sintesis-protein-lengkap-311008.ppt

8/17/2019 sintesis-protein-lengkap-311008.ppt

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BIOLOGI MOLEKULER

BIOSINTESIS PROTEIN

Oleh:

BAGIAN BIOKIMIA

FAKULTAS KEDOKTERAN UNS

2008


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MAKNA SINTESIS PROTEIN

• MEKANISME ANTIBIOTIK DALAMMEMBUNUH BAKTERI DENGAN

MENGHAMBAT SINTESIS PROTEIN

DINDING BAKTERI

• ANTIBIOTIK ADA YANG BERSIFATSELEKTIF MENGHAMBAT SINTESIS

PROTEIN DINDING BAKTERI SAJA,

TETAPI ADA YG MENGHAMBAT

KEDUANYA


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Dog! o" ole#$l!% &'olog(

P%o)$* !*h'% ge+ !)!l!h %o-e'+


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Structure of eucaryotic cells

mitochondria

cell membranelysosomes

Golgi

rEPR (ribosomes)

sEPRcentrosome

nucleolus

nucleus

cytoplasm


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Fungsi organela sel

organelle major functions

cell membrane cell integrity, signal transduction

nucleus replication, transcription

nucleolus ribosome synthesis (subunits)

centrosome cell divisioncytoplasm metabolic pathways, signaling

mitochondria ATP synthesis, DNA

ribosomes protein synthesis

lysosomes intracellular degradation

rEPR protein synthesis (export)

sEPR lipid synthesis, membranogenesis

Golgi protein transport (addressing)


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Structure of mammalian genes

I1 I2

I - introns (non-coding)

promoter

enhancer

silencer

STRUCTUREREGUL-

ATION

REGUL-

ATION

E1 E2 E3

E - exons (coding)

5‘ 3‘


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S-%$#-$%!l !%!e-e%. o" ! ge+e

!+) o" ! RNA /#o!%'.o+Gene

cDNA (mRNA)

- 5‘-flanking region, structural part, 3‘-flanking region

- exons (coding) and introns (non-coding)- no real start, no real stop

- 5‘-UTR, coding region, 3‘-UTR

- no uncoding region between start and stop

- start (cap-site) and stop (poly-A tail)


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P%o#e..e. '+1ol1e) '+ ge+e

-%!+.#%'-'o+

pre-mRNA

mRNA

DNA E1 E2 E3

transcription + capping

polyadenylation

AAAAAA

splicing

AAAAA

start stop

terminationsignals

poly Asignal

CAP

start stop


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Je+'. RNA

ribosomal RNA (rRNA)

5 S, 5.8 S (160 bp), 18 S (2000 bp) and 28 S (5000 bp) RNA

ribosome structure

transfer RNA (t-RNA)small RNA species with characteristic secondary structure

(anticodon, amino acid binding)

amino acid transfer

messenger RNA (mRNA)

matrix for protein synthesis at the ribosome, different

length

translation


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S-%$*-$% RNA

AUG

(start)

UGA

(stop)

pre-mRNA

5‘ 3‘E1 E2 E3

gene

I1 I2

PAS

splicing sites

mRNA PAS

start stop5‘-UTR 3‘-UTR

AAAAAAA

CAP

TR


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S-%$*-$% RNA !)'

AAAAA C A P

TRstart

codon

stop

codon

5‘-UTR 3‘-UTR

structural part

regulatory parts


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Ge+e-'# #o)e

degenerated code: several triplets code for one amino acid,only methionine (start) has just 1 codon

stop

stop

stop


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B!h!.! ge+B!h!.! ge+

&e%.'"!-&e%.'"!-

$+'1e%.!l$+'1e%.!l

B!h!.! ge+B!h!.! ge+

&e%.'"!-&e%.'"!-$+'1e%.!l$+'1e%.!l


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KODON ATAU SANDI GENETIK

TRIPEL NUKLEOTIDA


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LIHAT 34D SINTESIS

PROTEIN

• DA! "#$

• DA! %! NT&%!% P'T&!N &N!N*& +• DA! N -A-A


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BIOSINTESIS PROTEIN• PERHATIKAN TEORI DOGMA SENTRAL, PRODUK AKHIR

GEN ADALAH PROTEIN• DNA SEBAGAI MATERI GENETIK

• RNA SEBAGAI PEMBA5A INFORMASI GENETIK

• SINTESIS PROTEIN TERJADI DI RIBOSOM

• RNA MEMBA5A 4ODON, SEDANGKAN -RNA6MEMBA5AANTI 4ODON

• PESANAN ASAM AMINO DIKODE OLEH 4ODON RNA

• ASAM AMINO YANG SATU DENGAN YANG LAINDIRANGKAI DENGAN IKATAN PEPTIDA

• START 4ODON SELALU DIMULAI DENGAN METIONINDAN SINTESIS PROTEIN DIAKHIRI DENGAN STOP 4ODON


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TABEL KODON


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Ampli.ication o. "essage by Translation

F%o 7 .l'#e) RNA ole#$le !+( %o-e'+ ole#$le. !%e !)e


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SINTESIS PROTEIN SELALU DIMULAI

DENGAN METIONIN


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TERMINASI DENGAN STOP 4ODON


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S-%$#-$%e le1el. '+ %o-e'+.

primary structure

secondary structure

tertiary structure

quatery structure

amino acid sequence

spatial arrangement of amino acids within a poly-peptide chain ( helix, ß-sheet, unstructured turns)

spatial arrangement of secondary structural elements

subunit interactions


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A'+o !#'). !%e #o+.-'-$e+-. o"

%o-e'+.

there are about 20 proteinogenic amino acids


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Proteins are "ade o. AAs #onnected

by Peptide $onds

• !/atan peptida bersi.at i/atan rang/ap

spt + lembar /ertas yang dilem (%T)

arti dipeptida, tripeptida, 0ml AA 1 i/t pep

• Pada pra/ti/um2 berwarna ungu


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I M U N O B L O T I N G

7 2 9 ; < =

=, *D!

G!&!%


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AP!3A%! 3!N!%

• 5&%T&N $'T 6NT63 D!A*N'%!%!7


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• STRUKTUR PROTEIN


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Proteins 8orm #omplex + %tructures

• *roups de.ine the

behavior o. an amino

acid and the structure

a protein domain .oldsinto9

• Acidic• $asic• Neutral• ydrophobic• ydrophilic

• &lectrostatic


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"A#A" DAN 86N*%!

P'T&!N:9 Protein egulator ;

$ioregulator( ormon)

+9 $io/atalisator ( &n9 Protein 3ontra/til2 a/tin dan miosin

?9 Protein %tru/tural2/olagen,tubulin,/eratin,gli/opr

@9 Protein Pelindung2 !g, inter.eron,

per.orin,!


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HEALTH is better

than WEALTH

HEALTH is better

than WEALTH

B'ool: D )'+', !-oge+e.'.,B'ool: D )'+', !-oge+e.'.,

%o-e'+ *!+)')!- 1!*.'+ )!+ - ge+%o-e'+ *!+)')!- 1!*.'+ )!+ - ge+

B'ool: D )'+', !-oge+e.'.,B'ool: D )'+', !-oge+e.'.,

%o-e'+ *!+)')!- 1!*.'+ )!+ - ge+%o-e'+ *!+)')!- 1!*.'+ )!+ - ge+


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Than/ you .or

your /ind

attentions 99


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TERIMA KASIH

sintesis-protein-lengkap-311008.ppt

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