biotek bakteri gram

7/24/2019 Biotek Bakteri Gram

1/20

LAPORAN PRAKTIKUM MIKROBIOLOGI FARMASI

PEWARNAAN SEDERHANA

Rabu, 4 Maret 2015

Kelomo! IIRabu, Pu!ul 1"#"0 $ 1%#"0 &IB

Nama NPM

Gu'tur A(ta)a 2%01101"0110

LABORATORIUM MIKROBIOLOGI FARMASIFAKULTAS FARMASI

UNI*+RSITAS PA-A-ARAN

2015

I# Tu.ua'

N/la/ TT

S/'ta3 Be'e/tu(3


2/20

Me'6amat/ u!ura', be'tu! a' (tru!tur7(tru!tur terte'tu ar/ ba!ter/, e'6a'

me'66u'a!a' (atu maam 8at e)ar'a#

II# Pr/'(/ a(ar

1# Te!'/! A(et/(

Te!'/! a(et/( atau (ter/l aala (uatu (/(tem ara be!er.a ra!te!3 a'6

me'.a6a (ter/l/ta( !et/!a me'a'6a'/ e'6!ultura' m/!roor6a'/(me u'tu!

me'e6a !o'tam/'a(/ teraa !ultur m/!roor6a'/(me a'6 //'6/'!a'#

2# Pe)ar'aa' Seera'a

Pember/a' )ar'a aa ba!ter/ e'6a' me'66u'a!a' laruta' tu'66al (uatu

e)ar'a aa la/(a' t//( atau e(a/' a'6 tela /9/!(a(/#

"# I!ata' Io'

Pr/'(/ a(ar ar/ e)ar'aa' /'/ aala aa'a /!ata' /o' a'tara !omo'e'

(eluler ar/ ba!ter/ e'6a' (e'a)a a!t/9 ar/ e)ar'aa' a'6 /(ebut

!romo6e'# Ter.a/ /!ata' /o' !are'a aa'a muata' l/(tr/! ba/! aa !omo'e'

(eluler mauu' aa e)ar'aa'# Bera(ar!a' aa'a muata' /'/ ma!a aat

/bea!a' e)ar'a a(am a' e)ar'a ba(a#

III# Teor/ a(ar

Mel/at a' me'6amat/ ba!ter/ alam !eaa' /u (a'6at (ul/t, !are'a (ela/'

ba!ter/ /tu t/a! ber)ar'a .u6a tra'(ara' a' (a'6at !e/l# U'tu! me'6ata(/ al

ter(ebut ma!a /!emba'6!a' (uatu te!'/! e)ar'aa' (el ba!ter/ /'/ merua!a'

(ala (atu ara a'6 al/'6 utama alam e'el/t/a'7e'el/t/a' m/!rob/olo6/#

)/.o(eutro#1::;3#

Pe)ar'aa' (eera'a merua!a' te!'/! e)ar'aa' a'6 al/'6 ba'a!

/6u'a!a'# Mel/at a' me'6amat/ ba!ter/ alam !eaaa' /u (a'6at (ul/t,

!are'a (ela/' ba!ter/ /tu t/a! ber)ar'a .u6a tra'ara' a' (a'6at !e/l# U'tu!

me'6ata(/ al ter(ebut ma!a /!emba'6!a' (uatu te!'/! e)ar'aa' (el ba!ter/,

(e/'66a (el aat terl/at .ela( a' mua /amat/#


3/20


(eluler ar/ ba!ter/ e'6a' (e'a)a a!t/9 ar/ e)ar'aa' a'6 /(ebut !romo6e'#

Ter.a/ /!ata' /o' !are'a aa'a muata' l/(tr/! ba/! aa !omo'e' (eluler

mauu' aa e)ar'aa'# Bera(ar!a' aa'a muata' /'/ ma!a aat /bea!a'

e)ar'a a(am mauu' e)ar'a ba(a# Met, 20143#

Pe)ar'aa' a(am Merua!a' e)ar'aa' a'6 me'66u'a!a' (atu maam 8at

)ar'a e'6a' tu.ua' a'a u'tu! mel/at be'tu! (el# Aau' 8at )ar'a a'6

/a!a/ alam e)ar'aa' o(/t/9 aala met/le' b/ru a' a/r 9ur!(/'# &era,

20113#

Pe)ar'aa' Ba(a atau 'e6at/9 merua!a' metoe e)ar'aa' u'tu! me)ar'a/

ba!ter/ teta/ me)ar'a/ latar bela!a'6'a me'.a/ /tam 6ela# Paa e)ar'aa'

/'/ m/!roor6a'/(me !el/ata' tra'(ara' tembu( a'a'63# Te!'/! /'/ ber6u'a

u'tu! me'e'tu!a' mor9olo6/ a' u!ura' (el# Metoe /'/ me'66u'a!a' at

'/6ro(/' atau t/'ta /'a# &era, 20113#

La'6!a7la'6!a utama alam er(/aa' (e(/ma' u'tu! emer/!(aa'

m/!ro(!o/! aala=

7 Pe'emata' ole(a' atau la/(a' (e(/me' aa !aa ob.e!#

7 F/!(a(/ ole(a' aa !aa ob.e!#

7 Al/!a(/ e)ar'a tu'66al e)ar'aa' (eera'a3 atau (era'6!a/a' laruta'

e)ar'a atau rea6e' e)ar'aa' /9ere'(/al3#

Pel8ar, 1:;%3#

I*# Alat a' Baa'

4#1 Alat1# Ba! e)ar'a

2# Botol (emrot

"# >a)a' Petr/

4# Kaa Ob.e!


4/20

5# Kaa(

%# Kerta( (ar/'6

?# M/!ro(!o ma.emu!

;# O(e

:# Pemba!ar (/r/tu(

Gambar Alat

1 2 "

4 5 %

? ; :

4#2 Baa'

1# A/r (ul/'6

2# Al!ool ?0 @

"# B/ru met/le'


5/20

4# >G* >arbol Ge't/a' */olet3

5# Karbol Fu!(/'

%# M/'a! emer(/

?# Samel a/r l/ur

*# Pro(eur

Pertama, Samel a/r l/ur /ole(!a' / ata( !aa obe! a'6 ber(/ (erta beba(

lema!# Setela /'6/', rearat /leta!!a' / ata( ba! )ar'a# Lalu, ole(a' ter(ebut

/6e'a'6/ e'6a' (ala (atu ar/ 8at )ar'a# B/la /6u'a!a' !arbol 9u!(/', ole(a'

/b/ar!a' ter)ar'a/ (elama 5 me'/t# B/la /6u'a!a' b/ru met/le', ole(a' /b/ar!a'

ter)ar'a/ (elama 172 me'/t# B/la /6u'a!a' !arbol 6e't/a' /olet, ole(a' /b/ar!a'

ter)ar'a/ (elama 157"0 et/!# Lalu, 8at )ar'a a'6 berleb/ ar/ rearat

/tua'6!a' a' /b/la( e'6a' a/r (ama/ a/r b/la(a' ber)ar'a uat# Kemu/a',

rearat /!er/'6!a' e'6a' !erta( (ar/'6# Lalu, (e/!/t m/'a! emer(/ /tete(!a'

aa rearat, lalu /er/!(a / ba)a m/!ro(!o# Mula/la e'6a' obe!t/9

ber!e!uata' tere'a 10# lalu /6a't/ e'6a' le'(a obe!t/9 ber!e!uata' 100#

Prearat /amat/ a' /6ambar!a' a(/l'a# Pe)ar'aa' e'6a' !arbol 9u!(/', (el

ba!ter/ a!a' ber)ar'C mera# B/la /)ar'a/ e'6a' !arbol 6e't/a' /olet, (el

ba!ter/ a!a' ber)ar'a u'6u, Sea'6 e)ar'aa' e'6a' b/ru met/le', (el ba!ter/

a!a' ber)ar'a b/ru#

*I#


6/20

Ba!ter/ Coccus

Pe)ar'a = Met/l B/ru

Prearat = A/r l/ur

Perbe(ara' = 10 D 100

Ie't/9/!a(/ ba!ter/ = Coccus,Bacillus

*II# Pembaa(a'

alam ra!t/!um /'/, /la!u!a' erobaa' e)ar'aa' (eera'a aa ba!ter/#

Tu.ua' ar/ ra!t/!um /'/ aala u'tu! me'6amat/ be'tu!, u!ura', a' (tru!tur

ba!ter/7ba!ter/ a'6 teraat aa (amel a/r l/ur e'6a' e)ar'aa'

me'66u'a!a' e)ar'a tu'66al aa m/!ro(!o#


7/20

aala u'tu! me'6a'6at!a' ba!ter/ a6ar ba!ter/ a'6 /u./ t/a! ber/'a7

/'a ar/ !aa ob.e!# alam mela!u!a' e'6ole(a' a/r l/ur aa !aa rearat,

al a'6 aru( /erat/!a' aala (ebelum mela!u!a' e'6ole(a', o(e a'6

/6u'a!a' aru( terleb/ aulu /a'a(!a' a6ar o(e ter(ebut beba( !o'tam/'a'

/b/ar!a' (e(aat /'66a /'6/' a6ar (u(e'(/ ba!ter/ t/a! mat/ !et/!a o(e

/6u'a!a' u'tu! me'6amb/l (amel a/r l/ur# >a)a' etr/ .u6a aru( /9/!(a(/

e'6a' emba!ar (/r/tu( a6ar !et/!a e'6amb/la' (amel ole o(e, a)a' etr/

beba( ar/ !o'tam/'a'# Setela rearat (ele(a/ /buat, rearat /leta!!a' /

ata( ba! )ar'a a' /ber/ e)ar'a, a/tu !arbol 9u!(/', >G*, atau b/ru met/le'#

Tu.ua' ember/a' )ar'a ter(ebut a6arr ba!ter/ aat teramat/ aa m/!ro(!o

!are'a e)ar'a ter(ebut ber/!ata' e'6a' ba!ter/ (e/'66a ba!ter/ a!a'

ber)ar'a a' aat /amat/# Setela /tu, 8at )ar'a berleb/ /bua'6 a' /b/la(

e'6a' a/r# Tu.ua' emb/la(a' a6ar 8at )ar'a a'6 t/a! ber/!ata' aa ba!ter/

t/a! mele!at aa rearat a'6 aat me'ebab!a' !e!el/rua' e'6amata'

aa m/!ro(!o 'a't/# Perlu /erat/!a' ba)a alam emb/la(a' rearat,

rearat aru( /b/la( (e/!/t em/ (e/!/t e'6a' a/r#


8/20

AFTAR PUSTAKA

)/.o(eutro, Rat'a S#, 1::;# M/!rob/olo6/ a(ar alam Pra!te! -a!arta= P#T

Grame/a Pu(ta!a#


9/20


10/20

LAPORAN PRAKTIKUM MIKROBIOLOGI FARMASI

PEWARNAAN GRAM

Rabu, 4 Maret 2015

Kelomo! IIRabu, Pu!ul 1"#"0 $ 1%#"0 &IB

Nama NPM

Gu'tur A(ta)a 2%01101"0110

LABORATORIUM MIKROBIOLOGI FARMASIFAKULTAS FARMASI

UNI*+RSITAS PA-A-ARAN

2015

I# Tu.ua'

N/la/ TT

S/'ta3 Be'e/tu(3


11/20

Me'6amat/ ua !elomo! ba!ter/ a/tu Gram positif a' Gram negatif, e'6a'

me'66u'a!a' ro(eur e)ar'aa' Gram, Memaam/ (et/a la'6!a a' rea!(/7

rea!(/ !/m/a a'6 ter.a/ alam ro(eur ter(ebut#

II# Pr/'(/ a(ar

1# Te!'/! A(et/(

Te!'/! a(et/( atau (ter/l aala (uatu (/(tem ara be!er.a ra!te!3 a'6

me'.a6a (ter/l/ta( !et/!a me'a'6a'/ e'6!ultura' m/!roor6a'/(me u'tu!

me'e6a !o'tam/'a(/ teraa !ultur m/!roor6a'/(me a'6 //'6/'!a'#

2# Pe)ar'aa' /99ere'(/al

Pro(eur e)ar'aa' a'6 me'am/l!a' erbeaa' / a'tara (el7(el m/!roba

atau ba6/a'7ba6/a' (el m/!roba /(ebut e)ar'aa' /99ere'(/al# B/a(a'a

/6u'a!a' leb/ ar/ (atu e)ar'a atau rea6e' e)ar'a#

"# I!ata' Io'


(eluler ar/ ba!ter/ e'6a' (e'a)a a!t/9 ar/ e)ar'aa' a'6 /(ebut

!romo6e'# Ter.a/ /!ata' /o' !are'a aa'a muata' l/(tr/! ba/! aa !omo'e'

(eluler mauu' aa e)ar'aa'# Bera(ar!a' aa'a muata' /'/ ma!a aat

/bea!a' e)ar'a a(am a' e)ar'a ba(a#

4# Ab(or(/

e'eraa' 9lu/a ole 9lu/a la/''a e'6a' membe'tu! (uatu laruta'#

III# Teor/ a(ar

Sel7(el m/!roor6a'/(me a'6 t/a! /)ar'a/ umum'a tama! am/r tembu(

a'a'6 tra'(ara'3 b/la /amat/ e'6a' m/!ro(!o aaa b/a(a /'66a (u!ar

/l/at !are'a (/tola(ma (el'a memu'a/ /'e!( b/a( a'6 am/r (ama e'6a'

/'e!( b/a( l/'6!u'6a''a a'6 ber(/9at a/r# Ko'tra( a'tara (el a' latar

bela!a'6'a aat /erta.am e'6a' ara me)ar'a/ (el7(el ter(ebut e'6a' 8at7

8at )ar'a


12/20

Pe)ar'aa' 6ram atau metoe 6ram aala (uatu metoe em/r/( u'tu!

membea!a' (e(/e( ba!ter/ me.a/ ua !elomo! be(ar, a/tu 6ram o(/t/9 a'

6ram 'e6at/9, bera(ar!a' (/9at !/m/a a' 9/(/! /'/'6 (el mere!a# Metoe

ter(ebut /ber/ 'ama bera(ar!a' e'emu'a, /lmu)a' e'mar!


13/20

leb/ mua ula /lu'tur!a' )ar'a'a# Sea'6!a' (el7(el a'6 (u!ar

/)ar'a/ a!a' leb/ (u!ar /lu'tur!a' )ar'a'a#

# Sub(trata

Merua!a' 8at )ar'a a(am atau ba(a aat berea!(/ e'6a' (e'a)a7

(e'a)a terte'tu# Ole !are'a /tu, (e'a)a7(e'a)a or6a'/! (eert/ rote/',

!arbo/rat, lema! a' a(am 'u!leat a!a' meme'6aru/ e)ar'aa'#

Bera(ar!a' .e'/( 8at )ar'a a'6 /(era ole (el, ma!a aat /bea!a' t/6a

maam (el a/tu= (el7(el a(/o9/l, ba(o/ll a' (ua'o9/l#

Sute.o, 1::13#

I*# Alat a' Baa'

4#1# Alat

1# Ba! e)ar'a

2# Botol (emrot

"# Kaa Ob.e!

4# Kaa(

5# Kerta( (ar/'6

%# M/!ro(!o ma.emu!

?# O(e

;# Pemba!ar (/r/tu(

Gambar Alat


14/20

1 2 "

4 5 %

? ;

4#2# Baa'

1# A/r 9u!(/'

2# A/r (ul/'6

"# Al!ool ?0 @

4# Al!ool :5@

5# >G* >arbol Ge't/a' */olet3

%# M/'a! emer(/?# Samel (u(e'(/ ba!ter/E. coli a'B. subtilis

*# Pro(eur

Kaa obe! a'6 ber(/ /(e/a!a' a' ole(a' ar/ (amel ba!ter/ /buat /

ata('a# Kaa7!aa obe! !emu/a' /taru / ata( ra! !a)at / ata( ba! e)ar'a#


15/20

Lalu, ole(a' ba!ter/ /6e'a'6/ e'6a' e)ar'a !arbol 6e't/a' /olet (elama 1

me'/t# Jat )ar'a a'6 berleb/ /bua'6, lalu /b/la( e'6a' a/r (ul/'6# Lalu,

ole(a' /6e'a'6/ e'6a' lu6ol (elama 2 me'/t# Lu6ol a'6 berleb/ /bua'6 lalu

/b/la( e'6a' a/r (ul/'6# Lalu, ole(a' /u/ e'6a' emuat al!ool :5@ tete(

em/ tete( (elama "0 et/! atau (ama/ 8at )ar'a larut, lalu /b/la( e'6a' a/r

(ul/'6# Lalu, ole(a' /6e'a'6/ e'6a' e)ar'a ta'l/'6a' a/r 9u!(/' (elama "0

et/!# Jat )ar'a a'6 berleb/ /bua'6 a' /b/la( e'6a' a/r (ul/'6 lalu

/!er/'6!a' e'6a' !erta( (ar/'6# Prearat a'6 tela (ele(a/ /)ar'a/ /tete(/

(e/!/t m/'a! emer(/, lal /er/!(a / ba)a m/!ro(!o# Mula/la e'6a'

obe!t/9 ber!e!uata' tere'a 10, Lalu /6a't/ e'6a' le'(a obe!t/9

ber!e!uata' 100# Amat/ a' 6ambar!a' a(/l'a# Ba!ter/ Gram o(/t/9 a!a'

be')ar'a u'6u a' ba!ter/ Gram 'e6at/9 a!a' ber)ar'a mera#

*I#


16/20

Gram 3 Gram 73

B. subtilis E. coli

Pe)ar'a = >G* a' A/r Fu!(/'

Prearat = Su(e'(/ ba!ter/B. subtilis a'E. coli

Perbe(ara' = 10 D 10

Ie't/9/!a(/ ba!ter/ = Gram 3 =B. subtilis

Gram 73 =E. coli

*II# Pembaa(a'

Paa ra!t/!um /'/, /la!u!a' e)ar'aa' 6ram aa ba!ter/# Pra!t/!um /'/

bertu.ua' u'tu! me'6amat/ 2 !elomo! ba!ter/ a'6 berbea, a/tu ba!ter/ 6ram

o(/t/9 a' ba!ter/ 6ram 'e6at/9 aa (amel a'6 /ber/!a' e'6a' metoe

e)ar'aa'# Pe)ar'aa' a'6 /6u'a!a' alam u./ /'/ aala e)ar'aa' e'6a'

e)ar'aa' /99ere'(/al# Pe)ar'aa' /'/ /6u'a!a' alam u./ /'/ a6ar !elomo!

ba!ter/ aa u./ aat /bea!a'#


17/20

Ster/l/(a(/ /la!u!a' a6ar ra!t/!a' a' alat7alat a'6 /6u'a!a' beba( ar/

!o'tam/'a' (e/'66a me'6ura'6/ !e(alaa' alam e'6u./a'# Setela (ter/l/(a(/

/la!u!a', ma!a (amel a'6 merua!a' (u(e'(/ amura' ba!ter/ /(/a!a'#

Pro(eur ertama a'6 /la!u!a' aala memer(/a!a' !aa ob.e! (eba6a/

temat u'tu! membuat rearat# Kaa ob.e! a'6 /6u'a!a' alam erobaa'

aru(la beba( lema!# G* aat

ber/!ata' e'6a' et/o6l/!a' aa ba!ter/ (u(e'(/# Lalu, 8at )ar'a berleb/

/bua'6 a' /b/la( e'6a' a!uae(# Tu.ua' emb/la(a' aala a6ar (/(a )ar'a

a'6 t/a! ber/!ata' e'6a' (amel (u(e'(/ ba!ter/ t/a! ter(/(a aa rearat

(e/'66a t/a! me'66a'66u e'6amata'# Lalu, rearat /6e'a'6/ e'6a' lu6ol#

Tu.ua' ember/a' lu6ol aa rearat aala a6ar membe'tu! !omle!( e'6a'

>G* a'6 /ber/!a' (ebelum'a (e/'66a >G* aat ber/!ata' leb/ !uat aa

rearat (u(e'(/ ba!ter/# Lu6ol a'6 berleb/ /bua'6 lalu /b/la( e'6a' a/r#

Tu.ua' emb/la(a' ter(ebut aala a6ar (/(a lu6ol a'6 t/a! beramur aat

terb/la(# Lalu, rearat /u/ e'6a' emuat al!ool :5@ tete( em/ tete(


18/20

(elama "0 et/! atau (ama/ 8at )ar'a larut, lalu /b/la( e'6a' a/r# Pe'u/a'

e'6a' emuat al!ool :5@ bertu.ua' a6ar >G* a'6 t/a! ber/!ata' !uat

aa rearat (u(e'(/ ba!ter/ aat terb/la(# >G* a'6 terb/la( aa ember/a'

emuat al!ool :5@ aala >G* a'6 ber/!ata' e'6a' ba!ter/ 6ram 'e6at/9

!are'a ba!ter/ 6ram 'e6at/9 mem/l/!/ et/o6l/!a' a'6 t//( (e/'66a

/!ata''a e'6a' >G* lema# e'6a' !ata la/', ba!ter/ a'6 teta mem/l/!/

)ar'a aala ba!ter/ 6ram o(/t/9# Pemb/la(a' /la!u!a' u'tu! memb/la( (/(a

emuat al!ool :5@# Lalu, rearat /6e'a'6/ e'6a' e)ar'a ta'l/'6a' a/r

9u!(/' a' (/(a )ar'a berleb/ /b/la( e'6a' a/r# Pember/a' a/r 9u!(/' bertu.ua'

u'tu! member/ )ar'a mera aa ba!ter/ 6ram 'e6at/9 (e/'66a /!etau/

aa'a ba!ter/ 6ram 'e6at/9 aa rearat# Perlu /erat/!a' ba)a alam

emb/la(a' rearat, rearat aru( /b/la( (e/!/t em/ (e/!/t e'6a' a/r#


19/20

AFTAR PUSTAKA

Fitria. 2014. Pengertian Bakteri Gram Positif dan Negatif. Tersedia di

http://www.sridianti.com/pengertian!akterigrampositifdan

negatif.htm" #$iakses 10 %aret 201&'.
http://www.sridianti.com/pengertian-bakteri-gram-positif-dan-negatif.htmlhttp://www.sridianti.com/pengertian-bakteri-gram-positif-dan-negatif.html


20/20

/ta#

&era# 2011# Laora' Pe)ar'aa' Ba!ter/# Ter(e/a /

tt(=EE)))#aaem/a#euE;1"?125ELaora'M/!roB/olo6/ /a!(e( ta'66al 10

Maret 2015H#

biotek bakteri gram

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