DAFTAR ISI

DAFTAR ISI.............................................................................................................i

DAFTAR GAMBAR..............................................................................................iii

DAFTAR TABEL...................................................................................................vi

BAB 1. PENDAHULUAN......................................................................................1

1.1 Latar Belakang.........................................................................................1

1.2 Rumusan..................................................................................................2

1.3 Tujuan......................................................................................................2

BAB 2. TINJAUAN PUSTAKA.............................................................................3

2.1 Esterogen.................................................................................................3

2.2 Reseptor Esterogen .................................................................................4

2.3 Michigan Cancer Foundation -7 (MCF-7)...............................................5

BAB 3. METODOLOGI PENELITIAN..................................................................7

3.1 Objek Penelitian......................................................................................7

3.2 Prosedur Kerja.........................................................................................8

3.2.1 Pengambilan data prediktor............................................................8

3.2.2 Teknik pemisahan data secara acak................................................8

3.3.2 Analisis regresi multilinier.............................................................8

3.3.3 Hewan Uji.......................................................................................6

BAB 4. HASIL DAN PEMBAHASAN.................................................................10

4.1 Estradiol...............................................................................................10

4.2 Ikatan estradiol dan ERα......................................................................10

4.3 Hubungan Kuantitatif Struktur dan Aktivitas Senyawa

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Turunan Estradiol Perhitungan Metode Semiempiris Aml.....................13

4.3.1 Hasil perhitungan deskriptor.......................................................13

4.3.2 Kajian korelasi aktivitas dan prediktor.......................................14

4.3.3 Hasil analisis regresi multilinier QSAR......................................14

4.3.4 Perumusan persamaan QSAR akhir............................................17

4.4 Sifat Turunan Estradiol........................................................................19

4.4.1 Etinil Estradiol............................................................................19

4.4.2 Estradiol-17β-hemuksinat...........................................................20

4.4.3 2- Metoksi Estradiol....................................................................21

4.4.4 Estradiol Benzoat........................................................................22

4.4.5 Dietilstilbestrol............................................................................23

BAB. 5 PENUTUP.................................................................................................24

5.1 Kesimpulan...........................................................................................24

5.2 Saran......................................................................................................24

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DAFTAR GAMBAR

Gambar 1. Skema Aktivasi reseptor esterogen oleh esterogen

sampai menghasilkan efek....................................................................5

Gambar 2.Sel MCF-7...............................................................................................6

Gambar 3. Struktur Dasar senyawa turunan estradiol..............................................7

Gambar 4. Ikatan 17β-estradiol dengan Erα..........................................................11

Gambar 5. Mekanisme kerja estradiol...................................................................12

Gambar 6. Grafik korelasi log (1/IC50)eksperimen vs log (1/IC50)prediksi model

persamaan 4 dari senyawa fitting (a) dan senyawa uji (b)...................17

Gambar 7 . Grafik korelasi log (1/IC50)eksperimen vs log (1/IC50)prediksi

menggunakan 19 seri senyawa estradiol.............................................18

Gambar 8. Struktur Senyawa Etinil Estradiol........................................................19

Gambar 9. Struktur Senyawa Estradiol-17β- hemuksinat......................................20

Gambar 10. Struktur Senyawa Metoksi Estradiol..................................................21

Gambar 11. Struktur Senyawa Estradiol Benzoat..................................................22

Gambar 12. Struktur senyawa Dierilstibestrol.......................................................23

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DAFTAR TABEL

Tabel 1. Data kadar Penghambatan IC50 dan Log (1/IC50) eksperimen...................7

Tabel 2. Data muatan atom, momen dwikutub dan log P hasil perhitungan

metode semiempiris AM1..........................................................................9

Tabel 3. Korelasi antar variabel.............................................................................14

Tabel 4. Model persamaan QSAR terpilih turunan estradiol hasil SPSS..............15

Tabel 5. Nilai PRESS internal dan log (1/IC50) Prediksi senyawa fitting.........................16

Tabel 6. Nilai PRESS eksternal dan log (1/IC50) senyawa uji.....................................16

Tabel 7. Nilai log (1/IC50) prediksi..............................................................................19

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