Download - Praktikum Mikroskop
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Praktikum Mikroskop
Peni KS Mutalib
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Goals
Being skilled to use microscope
To know some important principle so that
student could operate microscope: good inshort time.
Determine alternati procedure which bewanted
Making adaptor sel and taking photo!optional"
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#ssignment in Practice Microscope$anuar% &''(
). Mention the parts o a microscope*&. +hat is the unction o those parts,-. ow to optimali/e the unctions,0. +hat should be done to ma1imi/e %our 2P,
3. +hat is the 4# o %our microscope,5. #nd rom %our right and let hand side microscope,6. 2P o no. 3 and 5: what is better, +hat is %our argument,(. 7igure the tissue with low8 medium and high power
!magniication o ob9ectie )'18 0'18 and )''1"* +rite theoerall magniication )''18 0''18 )'''1.;. 2eport %our e1periences in using microscope: The steps*)'.+hat is the dierence with the steps mention in theor%,
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Micoscope 7igure
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Seeral theor%
2esoling Power
4#: 4umerical #perture
?mmersion oil ow to use =ondensor
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>ens protection and putting slidewhile
Bringing microscope
=leaning lens
7ocusing small ob9ectie power 01 andmedium )'1
Stud%ing slides with high power 0'@031
or when using immersion oil 2apikan mik setelah selesai *
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?denti% part o the microscope Do it seeral times8 think about the unction
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Dierent location o coarse and ine ad9ustment
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7ine a9ustment
t t th b t 2P
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ow to get the best 2P rom amicroscope incl. how and when to
do:
=leaning the lenses
?lluminate the specimen 7ocusing the condenser
#9ust the diagragma
Asing immersion
Setting light intensit%
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Magniication 4umeric #perture
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4# C n sin
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2esoling Power
2esolution o lens camera is reealed b% how man% lines
per inch !lpi"
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>ens resolution ?s the abilit% o a lens to ree shadow rom
& point o thing that is er% close. 2esolusion limit C ).&&D !2esolusion depend
on "
Da%a urai !C2esoling PowerE2PEs"C C).&&D !DC&sin" where C9arak ob9ek kelensa ob9ekti
How smaller how smaller RP
How larger Dhow smallerRP
How bigger
how smaller RP
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>ight source
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>ong wae length hae greaterenerg%
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ec romagne c waespectrum
http://ethel.as.arizona.edu/~collins/astro/subjects/electromag9.htmlhttp://ethel.as.arizona.edu/~collins/astro/subjects/electromag9.html -
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Step in ocusing microscope
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Step
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Step
The best 2P isual microscope &''
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The best 2P isual microscope @ &''nm
Magnification is not the same with RP
RP microscope mainly depend on obj lens (oculer-max 1x!
only magnify imagewhich is found from obj lens-max 1x"#
$culer lens didn%t ma&e better the resolusion# Higher
magnification with low resolutionimage blur which is '
function
)* n sin
RP*
+#
)
, obj
$n the objectie was wrote Plan ./0#/
(Magnification0)"
120#134ube length in mm
4hic&ness(in mm" dec& glass for which obj lensis corected dr field
RP *!21
)*
(!21"(/!/ x 1-3m
1!2
. x 1 53m .
nmn*1!6 (n immersion oil"
sin !7
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>arge !7ast" 4# s. Small !slow" 4#
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Thus8 4#:
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=ondensor
Make better 2P with making light lamp)''H o the image@orming kind
ow: with ocusing light in the shape oinerse cone8 with ape1 at the specimenand base at the ob9ectie
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Modern 2esearchMicroscope
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Tem o8 && $uni &''-: hal. )5
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4# ).0'
I
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==D camera ASI-3''
MPi1el ,