data pengamatan
DESCRIPTION
data pengamatanTRANSCRIPT
DATA PENGAMATAN
Uji kesesuaian sistem
Penyuntikan Waktu Retensi Luas Area
1 3,990 43670610
2 3,903 44146754
3 3,883 43755552
4 3,880 43966139
5 3,867 45006572
6 3,837 45383519
7 3,820 45376470
Rata-Rata 3,883 44472230,86
SD 0,055073976 758496,7259
SBR 1,418387909 1,705551332
Uji kualitatif
Kromatogram larutan standar
Kromatogram larutan uji
Waktu retensi puncak sampel uji parasetamol (uji 1 = 3,800 menit; uji 2 = 3,797 menit)
mendekati nilai waktu retensi puncak larutan standar 3,793 menit.
Konsentrasi Larutan Stok Baku Pembanding Parasetamol
25,59mg50ml
=25,59mg0,05 l
=511,8 ppm
Larutan 0,2 ml
V1M1=V2M2
0,2 ml . 511,8 ppm = 10 ml . M2
M2 = 0,2ml .511,8 ppm
10ml = 10,236 ppm
Larutan 0,8 ml
V1M1=V2M2
0,8 ml . 511,8 ppm = 10 ml . M2
M2 = 0,8ml .511,8 ppm
10ml = 40,944 ppm
Larutan 0,4 ml
V1M1=V2M2
0,4 ml . 511,8 ppm = 10 ml . M2
M2 = 0,4ml .511,8 ppm
10ml = 20,472 ppm
Larutan 1,0 ml
V1M1=V2M2
1,0 ml . 511,8 ppm = 10 ml . M2
M2 = 1,0ml .511,8 ppm
10ml = 51,18 ppm
Larutan 0,6 ml
V1M1=V2M2
0,6 ml . 511,8 ppm = 10 ml . M2
M2 = 0,6ml .511,8 ppm
10ml = 30,708 ppm
Larutan 1,2 ml
V1M1=V2M2
1,2 ml . 511,8 ppm = 10 ml . M2
M2 = 1,2ml .511,8 ppm
10ml = 61,416 ppm
Tabel Konsentrasi
Konsentrasi (ppm) Luas Area (Auc/Voltz)
10,236 9851878
20,472 17237161
30,708 24468940
40,944 33504609
51,18 44162357
61,416 51033528
0 1 2 3 4 5 6 70
10000000
20000000
30000000
40000000
50000000
60000000
f(x) = 8449128.77142857 x + 471128.133333329R² = 0.995699140598713
Kurva Kalibrasi Parasetamol
Series2Linear (Series2)
Konsentrasi (ppm)
Luas
are
a
Kadar parasetamol dalam tablet parasetamol (cara kurva kalibrasi)
Sampel Uji Tablet 1
50mg4 .500mg
x 2,42gr=60,5mg
Pengenceran
60,5mg100ml
=605 ppm
V1.M1=V2.M2
2 ml . 605 ppm = 25 ml . M2
M2 = 2ml .605 ppm
25ml = 48,4 ppm
Perhitungan Kadar
Y1 = bx + a
Y1 = 825433x + 471128
33249458 = 82543x + 471128
32778330 = 825433X
X = 39,71 ppm
% Kadar
X1Pengenceran
x 100%
39 ,71 ppm48,4 ppm
x 100 %=82,05 %
Sampel Uji Tablet 2
50mg4 .500mg
x 2,45gr=61,25mg
Pengenceran
61,25mg100ml
=612,5 ppm
V1.M1=V2.M2
2 ml . 612,5 ppm = 25 ml . M2
M2 = 2ml .612,5 ppm
25ml = 49 ppm
Perhitungan Kadar
Y2 = bx + a
Y2 = 825433x + 471128
33569331 = 82543x + 471128
33098203 = 825433X
X = 40,1 ppm
% Kadar
X1Pengenceran
x 100%
40,1 ppm49 ppm
x 100 %=81,837 %
Kadar parasetamol dalam tablet parasetamol (One Point)
Sampel uji tablet 1
Cu = lulsx Cs
Cu = 3324945817237161
x20,472 ppm=¿39,37 ppm
Sampel uji tablet 1
Cu = lulsx Cs
Cu = 3356933117237161
x20,472 ppm=¿39,87 ppm
%kadar
xpengenceran( ppm)
=x 100 %
39,3748,4
x 100 %=81,35 %
%kadar
xpengenceran( ppm)
=x 100 %
39,8749
x 100 %=81,367 %