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LAMPIRAN

TOKSISITAS AKUT EKSTRAK…, FITRI KURNIAWATI, FAKULTAS FARMASI UMP, 2014

55

Lampiran 1.Surat persetujuan etik


56

Lampiran 2.Surat keterangan determinasi


57


58

Lampiran 3. Bagan rancangan dosis pada sighting study

Perhitungan dosis uji pendahuluan

1. Dosis = 300 mg/kg BB

BB hewan uji = 125 gram

= 0,125 kg


59

Diambil 2 mL untuk di suntikan secara peroral pada hewan uji



= 0,130 kg




= 0,145 kg


60

Diambil 2 mL untuk di suntikan secara peroral pada hewan uji, tidak

ditemukan kematian pada hewan uji, sehingga pada hari berikutnya dosis

dinaikan menjadi 2000 mg/kg BB



= 0,145 kg




= 0,125 kg



61



= 0,130 kg

Diambil 2 mL untuk di suntikan secara peroral pada hewan uji, tidak

terjadi kematian, sehingga pada main study digunakan dosis 2000 mg/kg

BB


62

Lampiran 4. Pengamatan Gejala Efek toksik

Dosis 300 mg/kg BB Dosis 2000 mg/kg bb

1 2 3 1 2 3

kegelisahan X X X X X X

keberangasan X X X X X X

agresif X X X X X X

ketakutan X X X X X X

kebingungan X X X X X X

muntah X X X X X X

Diare X X X X X X

tinjaberdarah X X X X X X

Kerontokanbulu X X X X X X

Kulitkemerahan

bengkak

X

X

X

X

X

X

X

X

X

X

X

X

61


63

lampiran 5. Gambar klasifikasi GHS


64

Lampiran 6. Bagan rancangan dosis pada main study

Data perhitungan dosis pada uji utama (main study) pada kelompok

perlakuan



= 0,155 kg



65



= 0,123 kg




= 0,130 kg




= 0,179 kg


66




= 0,160 kg

Diambil 2 mL untuk di suntikan secara peroral pada hewan uji.


67

Lampiran 7. Hasil pengamatan gejala efek toksik selama 14 hari

No h-1 h+1 h+2 h+3 h+4 h+5 h+6 h+7 h+8 h+9 h+10 h+11 h+12 h+13 h+14

1 X X X X X X X X X X X X X X X










Ket: kelompok kontrol : tikus no 6-10, kelompok perlakuan: tikus no 1-5


68

Lampiran 8. Data berat badan (gram) hewan uji selama 14 hari

tikus

No h-1 h+1 h+2 h+3 h+4 h+5 h+6 h+7 h+8 h+9 h+10 h+11 h+12 h+13 h+14

1 160 173 167 182 183 186.5 190 189 185 181 185 192 190 192 195

2 127 180 180 200 204 197 191.5 198 191.5 189 194 200 203 200 190

3 136 197 164 182 195 197 199 184 184 184 188 185 209 189 184

4 183 136 138 155 159 159.5 163 159 161 156 163 165 186 209 200

5 163 187 183 200 209 211.5 213.5 214.5 200 198 206 201 165 189 173

6 126 154 141 147 157 151.5 146 156 149 144 154 154 153 155 149

7 150 196 165 179 183 189 195 188 185 174 194 194 197 195 189

8 127.5 140 160 174.5 173 176 179 180.5 177 168 162.5 155 179 172 182

9 164 195 137 153 156 159 159 156 154 150 159 152 155 150 154

10 130 174.5 161 173 180 182.5 185 179 176.5 174 173 177 185 187.5 182


69

Lampiran 9. Data hasil jumlah eritrosit (mm3)

tikus no h-1 h+1 h+7 h+14

1 7600000 6000000 11200000 6400000

2 8400000 12000000 12800000 8800000

3 8350000 10400000 16000000 7200000

4 9250000 5400000 24000000 8800000

5 6500000 6600000 8800000 5600000

6 5500000 10400000 7500000 4000000

7 9300000 11200000 25000000 6400000

8 8780000 6000000 17600000 9600000

9 7000000 11200000 20000000 4000000

10 9080000 12000000 10200000 8000000


70


1 3000 7850 9600 6400

2 6100 7500 7050 7800

3 6500 9600 7900 8200

4 3600 6400 6750 7650

5 3400 6750 9050 9600

6 4500 9050 9800 9600

7 5500 7650 6950 6800

8 5200 6900 9600 6400

9 5650 7050 7850 7650

10 5950 7900 8800 7950

Lampiran 10. Data hasil jumlah leukosit (mm3)


71

Lampiran 11. Data hasil pengukuran enzim GPT (U/L)


1 69.4 71.1 73.9 123.4

2 66.4 173.3 122.1 102.7

3 71.3 124.8 117.9 101.9

4 102.1 137 97.1 60.5

5 120.5 86.4 125.9 107.8

6 77 107.2 117.7 116.3

7 69 84.7 209.4 79.7

8 77.4 111.6 131.1 110.4

9 41.2 101.1 99.6 92

10 67.6 86.2 141.4 75.5


72

Lampiran 12. Data hasil penguukuran enzim GOT (U/L)


1 64.4 64.9 29.7 28

2 17.1 44.7 34.3 27

3 19.4 38.6 31.2 42.8

4 16.9 21 26.7 21.3

5 48.7 23.4 20.9 25.8

6 18.2 37 43.2 22.2

7 18.1 26.1 54.5 13.4

8 23.12 34.5 31 23

9 22.3 27 15.5 21.3

10 24 24.9 32.1 23.6


73

Lampiran 13. Data hasil pengukuran urea darah (mg/dL)

Hewan uji h-1 h+1 h+7 h+14

1 21 25 35 28

2 48 26 31 26

3 23 38 36 23

4 40 33 29 25

5 52 95 39 36

6 29 35 35 23

7 48 28 29 23

8 31 37 26 31

9 42 33 35 36

10 24 23 32 28


74

Lampiran 14. Data hasil penimbangan bobot organ hati dan ginjal

tikus no hati ginjal

Kanan kiri

1 7.547 0.492 0.502

2 7.427 0.616 0.609

3 7.369 0.622 0.602

4 7.762 0.583 0.578

5 6.553 0.555 0.511

6 9.149 0.661 0.646

7 7.676 0.561 0.533

8 6.753 0.518 0.417

9 6.124 0.524 0.518

10 6.932 0.518 0.501


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Lampiran 15. Hasil uji histopatologi


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Lampiran 16. Gambar hasil pengumpulan dan penyiapan ekstrak

Proses pengeringan Daun telah kering

Pengayakan Penimbangan


77

Maserasi

Serbuk kering Penguapan

Pengadukan


78

Ekstrak etanol daun binahong


79

Lampiran 17. Gambar Penyiapan hewan uji

Penyimpanan hewan uji Penyuntikan secara peroral

Pengamatan gejala efek toksik Pengambilan darah


80

Lampiran 18. Gambar penyiapan uji biokimia

Spektrofotometer Layar spektrofotometer

Persiapan uji biokimia Plasma EDTA


81

Lampiran 19. Gambar Pembedahan

Alat bedah

Pembedahan Pengambilan organ

Euthensia


82

Pengawetan organ hati Pengawetan organ ginjal


83

Lampiran 20. Hasil Analisis Statistik

1. Analisis statistik berat badan

a. Uji T berpasangan

Paired Samples Statistics

Mean N Std. Deviation Std. Error Mean

Pair 1 hmin1 153,80 5 22,42 10,02

Minggu1 184,10 5 17,65 7,89

Paired Samples Correlations

N Correlation Sig.

Pair 1 hmin1 & minggu1 5 -0,617 0,268

Paired Samples Test

Paired Differences

t df

Sig. (2-

tailed)

Mean Std. Deviation

Std. Error

Mean

95% Confidence Interval of

the Difference

Lower Upper

Pair 1 hmin1 –

minggu1 -30,30 36,09 16,14 -75,11 14.51 -1.87 4 0,13



Pair 1 hmin1 153,80 5 22,42 10,02

Minggu1 188,11 5 6,73 3,01


N Correlation Sig.

Pair 1 hmin1 & minggu1 5 -0,84 0,06


84

b. Analisis uji T berpasangan

Group Statistics

kelompokuji N Mean Std. Deviation Std. Error Mean

hmin1 Perlakuan 5 153,80 22.42 10.02

nonperlakuan 5 139,50 16.78 7.50

minggu1 Perlakuan 5 184,10 17.65 7.89

nonperlakuan 5 169,89 10.65 4.76

minggu2 Perlakuan 5 188,11 6.73 3.01

nonperlakuan 5 168,85 16.57 7.41

Levene's Test for

Equality of

Variances t-test for Equality of Means

F Sig. T df

Sig. (2-

tailed)

Mean

Difference

Std. Error

Difference

95% Confidence

Interval of the

Difference

Lower Upper

hmin1 Equal variances assumed 0,521 0,49 1,14 8 0,28 14,30 12,52 -14,58 43,18

Equal variances not assumed

1,14 7,4 0,28 14,30 12,52 -14,98 43,58

minggu1 Equal variances assumed 0,59 0,46 1,54 8 0,62 14,21 9,22 -7,05 35,47


1,51 6,57 0,17 14,21 9,22 -7,89 36,31

minggu2 Equal variances assumed 5,17 0,05 2,4 8 0,04 19,26 8,00 0.81 37.71


2,40 5,28 0.05 19,26 8,00 -0.96 39.49


85

2. Hasil analisis statistik sel darah merah

a. Uji T tidak berpasangan jumlah

Group Statistics


hmin1 perlakuan 5 8,0200E6 1,03114E6 4,61140E5

nonperlakuan 5 7,9320E6 1,63491E6 7,31153E5

hplus1 perlakuan 5 8,0800E6 2,93462E6 1,31240E6






Independent Samples Test

Levene's Test

for Equality of


F Sig. t df

Sig. (2-

tailed)

Mean

Differen

ce

Std. Error

Difference

95% Confidence

Interval of the

Difference

Lower Upper

hmin1 Equal variances assumed 2,25 0,17 0,10 8 0,92 8,80E4 8,64E5 -1,91E6 2,08E6


0,10 6,74 0,92 8,80E4 8,64E5 -1,97E6 2,15E6

hplus1 Equal variances assumed 1,12 0,32 -1,22 8 0,25 -2,08E6 1,69E6 -5,98E6 1,82E6


-1,22 7,68 0,25 -2,08E6 1,69E6 -6,01E6 1,82E6

hplus7 Equal variances assumed 0,48 0,50 -0.36 8 0,72 -1,5E6 4,14E6 -1,10E7 8,06E6


-0.36 7,71 0,72 -1,5E6 4,15E6 -1,11E7 8,13E6

hplus14 Equal variances assumed 1,58 0,24 0,75 8 0,47 9,6E5 1,27E6 -1,98E6 3,90E6


0,75 6,42 0,47 9,6E5 1,27E6 -2,11E6 4.036E6


86

b. Uji T berpasangan



Pair 1 hmin1 8,02E6 5 1,03E6 4,61E5

hplus1 8,08E6 5 2,93E6 1,31E6


N Correlation Sig.

Pair 1 hmin1 & hplus1 5 0,17 0,782

Paired Samples Test

Paired Differences

t df

Sig. (2-

tailed)

Mean

Std.

Deviation

Std. Error

Mean

95% Confidence Interval

of the Difference

Lower Upper

Pair 1 hmin1 -

hplus1 -6,00E4 2,94E6 1,31E6 -3,71E6 3,59E6 -0,04 4 0,966



Pair 1 hmin1 8,02E6 5 1,03E6 4,61E5

hplus7 1,46E7 5 5,89E6 2,63E6


N Correlation Sig.

Pair 1 hmin1 & hplus7 5 0,889 0,044


87

Paired Samples Test

Paired Differences

t df

Sig. (2-

tailed)

Mean

Std.

Deviation

Std. Error

Mean


of the Difference

Lower Upper

Pair 1 hmin1 -

hplus7 -6,54E6 4,99E6 2,23E6 -1,27E7 -3,37E5 -2,98 4 0,043



Pair 1 hmin1 8,02E6 5 1.03E6 4.61E5

hplus14 7,36E6 5 1.43E6 6.40E5


N Correlation Sig.

Pair 1 hmin1 & hplus14 5 0,90 0,034

Paired Samples Test

Paired Differences

t df

Sig. (2-

tailed)

Mean

Std.

Deviation

Std. Error

Mean

95% Confidence

Interval of the

Difference

Lower Upper

Pair 1 hmin1 - hplus14 6,60E5 6,63E5 2,96E5 -1,63E5 1,48E6 2,22 4 0,090


88

3. Hasil analisis statistik jumlah sel darah putih

a. Uji T tidak berpasangan

Group Statistics


hplus1 Perlakuan 5 7,62E3 1248,29 558,26

nonperlakuan 5 7,71E3 855,42 382,56

hmin1 Perlakuan 5 4,52E3 1645,29 735,80

nonperlakuan 5 5,36E3 551,58 246,68


nonperlakuan 5 8,60E3 1200,52 536,89


nonperlakuan 5 7,68E3 124,27 555,57


Levene's Test

for Equality of


F Sig. t df

Sig. (2-

tailed)

Mean

Difference

Std. Error

Differenc

e

95% Confidence

Interval of the

Difference

Lower Upper

hplus1 Equal variances assumed 0,44 0,53 -0,13 8 0,90 -90,00 676,76 -1650,60 1470,60


-0,13 7,08 0,90 -90,00 676,76 -1686,70 1506,71

hmin1 Equal variances assumed 19,22 0,02 -1,08 8 0,31 -840,00 776,05 -2629,56 949,57


-1,08 4,89 0,33 -840,00 776,05 -2848,72 1168,73

hplus7 Equal variances assumed 0,02 0,90 -0,69 8 0,51 -530,00 770,65 -2307,12 1247.,29


-0,69 7,99 0,51 -530,00 770,65 -2307,38 1247,38

hplus14 Equal variances assumed .043 .840 0,33 8 0,75 250,00 757,33 -1496,41 1996,41


0,33 7,954 0,75 250,00 757,33 -1498,18 1998,19


89




Pair 1 hmin1 4,52E3 5 1645,30 735,80

hplus1 7,62E3 5 1248,30 558,26


N Correlation Sig.

Pair 1 hmin1 & hplus1 5 0,667 0,219

Paired Samples Test

Paired Differences

t df

Sig. (2-

tailed)

Mean

Std.

Deviation

Std. Error

Mean


the Difference

Lower Upper

Pair 1 hmin1 -

hplus1 -3,10E3 1235,42 552,50 -4633,97 -1566,03 -5,61 4 0,005



Pair 1 hmin1 4,52E3 5 1645,36 735,80

hplus7 8,07E3 5 1236,22 552,87


N Correlation Sig.

Pair 1 hmin1 & hplus7 5 -0,51 0,379


90

Paired Differences

t df

Sig. (2-

tailed)

Mean

Std.

Deviation

Std. Error

Mean


the Difference

Lower Upper

Pair 1 hmin1 - hplus7 -3,55E3 2512,72 1123,72 -6669,95 -430,05 -3,159 4 0,034



Pair 1 hmin1 4,52E3 5 1645,30 735,80

hplus14 7,93E3 5 1150,87 514,68


N Correlation Sig.

Pair 1 hmin1 & hplus14 5 0,138 0,825

Paired Samples Test

Paired Differences

t df

Sig. (2-

tailed)

Mean

Std.

Deviation

Std. Error

Mean


the Difference

Lower Upper

Pair 1 hmin1 -

hplus14 -3,41E3 1873,63 837,91 -5736,42 -1083.58 -4,07 4 0,015


91

4. Hasil analisis statistic enzim GPT


Group Statistics


hmin1 Perlakuan 5 33,30 21,96 9,82

nonperlakuan 5 21,14 2,80 1,25

hplus1 Perlakuan 5 38,52 17,81 7,97



nonperlakuan 5 35,26 14,59 6,52




Levene's Test

for Equality of


F Sig. t df

Sig. (2-

tailed)

Mean

Differen

ce

Std.

Error

Differen

ce

95% Confidence

Interval of the

Difference

Lower Upper

hmin1 Equal variances assumed 26,02 0,00 1.228 8 0,25 12,16 9,90 -10,67 34,99


1.228 4,13 0,28 12.16 9,90 -14,99 39,30

hplus1 Equal variances assumed 3,28 0,11 1.034 8 0,33 8,62 8,33 -10,60 27,84


1.034 4,75 0,35 8,62 8,33 -13,15 30,39



-0,970 4,96 0,38 -6,70 6,91 -24,51 11,11

hplus14 Equal variances assumed 0,98 0,35 2,024 8 0,08 8,28 4,09 -1,15 17,71


2,024 5,97 0,09 8,28 4,09 -174 18,30


92




Pair 1 hmin1 33,30 5 21,96 9,82

hplus1 38,52 5 17.82 7,96


N Correlation Sig.

Pair 1 hmin1 & hplus1 5 0,494 0,397

Paired Samples Test

Paired Differences

t df

Sig. (2-

tailed)

Mean

Std.

Deviation

Std. Error

Mean


of the Difference

Lower Upper

Pair 1 hmin1 - hplus1 -5,22 20,32 9,09 -30,45 20,01 -0,574 4 0,596



Pair 1 hmin1 33,30 5 21,96 9,82

hplus7 28,56 5 5,08 2,27


N Correlation Sig.

Pair 1 hmin1 & hplus7 5 -0,407 0,497


93

Paired Samples Test

Paired Differences

t df

Sig. (2-

tailed)

Mean

Std.

Deviation

Std. Error

Mean

95% Confidence

Interval of the

Difference

Lower Upper

Pair 1 hmin1 - hplus7 4,74 24,47 10,94 -25,65 35,13 0,433 4 0,687



Pair 1 hmin1 33,30 5 21,96 9,82

hplus14 28,98 5 8,14 3,64

Paired Samples Test

Paired Differences

t df Sig. (2-tailed)

Mean

Std.

Deviation

Std. Error

Mean


the Difference

Lower Upper

Pair 1 hmin1 -

hplus14 4,32 24,60 11,00 -26.23 34,87 0,39 4 0,715


N Correlation Sig.

Pair 1 hmin1 & hplus14 5 -0,159 0,799


94

5. Analisis statistic enzim GOT


Group Statistics


hmin1 Perlakuan 5 85,94 24,11 10,78

nonperlakuan 5 66,44 14,80 6,62

hplus1 Perlakuan 5 118,5 40,81 18,25

nonperlakuan 5 98,16 12,20 5,45

hplus7 Perlakuan 5 107,38 21,78 9,73

nonperlakuan 5 139,84 41,91 18,74

hplus14 Perlakuan 5 99,26 23,33 10,43

nonperlakuan 5 94,78 18,12 8,10


Levene's Test

for Equality of


F Sig. t df

Sig.

(2-

tailed)

Mean

Differenc

e

Std.

Error

Differenc

e

95% Confidence

Interval of the

Difference

Lower Upper

hmin1 Equal variances assumed 3,28 0,11 1,54 8 0,16 19,50 12,65 -9,68 48,67


1,54 6,64 0,17 19,50 12,65 -10,75 49,75



1,06 4,71 0,34 20,36 19,04 -29,53 70,25



-1,53 6,01 0,17 -32,46 21,12 -84,11 19,19



0,34 7,54 0,74 4,48 13,21 -26,31 35,27


95




Pair 1 hmin1 85,940 5 24,11 10,78

hplus1 118,52 5 40,81 18.25


N Correlation Sig.

Pair 1 hmin1 & hplus1 5 -0,302 0,621

Paired Samples Test

Paired Differences

t df

Sig. (2-

tailed)

Mean

Std.

Deviation

Std. Error

Mean

95% Confidence

Interval of the

Difference

Lower Upper

Pair 1 hmin1 - hplus1 -32,58 53,30 23,83 -98,76 33,60 -1,36 4 0,244



Pair 1 hmin1 85,94 5 24,11 10,78

hplus7 107,38 5 21,77 9,74


N Correlation Sig.

Pair 1 hmin1 & hplus7 5 0,279 0,649


96

Paired Samples Test

Paired Differences

t df

Sig. (2-

tailed)

Mean

Std.

Deviation

Std. Error

Mean

95% Confidence

Interval of the

Difference

Lower Upper

Pair 1 hmin1 - hplus7 -21,44 27,61 12,35 -55,73 12,85 -1,73 4 0,158



Pair 1 hmin1 85,94 5 24,11 10,78

hplus14 99,26 5 23,33 10,43


N Correlation Sig.

Pair 1 hmin1 & hplus14 5 -0,372 0,538

Paired Samples Test

Paired Differences

t df

Sig. (2-

tailed)

Mean

Std.

Deviation

Std. Error

Mean


of the Difference

Lower Upper

Pair 1 hmin1 -

hplus14 -13,32 39,28 17,57 62,10 35,46 -0,75 4 0,491


97

6. Analisis statistik urea darah


Group Statistics


hmin1 Perlakuan 5 36,80 14,20 6,35


hplus1 Perlakuan 5 43,40 29,33 13,11







Levene's Test for Equality of


F Sig. t df Sig. (2-tailed)

Mean Difference

Std. Error

Difference

95% Confidence Interval of the

Difference Lower Upper

hmin1 Equal variances assumed 1,654 0, 234 0,25 8 0,80 2,00 7,73 -15,84 19,84

Equal variances not assumed 0,25 7,13 0,80 2,00 7,73 -16,22 20,22 hplus1 Equal variances assumed 3,871 0,085 0,91 8 0,38 12,20 13,36 -18,60 43,00

Equal variances not assumed 0,91 4,29 0,40 12,20 13,36 -23,89 48,29 hplus7 Equal variances assumed 0,062 0,810 -1,33 8 0,21 -3,80 2,84 -10,37 2,77

Equal variances not assumed -1,33 7,54 0,22 -3,80 2,84 -10,44 2,84 hplus14 Equal variances assumed 0,473 0,511 1,89 8 0,09 5,80 3,05 -1,24 12,84

Equal variances not assumed 1,89 7,27 0,09 5,80 3,05 -1,37 12,97


98




Pair 1 hmin1 36,80 5 14,20 6,35

hplus1 43,40 5 29,33 13,11


N Correlation Sig.

Pair 1 hmin1 & hplus1 5 0,553 0,334

Paired Samples Test

Paired Differences

t df

Sig. (2-

tailed)

Mean

Std.

Deviation

Std. Error

Mean

95% Confidence

Interval of the

Difference

Lower Upper

Pair 1 hmin1 - hplus1 -6,60 24,52 10,96 -37,04 23,84 -0,60 4 0,580



Pair 1 hmin1 36,80 5 14,20 6,35

hplus7 27,60 5 5,02 2,24


N Correlation Sig.

Pair 1 hmin1 & hplus7 5 0,55 0,331


99

Paired Samples Test

Paired Differences

t df

Sig. (2-

tailed)

Mean

Std.

Deviation

Std. Error

Mean

95% Confidence

Interval of the

Difference

Lower Upper

Pair 1 hmin1 - hplus7 9,20 12,15 5,43 -5,89 24,29 1,69 4 0,166



Pair 1 hmin1 36,80 5 14,20 6,35

hplus14 34,00 5 4,00 1,78


N Correlation Sig.

Pair 1 hmin1 & hplus14 5 -0,075 0,905

Paired Samples Test

Paired Differences

t df

Sig. (2-

tailed)

Mean

Std.

Deviation

Std. Error

Mean

95% Confidence

Interval of the

Difference

Lower Upper

Pair 1 hmin1 -

hplus14 2,80 15,03 6,72 -15,87 21,47 0,416 4 0,699


100

7. Hasil analisis statistik bobot organ ginjal kanan (uji t tidak berpasangan)

Group Statistics


bobotorgan perlakuan 5 0,57 0,05 0,02



Levene's Test for

Equality of


F Sig. t df

Sig.

(2-

tailed)

Mean

Differen

ce

Std.

Error

Differen

ce

95%

Confidence

Interval of the

Difference

Lower Upper

bobotorgan Equal variances

assumed 0,03 0,867 0,47 8 0,64 0,01 0,03 -0,06 0,10

Equal variances

not assumed

0,47 7,84 0,64 0,01 0,03 -0,06 0,10


101

8. Hasil analisis statistik bobot organ ginjal kiri ( uji T tidak berpasangan)

Group Statistics

Kelompokuji N Mean Std. Deviation Std. Error Mean

bobotorgan Perlakuan 5 0,56 0,05 0,02

Nonperlakuan 5 0,52 0,08 0,03


Levene's Test

for Equality of


F Sig. t df

Sig.

(2-

tailed)

Mean

Difference

Std. Error

Difference

95% Confidence

Interval of the

Difference

Lower Upper

bobotorgan Equal variances assumed 0,147 0,711 0,86 8 0,41 0,03 0,04 -0,06 0,13

Equal variances not assumed 0,86 6,65 0,41 0,03 0,04 -0,06 0,14


102

9. Hasil analisis statistik bobot organ hati ( uji T tidak berpasangan)

T-Test

Group Statistics

Kelompokuji N Mean Std. Deviation Std. Error Mean

bobotorgan Perlakuan 5 7,33 0,46 0,20

Nonperlakuan 5 7,32 1,15 0,51


Levene's Test for

Equality of


F Sig. t df

Sig.

(2-

tailed)

Mean

Differen

ce

Std.

Error

Differenc

e

95%

Confidence

Interval of the

Difference

Lower Upper

bobotorgan Equal variances assumed 3,158 0,11 0,00 8 0,993 0,00 0,55 -1,28 1,29

Equal variances not

assumed

0,00 5,22 0,993 0,00 0,55 -1,41 1,41


lampiranrepository.ump.ac.id/1728/8/fitri kurniawati, lampiran...7 5500 7650 6950 6800 8 5200 6900...

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