 0.2188 Heterozygosity (unbiased): 0.2333 Heterozygosity (direct count): 0.0000 -------------------------------------------------------- Results over all loci Ave. sample size: 8.0000 Ave. heterozygosity: 0.0365 Ave. heterozygosity (unbiased): 0.0389 Ave. heterozygosity (direct count): 0.0000 % polymorphic loci (no criterion): 16.6667 % polymorphic loci (99% criterion): 16.6667 % polymorphic loci (95% criterion): 16.6667

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Lampiran 5. Hasil penghitungan Analisis Berpasangan Fst Data set contains genotypes of individuals sampled from populations. Organism Type: Diploid Marker Type: Codominant Exact tests for population differentiation (Raymond and Rousset 1995) # of dememorization steps: 1000 # of batches: 10 # of permutations per batch: 2000 Pairwise analysis of all populations Groups compared: 1 vs. 2 locus 1 : p = 0.0004 S.E. :0.0002 Locus 2 :Only one allele at locus. Analysis not performed. locus 3 : p = 0.0301 S.E. :0.0019 locus 4 : p = 0.0003 S.E. :0.0001 locus 5 : p = 0.0118 S.E. :0.0015 locus 6 : p = 0.0003 S.E. :0.0002 Results over loci X-sq : 64.6125 df : 10 Overall: p = 0.0000 Groups compared: 1 vs. 3 locus 1 : p = 0.0006 S.E. :0.0002 Locus 2 :Only one allele at locus. Analysis not performed. locus 3 : p = 0.0295 S.E. :0.0019 locus 4 : p = 0.0004 S.E. :0.0004 locus 5 : p = 0.1972 S.E. :0.0040 locus 6 : p = 0.0004 S.E. :0.0002 Results over loci X-sq : 56.9652 df : 10 Overall: p = 0.0000 Groups compared: 1 vs. 4 locus 1 : p = 0.0006 S.E. :0.0004 Locus 2 :Only one allele at locus. Analysis not performed.

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locus 3 : p = 0.0386 S.E. :0.0029 locus 4 : p = 0.0000 S.E. :0.0000 locus 5 : p = 0.1927 S.E. :0.0024 locus 6 : p = 0.0008 S.E. :0.0005 Results over loci X-sq : 59.0113 df : 10 Overall: p = 0.0000 Groups compared: 1 vs. 5 locus 1 : p = 0.0006 S.E. :0.0002 Locus 2 :Only one allele at locus. Analysis not performed. locus 3 : p = 0.0382 S.E. :0.0021 locus 4 : p = 0.0002 S.E. :0.0002 locus 5 : p = 0.2121 S.E. :0.0030 locus 6 : p = 0.0008 S.E. :0.0005 Results over loci X-sq : 55.9412 df : 10 Overall: p = 0.0000 Groups compared: 1 vs. 6 locus 1 : p = 0.0018 S.E. :0.0005 Locus 2 :Only one allele at locus. Analysis not performed. locus 3 : p = 0.0950 S.E. :0.0043 locus 4 : p = 0.0023 S.E. :0.0006 locus 5 : p = 0.4882 S.E. :0.0030 locus 6 : p = 0.0024 S.E. :0.0005 Results over loci X-sq : 43.1397 df : 10 Overall: p = 0.0000 Groups compared: 1 vs. 7 locus 1 : p = 0.0022 S.E. :0.0007 Locus 2 :Only one allele at locus. Analysis not performed. locus 3 : p = 0.1011 S.E. :0.0033

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locus 4 : p = 0.0035 S.E. :0.0008 locus 5 : p = 0.4865 S.E. :0.0035 locus 6 : p = 0.0025 S.E. :0.0007 Results over loci X-sq : 41.6710 df : 10 Overall: p = 0.0000 Groups compared: 1 vs. 8 locus 1 : p = 0.0016 S.E. :0.0005 Locus 2 :Only one allele at locus. Analysis not performed. locus 3 : p = 0.1040 S.E. :0.0040 locus 4 : p = 0.0016 S.E. :0.0006 locus 5 : p = 0.4837 S.E. :0.0049 locus 6 : p = 0.0011 S.E. :0.0003 Results over loci X-sq : 45.5117 df : 10 Overall: p = 0.0000 Groups compared: 2 vs. 3 locus 1 : p = 1.0000 S.E. :0.0000 Locus 2 :Only one allele at locus. Analysis not performed. locus 3 : p = 1.0000 S.E. :0.0000 locus 4 : p = 1.0000 S.E. :0.0000 locus 5 : p = 0.0217 S.E. :0.0009 locus 6 : p = 1.0000 S.E. :0.0000 Results over loci X-sq : 7.6609 df : 10 Overall: p = 0.6619 Groups compared: 2 vs. 4 locus 1 : p = 1.0000 S.E. :0.0000 Locus 2 :Only one allele at locus. Analysis not performed. locus 3 : p = 1.0000 S.E. :0.0000 locus 4 : p = 1.0000 S.E. :0.0000

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locus 5 : p = 0.0179 S.E. :0.0013 locus 6 : p = 1.0000 S.E. :0.0000 Results over loci X-sq : 8.0515 df : 10 Overall: p = 0.6238 Groups compared: 2 vs. 5 locus 1 : p = 1.0000 S.E. :0.0000 Locus 2 :Only one allele at locus. Analysis not performed. locus 3 : p = 1.0000 S.E. :0.0000 locus 4 : p = 1.0000 S.E. :0.0000 locus 5 : p = 0.0177 S.E. :0.0021 locus 6 : p = 1.0000 S.E. :0.0000 Results over loci X-sq : 8.0684 df : 10 Overall: p = 0.6222 Groups compared: 2 vs. 6 locus 1 : p = 1.0000 S.E. :0.0000 Locus 2 :Only one allele at locus. Analysis not performed. locus 3 : p = 1.0000 S.E. :0.0000 locus 4 : p = 1.0000 S.E. :0.0000 locus 5 : p = 0.0273 S.E. :0.0021 locus 6 : p = 1.0000 S.E. :0.0000 Results over loci X-sq : 7.2017 df : 10 Overall: p = 0.7063 Groups compared: 2 vs. 7 locus 1 : p = 1.0000 S.E. :0.0000 Locus 2 :Only one allele at locus. Analysis not performed. locus 3 : p = 1.0000 S.E. :0.0000 locus 4 : p = 1.0000 S.E. :0.0000 locus 5 : p = 0.0205 S.E. :0.0014

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locus 6 : p = 1.0000 S.E. :0.0000 Results over loci X-sq : 7.7747 df : 10 Overall: p = 0.6508 Groups compared: 2 vs. 8 locus 1 : p = 1.0000 S.E. :0.0000 Locus 2 :Only one allele at locus. Analysis not performed. locus 3 : p = 1.0000 S.E. :0.0000 locus 4 : p = 1.0000 S.E. :0.0000 locus 5 : p = 0.0256 S.E. :0.0031 locus 6 : p = 0.1848 S.E. :0.0043 Results over loci X-sq : 10.7073 df : 10 Overall: p = 0.3808 Groups compared: 3 vs. 4 locus 1 : p = 1.0000 S.E. :0.0000 Locus 2 :Only one allele at locus. Analysis not performed. locus 3 : p = 1.0000 S.E. :0.0000 locus 4 : p = 1.0000 S.E. :0.0000 locus 5 : p = 1.0000 S.E. :0.0000 locus 6 : p = 1.0000 S.E. :0.0000 Results over loci X-sq : 0.0000 df : 10 Overall: p = 1.0000 Groups compared: 3 vs. 5 locus 1 : p = 1.0000 S.E. :0.0000 Locus 2 :Only one allele at locus. Analysis not performed. locus 3 : p = 1.0000 S.E. :0.0000 locus 4 : p = 1.0000 S.E. :0.0000 locus 5 : p = 1.0000 S.E. :0.0000 locus 6 : p = 1.0000 S.E. :0.0000

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Results over loci X-sq : 0.0000 df : 10 Overall: p = 1.0000 Groups compared: 3 vs. 6 locus 1 : p = 1.0000 S.E. :0.0000 Locus 2 :Only one allele at locus. Analysis not performed. locus 3 : p = 1.0000 S.E. :0.0000 locus 4 : p = 1.0000 S.E. :0.0000 locus 5 : p = 1.0000 S.E. :0.0000 locus 6 : p = 1.0000 S.E. :0.0000 Results over loci X-sq : 0.0000 df : 10 Overall: p = 1.0000 Groups compared: 3 vs. 7 locus 1 : p = 1.0000 S.E. :0.0000 Locus 2 :Only one allele at locus. Analysis not performed. locus 3 : p = 1.0000 S.E. :0.0000 locus 4 : p = 1.0000 S.E. :0.0000 locus 5 : p = 1.0000 S.E. :0.0000 locus 6 : p = 1.0000 S.E. :0.0000 Results over loci X-sq : 0.0000 df : 10 Overall: p = 1.0000 Groups compared: 3 vs. 8 locus 1 : p = 1.0000 S.E. :0.0000 Locus 2 :Only one allele at locus. Analysis not performed. locus 3 : p = 1.0000 S.E. :0.0000 locus 4 : p = 1.0000 S.E. :0.0000 locus 5 : p = 1.0000 S.E. :0.0000 locus 6 : p = 0.1867 S.E. :0.0040

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Results over loci X-sq : 3.3565 df : 10 Overall: p = 0.9717 Groups compared: 4 vs. 5 locus 1 : p = 1.0000 S.E. :0.0000 Locus 2 :Only one allele at locus. Analysis not performed. locus 3 : p = 1.0000 S.E. :0.0000 locus 4 : p = 1.0000 S.E. :0.0000 locus 5 : p = 1.0000 S.E. :0.0000 locus 6 : p = 1.0000 S.E. :0.0000 Results over loci X-sq : 0.0000 df : 10 Overall: p = 1.0000 Groups compared: 4 vs. 6 locus 1 : p = 1.0000 S.E. :0.0000 Locus 2 :Only one allele at locus. Analysis not performed. locus 3 : p = 1.0000 S.E. :0.0000 locus 4 : p = 1.0000 S.E. :0.0000 locus 5 : p = 1.0000 S.E. :0.0000 locus 6 : p = 1.0000 S.E. :0.0000 Results over loci X-sq : 0.0000 df : 10 Overall: p = 1.0000 Groups compared: 4 vs. 7 locus 1 : p = 1.0000 S.E. :0.0000 Locus 2 :Only one allele at locus. Analysis not performed. locus 3 : p = 1.0000 S.E. :0.0000 locus 4 : p = 1.0000 S.E. :0.0000 locus 5 : p = 1.0000 S.E. :0.0000 locus 6 : p = 1.0000 S.E. :0.0000 Results over loci

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X-sq : 0.0000 df : 10 Overall: p = 1.0000 Groups compared: 4 vs. 8 locus 1 : p = 1.0000 S.E. :0.0000 Locus 2 :Only one allele at locus. Analysis not performed. locus 3 : p = 1.0000 S.E. :0.0000 locus 4 : p = 1.0000 S.E. :0.0000 locus 5 : p = 1.0000 S.E. :0.0000 locus 6 : p = 0.1862 S.E. :0.0026 Results over loci X-sq : 3.3624 df : 10 Overall: p = 0.9716 Groups compared: 5 vs. 6 locus 1 : p = 1.0000 S.E. :0.0000 Locus 2 :Only one allele at locus. Analysis not performed. locus 3 : p = 1.0000 S.E. :0.0000 locus 4 : p = 1.0000 S.E. :0.0000 locus 5 : p = 1.0000 S.E. :0.0000 locus 6 : p = 1.0000 S.E. :0.0000 Results over loci X-sq : 0.0000 df : 10 Overall: p = 1.0000 Groups compared: 5 vs. 7 locus 1 : p = 1.0000 S.E. :0.0000 Locus 2 :Only one allele at locus. Analysis not performed. locus 3 : p = 1.0000 S.E. :0.0000 locus 4 : p = 1.0000 S.E. :0.0000 locus 5 : p = 1.0000 S.E. :0.0000 locus 6 : p = 1.0000 S.E. :0.0000 Results over loci X-sq : 0.0000

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df : 10 Overall: p = 1.0000 Groups compared: 5 vs. 8 locus 1 : p = 1.0000 S.E. :0.0000 Locus 2 :Only one allele at locus. Analysis not performed. locus 3 : p = 1.0000 S.E. :0.0000 locus 4 : p = 1.0000 S.E. :0.0000 locus 5 : p = 1.0000 S.E. :0.0000 locus 6 : p = 0.2150 S.E. :0.0036 Results over loci X-sq : 3.0747 df : 10 Overall: p = 0.9796 Groups compared: 6 vs. 7 locus 1 : p = 1.0000 S.E. :0.0000 Locus 2 :Only one allele at locus. Analysis not performed. locus 3 : p = 1.0000 S.E. :0.0000 locus 4 : p = 1.0000 S.E. :0.0000 locus 5 : p = 1.0000 S.E. :0.0000 locus 6 : p = 1.0000 S.E. :0.0000 Results over loci X-sq : 0.0000 df : 10 Overall: p = 1.0000 Groups compared: 6 vs. 8 locus 1 : p = 1.0000 S.E. :0.0000 Locus 2 :Only one allele at locus. Analysis not performed. locus 3 : p = 1.0000 S.E. :0.0000 locus 4 : p = 1.0000 S.E. :0.0000 locus 5 : p = 1.0000 S.E. :0.0000 locus 6 : p = 0.4830 S.E. :0.0038 Results over loci X-sq : 1.4557 df : 10

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Overall: p = 0.9991 Groups compared: 7 vs. 8 locus 1 : p = 1.0000 S.E. :0.0000 Locus 2 :Only one allele at locus. Analysis not performed. locus 3 : p = 1.0000 S.E. :0.0000 locus 4 : p = 1.0000 S.E. :0.0000 locus 5 : p = 1.0000 S.E. :0.0000 locus 6 : p = 0.4868 S.E. :0.0029 Results over loci X-sq : 1.4398 df : 10 Overall: p = 0.9991 Matrix of combined probabilities for each pairwise comparison 1 2 3 4 5 6 7 8 1 ***** 2 0.0000 ***** 3 0.0000 0.6619 ***** 4 0.0000 0.6238 1.0000 ***** 5 0.0000 0.6222 1.0000 1.0000 ***** 6 0.0000 0.7063 1.0000 1.0000 1.0000 ***** 7 0.0000 0.6508 1.0000 1.0000 1.0000 1.0000 ***** 8 0.0000 0.3808 0.9717 0.9716 0.9796 0.9991 0.9991 *****

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Lampiran 6. Hasil penghitungan Jarak Genetika dengan TFPGA Data set contains genotypes of individuals sampled from populations. Organism Type: Diploid Marker Type: Codominant GENETIC DISTANCES NEI'S (1972/1978) IDENTITIES/DISTANCES Populations unbiased unbiased compared dist. ident. dist. ident. 1 vs. 2 0.1733 0.8409 0.1514 0.8595 1 vs. 3 0.1438 0.8660 0.1239 0.8835 1 vs. 4 0.1438 0.8660 0.1239 0.8835 1 vs. 5 0.1438 0.8660 0.1239 0.8835 1 vs. 6 0.1438 0.8660 0.1239 0.8835 1 vs. 7 0.1438 0.8660 0.1239 0.8835 1 vs. 8 0.1405 0.8689 0.1193 0.8875 2 vs. 3 0.0150 0.9851 0.0130 0.9871 2 vs. 4 0.0150 0.9851 0.0130 0.9871 2 vs. 5 0.0150 0.9851 0.0130 0.9871 2 vs. 6 0.0150 0.9851 0.0130 0.9871 2 vs. 7 0.0150 0.9851 0.0130 0.9871 2 vs. 8 0.0186 0.9816 0.0154 0.9848 3 vs. 4 0.0000 1.0000 0.0000 1.0000 3 vs. 5 0.0000 1.0000 0.0000 1.0000 3 vs. 6 0.0000 1.0000 0.0000 1.0000 3 vs. 7 0.0000 1.0000 0.0000 1.0000 3 vs. 8 0.0025 0.9975 0.0012 0.9988 4 vs. 5 0.0000 1.0000 0.0000 1.0000 4 vs. 6 0.0000 1.0000 0.0000 1.0000 4 vs. 7 0.0000 1.0000 0.0000 1.0000 4 vs. 8 0.0025 0.9975 0.0012 0.9988 5 vs. 6 0.0000 1.0000 0.0000 1.0000 5 vs. 7 0.0000 1.0000 0.0000 1.0000 5 vs. 8 0.0025 0.9975 0.0012 0.9988 6 vs. 7 0.0000 1.0000 0.0000 1.0000 6 vs. 8 0.0025 0.9975 0.0012 0.9988 7 vs. 8 0.0025 0.9975 0.0012 0.9988