Download - Sequenching by hybridization
Sequenching by Hybridization
(SBH)
The next generation of sequenching technologies
Ziebolz B, 2006, Next generation sequenching technologies, BTi.
Sequenching by HybridizationPrinsip:
Perbedaan hibridisasi dari pelacak oligonukleotidayang dapat digunakan untuk membaca kode genetik dari DNA target.
Pendekatan:1. Imobilisasi DNA pada membran yang akan menginisiasi hibridisasi yang
dideteksi oleh probe oligonukleotida pendek.2. Hibridisasi DNA ke microfabricated tilling arrays immobilized
oligonucleotide, with > 100.000 copies of each induvidual of feature.
Mekanisme SBH
To resequence a given base, four features are present on the microarray, each identical except for a different nucleotide at the query position (the central base of 25-bp oligonucleotides). Genotyping data at each base are obtained through the differential hybridization of genomic DNA to each set of four features.
Jay Shendure*, Robi D.Mitra‡, Chris Varma* and George M. Church*, 2005, ADVANCED SEQUENCING TECHNOLOGIES: METHODS AND GOALS, Nature Review, Volume 5.
Lanjutan…
MIcroarrayDNA microarray analysis is one of the fastest-growing new technologies in the field of genetic research. The basis of DNA microarray is sequencing by hybridization.
Aplikasi SBHIdentification of FVIII gene mutations in patients with hemophilia A