77

7. LAMPIRAN

Lampiran 1. Formulir Kuisioner Uji Sensori (Hedonic Ranking Test)

UJI RANKING HEDONIK

Nama : Tanggal :

Jenis Kelamin :

Produk : Mie Basah

Instruksi !

Dihadapan anda telah disediakan 3 buah sampel “Mie Basah”. Amatilah dan cicipi setiap

sampel secara berurutan dari kiri ke kanan. Netralkan indera pengecap anda dengan cara

berkumur – kumur terlebih dahulu SEBELUM mencicipi sampel selanjutnya

menggunakan air putih yang telah disediakan. Setelah mencicipi semua sampel, anda

diperbolehkan mengulang sesering yang anda perlukan. Penilaian menggunakan sistem

rangking dengan skor (1 = tidak suka, 2 = kurang suka, dan 3 = suka). Skor yang

diberikan antar sampe TIDAK BOLEH SAMA.

Parameter Kode Sampel

Warna

Aroma

Rasa

Tekstur

Overall

TERIMA KASIH

78

Lampiran 2. Worksheet Uji Ranking Hedonik

WORKSHEET UJI RANKING HEDONIK

Tanggal Uji : 20 Mei 2019

Jenis Sampel : Mie Basah

Identifikasi sampel: Kode

Mie basah bekatul 5%, labu kuning 20 gram A

Mie basah bekatul 10%, labu kuning 20 gram B

Mie basah bekatul 15%, labu kuning 20 gram C

Kode kombinasi urutan penyajian sampel:

ABC = 1 ABC = 7 ABC = 13 ABC = 19 ABC = 25

ACB = 2 ACB = 8 ACB = 14 ACB = 20 ACB = 26

BAC = 3 BAC = 9 BAC = 15 BAC = 21 BAC = 27

BCA = 4 BCA = 10 BCA = 16 BCA = 22 BCA = 28

CAB = 5 CAB = 11 CAB = 17 CAB = 23 CAB = 29

CBA = 6 CBA = 12 CBA = 18 CBA = 24 CBA = 30

Penyajian:

Booth Panelis Kode Sampel urutan penyajian

I 1,11,21 325 473 6881 800 640 85211 909 779 20321

II 2,12,22 215 653 9002 902 116 82712 346 019 38022

III 3,13,23 097 873 3693 539 730 19413 937 592 43523

IV 4,14,24 108 512 7254 492 297 07414 117 375 29424

V 5,15,25 333 610 9235 036 304 55515 664 284 19325

I 6,16,26 037 727 8446 101 482 23216 472 026 95626

II 7,17,27 004 628 9637 323 450 96017 908 324 67427

III 8,18,28 936 867 5728 899 235 73018 306 875 58928

IV 9,19,29 974 269 8539 694 274 10419 456 059 53829

V 10,20,30 742 468 22610 669 576 12320 046 777 95730

Rekap kode sampel:

Sampel A 325 215 873 725 610 844 004 936 269 226 640 827 539 492 304 232

450 730 694 669 779 380 592 294 664 472 324 589 059 957

Sampel B 473 900 097 108 923 727 628 572 974 742 852 116 730 074 036 101

960 235 274 123 909 346 435 375 284 956 908 306 538 777

Sampel C 688 653 369 512 333 037 963 867 853 468 800 902 194 297 555 482

323 899 104 576 203 019 937 117 193 026 674 875 456 046

79

Lampiran 3. Perhitungan Kadar Karotenoid dan Aktivitas Antioksidan dari Pasta Labu

Kuning

Kadar Karotenoid

Rumus :

𝐾𝑎𝑑𝑎𝑟 𝑘𝑎𝑟𝑜𝑡𝑒𝑛𝑜𝑖𝑑 (𝑝𝑝𝑚) =25 × 𝐴 × 383

𝑊 × 100

Keterangan :

A = absorbansi

W = Berat sampel (g)

Ulangan Absorbansi (A) Berat sampel (g) (W) Karotenoid (ppm)

1 0,0485 1,042 4,456

2 0,0501 1,016 4,721

3 0,0515 1,038 4,750

Rata - rata 4,462

Perhitungan:

Ulangan 1 𝐾𝑎𝑑𝑎𝑟 𝑘𝑎𝑟𝑜𝑡𝑒𝑛𝑜𝑖𝑑 (𝑝𝑝𝑚) =25 ×0,0485 ×383

1,042 ×100= 4,456 𝑝𝑝𝑚

Ulangan 2 𝐾𝑎𝑑𝑎𝑟 𝑘𝑎𝑟𝑜𝑡𝑒𝑛𝑜𝑖𝑑 (𝑝𝑝𝑚) =25 ×0,0501 ×383

1,016 ×100= 4,721 𝑝𝑝𝑚

Ulangan 3 𝐾𝑎𝑑𝑎𝑟 𝑘𝑎𝑟𝑜𝑡𝑒𝑛𝑜𝑖𝑑 (𝑝𝑝𝑚) =25 ×0,0515 ×383

1,038 ×100= 4,750 𝑝𝑝𝑚

Rata – Rata = 4,456+4,721+4,750

3= 4,462 𝑝𝑝𝑚

Aktivitas Antioksidan

Rumus:

% 𝑖𝑛ℎ𝑖𝑏𝑖𝑠𝑖 = [1 − (𝐴𝑡30

𝐴𝑡0)] × 100%

Keterangan:

At30 = Absorbansi sampel pada menit ke-30

At0 = Absorbansi kontrol (blanko)

80

Ulangan At30 At0 % Inhibisi

1 0,0631 0,5135 87,712

2 0,0609 0,5135 88,140

3 0,0593 0,5135 88,452

Rata - rata 88,101

Perhitungan:

Ulangan 1 % 𝑖𝑛ℎ𝑖𝑏𝑖𝑠𝑖 = [1 − (0,0631

0,5135)] × 100% = 87,721%

Ulangan 2 % 𝑖𝑛ℎ𝑖𝑏𝑖𝑠𝑖 = [1 − (0,0609

0,5135)] × 100% = 88,140%

Ulangan 3 % 𝑖𝑛ℎ𝑖𝑏𝑖𝑠𝑖 = [1 − (0,0539

0,5135)] × 100% = 88,452%

Rata – rata = 87,712 + 88,140 + 88,452

3= 88,101%

Lampiran 4. Grafik Penurunan Nilai (Δ) Kadar Karotenoid dan Aktivitas Antioksidan

pada Sampel Mie Basah Pasca Perebusan

Karotenoid

Keterangan:

Mie kontrol = Mie basah tanpa substitusi tepung bekatul dan penambahan pasta labu kuning

Mie 1 = Mie basah dengan substitusi tepung bekatul 5% dan penambahan 20 gram pasta labu kuning

Mie 2 = Mie basah dengan substitusi tepung bekatul 10% dan penambahan 20 gram pasta labu kuning

Mie 3 = Mie basah dengan substitusi tepung bekatul 15% dan penambahan 20 gram pasta labu kuning

Gambar 8. Grafik Nilai Penurunan (Δ) Kadar Karotenoid Sampel Mie Basah Pasca

Perebusan

81

Aktivitas Antioksidan

Keterangan:

Mie kontrol = Mie basah tanpa substitusi tepung bekatul dan penambahan pasta labu kuning

Mie 1 = Mie basah dengan substitusi tepung bekatul 5% dan penambahan 20 gram pasta labu kuning

Mie 2 = Mie basah dengan substitusi tepung bekatul 10% dan penambahan 20 gram pasta labu kuning

Mie 3 = Mie basah dengan substitusi tepung bekatul 15% dan penambahan 20 gram pasta labu kuning

Gambar 9. Grafik Nilai Penurunan (Δ) Aktivitas Antioksidan Sampel Mie Basah Pasca

Perebusan

82

Lampiran 5. Hasil Pengolahan Data Perangkat SPSS 13.0 for Windows Uji Normalitas

1. Uji Fisikokimia

2. Uji Sensori

Tests of Normality

,136 12 ,200* ,952 12 ,661

,125 12 ,200* ,960 12 ,790

,097 12 ,200* ,968 12 ,892

,156 12 ,200* ,929 12 ,372

,230 12 ,079 ,858 12 ,046

,166 12 ,200* ,909 12 ,206

,124 12 ,200* ,950 12 ,642

,166 12 ,200* ,947 12 ,587

,112 12 ,200* ,957 12 ,739

,141 12 ,200* ,916 12 ,251

,171 12 ,200* ,887 12 ,109

,340 12 ,000 ,639 12 ,000

,357 12 ,000 ,676 12 ,000

,204 12 ,179 ,854 12 ,041

,186 12 ,200* ,886 12 ,106

Tensile_Strength

Daya_serap_air

L

a

b

Kadar_air

Kadar_Protein

Kadar_Abu

Kadar_Lemak

Kadar_Karbohidrat

Kadar_Serat_Pangan

Karotenoid_Sblm_Rebus

Karotenoid_Ssdh_Rebus

Antioksidan_Sblm_

Rebus

Antioksidan_Ssdh_

Rebus

Statistic df Sig. Statistic df Sig.

Kolmogorov-Smirnova

Shapiro-Wilk

This is a lower bound of the true significance.*.

Lilliefors Significance Correctiona.

Tests of Normality

,222 90 ,000 ,793 90 ,000

,221 90 ,000 ,798 90 ,000

,228 90 ,000 ,793 90 ,000

,221 90 ,000 ,796 90 ,000

,227 90 ,000 ,795 90 ,000

Warna

Aroma

Rasa

Tekstur

Overall

Statistic df Sig. Statistic df Sig.

Kolmogorov-Smirnova

Shapiro-Wilk

Lilliefors Significance Correctiona.

83

Lampiran 6. Hasil Analisis Ragam Satu Arah (One Way ANOVA) dengan Uji Beda (Post

Hoc) menggunakan Uji Duncan

1. Uji Fisikokimia

ANOVA

,001 3 ,000 49,666 ,000

,000 8 ,000

,001 11

617,000 3 205,667 24,680 ,000

66,667 8 8,333

683,667 11

113,423 3 37,808 8,901 ,006

33,980 8 4,247

147,403 11

15,437 3 5,146 66,654 ,000

,618 8 ,077

16,055 11

62,460 3 20,820 6,303 ,017

26,424 8 3,303

88,884 11

26,995 3 8,998 83,206 ,000

,865 8 ,108

27,860 11

5,281 3 1,760 47,426 ,000

,297 8 ,037

5,577 11

1,284 3 ,428 19,307 ,001

,177 8 ,022

1,461 11

13,640 3 4,547 34,005 ,000

1,070 8 ,134

14,709 11

149,666 3 49,889 199,415 ,000

2,001 8 ,250

151,668 11

1,860 3 ,620 134,066 ,000

,037 8 ,005

1,897 11

288,271 3 96,090 236,708 ,000

3,248 8 ,406

291,519 11

79,216 3 26,405 272,991 ,000

,774 8 ,097

79,990 11

Between Groups

Within Groups

Total

Between Groups

Within Groups

Total

Between Groups

Within Groups

Total

Between Groups

Within Groups

Total

Between Groups

Within Groups

Total

Between Groups

Within Groups

Total

Between Groups

Within Groups

Total

Between Groups

Within Groups

Total

Between Groups

Within Groups

Total

Between Groups

Within Groups

Total

Between Groups

Within Groups

Total

Between Groups

Within Groups

Total

Between Groups

Within Groups

Total

Tensile_Strength

Daya_serap_air

L

a

b

Kadar_air

Kadar_Protein

Kadar_Abu

Kadar_Lemak

Kadar_Karbohidrat

Kadar_Serat_Pangan

Antioksidan_Sblm_

Rebus

Antioksidan_Ssdh_

Rebus

Sum of

Squares df Mean Square F Sig.

84

Uji Duncan

Tensile_Strength

Duncana

3 ,0271

3 ,0350

3 ,0439

3 ,0551

1,000 1,000 1,000 1,000

Variabel

Bekatul15%, 20 g labu

Bekatul10%, 20g labu

Bekatul5%, 20g labu

Mie Kontrol

Sig.

N 1 2 3 4

Subset for alpha = .05

Means for groups in homogeneous subsets are displayed.

Uses Harmonic Mean Sample Size = 3,000.a.

Daya_serap_air

Duncana

3 52,6667

3 61,3333

3 64,6667

3 72,6667

1,000 ,195 1,000

Variabel

Mie Kontrol

Bekatul5%, 20g labu

Bekatul10%, 20g labu

Bekatul15%, 20 g labu

Sig.

N 1 2 3

Subset for alpha = .05

Means for groups in homogeneous subsets are displayed.

Uses Harmonic Mean Sample Size = 3,000.a.

L

Duncana

3 64,3333

3 68,2300

3 71,5533

3 71,9867

1,000 ,065

Variabel

Bekatul15%, 20 g labu

Bekatul10%, 20g labu

Bekatul5%, 20g labu

Mie Kontrol

Sig.

N 1 2

Subset for alpha = .05

Means for groups in homogeneous subsets are displayed.

Uses Harmonic Mean Sample Size = 3,000.a.

a

Duncana

3 -3,3467

3 -2,0167

3 -1,5033

3 -,1800

1,000 ,053 1,000

Variabel

Mie Kontrol

Bekatul5%, 20g labu

Bekatul10%, 20g labu

Bekatul15%, 20 g labu

Sig.

N 1 2 3

Subset for alpha = .05

Means for groups in homogeneous subsets are displayed.

Uses Harmonic Mean Sample Size = 3,000.a.

85

b

Duncana

3 20,8800

3 25,7000

3 26,0667

3 26,5433

1,000 ,600

Variabel

Mie Kontrol

Bekatul5%, 20g labu

Bekatul10%, 20g labu

Bekatul15%, 20 g labu

Sig.

N 1 2

Subset for alpha = .05

Means for groups in homogeneous subsets are displayed.

Uses Harmonic Mean Sample Size = 3,000.a.

Kadar_air

Duncana

3 31,9890

3 33,9600

3 35,1037

3 36,0033

1,000 1,000 1,000 1,000

Variabel

Mie Kontrol

Bekatul5%, 20g labu

Bekatul10%, 20g labu

Bekatul15%, 20 g labu

Sig.

N 1 2 3 4

Subset for alpha = .05

Means for groups in homogeneous subsets are displayed.

Uses Harmonic Mean Sample Size = 3,000.a.

Kadar_Protein

Duncana

3 4,9967

3 5,8450

3 6,3123

3 6,7953

1,000 1,000 1,000 1,000

Variabel

Mie Kontrol

Bekatul5%, 20g labu

Bekatul10%, 20g labu

Bekatul15%, 20 g labu

Sig.

N 1 2 3 4

Subset for alpha = .05

Means for groups in homogeneous subsets are displayed.

Uses Harmonic Mean Sample Size = 3,000.a.

Kadar_Abu

Duncana

3 1,8910

3 2,3117

3 2,6097

3 2,7430

1,000 1,000 ,305

Variabel

Mie Kontrol

Bekatul5%, 20g labu

Bekatul10%, 20g labu

Bekatul15%, 20 g labu

Sig.

N 1 2 3

Subset for alpha = .05

Means for groups in homogeneous subsets are displayed.

Uses Harmonic Mean Sample Size = 3,000.a.

86

Kadar_Lemak

Duncana

3 5,0670

3 5,8930

3 6,7900

3 7,9370

1,000 1,000 1,000 1,000

Variabel

Mie Kontrol

Bekatul5%, 20g labu

Bekatul10%, 20g labu

Bekatul15%, 20 g labu

Sig.

N 1 2 3 4

Subset for alpha = .05

Means for groups in homogeneous subsets are displayed.

Uses Harmonic Mean Sample Size = 3,000.a.

Kadar_Karbohidrat

Duncana

3 46,5210

3 49,1847

3 51,9903

3 56,0563

1,000 1,000 1,000 1,000

Variabel

Bekatul15%, 20 g labu

Bekatul10%, 20g labu

Bekatul5%, 20g labu

Mie Kontrol

Sig.

N 1 2 3 4

Subset for alpha = .05

Means for groups in homogeneous subsets are displayed.

Uses Harmonic Mean Sample Size = 3,000.a.

Kadar_Serat_Pangan

Duncana

3 ,4643

3 1,0600

3 1,2900

3 1,5233

1,000 1,000 1,000 1,000

Variabel

Mie Kontrol

Bekatul5%, 20g labu

Bekatul10%, 20g labu

Bekatul15%, 20 g labu

Sig.

N 1 2 3 4

Subset for alpha = .05

Means for groups in homogeneous subsets are displayed.

Uses Harmonic Mean Sample Size = 3,000.a.

Antioksidan_Sblm_Rebus

Duncana

3 4,6087

3 11,6003

3 15,6053

3 17,3517

1,000 1,000 1,000 1,000

Variabel

Mie Kontrol

Bekatul5%, 20g labu

Bekatul10%, 20g labu

Bekatul15%, 20 g labu

Sig.

N 1 2 3 4

Subset for alpha = .05

Means for groups in homogeneous subsets are displayed.

Uses Harmonic Mean Sample Size = 3,000.a.

87

Lampiran 7. Hasil Uji Kruskal Wallis Kadar Karotenoid

Lampiran 8. Hasil Uji T (Paired Sample) Kadar Karotenoid dan Aktivitas Antioksidan

Sebelum dan Sesudah Perebusan

Antioksidan_Ssdh_Rebus

Duncana

3 2,8560

3 5,1733

3 7,9453

3 9,5550

1,000 1,000 1,000 1,000

Variabel

Mie Kontrol

Bekatul5%, 20g labu

Bekatul10%, 20g labu

Bekatul15%, 20 g labu

Sig.

N 1 2 3 4

Subset for alpha = .05

Means for groups in homogeneous subsets are displayed.

Uses Harmonic Mean Sample Size = 3,000.a.

Test Statisticsa,b

6,995 6,683

3 3

,072 ,083

Chi-Square

df

Asymp. Sig.

Karotenoid_

Sblm_Rebus

Karotenoid_

Ssdh_Rebus

Kruskal Wallis Testa.

Grouping Variable: Variabelb.

Paired Samples Test

,18875 ,12415 ,03584 ,10987 ,26763 5,266 11 ,000

5,90908 2,61113 ,75377 4,25005 7,56811 7,839 11 ,000

Karotenoid_Sblm_Rebus

- Karotenoid_Ssdh_

Rebus

Pair

1

Antioksidan_Sblm_

Rebus - Antioksidan_

Ssdh_Rebus

Pair

2

Mean Std. Deviation

Std. Error

Mean Lower Upper

95% Confidence

Interval of the

Difference

Paired Differences

t df Sig. (2-tailed)

88

Lampiran 9. Hasil Uji Kruskal Wallis dan Uji Lanjutan Mann Whitney dari Data Analisis

Sensori

WARNA

a) 1 – 2 b) 1 – 3 c) 2 – 3

AROMA

a) 1 – 2 b) 1 – 3 c) 2 – 3

OVERALL

a) 1 – 2 b) 1 – 3 c) 2 – 3

Test Statisticsa,b

11,076 9,192 1,755 1,429 6,064

2 2 2 2 2

,004 ,010 ,416 ,489 ,048

Chi-Square

df

Asymp. Sig.

Warna Aroma Rasa Tekstur Overall

Kruskal Wallis Testa.

Grouping Variable: Produkb.

Test Statisticsa

432,000

897,000

-,286

,775

Mann-Whitney U

Wilcoxon W

Z

Asymp. Sig. (2-tailed)

Warna

Grouping Variable: Produka.

Test Statisticsa

312,000

777,000

-2,226

,026

Mann-Whitney U

Wilcoxon W

Z

Asymp. Sig. (2-tailed)

Warna

Grouping Variable: Produka.

Test Statisticsa

228,000

693,000

-3,516

,000

Mann-Whitney U

Wilcoxon W

Z

Asymp. Sig. (2-tailed)

Warna

Grouping Variable: Produka.

Test Statisticsa

339,500

804,500

-1,743

,081

Mann-Whitney U

Wilcoxon W

Z

Asymp. Sig. (2-tailed)

Aroma

Grouping Variable: Produka.

Test Statisticsa

267,500

732,500

-2,868

,004

Mann-Whitney U

Wilcoxon W

Z

Asymp. Sig. (2-tailed)

Aroma

Grouping Variable: Produka.

Test Statisticsa

347,500

812,500

-1,631

,103

Mann-Whitney U

Wilcoxon W

Z

Asymp. Sig. (2-tailed)

Aroma

Grouping Variable: Produka.

Test Statisticsa

435,500

900,500

-,230

,818

Mann-Whitney U

Wilcoxon W

Z

Asymp. Sig. (2-tailed)

Overall

Grouping Variable: Produka.

Test Statisticsa

313,000

778,000

-2,151

,031

Mann-Whitney U

Wilcoxon W

Z

Asymp. Sig. (2-tailed)

Overall

Grouping Variable: Produka.

Test Statisticsa

316,500

781,500

-2,095

,036

Mann-Whitney U

Wilcoxon W

Z

Asymp. Sig. (2-tailed)

Overall

Grouping Variable: Produka.

89

Lampiran 10. Hasil Uji Korelasi dari Data Analisis Fisikokimia

Correlations

1 -,892** -,971** -,988** ,987** -,952**

,000 ,000 ,000 ,000 ,000

12 12 12 12 12 12

-,892** 1 ,926** ,902** -,926** ,900**

,000 ,000 ,000 ,000 ,000

12 12 12 12 12 12

-,971** ,926** 1 ,965** -,984** ,966**

,000 ,000 ,000 ,000 ,000

12 12 12 12 12 12

-,988** ,902** ,965** 1 -,988** ,965**

,000 ,000 ,000 ,000 ,000

12 12 12 12 12 12

,987** -,926** -,984** -,988** 1 -,972**

,000 ,000 ,000 ,000 ,000

12 12 12 12 12 12

-,952** ,900** ,966** ,965** -,972** 1

,000 ,000 ,000 ,000 ,000

12 12 12 12 12 12

Pearson Correlation

Sig. (2-tailed)

N

Pearson Correlation

Sig. (2-tailed)

N

Pearson Correlation

Sig. (2-tailed)

N

Pearson Correlation

Sig. (2-tailed)

N

Pearson Correlation

Sig. (2-tailed)

N

Pearson Correlation

Sig. (2-tailed)

N

Tensile_Strength

Daya_serap_air

Kadar_air

Kadar_Protein

Kadar_Karbohidrat

Kadar_Serat_Pangan

Tensile_

Strength

Daya_serap_

air Kadar_air Kadar_Protein

Kadar_

Karbohidrat

Kadar_Serat_

Pangan

Correlation is significant at the 0.01 level (2-tai led).**.

90