Identifikasi Dan Uji Aktifitas Antibakteri Senyawa Volatil Terpenoid Minyak

Terpentin

Masruri, Mohamad Farid Rahman, dan Tegas Iman Prasodjo Fakultas MIPA Universitas Brawijaya, Malang

Abstrak Diterimatanggal 5 April 2006, disetujui tanggal 8 Mei 2007

Sumber alam (naturalproducts resources) minyak terpentin di Indonesia sangat melimpah, akan

tetapi masih sangat sedikit tingkat pemanfaatannya. Tujuan dari penelitian ini adalah mencari alternatif pemanfaatan minyak terpentin sebagai bahan antimikroba. Tahapan penelitiannya adalah mengisolasi senyawa volatil terpenoid dari minyak terpentin, melakukan uji aktifitas antimikroba senyawa hasil isolasi serta mengidentiflkasi senyawa yang aktif sebagai antimikroba. Hasil penelitian diperoleh dua fraksi, dimana fraksi pertamadan kedua terbukti mampu menghambat pertumbuhan mikroba uji (aktif sebagai antimkroba). Identifikasi terhadap fraksi pertama diperoleh senyawa alfa-pinena dengan kemurnian ~ 100%. Sedangkan fraksi kedua diperoleh tiga senyawa yaitu delta-karena (91,11 %), beta-pinena (3,55%), serta alfa-terpinolena (5,34%).

Kata kunci: Alfa- dan beta-pinena; delta-karena; alfa-terpinolena, antibacterial, minyak terpentin

Identification And Antibacterial Activity Test Of Volatile Terpenoid Compounds From

Turpuntine Oils

Abstract Identification and antibacterial activity test of volatile terpenoid compounds from turpuntine

oils.The natural products resources of turpuntine oils in Indonesia was abundant, but it was less explorated. The objectives of this research were to investigate an alternative of turpentine oil utility such as antimicrobial agent. The methods used to achieve were isolation of volatile terpenoid by means of fractional distillation under reduced pressure. Then, antibacterial acitivity test of fraction distilat yielded by using paper diffusion methods. The last, identification of the active fraction as antibacterial by means of gas chromatography-mass spectrometry (GC-MS) and infrared spectrom-etry (IR) method. The result indicated two volatile fractions were yielded in the fractional distillation and both of them exhibited antibacterial activity. Meanwhile, structure elucidation informed that composition of first fraction was alpha-pinene (100% purity). Composition fo the second fraction were delta-carene (91,11 %), beta-pinene (3,55%), and alpha-terpinolene (5,34%), respectively.

Keywords: Alpha- and beta-pinene; delta-carene; alpha-terpinolene, antibacterial, volatile/turpentine

oil

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