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Microwave irradiation-assisted chitosan hydrolysis using cellulase enzyme 4 orang
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Bulletin of Chemical Reaction Engineering & Catalysis 1978-2993 Volume 13 No. 3, Juni 2018 Diponegoro University https://doi.org/l0.9767/bcrec.13.3.2378.466-474 https://ejoumal2.undip.ac.id/index.php/bcrec/article/view /2378 https://doi.org/l0.9767/bcrec.13.3.2378.466-474 Seo pus
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Microwave irradiation-assisted chitosan hydrolysis using cellulaseenzyme (Article) (Open Access)
, , ,
Department of Chemical Engineering, Diponegoro University, Kampus Undip Tembalang, Jl. Prof. Soedarto,Semarang, 50239, IndonesiaDepartment of Environmental Engineering, Diponegoro University, Kampus Undip Tembalang, Jl. Prof. Soedarto,
Semarang, 50239, Indonesia
AbstractThe influence of microwave irradiation on the chitosan hydrolysis catalyzed by cellulase enzyme was studied. Thehydrolyzed chitosan was characterized by measuring its viscosity and reducing sugar. Further, it was also characterizedby Fourier-Transform Infrared Spectroscopy (FT-IR), X-ray Diffraction (XRD), and Scanning Electron Microscope (SEM).The classical Michaelis-Menten kinetic parameters were measured by analyzing the amount of reducing sugars. Theresults were compared with the hydrolysis by using conventional shaker incubator. The hydrolysis reaction timeneeded to obtain similar reducing sugar yield was significantly lower for microwave irradiation than shaker incubator.On the other hand, the reduction rate of the relative viscosity was significantly higher for the hydrolysis of chitosanusing shaker incubator. A significant difference in chemical structure was observed between hydrolysis usingmicrowave irradiation and shaker incubator. Overall, the result showed that the hydrolysis behavior of chitosan usingmicrowave irradiation is significantly different with using shaker incubator. Copyright © 2018 BCREC Group. Allrights reserved.
SciVal Topic Prominence
Topic:
Prominence percentile: 98.547
Author keywordsCellulase Chitosan Hydrolysis Microwave irradiation
Indexed keywords
Engineeringcontrolled terms:
Chitosan Enzymes Fourier transform infrared spectroscopy Hydrolysis Irradiation
Radiation Scanning electron microscopy Viscosity
Engineeringuncontrolled terms
Cellulase Cellulase enzyme Fourier transform infra red (FTIR) spectroscopy
Hydrolysis behavior Hydrolysis reaction Michaelis-Menten kinetic Reducing sugars
Relative viscosity
Engineering mainheading:
Microwave irradiation
Funding details
Funding textThis research was financially supported by the Ministry of Research, Technology, and Higher Education, Republic ofIndonesia. The authors thank Dwi Titik Apriyanti and Haqqi-yuddin Robbani for supporting the research.
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Open AccessBulletin of Chemical Reaction Engineering & CatalysisVolume 13, Issue 3, December 2018, Pages 466-474
Rokhati, N.a Pramudono, B.a Istirokhatun, T.b Susanto, H.a
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b
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Susanto, H.; Department of Chemical Engineering, Diponegoro University, Kampus Undip Tembalang, Jl. Prof.Soedarto, Semarang, Indonesia; email: © Copyright 2018 Elsevier B.V., All rights reserved.
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Microwave Irradiation-Assisted Chitosan Hydrolysis
Using Cellulase Enzyme
Nur Rokhati1, Bambang Pramudono1, Titik Istirokhatun2, Heru Susanto1*
1Department of Chemical Engineering, Diponegoro University, Jl. Prof. Soedarto, Kampus Undip
Tembalang, Semarang 50239, Indonesia 2Department of Environmental Engineering, Diponegoro University, Jl. Prof. Soedarto, Kampus Undip
Tembalang, Semarang 50239, Indonesia
Bulletin of Chemical Reaction Engineering & Catalysis, 13 (3) 2018, 466-474
Abstract
The influence of microwave irradiation on the chitosan hydrolysis catalyzed by cellulase enzyme was
studied. The hydrolyzed chitosan was characterized by measuring its viscosity and reducing sugar.
Further, it was also characterized by Fourier-Transform Infrared Spectroscopy (FT-IR), X-ray Diffrac-
tion (XRD), and Scanning Electron Microscope (SEM). The classical Michaelis-Menten kinetic parame-
ters were measured by analyzing the amount of reducing sugars. The results were compared with the
hydrolysis by using conventional shaker incubator. The hydrolysis reaction time needed to obtain simi-
lar reducing sugar yield was significantly lower for microwave irradiation than shaker incubator. On
the other hand, the reduction rate of the relative viscosity was significantly higher for the hydrolysis of
chitosan using shaker incubator. A significant difference in chemical structure was observed between
hydrolysis using microwave irradiation and shaker incubator. Overall, the result showed that the hy-
drolysis behavior of chitosan using microwave irradiation is significantly different with using shaker
incubator. Copyright © 2018 BCREC Group. All rights reserved
Keywords: Microwave Irradiation; Hydrolysis; Chitosan; Cellulase
How to Cite: Rokhati, N., Pramudono, B., Istirokhatun, T., Susanto, H. (2018). Microwave Irradiation-
Assisted Chitosan Hydrolysis Using Cellulase Enzyme. Bulletin of Chemical Reaction Engineering &
Catalysis, 13 (3): 466-474 (doi:10.9767/bcrec.13.3.2378.466-474)
Permalink/DOI: https://doi.org/10.9767/bcrec.13.3.2378.466-474
bcrec_2378_2018 Copyright © 2018, BCREC, ISSN 1978-2993
Available online at BCREC Website: https://bcrec.undip.ac.id
Research Article
1. Introduction
Chitosan is a natural polysaccharide com-
posed of D-glucosamine and N-acetyl-D-
glucosamine with β (1-4) linkage. Unlike most
polysaccharides, the presence of free electron
pair of the amine group, which acts as a cation
allows chitosan for binding to compounds that
are anionic by an ionic or a hydrogen bond [1,2].
Chitosan has the properties of biocompatibility,
degradable, bioactivity, and non-toxicity. Low
molecular weight chitosan (LMWC) has been
widely used in many fields, such as food [3], ag-
riculture [4], biomaterials [5], and pharmaceuti-
cal [6,7] industries. Chitosan is synthesized by
deacetylation of the chitin. In general, chitosan
has high molecular weight, causing poor solubil-
ity at neutral pH and high viscosity aqueous so-
lutions. This property inhibits wider application
of chitosan.
Low molecular weight chitosan (LMWC) has
been prepared by a variety of techniques, in-
cluding acid hydrolysis, oxidative degradation, * Corresponding Author.
E-mail: [email protected] (H. Susanto)
Telp: +62-24-7460058, Fax: +62-24-76480675
Received: 19th March 2018; Revised: 19th June 2018; Accepted: 25th June 2018;
Available online: 14th November 2018; Published regularly: December 2018
Available online at: http://www.undip.ac.id/bcrec
Department of Chemical Engineering,
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7/9/2019 Editorial Team
https://ejournal2.undip.ac.id/index.php/bcrec/about/editorialTeam 3/4
Prof. Dr. Arief Widjaja (ScopusID: 13003143800 (http://www.scopus.com/authid/detail.uri?authorId=13003143800) ) Depart. of Chemical Engineering, Institut Teknologi Sepuluh Nopember, Surabaya,Indonesia
Dr. Valeria Di Sarli (ScopusID: 16021366800 (http://www.scopus.com/authid/detail.uri?authorId=16021366800) ) Institute for Research on Combustion - National Research Council of Italy (CNR), PiazzaleV. Tecchio 80, 80125, Napoli, Italy
Prof. Dr. Ho-Shing Wu (ScopusID: 7405581723 (http://www.scopus.com/authid/detail.uri?authorId=7405581723) ) Department of Chemical Engineering and Material Science, Yuan-Ze University, 135 YuanTung Road, Chung Li, Taoyuan, 32003,, Taiwan
Prof. Dr. Nor Aishah Saidina Amin (ScopusID: 35489910900(http://www.scopus.com/authid/detail.uri?authorId=35489910900) ) Faculty of Chemical and Natural Resources Engineering, Universiti Teknologi Malaysia,81310 UTM Skudai, Johor, Malaysia
Prof. Dr. Toru Wakihara (ScopusID: 12789042600 (http://www.scopus.com/authid/detail.uri?authorId=12789042600) ) University of Tokyo, Department of Chemical System Engineering, Tokyo, Japan
Prof. Dr. Raghunath V. Chaudary (ScopusID: 7101777898(http://www.scopus.com/authid/detail.uri?authorId=7101777898) ) Center for Environmental Bene�cial Catalysis, Department of Chemical and PetroleumEngineering, The University of Kansas, 1501 Wakarusa Dr., Building B-Room 112B,Lawrence, KS 66047-1803,, United States
Prof. Dr. Mostafa Barigou (ScopusID: 7003356054 (http://www.scopus.com/authid/detail.uri?authorId=7003356054) ) School of Chemical Engineering, University of Birmingham; Edgbaston, Birmingham B152TT, United Kingdom
Prof. Dr. Jose Eduardo Castanheiro (ScopusID: 6506163997 (http://www.scopus.com/authid/detail.uri?authorId=6506163997) ) Department of Chemistry, University of Evora, CQE, Evora, Portugal
Prof. Dr. Sibudjing Kawi (ScopusID: 7006257898 (http://www.scopus.com/authid/detail.uri?authorId=7006257898) ) Department of Chemical and Biochemical Engineering, National University of Singapore,Singapore
Prof. Dr. Rafael Molina (ScopusID: 7202381846 (http://www.scopus.com/authid/detail.uri?authorId=7202381846) ) Estado Sólido y Catálisis Ambiental, Departamento de Química, Facultad de Ciencias,Universidad Nacional de Colombia, Carrera 30 45-03, Bogotá, D.C., Colombia
Assoc. Prof. Dr. Oki Muraza (ScopusID: 22433275900(http://www.scopus.com/authid/detail.uri?authorId=22433275900) ) CENT & Dept. of Chemical Engineering, King Fahd University of Petroleum and Minerals,PO Box 5040 Dhahran 31261 KSA, Saudi Arabia
Assoc. Prof. Dr. Rodiansono Rodiansono (ScopusID: 55785853800(http://www.scopus.com/authid/detail.uri?authorId=55785853800) ) Department of Chemistry, Lambung Mangkurat University, Indonesia
Dr. Rino R. Mukti (ScopusID: 12244105600 (http://www.scopus.com/authid/detail.uri?authorId=12244105600) ) Division of Inorganic and Physical Chemistry Faculty of Mathematics and Natural SciencesInstitut Teknologi Bandung, Jl. Ganesha no.10 Bandung 40132, Indonesia
Prof. Dr. Kusmiyati Kusmiyati (ScopusID: 7409816823(http://www.scopus.com/authid/detail.uri?authorId=7409816823) ) Dept. of Industrial Engineering, Universitas Dian Nuswantoro, Semarang, Indonesia
Associate Editors
Prof. Dr. Andri Cahyo Kumoro (ScopusID: 22980375000(http://www.scopus.com/authid/detail.uri?authorId=22980375000) ) (English Language Editor) Department of Chemical Engineering, Diponegoro University,Indonesia
Prof. Dr. Heru Susanto (ScopusID: 15835800800 (http://www.scopus.com/authid/detail.uri?authorId=15835800800) ) Department of Chemical Engineering, Diponegoro University, Indonesia
Dr. Luqman Buchori (ScopusID: 55559743300 (http://www.scopus.com/authid/detail.uri?authorId=55559743300) ) Department of Chemical Engineering, Diponegoro University, Indonesia
7/9/2019 Editorial Team
https://ejournal2.undip.ac.id/index.php/bcrec/about/editorialTeam 4/4
(https://statcounter.com/p11098754/summary/?guest=1) Visitors Statistic (https://statcounter.com/p11098754/summary/?
guest=1) (https://creativecommons.org/licenses/by-sa/4.0/)Bulletin of Chemical Reaction Engineering & Catalysis (BCREC) (/index.php/bcrec/) is published by Department of Chemical Engineering, Diponegoro University jointly withMasyarakat Katalis Indonesia - Indonesian Catalyst SocietyBulletin of Chemical Reaction Engineering & Catalysis (BCREC) (/index.php/bcrec/) is published under licensed of a Creative Commons Attribution-ShareAlike 4.0 InternationalLicense (https://creativecommons.org/licenses/by-sa/4.0/).
Copyright ©2019 Diponegoro University (http://www.undip.ac.id). Powered by Open Journal Systems (http://pkp.sfu.ca/ojs/) and Mason Publishing OJS theme(https://github.com/masonpublishing/OJS-Theme) .
Prof. Dr. Mohamad Djaeni (ScopusID: 16027817500(http://www.scopus.com/authid/detail.uri?authorId=16027817500) ) Dept. of Chemical Engineering, Faculty of Engineering, Diponegoro University,, Indonesia
Layout Editor
Teguh Riyanto Department of Chemical Engineering, Diponegoro University, Indonesia
Bulletin of Chemical Reaction Engineering & Catalysis, 13 (3), 2018, i
EDITOR-IN-CHIEF:
Prof. Dr. I. Istadi, Department of Chemical Engineering, Diponegoro University, Indonesia; E-mail: [email protected];
(Scopus ID: 6506850769)
REGIONAL EDITOR FOR ASIA-PACIFIC:
Prof. Dr. Hadi Nur, Ibnu Sina Institute for Fundamental Science Studies, Universiti Teknologi Malaysia, 81310 UTM Skudai,
Johor, Malaysia (ScopusID: 6602169746)
Prof. Dr. Y.H. Taufiq-Yap, Centre of Excellence for Catalysis Science and Technology, Faculty of Science, Universiti Putra
Malaysia, 43400 UPM Serdang, Selangor, Malaysia (Scopus ID: 56272773200)
REGIONAL EDITOR FOR EUROPE:
Prof. Dr. Dmitry Yu. Murzin, Laboratory of Industrial Chemistry and Reaction Engineering, Abo Akademi University;
Biskopsgatan 8 20500, Turku/Åbo, Finland (Scopus ID: 18037974700)
ASSOCIATE EDITOR:
Prof. Dr. Andri C. Kumoro, Department of Chemical Engineering, Diponegoro University, Indonesia (Scopus ID:
22980375000)
Prof. Dr. Heru Susanto, Department of Chemical Engineering, Diponegoro University, Indonesia (Scopus ID: 15835800800)
Prof. Dr. Mohammad Djaeni, Department of Chemical Engineering, Diponegoro University, Indonesia (Scopus ID:
16027817500)
Dr. Luqman Buchori, Department of Chemical Engineering, Diponegoro University, Indonesia (Scopus ID: 55559743300)
EDITORIAL TEAM
Prof. Dr. Bunjerd Jongsomjit
Department of Chemical Engineering, Chulalongkorn
University, Bangkok, Thailand (ScopusID: 6603065177)
Prof. Dr. Valeria Di Sarli
Institute for Research on Combustion - National Research
Council of Italy (CNR), Piazzale V. Tecchio 80, 80125, Napoli,
Italy (Scopus ID: 16021366800)
Prof. Dr. Jose E. Castanheiro, Dept. of Chemistry,
Universidade of Evora, CQE, Evora, Portugal (Scopus ID:
6506163997)
Prof. Dr. Rafael Molina, Estado Sólido y Catálisis
Ambiental, Departamento de Química, Facultad de Ciencias,
Universidad Nacional de Colombia, Carrera 30 45-03, Bogotá,
D.C., Colombia (Scopus ID: 7202381846)
Prof. Dr. Kalama Jalama, Dept. of Chemical Engineering,
University of Johannesburg, P.O. Box 17011, Doorrnfontein
2028, Johannesburg,, South Africa (Scopus ID: 15029880600)
Prof. Dr. Ho-Shing Wu, Dept. of Chemical Engineering &
Material Science, Yuan-Ze University, 135 Yuan Tung Road,
Chung Li, Taoyuan, 32003, Taiwan, Province of China,
(Scopus ID: 7405581723)
Dr. R. Rodiansono, Deptartment of Chemistry, Lambung
Mangkurat University, Indonesia (Scopus ID: 55785853800)
Prof. Dr. Mostafa Barigou
School of Chemical Engineering, University of Birmingham,
Edgbaston, Birmingham B15 2TT, United Kingdom, (Scopus
ID: 7003356054)
Prof. Dr. Raghunath V. Chaudhari
Center for Environmental Beneficial Catalysis, Dept. of
Chemical and Petroleum Engineering, The University of
Kansas, 1501 Wakarusa Dr., Building B-Room 112B,
Lawrence, KS 66047-1803, USA, (Scopus ID: 7101777898)
Prof. Dr. Toru Wakihara, Yokohama National University,
Graduate School of Environment and Information Sciences,
Yokohama, Japan, (Scopus ID: 12789042600)
Prof. Dr. Sibudjing Kawi
Dept. of Chemical and Biochemical Engineering, National
University of Singapore, Singapore, (Scopus ID: 7006257898)
Prof. Dr. Nor Aishah Saidina Amin
Faculty of Chemical and Natural Resources Engineering,
Universiti Teknologi Malaysia, 81310 UTM Skudai, Johor,
Malaysia, (Scopus ID: 35489910900)
Dr. Oki Muraza
CENT & Dept. of Chemical Engineering, King Fahd
University of Petroleum and Minerals, PO Box 5040
Dhahran 31261 KSA, Saudi Arabia, (Scopus ID:
22433275900)
Dr. K. Kusmiyati
Dept. of Chemical Engineering, Muhammadiyah University
of Surakarta, Pabelan, Surakarta, Indonesia (Scopus ID:
7409816823)
Prof. Dr. P. Purwanto, Dept. of Chemical Engineering,
Diponegoro University, Jln. Prof. Soedarto, Kampus Undip
Tembalang, Semarang, Indonesia (Scopus ID: 56936194800)
Dr. Rino R. Mukti
Division of Inorganic and Physical Chemistry Faculty of
Mathematics and Natural Sciences Institut Teknologi
Bandung, Jl. Ganesha no.10 Bandung 40132, Indonesia,
(Scopus ID: 12244105600)
Dr. Didi Dwi Anggoro, Department of Chemical
Engineering, Diponegoro University, Indonesia (Scopus ID:
6507823375)
Prof. Dr. S. Subagjo
Dept. of Chemical Engineering, Institut Teknologi Bandung,
Jl. Ganesha 10, Bandung, Indonesia (Scopus ID =
51462066900)
Dr. Hery Haerudin
R & D Refinery - PT. Pertamina (Persero), Jl. Raya Bekasi
Km. 20 Pulogadung Jakarta Timur 13920, Indonesia (Scopus
ID: 14524667000)
INTERNATIONAL ADVISORY EDITORIAL BOARDS
Copyright © 2018, BCREC, e-ISSN 1978-2993
Available online at BCREC Website: https://bcrec.undip.ac.id
TABLE OF CONTENTS
Bulletin of Chemical Reaction Engineering & Catalysis, 13 (3), 2018, iv
Copyright © 2018, BCREC, e-ISSN 1978-2993
Available online at BCREC Website: https://bcrec.undip.ac.id
1. Editorial Team ………………………………………………………………………………………………………….. (i)
2. Aims and Scope; Publication Information; Journal Citations and Impact Factor …………………………….. (ii)
3. Indexing and Abstracting ...…………………………………………………………………………………………….. (iii)
4. Table of Contents .. ………………………………………………………………………………………………………. (iv)
5. Impact of AlCl3 and FeCl2 Addition on Catalytic Behaviors of TiCl4/MgCl2/THF Catalysts for Ethylene Polym-
erization and Ethylene/1-Hexene Copolymerization (Niyomthai, T., Jongsomjit, B., Praserthdam, P.) ……….
(393 - 404)
6. Impact of Testing Temperature on the Structure and Catalytic Properties of Au Nanotubes Composites
(Mashentseva, A.A., Zdorovets, M.V., Borgekov, D.B.) …………………………...……………………………………...
(405 - 411)
7. An Investigation on Polymerization of Ethylene by Ziegler-Natta Catalyst in the Presence of a Promoter:
Polymerization Behavior and Polymer Microstructure (Gholami, Y., Abdouss, M., Abedi, S., Azadi, F.,
Baniani, P., Arsalanfar, M.) ………………………………………………………………………………………………….
(412 - 419)
8. Dynamic Simulation of Heterogeneous Catalysis at Particle Scale to Estimate the Kinetic Parameters for the
Pore Diffusion Model (Patan, A.K., Mekala, M., Thamida, S.K.) ……………………………………………………..
(420 - 423)
9. Synthesis, Crystal Structure, and Catalytic Activity of a Calcium(II) Complex with 4-Formylbenzene-1,3-
disulfonate-isonicotinic Acid Hydrazone (Tai, X.S., Li, P.F., Liu, L.L.) …………………………………………….
(429 - 435)
10. An Efficient Synthesis of 1,8-Dioxo-Octahydroxanthenes Derivatives Using Heterogeneous Ce-ZSM-11 Zeolite
Catalyst (Magar, R.R., Pawar, G.T., Gadekar, S.P., Lande, M.K.) .....................................................................
(436 - 446)
11. Liquefaction Behaviors of Oil Palm Frond and Bamboo in 1-Butyl-3-Methylimidazolium Chloride (Tai, Z.S.,
Asmadi, M., Ali, N.) ………………………………………………………………………………………………………….
(447 - 455)
12. Catalytic Conversion of Residual Palm Oil in Spent Bleaching Earth (SBE) By HZSM-5 Zeolite based-
Catalysts (Musa, M.L., Mat, R., Abdullah, T.A.T.) ……………………………………………………………………..
(456 - 465)
13. Microwave Irradiation-Assisted Chitosan Hydrolysis Using Cellulase Enzyme (Rokhati, N., Pramudono, B.,
Istirokhatun, T., Susanto, H.) ..................................................................................................................................
(466 - 474)
14. Comparison the New Kinetics Equation of Non-competitive Sorption Cd(II) and Zn(II) onto Green Sorbent
Horse Dung Humic Acid (HD-HA) (Basuki, R., Ngatijo, N., Santosa, S.J., Rusdiarso, B.) .................................
(475 - 488)
15. Green Biofuel Production via Catalytic Pyrolysis of Waste Cooking Oil using Malaysian Dolomite Catalyst
(Raja Shahruzzaman, R.M.H., Ali, S., Yunus, R., Yun-Hin, T.Y.) .......................................................................
(489 - 501)
16. Enhanced Visible-Light-Driven Photocatalytic Activity of ZnAl Layered Double Hydroxide by Incorporation of
Co2+ (Li, D., Fan, L., Qi, M., Shen, Y., Liu, D., Li, S.) .........................................................................................
(502 - 511)
17. Preparation of 2-Methylnaphthalene from 1-Methylnaphthalene via Catalytic Isomerization and
Crystallization (Sun, H., Sun, K., Jiang, J., Gu, Z.) ...........................................................................................
(512 - 519)
18. Catalytic Study of the Partial Oxidation Reaction of Methanol to Formaldehyde in the Vapor Phase (Peyrovi,
M.H., Parsafard, N., Hasanpour, H.) ....................................................................................................................
(520 - 528)
19. Highly Sensitive Electrocatalytic Determination of Formaldehyde Using a Ni/Ionic Liquid Modified Carbon
Nanotube Paste Electrode (Zarei, E., Jamali, M.R., Ahmadi, F.) .......................................................................
(529 - 542)
20. The Effect of Aluminum Source on Performance of Beta-Zeolite as a Support for Hydrocracking Catalyst
(Hadi, M., Aghabozorg, H.R., Bozorgzadeh, H.R., Ghasemi, M.R.) .....................................................................
(543 - 552)
21. Solid State Fermentation Parameters Effect on Cellulase Production from Empty Fruit Bunch (Wonoputri, V.,
Subiantoro, S., Kresnowati, M.T.A.P., Purwadi, R.) ............................................................................................
(553 - 559
22. Synthesis, Characterization, and Catalytic Activity of Palladium-polyvinylpyrrolidone Complex in Oxidation
of Octene-1 (Akbayeva, D.N., Bakirova, B.S., Seilkhanova, G.A., Sitzman, H.) ...................................................
(560 - 572)
23. A Straightforward Selective Acylation of Phenols over ZSM-5 towards Making Paracetamol Precursors
(Roswanda, R., Sirampun, A.D., Mukti, R.R., Mujahidin, D.) .............................................................................
(573 - 587)
24. Effect of Incorporating TiO2 Photocatalyst in PVDF Hollow Fibre Membrane for Photo-Assisted Degradation
of Methylene Blue (Abdullah, N., Ayodele, B.V., Mansor, W.N.W., Abdullah, S.) .................................................
(588 - 591)
25. Author Guidelines (2018 version) ...…………………………………………………………………...................... (App.1 - 6)
26. Copyright Transfer Agreement …..………………………………………………………………………………… (App.7 - 8)
27. Publication Ethics and Malpractice Statement …………………………………………………….................... (App.9 - 10)