Download - sterilisasi
![Page 1: sterilisasi](https://reader035.vdokumen.com/reader035/viewer/2022071708/55cf9134550346f57b8b82b5/html5/thumbnails/1.jpg)
Pertemuan ke-3
Teknik Aseptik
Kotaminanasi dapat berasal dari :1. Eksplan2. Botol Kultur3. Lingkungan kerja4. Pelaksana kegiatan
![Page 2: sterilisasi](https://reader035.vdokumen.com/reader035/viewer/2022071708/55cf9134550346f57b8b82b5/html5/thumbnails/2.jpg)
Ruang kerja
Sterilisasi lingkungan kerja
Karbol, lysol, dll
LAFC UV, Alkohol 70% , Formalin
UV ½-1 jam
![Page 3: sterilisasi](https://reader035.vdokumen.com/reader035/viewer/2022071708/55cf9134550346f57b8b82b5/html5/thumbnails/3.jpg)
Sterilisasi Alat dan AquadesScalpel, gunting, pinset, petridis, botol
kultur, erlenmeyer, kertas saring
Bungkus dengan kertas
Autoklaf, 121○C, 17.5 psi, 1 jam
![Page 4: sterilisasi](https://reader035.vdokumen.com/reader035/viewer/2022071708/55cf9134550346f57b8b82b5/html5/thumbnails/4.jpg)
Sterilisasi media
Autoklaf, 121○C, 15-17.5 psi, 20-30 menit
Sterilisasi aquadest
Autoklaf, 121○C, 15-17.5 psi, 1 jam
![Page 5: sterilisasi](https://reader035.vdokumen.com/reader035/viewer/2022071708/55cf9134550346f57b8b82b5/html5/thumbnails/5.jpg)
Beberapa jenis bahan sterilisasi eksplan beserta kisaran konsentrasi dan lama perendaman
Bahan Konsentrasi Lama perendaman
Kalsium hipoklorid 1 - 10 % 5-30 menitNatrium hipoklorid 1 – 2 % 7 – 15 menitHidrogen peroksida 3 – 10 % 5 – 15 menitGas klorin - 1 – 4 jamPerak Nitrat 1 % 5 – 30 menitMerkuri klorid 0,1 – 0,2 % 10 – 20 menitBetadine 2,5 – 10 % 5 – 10 menitFungisida 2 gram/l 20 – 30 menitAntibiotika 50 mg/l 0,5 – 1 jamAlkohol 70 % 0,5 – 1 menit
![Page 6: sterilisasi](https://reader035.vdokumen.com/reader035/viewer/2022071708/55cf9134550346f57b8b82b5/html5/thumbnails/6.jpg)
Contoh tahap-tahap sterilisasi eksplan
• 1. Fungisida/antibiotik –merkuri klorid – bilas dg aquadest steril• 2. Alkohol-sodium hipoklorid-merkuri
klorid-aquadest steril• 3. Alkohol-sodium hipoklorid-
aquadest steril
![Page 7: sterilisasi](https://reader035.vdokumen.com/reader035/viewer/2022071708/55cf9134550346f57b8b82b5/html5/thumbnails/7.jpg)
Cara menggunakan Laminar Air Flow Cabinet:
1. Nyalakan UV minim 30 menit (hindarkan sinar uv dari badan dan mata).
2. masukkan alat-alat (lampu alkohol, bunsen burner; wadah alkohol; petridis steril; kertas tissue/kapas; bahan tanam; media tanam yg sudah disterilisasi/disemprot alkohol 70%)
3. Hidupkan blower4. Nyalakan lampu alkohol5. Siap digunakan
![Page 8: sterilisasi](https://reader035.vdokumen.com/reader035/viewer/2022071708/55cf9134550346f57b8b82b5/html5/thumbnails/8.jpg)
![Page 9: sterilisasi](https://reader035.vdokumen.com/reader035/viewer/2022071708/55cf9134550346f57b8b82b5/html5/thumbnails/9.jpg)
![Page 10: sterilisasi](https://reader035.vdokumen.com/reader035/viewer/2022071708/55cf9134550346f57b8b82b5/html5/thumbnails/10.jpg)
![Page 11: sterilisasi](https://reader035.vdokumen.com/reader035/viewer/2022071708/55cf9134550346f57b8b82b5/html5/thumbnails/11.jpg)