datpeng praktikum 10
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VII. Data PengamatanA. Data Pengamatan Wet Lab
PerlakuanKelompokKadar Glukosa Darah mg/dL
BB hewan (g)t = 0t = 30t = 60
Kontrol Negatif120.61027250
(PGA)225.5169261189
327.5131124152
416.381--
524.9106126132
Jumlah589583523
Rata-rata117.8145.75130.75
UJI I124.71087150
228.110810589
325.612210098
417.39812957
523.611010598
Jumlah546510392
Rata-rata109.210278.4
UJI II122.510612479
228.61608180
323.9898682
428.811210672
526.9799467
Jumlah546491380
Rata - rata109.298.276
Perhitungan
Kadar glukosa relatif kontrol negatif
Menit 30
Kadar glukosa relatif=Kadar glukosa menit 30x100%
Kadar glukosa menit 0
=145.75 x 100%
117.8
=123.73 %
Menit 60Kadar glukosa relatif=Kadar glukosa menit 60x100%
Kadar glukosa menit 0
=130.75 x 100%
117.8
=110.99 %
Kadar glukosa relatif uji glibenklamid (I) Menit 30Kadar glukosa relatif=Kadar glukosa menit 30x100%
Kadar glukosa menit 0
=102 x 100%
109.2
=93.41 %
Menit 60
Kadar glukosa relatif=Kadar glukosa menit 60x100%
Kadar glukosa menit 0
=78.4 X100%
109.2
=71.79 %
Kadar glukosa relatif uji glibenklamid (II) Menit 30Kadar glukosa relatif=Kadar glukosa menit 30x100%
Kadar glukosa menit 0
=98.2 x 100%
109.2
=89.93%
Menit 60
Kadar glukosa relatif=Kadar glukosa menit 60x100%
Kadar glukosa menit 0
=76 X100%
109.2
=69.60 %
% Penurunan glukosa untuk UJI I Menit 30% Penurunan glukosa=(Kadar glukosa relatif kontrol menit 30 - Kadar glukosa relatif uji menit 30)
Kadar glukosa relatif kontrol menit 30
=145.75 - 102x 100%
145.75
=30,02 %
Menit 60
% Penurunan glukosa=(Kadar glukosa relatif kontrol menit 60 - Kadar glukosa relatif uji menit 60)
Kadar glukosa relatif kontrol menit 60
=130.75 78.4x 100%
130.75
=40,04 %
Obat uji I =
% Penurunan glukosa untuk UJI I Menit 30% Penurunan glukosa=(Kadar glukosa relatif kontrol menit 30 - Kadar glukosa relatif uji menit 30)
Kadar glukosa relatif kontrol menit 30
=145.75 98.2x 100%
145.75
=32,62 %
Menit 60
% Penurunan glukosa=(Kadar glukosa relatif kontrol menit 60 - Kadar glukosa relatif uji menit 60)
Kadar glukosa relatif kontrol menit 60
=130.75 76x 100%
130.75
=41,87 %
Obat Uji II
Analisis Anava
a. Model Linear
Yij= + i+ ij.
Yij = Depresi mencit (lama waktu diam) yang mendapat obat ke-I ulangan ke-j terhadap lingkungan
= rataan umum
i = pengaruh obat ke-i
ij. = pengaruh galat dari obat ke-I ulangan ke-jb. Hipotesis
H0 : 1 = 2 = 3 = 0
Tidak ada pengaruh jenis obat terhadap depresi mencitH1 : paling sedikit ada satu I dimana 1 0
Ada pengaruh obat terhadap glukosa
c. Analisis Ragam
Faktor Koreksi
Sum of Square TotalSSt = Yij2 FKSSt = (117.82 + 145.75+ 130.75 + 109.2+ 102+ 78.4 2 + 109.22 + 98.22 + 76 2) = 108034.5 103963.25 = 4071.295 Sum of Square Treatment
SSTreat = 2588.82 Sum Square of ErrorSSE = SSTot SSTreatSSE =4071.295 2588.82= 1482.475 Degree of Freedom
df total = N 1 = 9 1 = 8
df Treat = t 1 = 3 1 = 2
df Error = df total df treat = 8 -2 = 6 Mean of Square Treatment (MSTreat)
Mean of Square Error (MSE)
F hitung
Tabel ANAVASource of VariancedfSum of SquaresMean of SquaresF hitungF 0.05 (2,6)
Treatment25.14
Error6
Total84071.295
i. Kesimpulan
F table = 5.14
Tolak H0 jika F hitung > F table
5.24 > 5.143 ( tolak H0
Kesimpulan: ada pengaruh pemberian obat terhadap kadar glukosaDRY LAB
1. Kurva baku glukosa
TabungOptical densityGlucose (mg/dL)
10.330
20.560
30.690
40.8120
51150
Tube Optical densityGlucose (mg/dL)Insulin Saline Aloxan
1086NoYesNo
20129NoNoYes
3087YesYesNo
4096YesNoYes
_1493704086.xlsChart1
117.8109.2109.2
145.7510298.2
130.7578.476
Kontrol negatif
Glibenklamid (I)
Glibenklamide (II)
Waktu (menit)
Kadar glukosa relatif (%)
Grafik Kadar Glukosa Relatif Terhadap Waktu
Sheet1
Kontrol negatifGlibenklamid (I)Glibenklamide (II)
0117.8109.2109.2
30145.7510298.2
60130.7578.476
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